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Gene information
General information
TC034280
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010994
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3433920..3435000
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034280
    iBeetle screen iB_06430

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034280 RA
      MSDYDESFD EDDDDCGIL KQALHVEFP 
      ENFNPNKVP QTGEEYLQH VIYERRQCK 
      TWLTADIDR SKFVNQQTL KLEVENPIK 
      GASSLLPSK KWQTQKIDE FTSFQKFIW 
      FKIEKESTV SSFNEEHFL EKVQSADPQ 
      FSELTAYTQ AAKIRMFQI ISKHISSIP 
      KGESIGKKT GAWIYAILT LLEKPLSPD 
      SCYTLREFA KRCLAVRAN LDEPASEEL 
      YKPLNLFIC IIARFYNQL DLADPF
    CDS sequence
    • >TC034280 RA
      atgagtgat tatgatgaa agttttgac gaggatgac 
      gatgattgt ggtattttg aaacaagca cttcatgtg 
      gagtttcca gaaaacttt aatcccaat aaagtgccc 
      caaaccggt gaagaatat ctacagcac gttatttac 
      gaaagacga cagtgtaaa acttggctc actgcagat 
      attgacaga agcaaattt gtaaatcaa caaactttg 
      aaactggaa gtggaaaac ccaattaaa ggagcttcc 
      tcattgctt ccatccaaa aaatggcaa acacaaaaa 
      attgatgaa tttacaagt ttccaaaaa ttcatttgg 
      tttaaaatc gaaaaagaa tcaacggtg tcatcattt 
      aacgaagaa cattttttg gagaaagtt caatcagcg 
      gatcctcaa ttttcggag cttacagcc tacactcaa 
      gccgcaaaa ataaggatg tttcaaata atttcaaaa 
      catataagt tcaataccc aagggggag agtatagga 
      aagaaaact ggagcttgg atttatgca attttgaca 
      ctcttagaa aaaccctta agccctgat tcatgctac 
      actttgcgt gagttcgcc aaaaggtgt ttagctgta 
      agagccaac ttggatgaa cctgcaagt gaggagttg 
      tataagccg ttaaattta ttcatttgt ataatagcc 
      aggttttat aatcaacta gatttagct gaccctttc 
      tga
    mRNA sequence
    • >TC034280 RA
      caagtaacg taaccttac ttttcgtat caaaaccga 
      agacgattt aacagcaaa tattgcaaa aatgagtga 
      ttatgatga aagttttga cgaggatga cgatgattg 
      tggtatttt gaaacaagc acttcatgt ggagtttcc 
      agaaaactt taatcccaa taaagtgcc ccaaaccgg 
      tgaagaata tctacagca cgttattta cgaaagacg 
      acagtgtaa aacttggct cactgcaga tattgacag 
      aagcaaatt tgtaaatca acaaacttt gaaactgga 
      agtggaaaa cccaattaa aggagcttc ctcattgct 
      tccatccaa aaaatggca aacacaaaa aattgatga 
      atttacaag tttccaaaa attcatttg gtttaaaat 
      cgaaaaaga atcaacggt gtcatcatt taacgaaga 
      acatttttt ggagaaagt tcaatcagc ggatcctca 
      attttcgga gcttacagc ctacactca agccgcaaa 
      aataaggat gtttcaaat aatttcaaa acatataag 
      ttcaatacc caaggggga gagtatagg aaagaaaac 
      tggagcttg gatttatgc aattttgac actcttaga 
      aaaaccctt aagccctga ttcatgcta cactttgcg 
      tgagttcgc caaaaggtg tttagctgt aagagccaa 
      cttggatga acctgcaag tgaggagtt gtataagcc 
      gttaaattt attcatttg tataatagc caggtttta 
      taatcaact agatttagc tgacccttt ctgattaaa 
      aacaaaaaa aaaatgttt aagagtttt attctaaaa 
      gccaatctt atatcctaa aaagttgtt aaactcgct 
      attcactac ttgatggcg ccagcaata gccaccttt 
      gcgactctt agctgtttc aaaactatg aacacgcca 
      acgtcgcaa aattagctg taataaatt attcattca 
      tac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY