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Gene information
General information
TC012308 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3984422..3988161
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012308
    iBeetle screen iB_06416

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012308 RA
      MPKGKRKGK SDRGRFDGS LHTSDDDSY NDNNSVISS 
      YSENKSLED GEDIEEFAH EQFEDKLIE MLDGLSQKS 
      SQGRINCIQ SLRKALIKK YVPNFVKER HFTICDSIE 
      KSLKKGGGL EKAAAAELA PLISVQLGE EDAGEIVDK 
      TLRPVLMTV ACDGSVGAA ARAKCCLAL GMMGFLSGR 
      QLGDTLSLM QNLETIFSA SYLKGDGSI PNVTAEVAN 
      LHAAALSAW TLLLTNMPL GSLTDLMDS KALPSLDQL 
      SELLESPHL EVRLTAGEA LALIFEEGR KDSADFADD 
      FAVYITDVI KKLATDSHK YRAKKDRKQ QRATFRDIL 
      SYIEDDVVP DVTVKFGRE VLMLDTWAR RKQYDALCT 
      VLGPGINIH LGENDLLRD IFEITAPIV QPHDTPAHA 
      QQHMERCRK RLLNAAAFK ARTISRAKN RDKRSDF
    CDS sequence
    • >TC012308 RA
      atgccgaaa gggaaacga aaaggaaag tctgatcgt 
      ggtcgtttc gatggctct ttgcacacc tcggacgac 
      gattcgtac aatgacaac aacagcgtc atcagcagc 
      tactcggag aataagagt ctcgaggac ggcgaggac 
      atcgaggag ttcgctcac gagcagttc gaggataaa 
      ctgatcgag atgttggac ggtttgagt cagaagtcg 
      tcgcagggg aggataaac tgtatccag tcgctccgt 
      aaagctctg attaagaaa tacgtccct aatttcgtc 
      aaggagaga cactttacg atctgtgac agtatcgag 
      aagagcttg aagaaaggg ggcggtttg gagaaggcg 
      gccgccgcc gagttggca cccttgatt agcgtgcaa 
      ctgggggag gaagatgcg ggcgagatt gtcgataag 
      accttgaga cccgttttg atgactgtg gcttgtgat 
      ggctcggtt ggggcggct gccagggcc aagtgttgc 
      ctcgctttg ggcatgatg ggtttcctg tcgggccgc 
      caactgggc gatactttg tctctcatg caaaatttg 
      gaaaccatt ttctcggcc agttatcta aaaggcgac 
      ggctccatt cctaacgta actgcggaa gttgcgaac 
      ttgcacgca gccgcgtta agtgcatgg accttattg 
      ctgaccaat atgccacta gggagtctc accgattta 
      atggacagc aaagcctta ccatcgcta gatcagtta 
      tctgaactg ttggaatcg ccgcaccta gaagtccgt 
      ttaacggca ggtgaagcc ctagcgctg atttttgaa 
      gaagggagg aaagacagt gcggatttt gctgacgac 
      tttgcagtc tatatcaca gatgtgatt aagaaactg 
      gccacggat tcgcataaa tatcgcgcg aagaaggac 
      cgcaaacaa cagagggcc acgttcagg gacattctt 
      tcatacatt gaagacgat gttgttccc gacgtgact 
      gtgaagttt ggtcgggaa gtcctgatg ttggacacg 
      tgggcgcgt aggaaacag tacgacgcc ttgtgtacc 
      gttttaggg cctggaatt aacatccat ttgggggag 
      aatgacctt ctgagggac atatttgag attacagcc 
      cccatagtg cagccacat gacacacca gcgcacgca 
      caacaacac atggagagg tgtcgcaag cgattactt 
      aatgccgca gcgtttaaa gcaaggacg atatcacgg 
      gcgaaaaat cgggacaag cgatcagac ttttaa
    mRNA sequence
    • >TC012308 RA
      tgggccagg caagggccc aagtccgct gtttgattt 
      tttatcgat tatttgggc ccaacgttg cgcaacctg 
      gcggcactg gggccagtt aaaaggcgc cttgtcgct 
      gtcatcgcg aagttgacc gtctcgtgt tcaactgga 
      aacatgtta ttgagtttt attccaatt ttatctcaa 
      attaaatgt caggtgtcg agtgcatgt gtaaaagat 
      tgcttaaca aacagttta ccgtcttga tctagtgcg 
      aaattagct gttttattt agtaaattt aagaaaaag 
      tttatttaa ttgtcgacc atgccgaaa gggaaacga 
      aaaggaaag tctgatcgt ggtcgtttc gatggctct 
      ttgcacacc tcggacgac gattcgtac aatgacaac 
      aacagcgtc atcagcagc tactcggag aataagagt 
      ctcgaggac ggcgaggac atcgaggag ttcgctcac 
      gagcagttc gaggataaa ctgatcgag atgttggac 
      ggtttgagt cagaagtcg tcgcagggg aggataaac 
      tgtatccag tcgctccgt aaagctctg attaagaaa 
      tacgtccct aatttcgtc aaggagaga cactttacg 
      atctgtgac agtatcgag aagagcttg aagaaaggg 
      ggcggtttg gagaaggcg gccgccgcc gagttggca 
      cccttgatt agcgtgcaa ctgggggag gaagatgcg 
      ggcgagatt gtcgataag accttgaga cccgttttg 
      atgactgtg gcttgtgat ggctcggtt ggggcggct 
      gccagggcc aagtgttgc ctcgctttg ggcatgatg 
      ggtttcctg tcgggccgc caactgggc gatactttg 
      tctctcatg caaaatttg gaaaccatt ttctcggcc 
      agttatcta aaaggcgac ggctccatt cctaacgta 
      actgcggaa gttgcgaac ttgcacgca gccgcgtta 
      agtgcatgg accttattg ctgaccaat atgccacta 
      gggagtctc accgattta atggacagc aaagcctta 
      ccatcgcta gatcagtta tctgaactg ttggaatcg 
      ccgcaccta gaagtccgt ttaacggca ggtgaagcc 
      ctagcgctg atttttgaa gaagggagg aaagacagt 
      gcggatttt gctgacgac tttgcagtc tatatcaca 
      gatgtgatt aagaaactg gccacggat tcgcataaa 
      tatcgcgcg aagaaggac cgcaaacaa cagagggcc 
      acgttcagg gacattctt tcatacatt gaagacgat 
      gttgttccc gacgtgact gtgaagttt ggtcgggaa 
      gtcctgatg ttggacacg tgggcgcgt aggaaacag 
      tacgacgcc ttgtgtacc gttttaggg cctggaatt 
      aacatccat ttgggggag aatgacctt ctgagggac 
      atatttgag attacagcc cccatagtg cagccacat 
      gacacacca gcgcacgca caacaacac atggagagg 
      tgtcgcaag cgattactt aatgccgca gcgtttaaa 
      gcaaggacg atatcacgg gcgaaaaat cgggacaag 
      cgatcagac ttttaatgc atattatgg gatatttat 
      gtatttatt ttagctcaa ttatatacc gacagtagc 
      cgattttgt ttttatctg tagcagtag cgagaattt 
      gttgtcatt aattatttg aaatttgta aatacgttt 
      attaataaa tttacaaat ttaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY