Status
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Gene information
General information
TC012098 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5937737..5942834
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012098
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06444

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (19 dpi)
    Days post injection

    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 10

    labial palp not fused - Number of animals showing the phenotype: 4

    antenna orientation irregular - Number of animals showing the phenotype: 3

    eyes size decreased - Number of animals showing the phenotype: 10

    ommatidia partially fused - Number of animals showing the phenotype: 10
    ommatidia number decreased - Number of animals showing the phenotype: 10
    elytra shortened - Number of animals showing the phenotype: 10

    Adult morphology
    eyes irregular - Number of animals showing the phenotype: 10


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012098 RA
      MSKRHAEES TSGGPPPIK KIHFEPHLI GPVSTLEEM 
DIKVLQFQN KKLAQRIEQ RYRTEQELR QRIEQLEKR 
QTQDDAMLN VVNRYWNQL NEDIRILLQ RFDAETADE 
SENKNESEA TTSFLMQLS TWDKEELND KLANRVQVS 
RRAVAKVIQ AFDRLMQRN EKITLALKG ELEGQDAPS 
VDEVICQTN VQLQAENRN LQALNTSLH EKYHSNSLK 
VAELQDSVT AKETEIAEL KNQIDDLQY ELEKVRNRN 
DKLETHLAE AIEKLKTYH QLHGDPEKG PQQKQIVQS 
NVSQAKLED LTKEVEELT ELANTRLAE LDKLHQTHR 
ETLKEVEKL KMDIRQLPE SVIVETTEY KCLQSQFSV 
LYNESMQLK TQLDEARQQ LQTSKNAHL RNIEMMESE 
ELIAQKKLR QEVMQLEDF LAQLRKEYE MLRIEFEQN 
LAANEQTGP INREMRHLI TSLQNNTQQ LKGEVHRYK 
RKYKEANAE IPKLKKEIE DLNAKLAQM QGASQDSKE 
NIKKEVKQE EDASSSESG AQVKEEPTS APAVKKEED 
ESEQTEEGD GDKASNSGN SPSAKKDAG VKQEKGAVK 
KEPTVKTEK DHREAQRAK DAKIAESEM VRDLKNQLK 
KALNEQKEM KLLLDMYKG VSKEQRDKV QLMAAEKKL 
RTELEDIRQ QIKKMQDSK RDDKRKLAE DEALKKIKQ 
LEEQKYELQ KQVASQKPS DGNWGGHNV LHQMRPFVG 
SHEEEALLN EMEVTGQAF EDMQEQNSR LIQQLREKD 
DANFKLMSE RIKSNQLHK LAREEKDVL KEQVSTLTT 
QVEAANLVV RKLEEKERI LQNTLATVE KELALRQQA 
MEMHKRKAI ESAQSAADL KLHLEKYHS QMKEAQQVV 
AEKTSSLEA EAYKTKRLQ EEIAQLKRK AERMKKMEL 
AGTTLDEVM MEEIREYKE TLTCPSCKV KRKDAVLSK 
CFHVFCYDC LKTRYETRQ RKCPKCNCA FGANDYHRL 
FLSN
    CDS sequence
    • >TC012098 RA
      atgtccaaa agacacgcc gaggagagc acgagcggg 
ggcccgcct cccatcaag aagatccac ttcgagccc 
catctcatc gggccggtg tccacgctc gaggagatg 
gacatcaag gtgttgcag ttccagaac aagaaactg 
gcccagaga attgagcaa cggtacagg acggagcaa 
gagttgcgg caaagaatc gagcagttg gagaagagg 
cagacacag gacgatgcc atgttgaac gttgttaat 
aggtattgg aaccagttg aatgaggat attaggatt 
ttgttgcaa cggtttgat gccgagacc gctgacgag 
tcggagaat aaaaatgag agtgaggcc acaacttcg 
tttttgatg caactttca acgtgggat aaggaggag 
ttgaacgat aagttggct aatagggtg caggtgtcg 
cggagggct gtggcgaag gtcatacaa gcctttgac 
cggctgatg caaaggaac gagaaaatt actttagcg 
cttaaaggc gagctagaa ggccaggat gcaccgagt 
gtggatgaa gtcatttgc caaaccaac gtccagctc 
caggctgag aaccgcaat ttgcaagcc ctcaataca 
tctctccat gaaaaatat cactcaaat agcctcaaa 
gttgccgaa ttgcaagac tcagtcacg gcgaaagaa 
acagaaatt gccgaatta aaaaaccaa attgacgat 
cttcaatac gagttggag aaagttcgc aatcgaaac 
gacaaactt gagacacat ttagctgaa gctatcgaa 
aaattgaaa acttatcac cagttgcat ggagatccg 
gaaaaggga ccccaacag aaacaaatc gttcagtct 
aatgtgtct caagccaaa cttgaggac ttgactaag 
gaagttgag gagttgacc gaattggca aacactcga 
ctagcagag ttggataag ttgcaccaa actcatcgc 
gagacgttg aaagaagtt gaaaagttg aaaatggat 
atacgacaa ctccccgaa agtgttatt gtggaaaca 
accgaatat aagtgtctc caatcacag ttttcggta 
ctttacaac gaatcaatg cagttgaaa acacagtta 
gacgaggcc agacaacag ctacaaacc agcaaaaat 
gcccatctg agaaacatt gaaatgatg gaaagtgag 
gaactcatc gctcagaag aagctcaga caggaggtt 
atgcaactt gaagacttc ctagctcaa ctgcgcaaa 
gaatacgaa atgttacga atcgaattt gagcagaat 
ctagccgcc aacgaacaa acaggtccc ataaatcgg 
gaaatgcga cacttgatc acatcactc caaaacaac 
acgcaacaa ttgaaaggg gaagtgcac cgctacaag 
agaaagtat aaggaagca aacgccgaa ataccgaaa 
ctaaagaaa gaaattgaa gatctgaac gctaaactt 
gcccaaatg cagggcgcc agccaggat agtaaagaa 
aacatcaag aaggaggtg aagcaggag gaagatgcg 
tcaagtagc gaatcgggg gcgcaggtc aaggaggaa 
ccgacttct gcaccggcg gtgaagaag gaggaagac 
gagtcagag caaactgaa gaaggagat ggggacaaa 
gcctcgaat tcgggcaac tcaccctct gctaaaaaa 
gacgcgggg gttaagcag gaaaagggc gctgtgaag 
aaggaacca actgttaag actgaaaag gaccatcgt 
gaggcacaa agggccaaa gacgcgaaa attgccgaa 
agtgaaatg gtgcgcgat cttaaaaat caactgaag 
aaagcactg aacgagcaa aaagagatg aaactcctc 
ctcgatatg tacaaaggt gtcagcaaa gaacaacgc 
gacaaagtc caactaatg gcagccgaa aaaaagctt 
cgtactgaa ttagaagat atccggcaa caaataaaa 
aaaatgcaa gacagtaaa agagacgac aagagaaaa 
ctcgccgag gacgaagcc ctcaaaaaa atcaaacaa 
ctggaggag caaaaatac gagttgcaa aagcaagta 
gcgtcgcaa aaaccgtcg gacggtaac tggggcggt 
cacaacgtc ttgcaccaa atgcgaccg ttcgttggg 
tcgcacgaa gaagaggcg cttttgaac gagatggaa 
gtgacagga caagcgttt gaagacatg caggagcaa 
aactcacgc ttgatccaa cagttgcgt gagaaggac 
gacgcaaac ttcaagtta atgtcagag aggatcaaa 
tcaaaccag ctccataaa ctggcaagg gaggagaag 
gacgtgttg aaggagcaa gtcagcact ttgacgact 
caagtcgag gctgccaat ttggttgtg cggaagttg 
gaggagaag gagcggatt ctccagaac actctagcc 
acggttgag aaggagctg gcgttgagg cagcaagcg 
atggagatg cacaagaga aaggccatt gaaagtgcg 
caatcagcg gccgatttg aagctccat ctcgagaaa 
taccactcg cagatgaag gaggcgcag caagtggtt 
gccgaaaaa acaagctct ctggaggcg gaagcctac 
aagaccaaa cgactccaa gaggagatc gctcagttg 
aagcgaaag gctgaaagg atgaagaag atggagctg 
gcgggaacc acacttgat gaggtcatg atggaggaa 
attcgcgaa tataaggag acactgact tgtccctcg 
tgcaaagtc aagaggaaa gatgcggtt ttatccaaa 
tgtttccac gttttttgc tacgattgt ctcaagacg 
agatacgag acgaggcag aggaagtgt ccaaagtgc 
aactgcgcg tttggcgcc aacgactat catcgcctg 
tttttgtcg aattaa
    mRNA sequence
    • >TC012098 RA
      gggcttcaa agggcgaaa ttaagccaa aatttgcaa 
attaaatgc aggaaaata gacaaacat ggcgcatgc 
gcagtgctg ccaacaggt tacggttgt ttgccctga 
gatcgataa ccaaaaacg agccaaaat taattaatt 
ttttaatta ataaatgtg attaaagtg tgtggtttg 
ttttagata aagtgtccc gtctttaat ttggggaaa 
aaatcgcaa ctttgagaa ataaccttg attcttatt 
gcaaatttc aatcagtgt ttgtttcta accccctag 
tcgtgtttt tggtataaa agacgtgct accgcgatt 
gtaacccca aaatgtcca aaagacacg ccgaggaga 
gcacgagcg ggggcccgc ctcccatca agaagatcc 
acttcgagc cccatctca tcgggccgg tgtccacgc 
tcgaggaga tggacatca aggtgttgc agttccaga 
acaagaaac tggcccaga gaattgagc aacggtaca 
ggacggagc aagagttgc ggcaaagaa tcgagcagt 
tggagaaga ggcagacac aggacgatg ccatgttga 
acgttgtta ataggtatt ggaaccagt tgaatgagg 
atattagga ttttgttgc aacggtttg atgccgaga 
ccgctgacg agtcggaga ataaaaatg agagtgagg 
ccacaactt cgtttttga tgcaacttt caacgtggg 
ataaggagg agttgaacg ataagttgg ctaataggg 
tgcaggtgt cgcggaggg ctgtggcga aggtcatac 
aagcctttg accggctga tgcaaagga acgagaaaa 
ttactttag cgcttaaag gcgagctag aaggccagg 
atgcaccga gtgtggatg aagtcattt gccaaacca 
acgtccagc tccaggctg agaaccgca atttgcaag 
ccctcaata catctctcc atgaaaaat atcactcaa 
atagcctca aagttgccg aattgcaag actcagtca 
cggcgaaag aaacagaaa ttgccgaat taaaaaacc 
aaattgacg atcttcaat acgagttgg agaaagttc 
gcaatcgaa acgacaaac ttgagacac atttagctg 
aagctatcg aaaaattga aaacttatc accagttgc 
atggagatc cggaaaagg gaccccaac agaaacaaa 
tcgttcagt ctaatgtgt ctcaagcca aacttgagg 
acttgacta aggaagttg aggagttga ccgaattgg 
caaacactc gactagcag agttggata agttgcacc 
aaactcatc gcgagacgt tgaaagaag ttgaaaagt 
tgaaaatgg atatacgac aactccccg aaagtgtta 
ttgtggaaa caaccgaat ataagtgtc tccaatcac 
agttttcgg tactttaca acgaatcaa tgcagttga 
aaacacagt tagacgagg ccagacaac agctacaaa 
ccagcaaaa atgcccatc tgagaaaca ttgaaatga 
tggaaagtg aggaactca tcgctcaga agaagctca 
gacaggagg ttatgcaac ttgaagact tcctagctc 
aactgcgca aagaatacg aaatgttac gaatcgaat 
ttgagcaga atctagccg ccaacgaac aaacaggtc 
ccataaatc gggaaatgc gacacttga tcacatcac 
tccaaaaca acacgcaac aattgaaag gggaagtgc 
accgctaca agagaaagt ataaggaag caaacgccg 
aaataccga aactaaaga aagaaattg aagatctga 
acgctaaac ttgcccaaa tgcagggcg ccagccagg 
atagtaaag aaaacatca agaaggagg tgaagcagg 
aggaagatg cgtcaagta gcgaatcgg gggcgcagg 
tcaaggagg aaccgactt ctgcaccgg cggtgaaga 
aggaggaag acgagtcag agcaaactg aagaaggag 
atggggaca aagcctcga attcgggca actcaccct 
ctgctaaaa aagacgcgg gggttaagc aggaaaagg 
gcgctgtga agaaggaac caactgtta agactgaaa 
aggaccatc gtgaggcac aaagggcca aagacgcga 
aaattgccg aaagtgaaa tggtgcgcg atcttaaaa 
atcaactga agaaagcac tgaacgagc aaaaagaga 
tgaaactcc tcctcgata tgtacaaag gtgtcagca 
aagaacaac gcgacaaag tccaactaa tggcagccg 
aaaaaaagc ttcgtactg aattagaag atatccggc 
aacaaataa aaaaaatgc aagacagta aaagagacg 
acaagagaa aactcgccg aggacgaag ccctcaaaa 
aaatcaaac aactggagg agcaaaaat acgagttgc 
aaaagcaag tagcgtcgc aaaaaccgt cggacggta 
actggggcg gtcacaacg tcttgcacc aaatgcgac 
cgttcgttg ggtcgcacg aagaagagg cgcttttga 
acgagatgg aagtgacag gacaagcgt ttgaagaca 
tgcaggagc aaaactcac gcttgatcc aacagttgc 
gtgagaagg acgacgcaa acttcaagt taatgtcag 
agaggatca aatcaaacc agctccata aactggcaa 
gggaggaga aggacgtgt tgaaggagc aagtcagca 
ctttgacga ctcaagtcg aggctgcca atttggttg 
tgcggaagt tggaggaga aggagcgga ttctccaga 
acactctag ccacggttg agaaggagc tggcgttga 
ggcagcaag cgatggaga tgcacaaga gaaaggcca 
ttgaaagtg cgcaatcag cggccgatt tgaagctcc 
atctcgaga aataccact cgcagatga aggaggcgc 
agcaagtgg ttgccgaaa aaacaagct ctctggagg 
cggaagcct acaagacca aacgactcc aagaggaga 
tcgctcagt tgaagcgaa aggctgaaa ggatgaaga 
agatggagc tggcgggaa ccacacttg atgaggtca 
tgatggagg aaattcgcg aatataagg agacactga 
cttgtccct cgtgcaaag tcaagagga aagatgcgg 
ttttatcca aatgtttcc acgtttttt gctacgatt 
gtctcaaga cgagatacg agacgaggc agaggaagt 
gtccaaagt gcaactgcg cgtttggcg ccaacgact 
atcatcgcc tgtttttgt cgaattaag gtacgttta 
gagttaaga tttttccag cgctttgca gcagaacat 
ttgtaataa aaagtcttg gtagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY