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Gene information
General information
TC012098 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5937737..5942834
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012098
    iBeetle screen iB_06444

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (19 dpi)
    Days post injection


    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 10

    labial palp not fused - Number of animals showing the phenotype: 4

    antenna orientation irregular - Number of animals showing the phenotype: 3

    eyes size decreased - Number of animals showing the phenotype: 10

    ommatidia partially fused - Number of animals showing the phenotype: 10
    ommatidia number decreased - Number of animals showing the phenotype: 10
    elytra shortened - Number of animals showing the phenotype: 10

    Adult morphology
    eyes irregular - Number of animals showing the phenotype: 10


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012098 RA
      MSKRHAEES TSGGPPPIK KIHFEPHLI GPVSTLEEM 
      DIKVLQFQN KKLAQRIEQ RYRTEQELR QRIEQLEKR 
      QTQDDAMLN VVNRYWNQL NEDIRILLQ RFDAETADE 
      SENKNESEA TTSFLMQLS TWDKEELND KLANRVQVS 
      RRAVAKVIQ AFDRLMQRN EKITLALKG ELEGQDAPS 
      VDEVICQTN VQLQAENRN LQALNTSLH EKYHSNSLK 
      VAELQDSVT AKETEIAEL KNQIDDLQY ELEKVRNRN 
      DKLETHLAE AIEKLKTYH QLHGDPEKG PQQKQIVQS 
      NVSQAKLED LTKEVEELT ELANTRLAE LDKLHQTHR 
      ETLKEVEKL KMDIRQLPE SVIVETTEY KCLQSQFSV 
      LYNESMQLK TQLDEARQQ LQTSKNAHL RNIEMMESE 
      ELIAQKKLR QEVMQLEDF LAQLRKEYE MLRIEFEQN 
      LAANEQTGP INREMRHLI TSLQNNTQQ LKGEVHRYK 
      RKYKEANAE IPKLKKEIE DLNAKLAQM QGASQDSKE 
      NIKKEVKQE EDASSSESG AQVKEEPTS APAVKKEED 
      ESEQTEEGD GDKASNSGN SPSAKKDAG VKQEKGAVK 
      KEPTVKTEK DHREAQRAK DAKIAESEM VRDLKNQLK 
      KALNEQKEM KLLLDMYKG VSKEQRDKV QLMAAEKKL 
      RTELEDIRQ QIKKMQDSK RDDKRKLAE DEALKKIKQ 
      LEEQKYELQ KQVASQKPS DGNWGGHNV LHQMRPFVG 
      SHEEEALLN EMEVTGQAF EDMQEQNSR LIQQLREKD 
      DANFKLMSE RIKSNQLHK LAREEKDVL KEQVSTLTT 
      QVEAANLVV RKLEEKERI LQNTLATVE KELALRQQA 
      MEMHKRKAI ESAQSAADL KLHLEKYHS QMKEAQQVV 
      AEKTSSLEA EAYKTKRLQ EEIAQLKRK AERMKKMEL 
      AGTTLDEVM MEEIREYKE TLTCPSCKV KRKDAVLSK 
      CFHVFCYDC LKTRYETRQ RKCPKCNCA FGANDYHRL 
      FLSN
    CDS sequence
    • >TC012098 RA
      atgtccaaa agacacgcc gaggagagc acgagcggg 
      ggcccgcct cccatcaag aagatccac ttcgagccc 
      catctcatc gggccggtg tccacgctc gaggagatg 
      gacatcaag gtgttgcag ttccagaac aagaaactg 
      gcccagaga attgagcaa cggtacagg acggagcaa 
      gagttgcgg caaagaatc gagcagttg gagaagagg 
      cagacacag gacgatgcc atgttgaac gttgttaat 
      aggtattgg aaccagttg aatgaggat attaggatt 
      ttgttgcaa cggtttgat gccgagacc gctgacgag 
      tcggagaat aaaaatgag agtgaggcc acaacttcg 
      tttttgatg caactttca acgtgggat aaggaggag 
      ttgaacgat aagttggct aatagggtg caggtgtcg 
      cggagggct gtggcgaag gtcatacaa gcctttgac 
      cggctgatg caaaggaac gagaaaatt actttagcg 
      cttaaaggc gagctagaa ggccaggat gcaccgagt 
      gtggatgaa gtcatttgc caaaccaac gtccagctc 
      caggctgag aaccgcaat ttgcaagcc ctcaataca 
      tctctccat gaaaaatat cactcaaat agcctcaaa 
      gttgccgaa ttgcaagac tcagtcacg gcgaaagaa 
      acagaaatt gccgaatta aaaaaccaa attgacgat 
      cttcaatac gagttggag aaagttcgc aatcgaaac 
      gacaaactt gagacacat ttagctgaa gctatcgaa 
      aaattgaaa acttatcac cagttgcat ggagatccg 
      gaaaaggga ccccaacag aaacaaatc gttcagtct 
      aatgtgtct caagccaaa cttgaggac ttgactaag 
      gaagttgag gagttgacc gaattggca aacactcga 
      ctagcagag ttggataag ttgcaccaa actcatcgc 
      gagacgttg aaagaagtt gaaaagttg aaaatggat 
      atacgacaa ctccccgaa agtgttatt gtggaaaca 
      accgaatat aagtgtctc caatcacag ttttcggta 
      ctttacaac gaatcaatg cagttgaaa acacagtta 
      gacgaggcc agacaacag ctacaaacc agcaaaaat 
      gcccatctg agaaacatt gaaatgatg gaaagtgag 
      gaactcatc gctcagaag aagctcaga caggaggtt 
      atgcaactt gaagacttc ctagctcaa ctgcgcaaa 
      gaatacgaa atgttacga atcgaattt gagcagaat 
      ctagccgcc aacgaacaa acaggtccc ataaatcgg 
      gaaatgcga cacttgatc acatcactc caaaacaac 
      acgcaacaa ttgaaaggg gaagtgcac cgctacaag 
      agaaagtat aaggaagca aacgccgaa ataccgaaa 
      ctaaagaaa gaaattgaa gatctgaac gctaaactt 
      gcccaaatg cagggcgcc agccaggat agtaaagaa 
      aacatcaag aaggaggtg aagcaggag gaagatgcg 
      tcaagtagc gaatcgggg gcgcaggtc aaggaggaa 
      ccgacttct gcaccggcg gtgaagaag gaggaagac 
      gagtcagag caaactgaa gaaggagat ggggacaaa 
      gcctcgaat tcgggcaac tcaccctct gctaaaaaa 
      gacgcgggg gttaagcag gaaaagggc gctgtgaag 
      aaggaacca actgttaag actgaaaag gaccatcgt 
      gaggcacaa agggccaaa gacgcgaaa attgccgaa 
      agtgaaatg gtgcgcgat cttaaaaat caactgaag 
      aaagcactg aacgagcaa aaagagatg aaactcctc 
      ctcgatatg tacaaaggt gtcagcaaa gaacaacgc 
      gacaaagtc caactaatg gcagccgaa aaaaagctt 
      cgtactgaa ttagaagat atccggcaa caaataaaa 
      aaaatgcaa gacagtaaa agagacgac aagagaaaa 
      ctcgccgag gacgaagcc ctcaaaaaa atcaaacaa 
      ctggaggag caaaaatac gagttgcaa aagcaagta 
      gcgtcgcaa aaaccgtcg gacggtaac tggggcggt 
      cacaacgtc ttgcaccaa atgcgaccg ttcgttggg 
      tcgcacgaa gaagaggcg cttttgaac gagatggaa 
      gtgacagga caagcgttt gaagacatg caggagcaa 
      aactcacgc ttgatccaa cagttgcgt gagaaggac 
      gacgcaaac ttcaagtta atgtcagag aggatcaaa 
      tcaaaccag ctccataaa ctggcaagg gaggagaag 
      gacgtgttg aaggagcaa gtcagcact ttgacgact 
      caagtcgag gctgccaat ttggttgtg cggaagttg 
      gaggagaag gagcggatt ctccagaac actctagcc 
      acggttgag aaggagctg gcgttgagg cagcaagcg 
      atggagatg cacaagaga aaggccatt gaaagtgcg 
      caatcagcg gccgatttg aagctccat ctcgagaaa 
      taccactcg cagatgaag gaggcgcag caagtggtt 
      gccgaaaaa acaagctct ctggaggcg gaagcctac 
      aagaccaaa cgactccaa gaggagatc gctcagttg 
      aagcgaaag gctgaaagg atgaagaag atggagctg 
      gcgggaacc acacttgat gaggtcatg atggaggaa 
      attcgcgaa tataaggag acactgact tgtccctcg 
      tgcaaagtc aagaggaaa gatgcggtt ttatccaaa 
      tgtttccac gttttttgc tacgattgt ctcaagacg 
      agatacgag acgaggcag aggaagtgt ccaaagtgc 
      aactgcgcg tttggcgcc aacgactat catcgcctg 
      tttttgtcg aattaa
    mRNA sequence
    • >TC012098 RA
      gggcttcaa agggcgaaa ttaagccaa aatttgcaa 
      attaaatgc aggaaaata gacaaacat ggcgcatgc 
      gcagtgctg ccaacaggt tacggttgt ttgccctga 
      gatcgataa ccaaaaacg agccaaaat taattaatt 
      ttttaatta ataaatgtg attaaagtg tgtggtttg 
      ttttagata aagtgtccc gtctttaat ttggggaaa 
      aaatcgcaa ctttgagaa ataaccttg attcttatt 
      gcaaatttc aatcagtgt ttgtttcta accccctag 
      tcgtgtttt tggtataaa agacgtgct accgcgatt 
      gtaacccca aaatgtcca aaagacacg ccgaggaga 
      gcacgagcg ggggcccgc ctcccatca agaagatcc 
      acttcgagc cccatctca tcgggccgg tgtccacgc 
      tcgaggaga tggacatca aggtgttgc agttccaga 
      acaagaaac tggcccaga gaattgagc aacggtaca 
      ggacggagc aagagttgc ggcaaagaa tcgagcagt 
      tggagaaga ggcagacac aggacgatg ccatgttga 
      acgttgtta ataggtatt ggaaccagt tgaatgagg 
      atattagga ttttgttgc aacggtttg atgccgaga 
      ccgctgacg agtcggaga ataaaaatg agagtgagg 
      ccacaactt cgtttttga tgcaacttt caacgtggg 
      ataaggagg agttgaacg ataagttgg ctaataggg 
      tgcaggtgt cgcggaggg ctgtggcga aggtcatac 
      aagcctttg accggctga tgcaaagga acgagaaaa 
      ttactttag cgcttaaag gcgagctag aaggccagg 
      atgcaccga gtgtggatg aagtcattt gccaaacca 
      acgtccagc tccaggctg agaaccgca atttgcaag 
      ccctcaata catctctcc atgaaaaat atcactcaa 
      atagcctca aagttgccg aattgcaag actcagtca 
      cggcgaaag aaacagaaa ttgccgaat taaaaaacc 
      aaattgacg atcttcaat acgagttgg agaaagttc 
      gcaatcgaa acgacaaac ttgagacac atttagctg 
      aagctatcg aaaaattga aaacttatc accagttgc 
      atggagatc cggaaaagg gaccccaac agaaacaaa 
      tcgttcagt ctaatgtgt ctcaagcca aacttgagg 
      acttgacta aggaagttg aggagttga ccgaattgg 
      caaacactc gactagcag agttggata agttgcacc 
      aaactcatc gcgagacgt tgaaagaag ttgaaaagt 
      tgaaaatgg atatacgac aactccccg aaagtgtta 
      ttgtggaaa caaccgaat ataagtgtc tccaatcac 
      agttttcgg tactttaca acgaatcaa tgcagttga 
      aaacacagt tagacgagg ccagacaac agctacaaa 
      ccagcaaaa atgcccatc tgagaaaca ttgaaatga 
      tggaaagtg aggaactca tcgctcaga agaagctca 
      gacaggagg ttatgcaac ttgaagact tcctagctc 
      aactgcgca aagaatacg aaatgttac gaatcgaat 
      ttgagcaga atctagccg ccaacgaac aaacaggtc 
      ccataaatc gggaaatgc gacacttga tcacatcac 
      tccaaaaca acacgcaac aattgaaag gggaagtgc 
      accgctaca agagaaagt ataaggaag caaacgccg 
      aaataccga aactaaaga aagaaattg aagatctga 
      acgctaaac ttgcccaaa tgcagggcg ccagccagg 
      atagtaaag aaaacatca agaaggagg tgaagcagg 
      aggaagatg cgtcaagta gcgaatcgg gggcgcagg 
      tcaaggagg aaccgactt ctgcaccgg cggtgaaga 
      aggaggaag acgagtcag agcaaactg aagaaggag 
      atggggaca aagcctcga attcgggca actcaccct 
      ctgctaaaa aagacgcgg gggttaagc aggaaaagg 
      gcgctgtga agaaggaac caactgtta agactgaaa 
      aggaccatc gtgaggcac aaagggcca aagacgcga 
      aaattgccg aaagtgaaa tggtgcgcg atcttaaaa 
      atcaactga agaaagcac tgaacgagc aaaaagaga 
      tgaaactcc tcctcgata tgtacaaag gtgtcagca 
      aagaacaac gcgacaaag tccaactaa tggcagccg 
      aaaaaaagc ttcgtactg aattagaag atatccggc 
      aacaaataa aaaaaatgc aagacagta aaagagacg 
      acaagagaa aactcgccg aggacgaag ccctcaaaa 
      aaatcaaac aactggagg agcaaaaat acgagttgc 
      aaaagcaag tagcgtcgc aaaaaccgt cggacggta 
      actggggcg gtcacaacg tcttgcacc aaatgcgac 
      cgttcgttg ggtcgcacg aagaagagg cgcttttga 
      acgagatgg aagtgacag gacaagcgt ttgaagaca 
      tgcaggagc aaaactcac gcttgatcc aacagttgc 
      gtgagaagg acgacgcaa acttcaagt taatgtcag 
      agaggatca aatcaaacc agctccata aactggcaa 
      gggaggaga aggacgtgt tgaaggagc aagtcagca 
      ctttgacga ctcaagtcg aggctgcca atttggttg 
      tgcggaagt tggaggaga aggagcgga ttctccaga 
      acactctag ccacggttg agaaggagc tggcgttga 
      ggcagcaag cgatggaga tgcacaaga gaaaggcca 
      ttgaaagtg cgcaatcag cggccgatt tgaagctcc 
      atctcgaga aataccact cgcagatga aggaggcgc 
      agcaagtgg ttgccgaaa aaacaagct ctctggagg 
      cggaagcct acaagacca aacgactcc aagaggaga 
      tcgctcagt tgaagcgaa aggctgaaa ggatgaaga 
      agatggagc tggcgggaa ccacacttg atgaggtca 
      tgatggagg aaattcgcg aatataagg agacactga 
      cttgtccct cgtgcaaag tcaagagga aagatgcgg 
      ttttatcca aatgtttcc acgtttttt gctacgatt 
      gtctcaaga cgagatacg agacgaggc agaggaagt 
      gtccaaagt gcaactgcg cgtttggcg ccaacgact 
      atcatcgcc tgtttttgt cgaattaag gtacgttta 
      gagttaaga tttttccag cgctttgca gcagaacat 
      ttgtaataa aaagtcttg gtagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY