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Gene information
General information
TC012055 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7023490..7041987
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012055
    iBeetle screen iB_06449

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    longitudinal dorsal thoracic muscles potentially irregular - Number of animals showing the phenotype: 10

    iBeetle screen iB_07323

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012055 RA
      MDKTTSGND KTCYRVVLT GGPCGGKTT GQARLSTFF 
      ENMGWKVFR VPETASVLL SGGIKFSDL SEEEAVKFQ 
      ENLLKTMIQ IENTFFELG RSCNRNCLI ICDRGAMDA 
      SAFITKEKW EHIMKDNHW NNVELRDNR YNQIIHMVS 
      AAKGAEEFY SIEDHSCRS EGVELAREL DSNAAASWV 
      GHPYFDVID NSTDFEGKI RRMIGSVCH KIGIDTGDR 
      LLKNARKLK FLVKGPLPD ASLFPKFQD FEVVHNYLQ 
      SNSPNQVRL RKRGQKGHY SYIHTVRRQ NQPGGQVIE 
      VKTQITHRD YINLLTQKD NSHFTIYKL RRCFIYNNQ 
      YFQLDIYQP PSHPRCRGL ILLETYSAL DEASLKETL 
      PPFLQIEKE VTGNPDYSM FNLSLREDW TTSKKFCFY 
      PSGPQDTPA HDYGYEAGK DKIAGNLNG LDCKKSLKM 
      NGHADVKGK ECNGKA
    CDS sequence
    • >TC012055 RA
      atggataaa acgaccagt ggcaacgat aaaacctgt 
      tacagagtt gtcctaaca ggaggacca tgtggcgga 
      aaaacgacc ggtcaagcc aggctgagc acgtttttc 
      gaaaatatg ggatggaag gtcttcagg gtaccggag 
      accgcctca gttctacta agcggggga atcaagttc 
      agcgattta agtgaagag gaagccgtt aagttccaa 
      gagaacctc ctgaagacc atgatccag atcgagaac 
      accttcttc gagctgggg aggtcctgc aacagaaac 
      tgtctcata atttgtgat agaggtgcc atggatgct 
      tcagcattt ataaccaaa gaaaagtgg gaacacata 
      atgaaagac aaccactgg aacaacgtc gaactgcgc 
      gataaccgc tacaaccaa atcattcac atggtttcg 
      gcggccaaa ggagccgaa gagttttac tcgattgaa 
      gaccacagt tgtcgctcc gagggcgtc gagctggcc 
      cgcgagctg gactcgaac gccgccgcc tcctgggtc 
      gggcacccc tacttcgac gtcatcgac aactccacc 
      gacttcgag ggcaagatc cggcgcatg atcggctcg 
      gtgtgccac aagatcggc atcgacacc ggcgaccgc 
      ctgctcaag aacgcccgc aagctgaag ttcctggtc 
      aaagggccc ctgccggac gcctcgctg ttccccaag 
      ttccaagac ttcgaagtg gttcataac tacttgcag 
      tcgaactcg ccgaaccaa gtcaggttg cgaaagcgg 
      ggacagaaa gggcattac agttatatt catactgtg 
      aggcggcag aaccagcct ggaggacaa gtgattgaa 
      gttaaaacg cagataact caccgtgac tacatcaac 
      ctcctcacc cagaaggac aattcacac tttacgatt 
      tacaaacta cggaggtgt tttatttac aacaaccaa 
      tatttccaa cttgatatc tatcagcca ccgagtcat 
      cccaggtgc cgaggtttg atcttgctt gaaacctac 
      tcggctttg gacgaggcg agtttgaag gagacgctc 
      ccgccgttc ctccagatc gaaaaggaa gtaacagga 
      aatccggac tactccatg tttaactta tcattgcgc 
      gaggactgg acaacatcg aaaaaattc tgcttttat 
      ccttccggc ccccaggac accccagcc catgactat 
      ggctacgag gccgggaag gacaaaatt gcagggaat 
      ttgaacggt ctcgactgc aaaaagtcg ctaaaaatg 
      aacggccac gctgacgtg aagggtaaa gaatgtaac 
      ggaaaagct taa
    mRNA sequence
    • >TC012055 RA
      attccagtg tggccaccc ctctgcggc gaaactccc 
      tccccaaaa tgtaatttt atcagcgac gatgtttat 
      ttaagactc aaattcgct cacatttct ttagtcccg 
      cgtctgtgc ggtgtcgcg tgtcagcct ttgtcgctt 
      tcgcgaaat aacaaatca tgaaatcaa tatacagta 
      aaatttgat catgtaccc ggtttattt cttttagtg 
      cttacaaat tttgatttt cattcttca cctttcaca 
      acaaaatca ccttctcga atactagca aaattcaat 
      tggaaaaac taagtttcg tcaaaacac cggaaatat 
      ggataaaac gaccagtgg caacgataa aacctgtta 
      cagagttgt cctaacagg aggaccatg tggcggaaa 
      aacgaccgg tcaagccag gctgagcac gtttttcga 
      aaatatggg atggaaggt cttcagggt accggagac 
      cgcctcagt tctactaag cgggggaat caagttcag 
      cgatttaag tgaagagga agccgttaa gttccaaga 
      gaacctcct gaagaccat gatccagat cgagaacac 
      cttcttcga gctggggag gtcctgcaa cagaaactg 
      tctcataat ttgtgatag aggtgccat ggatgcttc 
      agcatttat aaccaaaga aaagtggga acacataat 
      gaaagacaa ccactggaa caacgtcga actgcgcga 
      taaccgcta caaccaaat cattcacat ggtttcggc 
      ggccaaagg agccgaaga gttttactc gattgaaga 
      ccacagttg tcgctccga gggcgtcga gctggcccg 
      cgagctgga ctcgaacgc cgccgcctc ctgggtcgg 
      gcaccccta cttcgacgt catcgacaa ctccaccga 
      cttcgaggg caagatccg gcgcatgat cggctcggt 
      gtgccacaa gatcggcat cgacaccgg cgaccgcct 
      gctcaagaa cgcccgcaa gctgaagtt cctggtcaa 
      agggcccct gccggacgc ctcgctgtt ccccaagtt 
      ccaagactt cgaagtggt tcataacta cttgcagtc 
      gaactcgcc gaaccaagt caggttgcg aaagcgggg 
      acagaaagg gcattacag ttatattca tactgtgag 
      gcggcagaa ccagcctgg aggacaagt gattgaagt 
      taaaacgca gataactca ccgtgacta catcaacct 
      cctcaccca gaaggacaa ttcacactt tacgattta 
      caaactacg gaggtgttt tatttacaa caaccaata 
      tttccaact tgatatcta tcagccacc gagtcatcc 
      caggtgccg aggtttgat cttgcttga aacctactc 
      ggctttgga cgaggcgag tttgaagga gacgctccc 
      gccgttcct ccagatcga aaaggaagt aacaggaaa 
      tccggacta ctccatgtt taacttatc attgcgcga 
      ggactggac aacatcgaa aaaattctg cttttatcc 
      ttccggccc ccaggacac cccagccca tgactatgg 
      ctacgaggc cgggaagga caaaattgc agggaattt 
      gaacggtct cgactgcaa aaagtcgct aaaaatgaa 
      cggccacgc tgacgtgaa gggtaaaga atgtaacgg 
      aaaagctta attttgatc ggttgaagg atttccata 
      atggacgct ttaaggccg ctcgtagct ctctcctaa 
      gttgtatta tgttttccg taattttga gtttcacgc 
      gtccgttat ggaggttga tctcccgac tcactttat 
      tatgtagtc ctacggtta attatttta atagttatt 
      tatgtagct tactttact ttgtagaca cagtattta 
      acgtagcgg tgataatta gaccagtat caatgttga 
      ataccagtg gcaaccaac tttcagtac tacttaatc 
      gtgtgacga actgaactt ctcaattga attttaatc 
      atccaactg aaataaatc ggaaagact gttcacttt 
      ttacgaaat gaaaattta caaagtcta tttttaaac 
      actttttac ttgtcacac aacttactt ttgcacttc 
      gaaatcgag ctcgcgatc gtagaaaca aacatgtac 
      ctgtttaga ttacttgtt gaagctttc gttgttatt 
      tcatttcct gagatgctg ttgtagatt tcgttattt 
      tatttgctg tgactttaa ttttaatcg gccgaagaa 
      attgtacaa aacacagga gagggcaat tctaaaggt 
      atttcactg tttttaatt cctttttaa taaaattta 
      tgcatttag agatataaa aggtggaaa ctctcgtgc 
      ttccactga tatataacc ccatctttg gggtttaga 
      gtgtgataa tgtatgtaa ttcttctac tcgcttaat 
      aaatgtgta cttatactg agggcaagc gcccttttt 
      atcatgaca tgttaagta ttcaggtga gtcaagggc 
      tcgaccttg cgcaacctt accaataaa tagtcacac 
      acaaccgca ttttcttca cccctttaa tcacacaat 
      tacaacttt tgattaaca taattcctc atgaccttt 
      ttccccgga acgagccct tgcgtgagc cccggccgt 
      tttttccgc ttttgcgta acagcctcc tgcctgatc 
      ttctccagc tcgtggggc gacggctca ggcaagctc 
      tggctctgg cagacgacc gactgctgc acctgctgc 
      tcgctttgc agctgttct ttcgacttt tgcaagtcc 
      gagtcgtgc gcgtacttg cagttgtgg ccaaagcga 
      cagcgcccc ttcctgtaa ttccagcag attcttttg 
      ccgtttatg accacgacg ttgtctttg gcgtcgacc 
      attttgacg tgcttttgc aaaatcgcc tctttggcg 
      ttctccgct tctgcgaac gggtttttg aaaaccgag 
      cttttgggg gcgtccggg tcggtggat gtgccgacg 
      aagtccggc ttggggagc ttgaggggc gcctttgcg 
      gggaccctg gaacatgta acagctgac ccgattttt 
      atttggttg atgttacga gcagggacc cagaaagtg 
      aaatgaagg ggaagtgcg cgagtgggg ttttaacgt 
      tacctggcg tcgggtttc acgggttct tcgtccgct 
      tcggacgca gaactcgat gacgagtcg taatcaggg 
      actagagac attctactg tctttaatt aattattta 
      caaacacaa acggatttg cgcgaattg aggttaggt 
      acgcttgtc acatttggg tgccaacct tgaaacatt 
      atcaaactt attttatcg tacgttaaa tcgtaacca 
      attaagcct attcgtttt tttttcgaa ctcgtacga 
      aacttagcc cgtttaggc aacttaacc gtcgaataa 
      ttaaaactt agaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY