Status
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Gene information
General information
TC034332
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012430,TC012431 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7245920..7313784
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034332
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01977

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    ovary size decreased - Number of animals showing the phenotype: 3
    egg chamber mostly not present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    iBeetle screen iB_06453

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034332 RA
      MSKTSTRIP TFRPKTPQI KPKPAEISQ KDLGKRVRV 
VEKEGTLRY VGNVHFSTG VWCGVELDS TLGKHDGAV 
NGVRYFACS QRCGLMAPL CKVALLEGT ESAEDGDSG 
PYSMLFIDH KTERDSSEE SIDDLNRTV INVKEQIKR 
TFSKNDAVS NDLYVDNGQ KFDDTDQQS YENFFNELG 
VEVKRRKTA AKNDTIVLE KPSSLPQLR TNQTFDFGV 
LSALSKFSH STPVRGDFE ESLLRKLQL TSNLDDDGS 
KRDSLEFEE SLGILTPEQ MVDGTSFMS VINSRTPSS 
ECIREIKEK PSELPSPNL VDASVTDFS LGIIDEQLI 
NNITLKTDT TMNMELPLD PVGKAVTLS RPEQTPSPE 
ELPLDPTPI VESDSKTEP TKSKTSSFI TSITSITSL 
DTGYQGDGE MSRPASRGA DNSPLTRRP LPRPQTRRP 
DPMTDSDFY TESDADNHE ENPLRGDRR AQVIDGTLY 
GVDPQAAAD IYVNNRENM DSSGIFTDI ESNPRNEED 
VRTTDVSPS DTSTKTISG NSQNNLQEI LEKSATAEE 
EVAKENPKK RNAPSPVVS SPASVRSPR HKEENASKK 
FKLPKREVA SKVKAMLES SPQSTEKKT VKKPVGRWD 
AVMNKISKT EQKTNLKDV KSKVFNSVN VGPVQRQTE 
VRKPGLRQP SSVKSPNTK LRRVRARPG STASPKSNV 
ESSIHSSLS DLSASGTLP KTAGSSKKR EVVQVTQVL 
TASKLPRQA SQNETKKTT PTTSLSENK PKRTPTSLK 
EKKQPLIKE CGRATVKGG RTSPVVRPP VHTRAPTKP 
VPKVAEALA VLVQHLVFN VEAYQVPVL KKQVQKLRN 
EAEEIRLRC QRLEETLAE ERVEKSRLI EDERVKCAN 
EISELVEKH RNEILELNS KQYELESRL CEENEANKS 
VLVKQNEEK LVTLKLEFQ KLQKTHEES LDILREEND 
AIREQIDEK NAQIEEIKH ESRKLKDDY EAKEMKLKE 
QLQSVKSEN HKLKNELGK VEDKFEEKL NAVNEENAR 
LREENERLL SFSNIKDKE TSIQELQSL RVVLELRQN 
EVAELRRAL AEAKQKNDI LSAAEERSA SLSARCEDL 
ELQLQRKNE QELTLIAEN KKLYDSFKE EINQNKRLC 
QHNEELKWK LKQNKEIVS KVLEQAAEE SSFNRSLLS 
ASFNERHST KQNFDRTLS FRQTSAPWK PRADDLSPP 
SSPKVKGVV EKSDSVSYV LDLDESPEI VASRIVRRS 
FRNSTPPKT TPTKSPSNK RPRMKFPLS LSASASAIL 
PSNKAEFGR SLSVRNGDL GGWASSTPK PQDDDLDLD 
LDEDELKLP ALPSEIGRK NGAQALPSP KHLAGEAMI 
SESNSEDES TSSSSVQL
    • >TC034332 RB
      MGQHPSKDK LNRSSSERI HTRERFGSF GKKGSSKKR 
EVVQVTQVL TASKLPRQA SQNETKKTT PTTSLSENK 
PKRTPTSLK EKKQPLIKE CGRATVKGG RTSPVVRPP 
VHTRAPTKP VPKVAEALA VLVQHLVFN VEAYQVPVL 
KKQVQKLRN EAEEIRLRC QRLEETLAE ERVEKSRLI 
EDERVKCAN EISELVEKH RNEILELNS KQYELESRL 
CEENEANKS VLVKQNEEK LVTLKLEFQ KLQKTHEES 
LDILREEND AIREQIDEK NAQIEEIKH ESRKLKDDY 
EAKEMKLKE QLQSVKSEN HKLKNELGK VEDKFEEKL 
NAVNEENAR LREENERLL SFSNIKDKE TSIQELQSL 
RVVLELRQN EVAELRRAL AEAKQKNDI LSAAEERSA 
SLSARCEDL ELQLQRKNE QELTLIAEN KKLYDSFKE 
EINQNKRLC QHNEELKWK LKQNKEIVS KVLEQAAEE 
SSFNRSLLS ASFNERHST KQNFDRTLS FRQTSAPWK 
PRADDLSPP SSPKVKGVV EKSDSVSYV LDLDESPEI 
VASRIVRRS FRNSTPPKT TPTKSPSNK RPRMKFPLS 
LSASASAIL PSNKAEFGR SLSVRNGDL GGWASSTPK 
PQDDDLDLD LDEDELKLP ALPSEIGRK NGAQALPSP 
KHLAGEAMI SESNSEDES TSSSSVQL
    CDS sequence
    • >TC034332 RA
      atgtcgaag acgtcgacc cggattccg acgtttcgg 
cccaagaca ccgcaaatc aagccgaaa ccggcggaa 
atctcgcaa aaggacctg gggaagcgg gtccgcgtg 
gtcgagaag gagggaact ctgcggtac gtcggcaac 
gtgcacttc tccacgggt gtatggtgt ggtgtcgaa 
ctcgactcg accttgggc aagcacgat ggcgccgtt 
aacggggtt cgctacttc gcctgttcg cagcgctgc 
ggcttgatg gcgccgctg tgcaaagtc gcgctgttg 
gaaggaacc gaaagtgct gaagatggg gattctggg 
ccttacagc atgctcttc atcgaccac aaaaccgag 
cgagattcg tccgaggag tcgatcgat gatctgaac 
cgaaccgtc atcaacgtc aaagagcag atcaagcgc 
acttttagc aaaaacgac gcggtctcg aacgacctg 
tacgtagac aatggtcaa aagttcgac gatacggac 
cagcagtct tacgagaac ttcttcaac gagctgggg 
gtcgaggtc aagaggcgg aaaaccgcg gcgaagaac 
gacaccatc gtcttagag aaaccgtcg tcgctgccg 
caactgcgg acgaaccaa acgttcgat ttcggcgtc 
ctgtcggcg ttgagcaaa ttcagccac tcgaccccc 
gtccgtggc gacttcgag gagtctctg ctgcggaaa 
ctgcaactg acttcgaac ctggacgac gacgggagc 
aaacgcgac tcgctggag ttcgaggag tctttgggg 
atcctcacc ccggagcag atggtcgat gggacgagc 
ttcatgtct gtgataaac tcccgaacg cctagctcc 
gaatgcatt agagagatc aaggagaag ccgagcgag 
ctcccaagc cccaacctg gtggacgcg agcgtcacc 
gacttcagc ctcgggatc atcgacgag cagctgatc 
aacaacatt acgctcaaa accgacacc accatgaac 
atggagctg cctctggac cccgtcggc aaggcggtc 
acgctgtcg cgccccgag cagacgccc tcgcccgag 
gagctgcct ctggacccg accccaatc gtggaaagc 
gactccaag accgagccg accaagtcc aaaacttcc 
agctttatt acgagtatt acgagcatt actagtctg 
gatacgggg taccagggc gatggggag atgtcccgg 
ccggcgagt cggggggct gataactcg ccactgacg 
cggcgcccc ctgccgagg ccacaaacg cgccggccg 
gaccccatg acagactcc gatttttac accgaaagc 
gatgctgat aaccacgag gagaacccc ctgcggggc 
gaccgacgg gcgcaggtc atcgacggc accctctac 
ggggttgac ccgcaagcg gcggccgac atttacgtc 
aacaaccgg gagaatatg gactcgagt ggcattttc 
accgacatt gagtcaaac cccaggaac gaggaggac 
gtcaggacg actgatgtc tccccgtcc gacacctcc 
acgaagacc atttcgggg aacagccaa aacaacctc 
caggagatt ttggaaaaa tccgcaact gctgaagaa 
gaggtcgcc aaggagaat ccgaagaag agaaatgcc 
ccgagtccg gtcgtgtcg agtcctgcg agtgtgcgg 
tcgccgagg cacaaagag gaaaatgcc tcgaaaaaa 
ttcaaattg cccaaacgc gaggtcgcc tccaaagtc 
aaggcgatg cttgagtcg agtcctcaa agcacggag 
aagaaaact gtgaaaaaa ccagtcggg cgctgggac 
gctgtgatg aataaaatc tcaaagact gaacaaaaa 
actaattta aaagacgtc aagtccaaa gtgtttaac 
agtgtgaat gtgggtccc gtgcagaga cagacagaa 
gtgaggaaa ccggggctg aggcagccg tcgagtgtg 
aagtcgccc aacaccaaa ttgcgccgg gtgagggcc 
cggcccgga agtaccgcg tctccgaag agcaacgtg 
gagagcagc atccacagc tctttgagc gacttgagt 
gcgtctggg acattgccg aagactgct gggtcgtcc 
aagaaacgc gaagtggtt caagttact caagtttta 
accgcttcg aagctacca aggcaagca tcgcaaaac 
gagactaag aagacaaca ccaacaact tccctgagt 
gaaaacaaa ccaaaacga accccgaca agccttaaa 
gagaagaag cagccatta attaaagag tgtggaaga 
gctactgtg aaggggggc cgcacgtcg cccgtagtc 
cggcccccc gttcacacg cgggccccc acgaagcct 
gtccccaaa gtggccgaa gcccttgca gtcctcgtc 
caacatctc gtgttcaat gtggaagcg tatcaagtc 
cctgtcctg aagaagcaa gtccaaaag ctgcgaaac 
gaagccgaa gaaatcagg ttgaggtgt cagcgcttg 
gaggaaact ctagccgaa gaaagggtc gagaaaagt 
cgactgatc gaagacgag cgtgtgaag tgtgcgaat 
gaaatcagc gaattagtc gagaagcat agaaatgag 
atattagaa ctgaatagc aaacaatac gagttagag 
tcgcgcctt tgcgaggaa aacgaggca aataagtcg 
gttttagtc aagcagaat gaggaaaaa ctcgtgact 
ttgaagttg gagtttcag aagttgcag aaaacgcat 
gaagagtcc ctggatatc ttacgggaa gaaaacgat 
gcgattcgt gaacagatc gacgagaag aatgcgcaa 
attgaagaa atcaaacac gagagtcgc aaactcaaa 
gacgattac gaagccaaa gagatgaag ctcaaggaa 
cagctgcag agtgttaag agtgagaat cataagttg 
aagaatgag ttgggcaag gttgaagat aagttcgag 
gagaagttg aatgcggtt aatgaggaa aacgcgagg 
ctgagggag gaaaacgaa aggctgctt agcttcagt 
aatatcaag gataaggaa actagtatt caggaactt 
cagtcactc cgagtggtc ctagaattg agacaaaac 
gaagtcgcc gagctgcgc cgcgctttg gcggaagcc 
aaacaaaaa aatgacata ttatccgca gctgaagaa 
cgatcagct tcgctcagt gccagatgc gaggatttg 
gagctccaa ttgcagcga aagaacgaa caagaactg 
accttaatt gccgaaaat aagaagtta tacgatagt 
tttaaggaa gaaatcaat caaaacaaa agactgtgt 
caacacaac gaagaactc aaatggaag ctgaagcag 
aacaaggag atcgtgagc aaagtcctg gagcaagcc 
gccgaagag tccagcttc aaccgctcc ctgctgagc 
gcaagcttc aacgagcgc cactccacc aagcaaaac 
ttcgaccgc actctctcc tttcgccaa acgagcgcg 
ccgtggaag ccgcgagcc gacgacctg tccccgccc 
tcctccccg aaggtgaag ggcgtggtc gagaagtcc 
gattcggtg agctacgtc cttgacctg gacgagagc 
cccgagatc gtggcgtcg cgcatcgtg aggcggagc 
ttccgcaac tcgactccg cccaagacc acgcccacc 
aagtcgccg tccaacaag cggccccgg atgaagttc 
cccctgagt ctgagcgcg tcggcgagc gcgattttg 
ccctcgaac aaggcggag ttcgggcgg tcgctgtcg 
gtccggaac ggggacctg gggggctgg gccagcagc 
acccccaag ccccaggac gacgacctg gacctggac 
ctggacgag gacgagctg aagctgccg gcgctgccg 
agcgagatc gggaggaag aacggggcc caggcgctg 
ccgagcccc aagcacctg gccggggag gccatgatt 
tcggagagc aattccgag gatgagagc acgagttct 
tccagtgtg caattgtga
    • >TC034332 RB
      atggggcaa catccttcg aaggataaa ctaaatcgt 
tcatcatcc gaaaggata cacactagg gagcggttc 
ggtagtttt ggcaagaaa gggtcgtcc aagaaacgc 
gaagtggtt caagttact caagtttta accgcttcg 
aagctacca aggcaagca tcgcaaaac gagactaag 
aagacaaca ccaacaact tccctgagt gaaaacaaa 
ccaaaacga accccgaca agccttaaa gagaagaag 
cagccatta attaaagag tgtggaaga gctactgtg 
aaggggggc cgcacgtcg cccgtagtc cggcccccc 
gttcacacg cgggccccc acgaagcct gtccccaaa 
gtggccgaa gcccttgca gtcctcgtc caacatctc 
gtgttcaat gtggaagcg tatcaagtc cctgtcctg 
aagaagcaa gtccaaaag ctgcgaaac gaagccgaa 
gaaatcagg ttgaggtgt cagcgcttg gaggaaact 
ctagccgaa gaaagggtc gagaaaagt cgactgatc 
gaagacgag cgtgtgaag tgtgcgaat gaaatcagc 
gaattagtc gagaagcat agaaatgag atattagaa 
ctgaatagc aaacaatac gagttagag tcgcgcctt 
tgcgaggaa aacgaggca aataagtcg gttttagtc 
aagcagaat gaggaaaaa ctcgtgact ttgaagttg 
gagtttcag aagttgcag aaaacgcat gaagagtcc 
ctggatatc ttacgggaa gaaaacgat gcgattcgt 
gaacagatc gacgagaag aatgcgcaa attgaagaa 
atcaaacac gagagtcgc aaactcaaa gacgattac 
gaagccaaa gagatgaag ctcaaggaa cagctgcag 
agtgttaag agtgagaat cataagttg aagaatgag 
ttgggcaag gttgaagat aagttcgag gagaagttg 
aatgcggtt aatgaggaa aacgcgagg ctgagggag 
gaaaacgaa aggctgctt agcttcagt aatatcaag 
gataaggaa actagtatt caggaactt cagtcactc 
cgagtggtc ctagaattg agacaaaac gaagtcgcc 
gagctgcgc cgcgctttg gcggaagcc aaacaaaaa 
aatgacata ttatccgca gctgaagaa cgatcagct 
tcgctcagt gccagatgc gaggatttg gagctccaa 
ttgcagcga aagaacgaa caagaactg accttaatt 
gccgaaaat aagaagtta tacgatagt tttaaggaa 
gaaatcaat caaaacaaa agactgtgt caacacaac 
gaagaactc aaatggaag ctgaagcag aacaaggag 
atcgtgagc aaagtcctg gagcaagcc gccgaagag 
tccagcttc aaccgctcc ctgctgagc gcaagcttc 
aacgagcgc cactccacc aagcaaaac ttcgaccgc 
actctctcc tttcgccaa acgagcgcg ccgtggaag 
ccgcgagcc gacgacctg tccccgccc tcctccccg 
aaggtgaag ggcgtggtc gagaagtcc gattcggtg 
agctacgtc cttgacctg gacgagagc cccgagatc 
gtggcgtcg cgcatcgtg aggcggagc ttccgcaac 
tcgactccg cccaagacc acgcccacc aagtcgccg 
tccaacaag cggccccgg atgaagttc cccctgagt 
ctgagcgcg tcggcgagc gcgattttg ccctcgaac 
aaggcggag ttcgggcgg tcgctgtcg gtccggaac 
ggggacctg gggggctgg gccagcagc acccccaag 
ccccaggac gacgacctg gacctggac ctggacgag 
gacgagctg aagctgccg gcgctgccg agcgagatc 
gggaggaag aacggggcc caggcgctg ccgagcccc 
aagcacctg gccggggag gccatgatt tcggagagc 
aattccgag gatgagagc acgagttct tccagtgtg 
caattgtga
    mRNA sequence
    • >TC034332 RA
      taaggtcga aaatacacc aactcgaag cgcaaactc 
gtcctaaat cgtccacct cgttcgccg attaagaat 
tttcggttt tgtattgat ccgccaaaa gataagttt 
aatgaaact gaccgtgag tgataatgt ttagacaca 
tacacgaaa tagtgaggg ctgaaatag tgtgagtga 
agcaccgac atgtcgaag acgtcgacc cggattccg 
acgtttcgg cccaagaca ccgcaaatc aagccgaaa 
ccggcggaa atctcgcaa aaggacctg gggaagcgg 
gtccgcgtg gtcgagaag gagggaact ctgcggtac 
gtcggcaac gtgcacttc tccacgggt gtatggtgt 
ggtgtcgaa ctcgactcg accttgggc aagcacgat 
ggcgccgtt aacggggtt cgctacttc gcctgttcg 
cagcgctgc ggcttgatg gcgccgctg tgcaaagtc 
gcgctgttg gaaggaacc gaaagtgct gaagatggg 
gattctggg ccttacagc atgctcttc atcgaccac 
aaaaccgag cgagattcg tccgaggag tcgatcgat 
gatctgaac cgaaccgtc atcaacgtc aaagagcag 
atcaagcgc acttttagc aaaaacgac gcggtctcg 
aacgacctg tacgtagac aatggtcaa aagttcgac 
gatacggac cagcagtct tacgagaac ttcttcaac 
gagctgggg gtcgaggtc aagaggcgg aaaaccgcg 
gcgaagaac gacaccatc gtcttagag aaaccgtcg 
tcgctgccg caactgcgg acgaaccaa acgttcgat 
ttcggcgtc ctgtcggcg ttgagcaaa ttcagccac 
tcgaccccc gtccgtggc gacttcgag gagtctctg 
ctgcggaaa ctgcaactg acttcgaac ctggacgac 
gacgggagc aaacgcgac tcgctggag ttcgaggag 
tctttgggg atcctcacc ccggagcag atggtcgat 
gggacgagc ttcatgtct gtgataaac tcccgaacg 
cctagctcc gaatgcatt agagagatc aaggagaag 
ccgagcgag ctcccaagc cccaacctg gtggacgcg 
agcgtcacc gacttcagc ctcgggatc atcgacgag 
cagctgatc aacaacatt acgctcaaa accgacacc 
accatgaac atggagctg cctctggac cccgtcggc 
aaggcggtc acgctgtcg cgccccgag cagacgccc 
tcgcccgag gagctgcct ctggacccg accccaatc 
gtggaaagc gactccaag accgagccg accaagtcc 
aaaacttcc agctttatt acgagtatt acgagcatt 
actagtctg gatacgggg taccagggc gatggggag 
atgtcccgg ccggcgagt cggggggct gataactcg 
ccactgacg cggcgcccc ctgccgagg ccacaaacg 
cgccggccg gaccccatg acagactcc gatttttac 
accgaaagc gatgctgat aaccacgag gagaacccc 
ctgcggggc gaccgacgg gcgcaggtc atcgacggc 
accctctac ggggttgac ccgcaagcg gcggccgac 
atttacgtc aacaaccgg gagaatatg gactcgagt 
ggcattttc accgacatt gagtcaaac cccaggaac 
gaggaggac gtcaggacg actgatgtc tccccgtcc 
gacacctcc acgaagacc atttcgggg aacagccaa 
aacaacctc caggagatt ttggaaaaa tccgcaact 
gctgaagaa gaggtcgcc aaggagaat ccgaagaag 
agaaatgcc ccgagtccg gtcgtgtcg agtcctgcg 
agtgtgcgg tcgccgagg cacaaagag gaaaatgcc 
tcgaaaaaa ttcaaattg cccaaacgc gaggtcgcc 
tccaaagtc aaggcgatg cttgagtcg agtcctcaa 
agcacggag aagaaaact gtgaaaaaa ccagtcggg 
cgctgggac gctgtgatg aataaaatc tcaaagact 
gaacaaaaa actaattta aaagacgtc aagtccaaa 
gtgtttaac agtgtgaat gtgggtccc gtgcagaga 
cagacagaa gtgaggaaa ccggggctg aggcagccg 
tcgagtgtg aagtcgccc aacaccaaa ttgcgccgg 
gtgagggcc cggcccgga agtaccgcg tctccgaag 
agcaacgtg gagagcagc atccacagc tctttgagc 
gacttgagt gcgtctggg acattgccg aagactgct 
gggtcgtcc aagaaacgc gaagtggtt caagttact 
caagtttta accgcttcg aagctacca aggcaagca 
tcgcaaaac gagactaag aagacaaca ccaacaact 
tccctgagt gaaaacaaa ccaaaacga accccgaca 
agccttaaa gagaagaag cagccatta attaaagag 
tgtggaaga gctactgtg aaggggggc cgcacgtcg 
cccgtagtc cggcccccc gttcacacg cgggccccc 
acgaagcct gtccccaaa gtggccgaa gcccttgca 
gtcctcgtc caacatctc gtgttcaat gtggaagcg 
tatcaagtc cctgtcctg aagaagcaa gtccaaaag 
ctgcgaaac gaagccgaa gaaatcagg ttgaggtgt 
cagcgcttg gaggaaact ctagccgaa gaaagggtc 
gagaaaagt cgactgatc gaagacgag cgtgtgaag 
tgtgcgaat gaaatcagc gaattagtc gagaagcat 
agaaatgag atattagaa ctgaatagc aaacaatac 
gagttagag tcgcgcctt tgcgaggaa aacgaggca 
aataagtcg gttttagtc aagcagaat gaggaaaaa 
ctcgtgact ttgaagttg gagtttcag aagttgcag 
aaaacgcat gaagagtcc ctggatatc ttacgggaa 
gaaaacgat gcgattcgt gaacagatc gacgagaag 
aatgcgcaa attgaagaa atcaaacac gagagtcgc 
aaactcaaa gacgattac gaagccaaa gagatgaag 
ctcaaggaa cagctgcag agtgttaag agtgagaat 
cataagttg aagaatgag ttgggcaag gttgaagat 
aagttcgag gagaagttg aatgcggtt aatgaggaa 
aacgcgagg ctgagggag gaaaacgaa aggctgctt 
agcttcagt aatatcaag gataaggaa actagtatt 
caggaactt cagtcactc cgagtggtc ctagaattg 
agacaaaac gaagtcgcc gagctgcgc cgcgctttg 
gcggaagcc aaacaaaaa aatgacata ttatccgca 
gctgaagaa cgatcagct tcgctcagt gccagatgc 
gaggatttg gagctccaa ttgcagcga aagaacgaa 
caagaactg accttaatt gccgaaaat aagaagtta 
tacgatagt tttaaggaa gaaatcaat caaaacaaa 
agactgtgt caacacaac gaagaactc aaatggaag 
ctgaagcag aacaaggag atcgtgagc aaagtcctg 
gagcaagcc gccgaagag tccagcttc aaccgctcc 
ctgctgagc gcaagcttc aacgagcgc cactccacc 
aagcaaaac ttcgaccgc actctctcc tttcgccaa 
acgagcgcg ccgtggaag ccgcgagcc gacgacctg 
tccccgccc tcctccccg aaggtgaag ggcgtggtc 
gagaagtcc gattcggtg agctacgtc cttgacctg 
gacgagagc cccgagatc gtggcgtcg cgcatcgtg 
aggcggagc ttccgcaac tcgactccg cccaagacc 
acgcccacc aagtcgccg tccaacaag cggccccgg 
atgaagttc cccctgagt ctgagcgcg tcggcgagc 
gcgattttg ccctcgaac aaggcggag ttcgggcgg 
tcgctgtcg gtccggaac ggggacctg gggggctgg 
gccagcagc acccccaag ccccaggac gacgacctg 
gacctggac ctggacgag gacgagctg aagctgccg 
gcgctgccg agcgagatc gggaggaag aacggggcc 
caggcgctg ccgagcccc aagcacctg gccggggag 
gccatgatt tcggagagc aattccgag gatgagagc 
acgagttct tccagtgtg caattgtga gacgaactc 
tcgttttag ttgcttaca aactgccct aatcgtgtg 
taatcattg taatgtatt ttattatgt aactttttt 
gtaatactt tttgtaagt tttttaaaa ctactatat 
tataattct ttatcgaaa aactctttt ctttcttga 
ataggttgt gatcttttt gtacatgat tagtagata 
ttacttata cacagtatt aatgttggg tcgagaagc 
ctttttagt acaatactg cttatactt aactatgat 
tggattgta taatgtgta tatttttat gtatatgat 
gccctttat tgtttttgt tcgttgttg ccctttcaa 
gtgtctatt aataattta tttagtgag tataggtga 
ggaatggtg atgtatgta ttttcaatt gttaggacc 
aacatagat ttccacttt tcatattta ttgtataaa 
gttgcaaat gttcgttta tgatcaagt taataatat 
tgtacaata aatttattg aactaaa
    • >TC034332 RB
      ttttacagt taaatgatt tttcatggt caccttcac 
tcttctgaa agaagacat ctattcgag gatgatttg 
taagactgg actttttat agaaaatta taaattatc 
gctgtcagc atcatgaca cacttaccc atctgtgat 
atttttaaa taaaattat ggttcgaat taaattttg 
aataaaaaa atggggcaa catccttcg aaggataaa 
ctaaatcgt tcatcatcc gaaaggata cacactagg 
gagcggttc ggtagtttt ggcaagaaa gggtcgtcc 
aagaaacgc gaagtggtt caagttact caagtttta 
accgcttcg aagctacca aggcaagca tcgcaaaac 
gagactaag aagacaaca ccaacaact tccctgagt 
gaaaacaaa ccaaaacga accccgaca agccttaaa 
gagaagaag cagccatta attaaagag tgtggaaga 
gctactgtg aaggggggc cgcacgtcg cccgtagtc 
cggcccccc gttcacacg cgggccccc acgaagcct 
gtccccaaa gtggccgaa gcccttgca gtcctcgtc 
caacatctc gtgttcaat gtggaagcg tatcaagtc 
cctgtcctg aagaagcaa gtccaaaag ctgcgaaac 
gaagccgaa gaaatcagg ttgaggtgt cagcgcttg 
gaggaaact ctagccgaa gaaagggtc gagaaaagt 
cgactgatc gaagacgag cgtgtgaag tgtgcgaat 
gaaatcagc gaattagtc gagaagcat agaaatgag 
atattagaa ctgaatagc aaacaatac gagttagag 
tcgcgcctt tgcgaggaa aacgaggca aataagtcg 
gttttagtc aagcagaat gaggaaaaa ctcgtgact 
ttgaagttg gagtttcag aagttgcag aaaacgcat 
gaagagtcc ctggatatc ttacgggaa gaaaacgat 
gcgattcgt gaacagatc gacgagaag aatgcgcaa 
attgaagaa atcaaacac gagagtcgc aaactcaaa 
gacgattac gaagccaaa gagatgaag ctcaaggaa 
cagctgcag agtgttaag agtgagaat cataagttg 
aagaatgag ttgggcaag gttgaagat aagttcgag 
gagaagttg aatgcggtt aatgaggaa aacgcgagg 
ctgagggag gaaaacgaa aggctgctt agcttcagt 
aatatcaag gataaggaa actagtatt caggaactt 
cagtcactc cgagtggtc ctagaattg agacaaaac 
gaagtcgcc gagctgcgc cgcgctttg gcggaagcc 
aaacaaaaa aatgacata ttatccgca gctgaagaa 
cgatcagct tcgctcagt gccagatgc gaggatttg 
gagctccaa ttgcagcga aagaacgaa caagaactg 
accttaatt gccgaaaat aagaagtta tacgatagt 
tttaaggaa gaaatcaat caaaacaaa agactgtgt 
caacacaac gaagaactc aaatggaag ctgaagcag 
aacaaggag atcgtgagc aaagtcctg gagcaagcc 
gccgaagag tccagcttc aaccgctcc ctgctgagc 
gcaagcttc aacgagcgc cactccacc aagcaaaac 
ttcgaccgc actctctcc tttcgccaa acgagcgcg 
ccgtggaag ccgcgagcc gacgacctg tccccgccc 
tcctccccg aaggtgaag ggcgtggtc gagaagtcc 
gattcggtg agctacgtc cttgacctg gacgagagc 
cccgagatc gtggcgtcg cgcatcgtg aggcggagc 
ttccgcaac tcgactccg cccaagacc acgcccacc 
aagtcgccg tccaacaag cggccccgg atgaagttc 
cccctgagt ctgagcgcg tcggcgagc gcgattttg 
ccctcgaac aaggcggag ttcgggcgg tcgctgtcg 
gtccggaac ggggacctg gggggctgg gccagcagc 
acccccaag ccccaggac gacgacctg gacctggac 
ctggacgag gacgagctg aagctgccg gcgctgccg 
agcgagatc gggaggaag aacggggcc caggcgctg 
ccgagcccc aagcacctg gccggggag gccatgatt 
tcggagagc aattccgag gatgagagc acgagttct 
tccagtgtg caattgtga gacgaactc tcgttttag 
ttgcttaca aactgccct aatcgtgtg taatcattg 
taatgtatt ttattatgt aactttttt gtaatactt 
tttgtaagt tttttaaaa ctactatat tataattct 
ttatcgaaa aactctttt ctttcttga ataggttgt 
gatcttttt gtacatgat tagtagata ttacttata 
cacagtatt aatgttggg tcgagaagc ctttttagt 
acaatactg cttatactt aactatgat tggattgta 
taatgtgta tatttttat gtatatgat gccctttat 
tgtttttgt tcgttgttg ccctttcaa gtgtctatt 
aataattta tttagtgag tataggtga ggaatggtg 
atgtatgta ttttcaatt gttaggacc aacatagat 
ttccacttt tcatattta ttgtataaa gttgcaaat 
gttcgttta tgatcaagt taataatat tgtacaata 
aatttattg aactaaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY