Status
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Gene information
General information
TC012279 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3717162..3736879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012279
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06412

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012279 RA
      MLFKVVKCP SDELSLTNC AVVNDADFS QSTEHIEVI 
      TGGVKKFIF SIKKDPRVH RNEVAFSVP QRKWATLSI 
      NDDIEVNPY KEEDYITSI VLEADYMQK KTTSQEQYD 
      TDEMAKEFI YSFPNQMFT VGQQLAFLF KDKKILSLL 
      VKDIEVVNI NTLKQGGEA KPKKGKLGK LTPNTVIQF 
      EKAETSALN LVGKAKGKI VRQSIINPD WDFQKMGIG 
      GLGKEFNAI FRRAFASRV FPPEIVEQL GCKHVKGIL 
      LYGPPGTGK TLMARQIGK MLNAREPKI VNGPQILDK 
      YVGESEANI RRLFAEAEE EEKRAGPNS GLHIIIFDE 
      IDAICKQRG SVAGNTGVH DTVVNQLLS KIDGVEQLN 
      NILVIGMTN RRDMIDEAL LRPGRLEVQ VEISLPNED 
      GRFEILNIH TSRMRQYKK ISSDVDTRE LATLTKNFS 
      GAELEGLVR AAQSTAMNR LIKASNKVE VDPEAISKL 
      LVTRGDFLN ALENDIKPA FGTALEILE QFLARGITV 
      WGSPVSSIL EDGRLFIQQ AKATDTSGL VSVLIEGPP 
      NAGKTALAA QLAKESDFP FVKVCSPEE MVGYTETAK 
      CLHIRKVFD DAYRSTLSC ILVDNIERL LDYGPIGPR 
      YSNLTLQAL LVLLKKQPP PGKKLLILC TTSRRQVLE 
      DMEMLSAFT AVLHLPNLS KADQLLTVL ENSEVFSQQ 
      ELKKISQQV HGKRIFIGI KKLLALIDM ARQTDEKFR 
      VMKLITKLE EDGALEDPG MA
    CDS sequence
    • >TC012279 RA
      atgttgttt aaagtggtg aaatgcccc tcagatgag 
      ctgtcattg acaaattgt gctgtcgtg aatgacgcc 
      gacttttca caatctaca gagcatatt gaagtcatc 
      acaggagga gtgaaaaag ttcattttt tccattaag 
      aaagacccg agagttcac aggaatgag gttgcgttc 
      agtgtacca cagagaaaa tgggccacc ttgtccatt 
      aatgatgac attgaagtt aatccttac aaggaagaa 
      gattacata acttcgatc gtcctagag gctgattac 
      atgcaaaag aaaacaaca tcgcaagag caatatgac 
      acagatgaa atggctaaa gaatttatt tactcgttt 
      ccaaatcag atgtttact gttggtcaa caactggct 
      ttcctcttt aaagacaaa aagatactt agcctgctc 
      gtcaaggac attgaagtt gtcaacatc aacaccctc 
      aagcaggga ggcgaggcc aagcccaaa aaaggtaaa 
      ctgggaaaa ttgacaccc aacactgtc atacagttt 
      gaaaaggcc gaaacttcg gcccttaat ttagtaggc 
      aaagccaaa ggcaaaatt gtgcgtcag tcgataatt 
      aaccctgat tgggacttc caaaaaatg ggaatcggc 
      ggtttgggc aaggaattt aacgctata ttcagaaga 
      gcttttgcg tcaagagtt ttcccgcca gagattgtg 
      gaacaactg ggctgcaaa cacgtcaaa ggtattctt 
      ctctatggc cctccagga actggtaaa actttgatg 
      gcgcgacaa attggaaaa atgttaaat gccagagag 
      ccgaaaata gtcaacggg ccccaaatt ctagataaa 
      tatgtgggc gagtctgag gctaatatc agaagattg 
      tttgcagaa gctgaagaa gaagaaaaa agagccggg 
      ccaaacagt ggtttgcac atcattatc tttgatgaa 
      atcgatgcg atttgtaag caaagagga tctgttgct 
      ggtaacaca ggtgttcac gatacggtc gtgaaccaa 
      ttgttgtca aaaatcgat ggagtggag caacttaac 
      aatattctt gtcataggg atgacaaac agacgcgat 
      atgattgat gaagctttg ctcagacct ggtagattg 
      gaagtacag gtcgagata agtttgcca aatgaggat 
      ggacgattc gagattttg aatattcat acgagcaga 
      atgaggcag tataagaaa attagttct gatgttgac 
      acaagggaa ctggcaacg ttaactaaa aacttctcc 
      ggcgccgaa cttgaaggt ttagtcaga gcagcccaa 
      tccacagcc atgaaccgc ttgatcaaa gcttccaat 
      aaagtcgaa gtagatcca gaagcaatt agtaaatta 
      ttagtaaca cgtggcgac tttttaaat gctttagaa 
      aacgacatc aaaccagct ttcggcaca gctttagaa 
      attttagaa cagttctta gcacgtgga attactgta 
      tggggcagc ccagtcagt agcattcta gaagatggt 
      cggttattt atccagcaa gctaaagcc accgacact 
      tcaggtttg gtgtccgtt ctgatcgag ggtccaccg 
      aacgctggc aaaaccgct ttagccgcg cagcttgcc 
      aaagaatcc gattttccc ttcgttaaa gtgtgctca 
      cctgaagaa atggtgggt tatactgaa actgccaaa 
      tgcttacat atcaggaaa gtgtttgat gatgcttac 
      cgctcaaca ctgagttgc attctagtc gataatatt 
      gaacgatta ttagattac ggaccgatt ggtccccga 
      tattccaat ttaacatta caggcactt ttagtattg 
      ttaaagaaa cagccaccg cctggaaaa aaattgttg 
      attttgtgt acaactagt cgaaggcaa gttttagaa 
      gatatggaa atgttatcc gccttcact gctgtttta 
      catttacca aatctttca aaagcggat cagttactc 
      acagttctt gagaattcc gaagttttt agtcaacaa 
      gaattgaag aagatcagc caacaagtg cacggcaaa 
      cgcattttc attgggata aagaaactc ctggctctg 
      attgacatg gcccgccag accgacgaa aagttccga 
      gtgatgaaa ttgatcaca aaattggag gaggacggt 
      gcactggaa gatcccggg atggcctaa
    mRNA sequence
    • >TC012279 RA
      cattgttgc aaaattact agttaacgt aaagtaggg 
      gtaacgagg gcagttgat tggcgacac tgtaaaacg 
      ggtaagtga ggttaagtt gtgttaggt taaggttgt 
      tatcctgtc agtgctgtg taaagtatt gattttgca 
      ggttttcac taaacctgc attgcagca tcttcaaac 
      cgaagttac agaatttat tcttgaaca aaaatgttg 
      tttaaagtg gtgaaatgc ccctcagat gagctgtca 
      ttgacaaat tgtgctgtc gtgaatgac gccgacttt 
      tcacaatct acagagcat attgaagtc atcacagga 
      ggagtgaaa aagttcatt ttttccatt aagaaagac 
      ccgagagtt cacaggaat gaggttgcg ttcagtgta 
      ccacagaga aaatgggcc accttgtcc attaatgat 
      gacattgaa gttaatcct tacaaggaa gaagattac 
      ataacttcg atcgtccta gaggctgat tacatgcaa 
      aagaaaaca acatcgcaa gagcaatat gacacagat 
      gaaatggct aaagaattt atttactcg tttccaaat 
      cagatgttt actgttggt caacaactg gctttcctc 
      tttaaagac aaaaagata cttagcctg ctcgtcaag 
      gacattgaa gttgtcaac atcaacacc ctcaagcag 
      ggaggcgag gccaagccc aaaaaaggt aaactggga 
      aaattgaca cccaacact gtcatacag tttgaaaag 
      gccgaaact tcggccctt aatttagta ggcaaagcc 
      aaaggcaaa attgtgcgt cagtcgata attaaccct 
      gattgggac ttccaaaaa atgggaatc ggcggtttg 
      ggcaaggaa tttaacgct atattcaga agagctttt 
      gcgtcaaga gttttcccg ccagagatt gtggaacaa 
      ctgggctgc aaacacgtc aaaggtatt cttctctat 
      ggccctcca ggaactggt aaaactttg atggcgcga 
      caaattgga aaaatgtta aatgccaga gagccgaaa 
      atagtcaac gggccccaa attctagat aaatatgtg 
      ggcgagtct gaggctaat atcagaaga ttgtttgca 
      gaagctgaa gaagaagaa aaaagagcc gggccaaac 
      agtggtttg cacatcatt atctttgat gaaatcgat 
      gcgatttgt aagcaaaga ggatctgtt gctggtaac 
      acaggtgtt cacgatacg gtcgtgaac caattgttg 
      tcaaaaatc gatggagtg gagcaactt aacaatatt 
      cttgtcata gggatgaca aacagacgc gatatgatt 
      gatgaagct ttgctcaga cctggtaga ttggaagta 
      caggtcgag ataagtttg ccaaatgag gatggacga 
      ttcgagatt ttgaatatt catacgagc agaatgagg 
      cagtataag aaaattagt tctgatgtt gacacaagg 
      gaactggca acgttaact aaaaacttc tccggcgcc 
      gaacttgaa ggtttagtc agagcagcc caatccaca 
      gccatgaac cgcttgatc aaagcttcc aataaagtc 
      gaagtagat ccagaagca attagtaaa ttattagta 
      acacgtggc gacttttta aatgcttta gaaaacgac 
      atcaaacca gctttcggc acagcttta gaaatttta 
      gaacagttc ttagcacgt ggaattact gtatggggc 
      agcccagtc agtagcatt ctagaagat ggtcggtta 
      tttatccag caagctaaa gccaccgac acttcaggt 
      ttggtgtcc gttctgatc gagggtcca ccgaacgct 
      ggcaaaacc gctttagcc gcgcagctt gccaaagaa 
      tccgatttt cccttcgtt aaagtgtgc tcacctgaa 
      gaaatggtg ggttatact gaaactgcc aaatgctta 
      catatcagg aaagtgttt gatgatgct taccgctca 
      acactgagt tgcattcta gtcgataat attgaacga 
      ttattagat tacggaccg attggtccc cgatattcc 
      aatttaaca ttacaggca cttttagta ttgttaaag 
      aaacagcca ccgcctgga aaaaaattg ttgattttg 
      tgtacaact agtcgaagg caagtttta gaagatatg 
      gaaatgtta tccgccttc actgctgtt ttacattta 
      ccaaatctt tcaaaagcg gatcagtta ctcacagtt 
      cttgagaat tccgaagtt tttagtcaa caagaattg 
      aagaagatc agccaacaa gtgcacggc aaacgcatt 
      ttcattggg ataaagaaa ctcctggct ctgattgac 
      atggcccgc cagaccgac gaaaagttc cgagtgatg 
      aaattgatc acaaaattg gaggaggac ggtgcactg 
      gaagatccc gggatggcc taatatacc aaatacatg 
      attttacag atataaggt tatgataat cgtgcacat 
      tccacacat ttagcaata tcttgatca atagagtca 
      attgttatt cccggagga aaacatcct aaattatag 
      tgcaattaa ttaattgat ttttctatt tttgcgtcc 
      attgttcat aatttgaag ctttttttg aagattgtt 
      attgttggt tgatagttg tttttgtaa cttccaaag 
      ttttttaat cagtgttag tgtctagaa agtttagcc 
      aacacttga atggtatca cagttagtt acacttaaa 
      ttctttgtt tggtaagtg tctagtaaa tatgtgtgt 
      attatagaa attaattac ttcttatgt ggtctatac 
      tgtatctgc ttagcttgt attcgaagt caataatgt 
      taaaaatag agttaaact tttttgaga tctcgtgtg 
      aatgtttga tagaggatt gttgacgaa aaaggttca 
      aattatttt gtaggtcgt agtcccatt cctaacata 
      gcagactat aactgttaa agatattga atattatat 
      gtatataca ggatgtttc agctttaga atagatgta 
      ttcaaccat ctacctcat ccgtgagta aaactggat 
      gactacaaa cctgtttca tactgtttt aagacacca 
      agttagaat tgttgtgtt acacattgt taaaatgta 
      tgaaacttg gatagtgtt aagaaaaaa atgaagcat 
      cctatatag cattgttgt caataatga tgtataatg 
      aatttattt attaatata aagtcagcc atgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY