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Gene information
General information
TC012106 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5750348..5751455
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012106
    iBeetle screen iB_06443

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012106 RA
      MEKTELSDD ITHTFALSD QHYMVQVEE 
      MDDETEQLT AENTDDSNH DPIDKVGAP 
      LREQDRFLP IANVAKIMK KAIPETGKI 
      AKDARECVQ ECVSEFISF ITSEASDRC 
      YMEKRKTIN GEDILYAMS SLGFDNYVE 
      PLKLYLLKY REAAKSDKN LQPSEMAFD 
      ETSDESYNS TVTRNVIAA EPGGTETVI 
      YTYQDTNIH QFQIN
    CDS sequence
    • >TC012106 RA
      atggaaaag acagaactg tcagatgac atcacacac 
      acatttgcg ctctcagac cagcactac atggtccaa 
      gtggaagaa atggatgac gaaacggag cagctcacg 
      gccgaaaac acggacgac tcaaaccac gaccccatt 
      gacaaagtc ggggcgccg ctccgcgag caagacagg 
      tttttgccg atcgcaaac gtcgcaaag atcatgaag 
      aaagcaatt ccagaaacg ggaaagatc gcgaaggac 
      gcccgcgag tgtgtgcag gaatgcgtg tctgagttc 
      atcagtttt atcaccagc gaggccagt gatcggtgt 
      tatatggag aaacggaag actatcaac ggggaggat 
      attttatac gcaatgagt tcgttaggg tttgacaat 
      tatgtcgag cctttgaag ctgtacctc ctcaaatac 
      cgagaggcc gctaagagc gataagaat ctccaaccg 
      agcgaaatg gcctttgac gaaacctct gacgagagt 
      tacaactca acagtcaca cgaaacgtt atagctgcg 
      gaaccaggg ggcactgaa acggttatt tacacatat 
      caggacact aacattcac caattccaa attaactaa
    mRNA sequence
    • >TC012106 RA
      gaaagagca aaagcccac agccccacg aacgctgtt 
      tacgttcca aaatcgatc aaattatat tttttgtca 
      aaagtgcgt gtttttgtg agccactat ttcggtcca 
      attgagtgt aaacgttgc cctcgctga catggaaaa 
      gacagaact gtcagatga catcacaca cacatttgc 
      gctctcaga ccagcacta catggtcca agtggaaga 
      aatggatga cgaaacgga gcagctcac ggccgaaaa 
      cacggacga ctcaaacca cgaccccat tgacaaagt 
      cggggcgcc gctccgcga gcaagacag gtttttgcc 
      gatcgcaaa cgtcgcaaa gatcatgaa gaaagcaat 
      tccagaaac gggaaagat cgcgaagga cgcccgcga 
      gtgtgtgca ggaatgcgt gtctgagtt catcagttt 
      tatcaccag cgaggccag tgatcggtg ttatatgga 
      gaaacggaa gactatcaa cggggagga tattttata 
      cgcaatgag ttcgttagg gtttgacaa ttatgtcga 
      gcctttgaa gctgtacct cctcaaata ccgagaggc 
      cgctaagag cgataagaa tctccaacc gagcgaaat 
      ggcctttga cgaaacctc tgacgagag ttacaactc 
      aacagtcac acgaaacgt tatagctgc ggaaccagg 
      gggcactga aacggttat ttacacata tcaggacac 
      taacattca ccaattcca aattaacta agctgtaaa 
      tttcgttat aatttattt ttttctgta tatagtgat 
      aagttgcat atttttatt aataaacgg tattttaag 
      aaggggttt tacttactc aaacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY