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Gene information
General information
TC031230
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010975,TC010976 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3035979..3052511
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031230
    iBeetle screen iB_06429

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031230 RA
      MEGGESPLE VLSRAATMV QENQNALAS 
AEESSKQSS NKQLPTAKW KRDRRSRLP 
EYSRKSDPK IAETFGTEP PETPNQQAA 
NGQDNAPLD MSVRQRGLP PSYSQTINN 
PGYRSNYRP SVIQKNRED LPPGISMCD 
PIIDEHFRR SLGNDYLSL FSNNNVSRE 
AIPVKPPPK VPTTTNVIE LMDTAGLSV 
DDHFAKALG DTWVKLNKK EELKNSTSD 
NHSIVTI
    CDS sequence
    • >TC031230 RA
      atggagggc ggcgagtct ccgctggag gtgctgtcg 
agggccgct accatggtg caagaaaac cagaacgct 
ctggcctct gcagaggag tcgtcgaag cagtcgagc 
aacaagcaa cttccgacc gccaaatgg aagagggat 
cgacgttcg aggcttccg gagtactcg cggaagtcc 
gatccgaaa atcgccgag acgttcgga acggaaccg 
ccggaaacg ccaaatcaa caggccgcg aacggtcag 
gataatgcc ccgctcgat atgagtgtt agacaaaga 
gggttgcct ccttcctac tcgcaaact attaataat 
ccaggatac agatctaac tacagaccc agtgttata 
cagaaaaat agagaagat cttcctcct ggaatatcg 
atgtgcgat ccaatcatc gatgagcat ttccgtcgt 
tctctaggc aacgactat ctttctctc ttttcaaac 
aacaatgtt tctagagag gcaatccct gttaaacct 
cctccgaaa gttcccacc acaacgaac gtgattgaa 
ttaatggac actgcaggt ttatctgtg gacgatcac 
ttcgccaaa gctctaggt gacacatgg gtgaaacta 
aataaaaaa gaagaactc aaaaacagt accagtgac 
aatcacagt atagttact atttaa
    mRNA sequence
    • >TC031230 RA
      caggtttga ccgaaatgt caaattctt tgcacggtt 
ttacttgtt agtgtgtaa tacagtgta aaaagggca 
taattctct tatttttag tgagattag ggttgtttt 
tacccaggc cacttgatc ttgaaggga ggacatgga 
gggcggcga gtctccgct ggaggtgct gtcgagggc 
cgctaccat ggtgcaaga aaaccagaa cgctctggc 
ctctgcaga ggagtcgtc gaagcagtc gagcaacaa 
gcaacttcc gaccgccaa atggaagag ggatcgacg 
ttcgaggct tccggagta ctcgcggaa gtccgatcc 
gaaaatcgc cgagacgtt cggaacgga accgccgga 
aacgccaaa tcaacaggc cgcgaacgg tcaggataa 
tgccccgct cgatatgag tgttagaca aagagggtt 
gcctccttc ctactcgca aactattaa taatccagg 
atacagatc taactacag acccagtgt tatacagaa 
aaatagaga agatcttcc tcctggaat atcgatgtg 
cgatccaat catcgatga gcatttccg tcgttctct 
aggcaacga ctatctttc tctcttttc aaacaacaa 
tgtttctag agaggcaat ccctgttaa acctcctcc 
gaaagttcc caccacaac gaacgtgat tgaattaat 
ggacactgc aggtttatc tgtggacga tcacttcgc 
caaagctct aggtgacac atgggtgaa actaaataa 
aaaagaaga actcaaaaa cagtaccag tgacaatca 
cagtatagt tactattta aaaaagctg aatcgatgt 
gtgtttaac gtttaaatg tatggtgtt aaatgtaag 
tttatcgaa ataacgtac aaatgtatc ttaggctta 
gataggttt caccgaagg tcgcgaatt gaggtttgt 
ttcattcag ggatcgaat gacttgttt tcttatttt 
tatgtgtag tttctattg agagcacaa cctgacaaa 
ggctgcctt ccaattaac cagttaatg attccttat 
gttccattg ttgtaaata ttggactgt cttgtatat 
aggtttttg taaatacaa aagtgttat cgcctagtt 
tagcacttc atatttata attaattta gtccactct 
tgttgctta gttctaaac tccattatg ttactttgg 
tatacgtaa ttaaaatga caaatctaa tacaaactt 
ttcttacca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY