General information | |||
TC012055 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 7023490..7041987 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012055 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06449 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07323 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012055 RA
MDKTTSGND KTCYRVVLT GGPCGGKTT GQARLSTFF ENMGWKVFR VPETASVLL SGGIKFSDL SEEEAVKFQ ENLLKTMIQ IENTFFELG RSCNRNCLI ICDRGAMDA SAFITKEKW EHIMKDNHW NNVELRDNR YNQIIHMVS AAKGAEEFY SIEDHSCRS EGVELAREL DSNAAASWV GHPYFDVID NSTDFEGKI RRMIGSVCH KIGIDTGDR LLKNARKLK FLVKGPLPD ASLFPKFQD FEVVHNYLQ SNSPNQVRL RKRGQKGHY SYIHTVRRQ NQPGGQVIE VKTQITHRD YINLLTQKD NSHFTIYKL RRCFIYNNQ YFQLDIYQP PSHPRCRGL ILLETYSAL DEASLKETL PPFLQIEKE VTGNPDYSM FNLSLREDW TTSKKFCFY PSGPQDTPA HDYGYEAGK DKIAGNLNG LDCKKSLKM NGHADVKGK ECNGKA
- >TC012055 RA
atggataaa acgaccagt ggcaacgat aaaacctgt tacagagtt gtcctaaca ggaggacca tgtggcgga aaaacgacc ggtcaagcc aggctgagc acgtttttc gaaaatatg ggatggaag gtcttcagg gtaccggag accgcctca gttctacta agcggggga atcaagttc agcgattta agtgaagag gaagccgtt aagttccaa gagaacctc ctgaagacc atgatccag atcgagaac accttcttc gagctgggg aggtcctgc aacagaaac tgtctcata atttgtgat agaggtgcc atggatgct tcagcattt ataaccaaa gaaaagtgg gaacacata atgaaagac aaccactgg aacaacgtc gaactgcgc gataaccgc tacaaccaa atcattcac atggtttcg gcggccaaa ggagccgaa gagttttac tcgattgaa gaccacagt tgtcgctcc gagggcgtc gagctggcc cgcgagctg gactcgaac gccgccgcc tcctgggtc gggcacccc tacttcgac gtcatcgac aactccacc gacttcgag ggcaagatc cggcgcatg atcggctcg gtgtgccac aagatcggc atcgacacc ggcgaccgc ctgctcaag aacgcccgc aagctgaag ttcctggtc aaagggccc ctgccggac gcctcgctg ttccccaag ttccaagac ttcgaagtg gttcataac tacttgcag tcgaactcg ccgaaccaa gtcaggttg cgaaagcgg ggacagaaa gggcattac agttatatt catactgtg aggcggcag aaccagcct ggaggacaa gtgattgaa gttaaaacg cagataact caccgtgac tacatcaac ctcctcacc cagaaggac aattcacac tttacgatt tacaaacta cggaggtgt tttatttac aacaaccaa tatttccaa cttgatatc tatcagcca ccgagtcat cccaggtgc cgaggtttg atcttgctt gaaacctac tcggctttg gacgaggcg agtttgaag gagacgctc ccgccgttc ctccagatc gaaaaggaa gtaacagga aatccggac tactccatg tttaactta tcattgcgc gaggactgg acaacatcg aaaaaattc tgcttttat ccttccggc ccccaggac accccagcc catgactat ggctacgag gccgggaag gacaaaatt gcagggaat ttgaacggt ctcgactgc aaaaagtcg ctaaaaatg aacggccac gctgacgtg aagggtaaa gaatgtaac ggaaaagct taa
- >TC012055 RA
attccagtg tggccaccc ctctgcggc gaaactccc tccccaaaa tgtaatttt atcagcgac gatgtttat ttaagactc aaattcgct cacatttct ttagtcccg cgtctgtgc ggtgtcgcg tgtcagcct ttgtcgctt tcgcgaaat aacaaatca tgaaatcaa tatacagta aaatttgat catgtaccc ggtttattt cttttagtg cttacaaat tttgatttt cattcttca cctttcaca acaaaatca ccttctcga atactagca aaattcaat tggaaaaac taagtttcg tcaaaacac cggaaatat ggataaaac gaccagtgg caacgataa aacctgtta cagagttgt cctaacagg aggaccatg tggcggaaa aacgaccgg tcaagccag gctgagcac gtttttcga aaatatggg atggaaggt cttcagggt accggagac cgcctcagt tctactaag cgggggaat caagttcag cgatttaag tgaagagga agccgttaa gttccaaga gaacctcct gaagaccat gatccagat cgagaacac cttcttcga gctggggag gtcctgcaa cagaaactg tctcataat ttgtgatag aggtgccat ggatgcttc agcatttat aaccaaaga aaagtggga acacataat gaaagacaa ccactggaa caacgtcga actgcgcga taaccgcta caaccaaat cattcacat ggtttcggc ggccaaagg agccgaaga gttttactc gattgaaga ccacagttg tcgctccga gggcgtcga gctggcccg cgagctgga ctcgaacgc cgccgcctc ctgggtcgg gcaccccta cttcgacgt catcgacaa ctccaccga cttcgaggg caagatccg gcgcatgat cggctcggt gtgccacaa gatcggcat cgacaccgg cgaccgcct gctcaagaa cgcccgcaa gctgaagtt cctggtcaa agggcccct gccggacgc ctcgctgtt ccccaagtt ccaagactt cgaagtggt tcataacta cttgcagtc gaactcgcc gaaccaagt caggttgcg aaagcgggg acagaaagg gcattacag ttatattca tactgtgag gcggcagaa ccagcctgg aggacaagt gattgaagt taaaacgca gataactca ccgtgacta catcaacct cctcaccca gaaggacaa ttcacactt tacgattta caaactacg gaggtgttt tatttacaa caaccaata tttccaact tgatatcta tcagccacc gagtcatcc caggtgccg aggtttgat cttgcttga aacctactc ggctttgga cgaggcgag tttgaagga gacgctccc gccgttcct ccagatcga aaaggaagt aacaggaaa tccggacta ctccatgtt taacttatc attgcgcga ggactggac aacatcgaa aaaattctg cttttatcc ttccggccc ccaggacac cccagccca tgactatgg ctacgaggc cgggaagga caaaattgc agggaattt gaacggtct cgactgcaa aaagtcgct aaaaatgaa cggccacgc tgacgtgaa gggtaaaga atgtaacgg aaaagctta attttgatc ggttgaagg atttccata atggacgct ttaaggccg ctcgtagct ctctcctaa gttgtatta tgttttccg taattttga gtttcacgc gtccgttat ggaggttga tctcccgac tcactttat tatgtagtc ctacggtta attatttta atagttatt tatgtagct tactttact ttgtagaca cagtattta acgtagcgg tgataatta gaccagtat caatgttga ataccagtg gcaaccaac tttcagtac tacttaatc gtgtgacga actgaactt ctcaattga attttaatc atccaactg aaataaatc ggaaagact gttcacttt ttacgaaat gaaaattta caaagtcta tttttaaac actttttac ttgtcacac aacttactt ttgcacttc gaaatcgag ctcgcgatc gtagaaaca aacatgtac ctgtttaga ttacttgtt gaagctttc gttgttatt tcatttcct gagatgctg ttgtagatt tcgttattt tatttgctg tgactttaa ttttaatcg gccgaagaa attgtacaa aacacagga gagggcaat tctaaaggt atttcactg tttttaatt cctttttaa taaaattta tgcatttag agatataaa aggtggaaa ctctcgtgc ttccactga tatataacc ccatctttg gggtttaga gtgtgataa tgtatgtaa ttcttctac tcgcttaat aaatgtgta cttatactg agggcaagc gcccttttt atcatgaca tgttaagta ttcaggtga gtcaagggc tcgaccttg cgcaacctt accaataaa tagtcacac acaaccgca ttttcttca cccctttaa tcacacaat tacaacttt tgattaaca taattcctc atgaccttt ttccccgga acgagccct tgcgtgagc cccggccgt tttttccgc ttttgcgta acagcctcc tgcctgatc ttctccagc tcgtggggc gacggctca ggcaagctc tggctctgg cagacgacc gactgctgc acctgctgc tcgctttgc agctgttct ttcgacttt tgcaagtcc gagtcgtgc gcgtacttg cagttgtgg ccaaagcga cagcgcccc ttcctgtaa ttccagcag attcttttg ccgtttatg accacgacg ttgtctttg gcgtcgacc attttgacg tgcttttgc aaaatcgcc tctttggcg ttctccgct tctgcgaac gggtttttg aaaaccgag cttttgggg gcgtccggg tcggtggat gtgccgacg aagtccggc ttggggagc ttgaggggc gcctttgcg gggaccctg gaacatgta acagctgac ccgattttt atttggttg atgttacga gcagggacc cagaaagtg aaatgaagg ggaagtgcg cgagtgggg ttttaacgt tacctggcg tcgggtttc acgggttct tcgtccgct tcggacgca gaactcgat gacgagtcg taatcaggg actagagac attctactg tctttaatt aattattta caaacacaa acggatttg cgcgaattg aggttaggt acgcttgtc acatttggg tgccaacct tgaaacatt atcaaactt attttatcg tacgttaaa tcgtaacca attaagcct attcgtttt tttttcgaa ctcgtacga aacttagcc cgtttaggc aacttaacc gtcgaataa ttaaaactt agaaac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
