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Gene information
General information
TC005161 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451654.1: 241..6873
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005161
    iBeetle screen iB_06434

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005161 RA
      MTTHSGKLC KCPKSGCIF AARSMGELK QHFRTHSDV 
      KSFSCSICD YRGKTKQQL KHHLTVHSG TKKYACTKC 
      SFSARTMSH LKRHIRLHT GSKPYSCPH CAYKCNTLE 
      NLRKHVLLT NKHRGKCIY ECKFCAESP FQSNFAKEF 
      KVHLVTEHS AMFKSGREA ATFVAGIYD EQNDCTEFN 
      PASSEDDES KPTSAKDRS APPKVIILQ HLKSEVDKD 
      PVVASSSKD TGEEGNPDE MLPMFIVSR DETVSVDNL 
      TETWNVVGS YDVEESGAL VPFNSDNVL FQGHF
    CDS sequence
    • >TC005161 RA
      atgaccaca cattcgggc aaattatgc aaatgcccg 
      aaatcgggc tgtattttc gccgcccgc agcatgggc 
      gagttgaag cagcatttc cggacgcat tccgacgtg 
      aaaagcttc tcttgcagc atttgcgac taccgcgga 
      aagaccaaa cagcagtta aaacaccac ttgaccgtc 
      cacagtggg acgaagaag tatgcttgc accaagtgc 
      tcgttctcg gcgaggacg atgtctcat ttgaagaga 
      catatccgc ctacacacc ggctccaag ccctacagt 
      tgcccccac tgcgcctac aaatgcaac actttggaa 
      aatctgaga aaacatgta ctgttgacc aataaacac 
      cgagggaag tgtatttac gaatgcaag ttctgtgca 
      gagtcgccc ttccaaagc aactttgcc aaagaattt 
      aaggttcat cttgttacc gagcattca gcaatgttt 
      aaaagtgga cgggaagct gctaccttt gttgcagga 
      atttatgac gagcagaac gattgcacc gaatttaat 
      cctgcaagc tccgaggac gacgaatcg aagcctaca 
      agtgcaaaa gaccggtcg gctccgccg aaggtgata 
      atcctgcag cacttgaag agcgaggtg gacaaagat 
      cccgtggtg gcttcgagc agtaaagac acgggtgaa 
      gaaggcaat cctgacgag atgttgccg atgtttatt 
      gtgtctcgc gatgagaca gtttcagta gataatctc 
      acggagact tggaatgtg gtcggtagc tacgacgtt 
      gaagagtcc ggagcgtta gttcctttc aatagcgat 
      aacgttctc ttccaggga catttctga
    mRNA sequence
    • >TC005161 RA
      ctaatagtt tccttcgac gtaacgata cagcgaaac 
      tttggccat tcattatcg atcctgcaa cagcacatg 
      accacacat tcgggcaaa ttatgcaaa tgcccgaaa 
      tcgggctgt attttcgcc gcccgcagc atgggcgag 
      ttgaagcag catttccgg acgcattcc gacgtgaaa 
      agcttctct tgcagcatt tgcgactac cgcggaaag 
      accaaacag cagttaaaa caccacttg accgtccac 
      agtgggacg aagaagtat gcttgcacc aagtgctcg 
      ttctcggcg aggacgatg tctcatttg aagagacat 
      atccgccta cacaccggc tccaagccc tacagttgc 
      ccccactgc gcctacaaa tgcaacact ttggaaaat 
      ctgagaaaa catgtactg ttgaccaat aaacaccga 
      gggaagtgt atttacgaa tgcaagttc tgtgcagag 
      tcgcccttc caaagcaac tttgccaaa gaatttaag 
      gttcatctt gttaccgag cattcagca atgtttaaa 
      agtggacgg gaagctgct acctttgtt gcaggaatt 
      tatgacgag cagaacgat tgcaccgaa tttaatcct 
      gcaagctcc gaggacgac gaatcgaag cctacaagt 
      gcaaaagac cggtcggct ccgccgaag gtgataatc 
      ctgcagcac ttgaagagc gaggtggac aaagatccc 
      gtggtggct tcgagcagt aaagacacg ggtgaagaa 
      ggcaatcct gacgagatg ttgccgatg tttattgtg 
      tctcgcgat gagacagtt tcagtagat aatctcacg 
      gagacttgg aatgtggtc ggtagctac gacgttgaa 
      gagtccgga gcgttagtt cctttcaat agcgataac 
      gttctcttc cagggacat ttctgacaa ttttaataa 
      gtaacctag gtgtgtaat ttaaataaa gccgtataa 
      aaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY