Status
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Gene information
General information
TC012274 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3564758..3597022
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012274
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_06409

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012274 RA
        MLERDKRTL FAWILTSSM SSLVYSLET EEPITVNKA 
        RTWARFSTD YVRCFCNLP QCVSTGYMC LSSEGGCFS 
        SFLDHAGHH SKDYRGRHG CLEFLSEEN RAICAAASN 
        TKKTLTLCC HHDMCNHVD SPQTKDIIN NTQQEPHAT 
        IGNLQQPID YSNSQVWFR AATIAVPVC GAVILFVLI 
        ALAVKILRS ENQNHATQK LGTSYEHHM PSQSKHGCD 
        KLERTYDNI LRRSQHQKC LYQYQGDDL QRIQVPLLI 
        QNEIGTHHT GNKNETNAK LNLMQGDAN VASVVLDIE 
        KITPKCSKT NLVHHVNSD KKYIEDKFT SDDKNFINS 
        LNH
      CDS sequence
      • >TC012274 RA
        atgttggag cgtgataaa aggacttta ttcgcctgg 
        atattgact agctcgatg tcttcgttg gtttattcg 
        ctggaaacc gaagagccc atcaccgta aataaagca 
        cggacgtgg gcccgcttt tccacagat tatgtgcgg 
        tgtttctgc aacctcccg cagtgcgtc agcactggt 
        tatatgtgc ctatccagc gagggcggc tgtttttcc 
        agttttctg gaccatgcg gggcatcat tccaaagac 
        tatcgcgga cgtcatggg tgcctggaa tttctcagc 
        gaagaaaat cgtgccatt tgtgctgcc gcttccaac 
        acgaaaaag acactcact ctctgttgc caccacgac 
        atgtgcaac cacgtcgac agcccccaa acaaaagac 
        ataatcaac aacacccaa caagaaccc catgccacc 
        atcggcaac ttacaacaa cccatagac tattcaaac 
        tcccaagtg tggttccga gcagccacc atagcagtg 
        cccgtttgt ggagcagtc attctcttc gtcttgatc 
        gcactagca gttaagatt ttacgaagc gaaaatcaa 
        aatcacgcc acacaaaaa ttagggacg tcatatgaa 
        caccatatg ccttctcaa agcaaacac ggctgtgac 
        aaactggaa cgcacttac gacaacatt ttgcgaaga 
        tcgcagcac cagaaatgt ttatatcag taccaaggc 
        gatgatttg cagcgaatt caagtgccg ctacttatc 
        caaaatgaa attgggacg catcacacg gggaataaa 
        aacgaaacc aacgccaaa ctgaatctg atgcaagga 
        gacgcaaat gtggcgtcg gtcgtgcta gacattgag 
        aagatcacg cccaagtgc tccaaaacc aatttagtc 
        catcatgta aatagcgac aaaaaatac atagaggat 
        aagttcaca agcgacgac aagaacttt atcaactcg 
        cttaatcac tga
      mRNA sequence
      • >TC012274 RA
        gcgaacaaa ataaatttc agttttcag ttcttgagc 
        gtctgttgt tgagtgtgg aggtttggt cagagtacg 
        acgcgttcg gtgcgtgtt cggcgaccc atttccata 
        agaattatt tacttttaa taaatgtaa tttctgcag 
        tgtgtaaag ctgtcatcg gacatgata aacatgtaa 
        tgcgggata atttatttt taattacat atttgtttg 
        atctgactg gcgccaccg cggcatgaa cacgcgctt 
        gtttgaaaa gtttgtttt aaaataaac acagggtgg 
        aagcaatgt tggagcgtg ataaaagga ctttattcg 
        cctggatat tgactagct cgatgtctt cgttggttt 
        attcgctgg aaaccgaag agcccatca ccgtaaata 
        aagcacgga cgtgggccc gcttttcca cagattatg 
        tgcggtgtt tctgcaacc tcccgcagt gcgtcagca 
        ctggttata tgtgcctat ccagcgagg gcggctgtt 
        tttccagtt ttctggacc atgcggggc atcattcca 
        aagactatc gcggacgtc atgggtgcc tggaatttc 
        tcagcgaag aaaatcgtg ccatttgtg ctgccgctt 
        ccaacacga aaaagacac tcactctct gttgccacc 
        acgacatgt gcaaccacg tcgacagcc cccaaacaa 
        aagacataa tcaacaaca cccaacaag aaccccatg 
        ccaccatcg gcaacttac aacaaccca tagactatt 
        caaactccc aagtgtggt tccgagcag ccaccatag 
        cagtgcccg tttgtggag cagtcattc tcttcgtct 
        tgatcgcac tagcagtta agattttac gaagcgaaa 
        atcaaaatc acgccacac aaaaattag ggacgtcat 
        atgaacacc atatgcctt ctcaaagca aacacggct 
        gtgacaaac tggaacgca cttacgaca acattttgc 
        gaagatcgc agcaccaga aatgtttat atcagtacc 
        aaggcgatg atttgcagc gaattcaag tgccgctac 
        ttatccaaa atgaaattg ggacgcatc acacgggga 
        ataaaaacg aaaccaacg ccaaactga atctgatgc 
        aaggagacg caaatgtgg cgtcggtcg tgctagaca 
        ttgagaaga tcacgccca agtgctcca aaaccaatt 
        tagtccatc atgtaaata gcgacaaaa aatacatag 
        aggataagt tcacaagcg acgacaaga actttatca 
        actcgctta atcactgat tgattcaat caacactaa 
        caaggaacc aaagtattt aagtagggt gaacatgtg 
        agtggctat tccacggag gactaaaat gttaggaca 
        ggtggcgaa attgttcct tgcttgtat ttatgttaa 
        aaacaaaca gaaaattac tgttctaga gaactacat 
        tttcttaaa aaatccgaa agactatat gttgaacgt 
        ttttttttt caatcgaaa aaaattgaa acactcgtt 
        tcgaaaaat atggggtgg tgtgcaatt tcaattttt 
        aatattttt tcatgtgtt tcctatgaa aaatttcaa 
        aattacttt ttaaaagaa atacagaaa taataatga 
        acaaaattt ctttagaac cagatgttg cttctaaat 
        taaactatt tgtaaatat agatattta aaagtaagt 
        tttagcggg ttgaaaaat tcagttgtt tattttttt 
        atgaaaata caagttcga agaatgaat caattaatc 
        tgtgcgtag tttaaaaaa atggccaga taaagaagc 
        tacaataat tttttcatt cgatttaaa taagttttt 
        ctaaaaaat taaaacttt tgaattttc caacccttg 
        tagatttgt ttaagttaa gaatgatat attaactga 
        tctgtgaga tgattttgt aatttagaa gcaacatac 
        acagggtgg tttttaaaa aaacggcag gcgctgtag 
        aacttttat ttatggccc gaataataa aaaataaat 
        aaaacgaat ggcttctaa tgtattata agctgatga 
        ttttaattt ttttctaaa tgtcatagt acattcaac 
        ttattttaa attgcgact catattttt cttttaata 
        ttttaaaga tgttagtta tttagtaca aacatgtgt 
        gtttatact aaatttttg ataaaaaaa tattaaaaa 
        tgaagaaca tttaataaa agttaataa aatctaata 
        aaaactatt ctttaaaat aaagatttt aactactta 
        atagatatg ttaatttat atattttgt tatttgctg 
        cgttttgac ataacaata acagcagtg tcttatatg 
        acaccatac aactagatt ttattaata tttttgaaa 
        tttggtaat tttttaaag tagaacata ttgcacatg 
        tttgtaaaa aaataatat attcaattt ata
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY