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Gene information
General information
TC001916 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 1624726..1732489
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001916
    iBeetle screen iB_06426

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001916 RA
      MPQPLTAPP QGEVKVGRG YIEDSNLYC RCLTKVVLS 
      FCFFLCVCV CHRKMAPLW SVLLSFLLF IIAYTWLRR 
      SRNLPPGPW NLPVIGYLA WLNPQFPYL TLTSLSKKY 
      GPIYGLYLG SIYTVVISD AKLIKKTFN KDASSGRAP 
      LHLTHGIMK GFGLICAQG DLWKDQRKF VHNTLRTLG 
      ASKVSPNRP TMEALILHH VSDLVQHIK SLGESVTLD 
      PLDSLRHSL GSAINQMVF GKCWSRDDA TWKWLQHLQ 
      EEGTKHIGV AGPLNFLPL LRFLPKSKQ TINFLTEGI 
      RDTHQIYRE IIEEQKNSS NETVSNVIQ AFLAEKEKR 
      KNEDSVKFY NDQQFHYLL ADIFGASLD TTLTTLRWY 
      VLYMAVHQD VQKKVRSLL NDLTLEQIA MVPYFEATI 
      AEVQRIRPV VPVGIPHGS VEELEIAQY KVPPGTMIV 
      PLQWAVHMD ANIWDEPEV FKPERFINE EGKFFKPEA 
      FIPFQAGKR MCVGDELAR MFLYLFGAA LVKNFAISC 
      MGEVDLTGD CGITLTPKP HELIFTSL
    CDS sequence
    • >TC001916 RA
      atgccacaa cctctaacc gcaccacca caaggcgaa 
      gtgaaagtg gggagaggc tatatcgaa gactccaac 
      ctgtactgc aggtgtctt accaaagtc gtcttaagt 
      ttttgtttt tttttgtgc gtgtgtgtt tgtcaccga 
      aagatggct ccgctgtgg tcagtgctc ttatcgttt 
      ctcctgttc atcatcgcg tacacctgg ctccgtcgc 
      tctcgtaac ttgcctcca ggtccttgg aacctacct 
      gtgatcggt tatttggcc tggttgaac cctcagttt 
      ccttacctg actctaact tctctctct aaaaagtac 
      ggtcccatc tacggcctc tatctgggc agtatctac 
      accgtggtg atctccgac gccaaactc atcaagaaa 
      accttcaac aaagacgca tcctcaggc cgtgcgccc 
      ttgcacctg acccatggc ataatgaag ggtttcggc 
      ctgatttgc gcacaaggc gacttatgg aaagatcaa 
      cgcaaattc gtccacaac accttacga actcttggt 
      gcttccaaa gtgagtcca aaccgaccc accatggaa 
      gccctaatc ctgcaccac gtctcagac cttgtccaa 
      cacattaaa agcctcggt gaatccgtc actctggac 
      cctcttgac tcgctccgc catagcctt ggctcggcc 
      atcaaccaa atggtgttc ggtaagtgt tggtcacga 
      gacgatgcc acttggaag tggctgcaa catcttcag 
      gaagagggg acgaaacac attggggtt gccgggcct 
      ttgaatttt ttgccgctt ttaaggttt ttgccaaag 
      tcaaaacaa acaataaat ttcttaacc gagggaatc 
      cgagacacc caccaaata tatcgcgaa ataatcgaa 
      gagcaaaaa aattcgtct aacgaaact gttagtaat 
      gtcatccaa gcgtttctt gctgagaaa gagaaacga 
      aaaaatgaa gacagtgtt aagttctac aacgaccaa 
      caatttcat tacttgttg gctgatatt ttcggggcc 
      agtttggac acgactttg accacgtta cggtggtac 
      gtgctgtac atggcagtg caccaagac gtccagaaa 
      aaagtgcga tcactttta aatgatcta acacttgaa 
      caaatcgca atggtgcca tattttgaa gcaactatt 
      gctgaagtt cagagaatt cgaccggtc gtacctgtg 
      ggtattcca cacggaagt gtggaagag ctggaaatt 
      gctcagtat aaggttcca ccaggtacc atgatagta 
      ccattacaa tgggcagtt catatggat gcgaatatt 
      tgggacgaa cctgaagtg ttcaaacct gaacggttt 
      atcaacgaa gaagggaag tttttcaaa ccggaagct 
      tttattccg tttcaagcg ggtaaaaga atgtgtgtc 
      ggtgatgaa ttggctcga atgttcctc tacctcttt 
      ggtgcagct ttggtaaaa aatttcgcc atcagttgt 
      atgggagaa gttgatttg actggagat tgtggcatt 
      acactaact ccgaaacct cacgaattg atttttaca 
      agtttgtaa
    mRNA sequence
    • >TC001916 RA
      ataaaagaa atatggcgt cacactcaa cgcgaaata 
      tttattaac tttgaaaat tcttaatta atattacaa 
      aaaattatt gattcatta aactaaact cacctgaaa 
      attgattaa acgattttg gtctgcacc ggttggcaa 
      tatttgcac tttatgcat ttttttgtt ttacagtac 
      agacgccac gccaacttg actatgcca caacctcta 
      accgcacca ccacaaggc gaagtgaaa gtggggaga 
      ggctatatc gaagactcc aacctgtac tgcaggtgt 
      cttaccaaa gtcgtctta agtttttgt ttttttttg 
      tgcgtgtgt gtttgtcac cgaaagatg gctccgctg 
      tggtcagtg ctcttatcg tttctcctg ttcatcatc 
      gcgtacacc tggctccgt cgctctcgt aacttgcct 
      ccaggtcct tggaaccta cctgtgatc ggttatttg 
      gcctggttg aaccctcag tttccttac ctgactcta 
      acttctctc tctaaaaag tacggtccc atctacggc 
      ctctatctg ggcagtatc tacaccgtg gtgatctcc 
      gacgccaaa ctcatcaag aaaaccttc aacaaagac 
      gcatcctca ggccgtgcg cccttgcac ctgacccat 
      ggcataatg aagggtttc ggcctgatt tgcgcacaa 
      ggcgactta tggaaagat caacgcaaa ttcgtccac 
      aacacctta cgaactctt ggtgcttcc aaagtgagt 
      ccaaaccga cccaccatg gaagcccta atcctgcac 
      cacgtctca gaccttgtc caacacatt aaaagcctc 
      ggtgaatcc gtcactctg gaccctctt gactcgctc 
      cgccatagc cttggctcg gccatcaac caaatggtg 
      ttcggtaag tgttggtca cgagacgat gccacttgg 
      aagtggctg caacatctt caggaagag gggacgaaa 
      cacattggg gttgccggg cctttgaat tttttgccg 
      cttttaagg tttttgcca aagtcaaaa caaacaata 
      aatttctta accgaggga atccgagac acccaccaa 
      atatatcgc gaaataatc gaagagcaa aaaaattcg 
      tctaacgaa actgttagt aatgtcatc caagcgttt 
      cttgctgag aaagagaaa cgaaaaaat gaagacagt 
      gttaagttc tacaacgac caacaattt cattacttg 
      ttggctgat attttcggg gccagtttg gacacgact 
      ttgaccacg ttacggtgg tacgtgctg tacatggca 
      gtgcaccaa gacgtccag aaaaaagtg cgatcactt 
      ttaaatgat ctaacactt gaacaaatc gcaatggtg 
      ccatatttt gaagcaact attgctgaa gttcagaga 
      attcgaccg gtcgtacct gtgggtatt ccacacgga 
      agtgtggaa gagctggaa attgctcag tataaggtt 
      ccaccaggt accatgata gtaccatta caatgggca 
      gttcatatg gatgcgaat atttgggac gaacctgaa 
      gtgttcaaa cctgaacgg tttatcaac gaagaaggg 
      aagtttttc aaaccggaa gcttttatt ccgtttcaa 
      gcgggtaaa agaatgtgt gtcggtgat gaattggct 
      cgaatgttc ctctacctc tttggtgca gctttggta 
      aaaaatttc gccatcagt tgtatggga gaagttgat 
      ttgactgga gattgtggc attacacta actccgaaa 
      cctcacgaa ttgattttt acaagtttg taattcttt 
      tgtattaga aggaaagga taaaataaa gtcagtttt 
      ctatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY