Status
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Gene information
General information
TC012055 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7023490..7041987
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012055
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06449

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    Pupal morphology
    longitudinal dorsal thoracic muscles potentially irregular - Number of animals showing the phenotype: 10

    iBeetle screen iB_07323

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012055 RA
      MDKTTSGND KTCYRVVLT GGPCGGKTT GQARLSTFF 
ENMGWKVFR VPETASVLL SGGIKFSDL SEEEAVKFQ 
ENLLKTMIQ IENTFFELG RSCNRNCLI ICDRGAMDA 
SAFITKEKW EHIMKDNHW NNVELRDNR YNQIIHMVS 
AAKGAEEFY SIEDHSCRS EGVELAREL DSNAAASWV 
GHPYFDVID NSTDFEGKI RRMIGSVCH KIGIDTGDR 
LLKNARKLK FLVKGPLPD ASLFPKFQD FEVVHNYLQ 
SNSPNQVRL RKRGQKGHY SYIHTVRRQ NQPGGQVIE 
VKTQITHRD YINLLTQKD NSHFTIYKL RRCFIYNNQ 
YFQLDIYQP PSHPRCRGL ILLETYSAL DEASLKETL 
PPFLQIEKE VTGNPDYSM FNLSLREDW TTSKKFCFY 
PSGPQDTPA HDYGYEAGK DKIAGNLNG LDCKKSLKM 
NGHADVKGK ECNGKA
    CDS sequence
    • >TC012055 RA
      atggataaa acgaccagt ggcaacgat aaaacctgt 
tacagagtt gtcctaaca ggaggacca tgtggcgga 
aaaacgacc ggtcaagcc aggctgagc acgtttttc 
gaaaatatg ggatggaag gtcttcagg gtaccggag 
accgcctca gttctacta agcggggga atcaagttc 
agcgattta agtgaagag gaagccgtt aagttccaa 
gagaacctc ctgaagacc atgatccag atcgagaac 
accttcttc gagctgggg aggtcctgc aacagaaac 
tgtctcata atttgtgat agaggtgcc atggatgct 
tcagcattt ataaccaaa gaaaagtgg gaacacata 
atgaaagac aaccactgg aacaacgtc gaactgcgc 
gataaccgc tacaaccaa atcattcac atggtttcg 
gcggccaaa ggagccgaa gagttttac tcgattgaa 
gaccacagt tgtcgctcc gagggcgtc gagctggcc 
cgcgagctg gactcgaac gccgccgcc tcctgggtc 
gggcacccc tacttcgac gtcatcgac aactccacc 
gacttcgag ggcaagatc cggcgcatg atcggctcg 
gtgtgccac aagatcggc atcgacacc ggcgaccgc 
ctgctcaag aacgcccgc aagctgaag ttcctggtc 
aaagggccc ctgccggac gcctcgctg ttccccaag 
ttccaagac ttcgaagtg gttcataac tacttgcag 
tcgaactcg ccgaaccaa gtcaggttg cgaaagcgg 
ggacagaaa gggcattac agttatatt catactgtg 
aggcggcag aaccagcct ggaggacaa gtgattgaa 
gttaaaacg cagataact caccgtgac tacatcaac 
ctcctcacc cagaaggac aattcacac tttacgatt 
tacaaacta cggaggtgt tttatttac aacaaccaa 
tatttccaa cttgatatc tatcagcca ccgagtcat 
cccaggtgc cgaggtttg atcttgctt gaaacctac 
tcggctttg gacgaggcg agtttgaag gagacgctc 
ccgccgttc ctccagatc gaaaaggaa gtaacagga 
aatccggac tactccatg tttaactta tcattgcgc 
gaggactgg acaacatcg aaaaaattc tgcttttat 
ccttccggc ccccaggac accccagcc catgactat 
ggctacgag gccgggaag gacaaaatt gcagggaat 
ttgaacggt ctcgactgc aaaaagtcg ctaaaaatg 
aacggccac gctgacgtg aagggtaaa gaatgtaac 
ggaaaagct taa
    mRNA sequence
    • >TC012055 RA
      attccagtg tggccaccc ctctgcggc gaaactccc 
tccccaaaa tgtaatttt atcagcgac gatgtttat 
ttaagactc aaattcgct cacatttct ttagtcccg 
cgtctgtgc ggtgtcgcg tgtcagcct ttgtcgctt 
tcgcgaaat aacaaatca tgaaatcaa tatacagta 
aaatttgat catgtaccc ggtttattt cttttagtg 
cttacaaat tttgatttt cattcttca cctttcaca 
acaaaatca ccttctcga atactagca aaattcaat 
tggaaaaac taagtttcg tcaaaacac cggaaatat 
ggataaaac gaccagtgg caacgataa aacctgtta 
cagagttgt cctaacagg aggaccatg tggcggaaa 
aacgaccgg tcaagccag gctgagcac gtttttcga 
aaatatggg atggaaggt cttcagggt accggagac 
cgcctcagt tctactaag cgggggaat caagttcag 
cgatttaag tgaagagga agccgttaa gttccaaga 
gaacctcct gaagaccat gatccagat cgagaacac 
cttcttcga gctggggag gtcctgcaa cagaaactg 
tctcataat ttgtgatag aggtgccat ggatgcttc 
agcatttat aaccaaaga aaagtggga acacataat 
gaaagacaa ccactggaa caacgtcga actgcgcga 
taaccgcta caaccaaat cattcacat ggtttcggc 
ggccaaagg agccgaaga gttttactc gattgaaga 
ccacagttg tcgctccga gggcgtcga gctggcccg 
cgagctgga ctcgaacgc cgccgcctc ctgggtcgg 
gcaccccta cttcgacgt catcgacaa ctccaccga 
cttcgaggg caagatccg gcgcatgat cggctcggt 
gtgccacaa gatcggcat cgacaccgg cgaccgcct 
gctcaagaa cgcccgcaa gctgaagtt cctggtcaa 
agggcccct gccggacgc ctcgctgtt ccccaagtt 
ccaagactt cgaagtggt tcataacta cttgcagtc 
gaactcgcc gaaccaagt caggttgcg aaagcgggg 
acagaaagg gcattacag ttatattca tactgtgag 
gcggcagaa ccagcctgg aggacaagt gattgaagt 
taaaacgca gataactca ccgtgacta catcaacct 
cctcaccca gaaggacaa ttcacactt tacgattta 
caaactacg gaggtgttt tatttacaa caaccaata 
tttccaact tgatatcta tcagccacc gagtcatcc 
caggtgccg aggtttgat cttgcttga aacctactc 
ggctttgga cgaggcgag tttgaagga gacgctccc 
gccgttcct ccagatcga aaaggaagt aacaggaaa 
tccggacta ctccatgtt taacttatc attgcgcga 
ggactggac aacatcgaa aaaattctg cttttatcc 
ttccggccc ccaggacac cccagccca tgactatgg 
ctacgaggc cgggaagga caaaattgc agggaattt 
gaacggtct cgactgcaa aaagtcgct aaaaatgaa 
cggccacgc tgacgtgaa gggtaaaga atgtaacgg 
aaaagctta attttgatc ggttgaagg atttccata 
atggacgct ttaaggccg ctcgtagct ctctcctaa 
gttgtatta tgttttccg taattttga gtttcacgc 
gtccgttat ggaggttga tctcccgac tcactttat 
tatgtagtc ctacggtta attatttta atagttatt 
tatgtagct tactttact ttgtagaca cagtattta 
acgtagcgg tgataatta gaccagtat caatgttga 
ataccagtg gcaaccaac tttcagtac tacttaatc 
gtgtgacga actgaactt ctcaattga attttaatc 
atccaactg aaataaatc ggaaagact gttcacttt 
ttacgaaat gaaaattta caaagtcta tttttaaac 
actttttac ttgtcacac aacttactt ttgcacttc 
gaaatcgag ctcgcgatc gtagaaaca aacatgtac 
ctgtttaga ttacttgtt gaagctttc gttgttatt 
tcatttcct gagatgctg ttgtagatt tcgttattt 
tatttgctg tgactttaa ttttaatcg gccgaagaa 
attgtacaa aacacagga gagggcaat tctaaaggt 
atttcactg tttttaatt cctttttaa taaaattta 
tgcatttag agatataaa aggtggaaa ctctcgtgc 
ttccactga tatataacc ccatctttg gggtttaga 
gtgtgataa tgtatgtaa ttcttctac tcgcttaat 
aaatgtgta cttatactg agggcaagc gcccttttt 
atcatgaca tgttaagta ttcaggtga gtcaagggc 
tcgaccttg cgcaacctt accaataaa tagtcacac 
acaaccgca ttttcttca cccctttaa tcacacaat 
tacaacttt tgattaaca taattcctc atgaccttt 
ttccccgga acgagccct tgcgtgagc cccggccgt 
tttttccgc ttttgcgta acagcctcc tgcctgatc 
ttctccagc tcgtggggc gacggctca ggcaagctc 
tggctctgg cagacgacc gactgctgc acctgctgc 
tcgctttgc agctgttct ttcgacttt tgcaagtcc 
gagtcgtgc gcgtacttg cagttgtgg ccaaagcga 
cagcgcccc ttcctgtaa ttccagcag attcttttg 
ccgtttatg accacgacg ttgtctttg gcgtcgacc 
attttgacg tgcttttgc aaaatcgcc tctttggcg 
ttctccgct tctgcgaac gggtttttg aaaaccgag 
cttttgggg gcgtccggg tcggtggat gtgccgacg 
aagtccggc ttggggagc ttgaggggc gcctttgcg 
gggaccctg gaacatgta acagctgac ccgattttt 
atttggttg atgttacga gcagggacc cagaaagtg 
aaatgaagg ggaagtgcg cgagtgggg ttttaacgt 
tacctggcg tcgggtttc acgggttct tcgtccgct 
tcggacgca gaactcgat gacgagtcg taatcaggg 
actagagac attctactg tctttaatt aattattta 
caaacacaa acggatttg cgcgaattg aggttaggt 
acgcttgtc acatttggg tgccaacct tgaaacatt 
atcaaactt attttatcg tacgttaaa tcgtaacca 
attaagcct attcgtttt tttttcgaa ctcgtacga 
aacttagcc cgtttaggc aacttaacc gtcgaataa 
ttaaaactt agaaac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY