| General information | |||
| TC012258 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 4467579..4472206 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012258 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06405 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC012258 RA
MGNLQTSEP KNAPKAGKA QGKVKSLIK IRGKKGKID SAQFTGVVP EEAEDSALD DSLPVAEKP KSSTDKIVV TDSWCKVNR LTEDVEKKT KTPPSAVSS SSDSNFTDP LTPMGFNTE MNQCYYSEE SVNLDVEVP NSTTQEEFL HNLTLNSFK LNEYRARHE EEKSKKLSK LGVSKTSQI SLDSDPKDS FVSDNVEVV NKDEEPDSG TLKRKSRDS GEEQKVAHM VKRMSDNNL SNGHVEYKR HMSVPAEIT KPSPDGSVL KKVASLNLN KAELEAKIS KPRFVPEKL DFQLYEKFE GHMLVNWYL SEFSEDHHL VQLLNGQEL KLLAIQFCT HLLAAGVLR QIPDKDVPM YNIFKPDCM YYWAHAETP MSVPQTPGR LSMVSWPPT SPSEAFLTA SINDAATAS SPKSPELPF GEPEERPRS NQEFKNSAR DVEILALEE EINRLKQEV ENPTIPTPP PPPPPPMPG IGAPPPPPM PGIGAPPPP PMPGIGAHP PPPMPGIVG PPPPPMPGI GGPPPPPMP GTGPPPPPP PMGGVPPPP PPMGGPVPL PPPPAGGWS SQKAVVPPV PVSLGLRKA TLNPKVPMK PLYWTRILA PAGTDSVNS PSSDALWTQ IDELPLDSL NEFVDLFSR QVVTRKPTV KKQEQKAKA EAVKLLDSK RSQNVGILA QSLHVDFQE IENAIYNFD TSIVSLEAL QQIYEVRAT AEELELIKN HLSTKPNIP LDKPEQFLH ELSEISNFA DRIACLMFQ VEFDDSINT IGHTLTNIK TTCDYLVNS NELKEVFAI ILTLGNYMN GGNMTRGQA DGFGLEILP KLKDVKSKD SKVTLLHYI VKIYMKKIE NPFEPNVVL PVPEPGDIE RAASVNFDD VKVNLQKLE KQLTECEKK IQKVIDSSN PDNLQPFKD KMTTFLEHS KKQLATEFE NLEECKVKF IKTMKFYLF KPKSGTLED YPPNSFFEM WLPFCIDFK DIFKKELIR MEKEKIQEM RKRENERVS EPIKIKERE NGLKSKVKK LQAKHASQV ETN
- >TC012258 RB
MGNLQTSEP KNAPKAGKA QGKVKSLIK IRGKKGKID SAQFTGVVP EEAEDSALD DSLPVAEKP KSSTDKIVV TDSWCKVNR LTEDVEKKT KTPPSAVSS SSDSNFTDP LTPMGFNTE MNQCYYSEE SVNLDVEVP NSTTQEEFL HNLTLNSFK LNEYRARHE EEKSKKLSK LGVSKTSQI SLDSDPKDS FVSDNVEVV NKDEEPDSG TLKRKSRDS GEEQKVAHM VKRMSDNNL SNGHVEYKR HMSVPAEIT KPSPDGSVL KKVASLNLN KAELEAKIS KPRFVPEKL DFQLYEKFE GHMLVNWYL SEFSEDHHL VQLLNGQEL KLLAIQFCT HLLAAGVLR QIPDKDVPM YNIFKPDCM YYWAHAETP MSVPQTPGR LSMVSWPPT SPSEAFLTA SINDAATAS SPKSPELPF GEPEERPRS NQEFKNSAR DVEILALEE EINRLKQEV ENPTIPTPP PPPPPPMPG IGAPPPPPM PGIGAPPPP PMPGIGAHP PPPMPGIVG PPPPPMPGI GGPPPPPMP GTGPPPPPP PMGGVPPPP PPMGGPVPL PPPPAGGWS SQKAGLRKA TLNPKVPMK PLYWTRILA PAGTDSVNS PSSDALWTQ IDELPLDSL NEFVDLFSR QVVTRKPTV KKQEQKAKA EAVKLLDSK RSQNVGILA QSLHVDFQE IENAIYNFD TSIVSLEAL QQIYEVRAT AEELELIKN HLSTKPNIP LDKPEQFLH ELSEISNFA DRIACLMFQ VEFDDSINT IGHTLTNIK TTCDYLVNS NELKEVFAI ILTLGNYMN GGNMTRGQA DGFGLEILP KLKDVKSKD SKVTLLHYI VKIYMKKIE NPFEPNVVL PVPEPGDIE RAASVNFDD VKVNLQKLE KQLTECEKK IQKVIDSSN PDNLQPFKD KMTTFLEHS KKQLATEFE NLEECKVKF IKTMKFYLF KPKSGTLED YPPNSFFEM WLPFCIDFK DIFKKELIR MEKEKIQEM RKRENERVS EPIKIKERE NGLKSKVKK LQAKHASQV ETN
- >TC012258 RA
atggggaac ttgcaaacg agcgagcct aaaaacgcc cccaaggcc ggcaaagcc caaggtaaa gtaaagtct ttgataaaa atcagggga aaaaagggc aaaatcgac tcggcacaa ttcaccgga gtggtccct gaagaagcc gaagactca gccctggac gactcgctc ccggtcgca gaaaagccg aaatccagc accgacaag atcgtagtg accgactcc tggtgcaaa gtcaaccgc ttgactgaa gacgtcgag aagaaaacc aaaacgcca ccgagcgcc gtctcctcg agctccgac tcgaacttc acggaccct ttgacacca atgggcttc aacaccgaa atgaaccag tgctactac agcgaggag agcgtcaac ctggacgtc gaagtcccc aacagcacc acccaggag gagttcctc cacaacctc accctcaac agcttcaaa ttgaacgaa tatcgcgcc aggcacgag gaggagaag tcgaaaaag ttgagcaaa ctcggagtg tccaaaaca agccaaatt agtctggac agcgatccg aaggacagt ttcgtgtcg gacaacgtc gaagttgtt aacaaggac gaagagccc gatagcggc acgctgaag aggaagtcg cgagatagc ggggaggaa caaaaagtc gcgcacatg gttaagagg atgtccgat aataattta tcaaacggg catgtggag tacaagaga cacatgtcg gtccccgct gaaatcacg aagccgagt ccagatggt tcagtattg aaaaaagtc gcctcgctt aatttaaac aaagccgaa cttgaggca aagatttca aagccgagg tttgtgccc gaaaaactg gattttcaa ctgtacgaa aaattcgaa ggtcacatg ttggtgaat tggtacttg tctgagttc agtgaagac caccacttg gttcagttg ctcaacggc caagagttg aagcttctg gcgatccag ttctgcact cacctcctg gccgctggc gttttgcgt caaatccca gacaaagat gtcccaatg tacaacata ttcaaaccc gattgcatg tactactgg gcccatgcg gagactcca atgtccgtc cctcagaca ccgggaagg ctcagcatg gtttcgtgg cctccgaca tcgccatcg gaggctttc ctcacagcg agcataaac gacgccgcc acggcatca tcacctaaa agtcctgaa ctgccattc ggggaaccc gaagagcga cctaggagc aaccaagag ttcaaaaac tcggcccga gacgtcgaa atcctagca ctagaggaa gaaataaat cggttgaag caggaagta gagaacccc actatccca accccgccc ccacctcct cctccccca atgccggga attggagcg cccccacct cctccaatg ccgggaatt ggagcgccc ccacctcct ccaatgccg ggaattgga gcgcaccca ccacctcca atgcccggg attgtcgga cccccaccg ccccccatg ccgggaata gggggccct ccaccgccc ccaatgccg ggcacgggg ccgccgccc cctccgccc ccaatgggc ggtgtgcct ccgcccccg ccgccgatg ggagggcca gtgccgctg ccgccgccc ccagccggg gggtggagt tcgcaaaag gcagtggtc ccccccgtt ccagtgtcc ttaggattg cgaaaagcc actttaaat ccgaaagta ccgatgaag cctttgtat tggaccaga atcctggca cctgctgga acagatagt gtcaattct ccgtcatct gatgctctt tggacacaa atagatgaa ttaccttta gatagttta aatgaattc gtggattta ttttcaagg caagttgtt acgagaaaa cctacggtg aagaaacaa gaacagaag gctaaagct gaagctgtc aagcttttg gatagtaaa agatcacaa aatgttgga atccttgct caaagttta cacgtagat ttccaagaa attgaaaat gctatttac aattttgat acttctatc gttagttta gaagctttg caacagatt tacgaagtt agagcaact gcagaagaa ttagaactt attaaaaac catctatca acgaaacct aacatacca ttagacaag cctgaacag tttttacac gaattatcg gaaatatca aatttcgcg gatagaatc gcttgctta atgtttcaa gttgaattc gacgattct atcaatact atagggcat actttaaca aatataaaa actacttgt gattactta gttaatagt aatgaatta aaagaagtt tttgcaata attttaact ctgggaaat tatatgaac ggcggcaat atgacgcgt ggacaagcc gacgggttc ggcttagaa attttacca aaattaaaa gacgttaaa tcgaaagat tccaaagtc acgcttctc cactacatt gtcaaaatc tacatgaaa aaaatcgaa aacccgttt gaacccaat gttgtacta cccgtaccg gaacctgga gatatcgaa cgcgcagct tccgtaaat tttgacgat gtcaaagta aatttacaa aaactggaa aaacaacta acagagtgt gagaaaaaa atccagaaa gtgattgac tcatcaaac cccgacaat cttcaacct ttcaaggat aagatgacc acgttcttg gaacactcg aagaaacag cttgcaaca gaattcgaa aatttggaa gagtgcaaa gtcaagttc atcaagacg atgaagttt tacttgttt aagcccaaa tcggggact ttggaagat tatcccccg aactcattt ttcgaaatg tggctacca ttctgcatc gactttaaa gatatcttc aagaaggaa ctgatcaga atggaaaaa gaaaagatc caagaaatg aggaaacgc gaaaacgag cgagtgtcg gaaccaata aagataaaa gagcgcgaa aacggacta aaatcgaaa gtcaagaaa ctccaagcc aaacacgct tcacaggtg gagaccaat taa
- >TC012258 RB
atggggaac ttgcaaacg agcgagcct aaaaacgcc cccaaggcc ggcaaagcc caaggtaaa gtaaagtct ttgataaaa atcagggga aaaaagggc aaaatcgac tcggcacaa ttcaccgga gtggtccct gaagaagcc gaagactca gccctggac gactcgctc ccggtcgca gaaaagccg aaatccagc accgacaag atcgtagtg accgactcc tggtgcaaa gtcaaccgc ttgactgaa gacgtcgag aagaaaacc aaaacgcca ccgagcgcc gtctcctcg agctccgac tcgaacttc acggaccct ttgacacca atgggcttc aacaccgaa atgaaccag tgctactac agcgaggag agcgtcaac ctggacgtc gaagtcccc aacagcacc acccaggag gagttcctc cacaacctc accctcaac agcttcaaa ttgaacgaa tatcgcgcc aggcacgag gaggagaag tcgaaaaag ttgagcaaa ctcggagtg tccaaaaca agccaaatt agtctggac agcgatccg aaggacagt ttcgtgtcg gacaacgtc gaagttgtt aacaaggac gaagagccc gatagcggc acgctgaag aggaagtcg cgagatagc ggggaggaa caaaaagtc gcgcacatg gttaagagg atgtccgat aataattta tcaaacggg catgtggag tacaagaga cacatgtcg gtccccgct gaaatcacg aagccgagt ccagatggt tcagtattg aaaaaagtc gcctcgctt aatttaaac aaagccgaa cttgaggca aagatttca aagccgagg tttgtgccc gaaaaactg gattttcaa ctgtacgaa aaattcgaa ggtcacatg ttggtgaat tggtacttg tctgagttc agtgaagac caccacttg gttcagttg ctcaacggc caagagttg aagcttctg gcgatccag ttctgcact cacctcctg gccgctggc gttttgcgt caaatccca gacaaagat gtcccaatg tacaacata ttcaaaccc gattgcatg tactactgg gcccatgcg gagactcca atgtccgtc cctcagaca ccgggaagg ctcagcatg gtttcgtgg cctccgaca tcgccatcg gaggctttc ctcacagcg agcataaac gacgccgcc acggcatca tcacctaaa agtcctgaa ctgccattc ggggaaccc gaagagcga cctaggagc aaccaagag ttcaaaaac tcggcccga gacgtcgaa atcctagca ctagaggaa gaaataaat cggttgaag caggaagta gagaacccc actatccca accccgccc ccacctcct cctccccca atgccggga attggagcg cccccacct cctccaatg ccgggaatt ggagcgccc ccacctcct ccaatgccg ggaattgga gcgcaccca ccacctcca atgcccggg attgtcgga cccccaccg ccccccatg ccgggaata gggggccct ccaccgccc ccaatgccg ggcacgggg ccgccgccc cctccgccc ccaatgggc ggtgtgcct ccgcccccg ccgccgatg ggagggcca gtgccgctg ccgccgccc ccagccggg gggtggagt tcgcaaaag gcaggattg cgaaaagcc actttaaat ccgaaagta ccgatgaag cctttgtat tggaccaga atcctggca cctgctgga acagatagt gtcaattct ccgtcatct gatgctctt tggacacaa atagatgaa ttaccttta gatagttta aatgaattc gtggattta ttttcaagg caagttgtt acgagaaaa cctacggtg aagaaacaa gaacagaag gctaaagct gaagctgtc aagcttttg gatagtaaa agatcacaa aatgttgga atccttgct caaagttta cacgtagat ttccaagaa attgaaaat gctatttac aattttgat acttctatc gttagttta gaagctttg caacagatt tacgaagtt agagcaact gcagaagaa ttagaactt attaaaaac catctatca acgaaacct aacatacca ttagacaag cctgaacag tttttacac gaattatcg gaaatatca aatttcgcg gatagaatc gcttgctta atgtttcaa gttgaattc gacgattct atcaatact atagggcat actttaaca aatataaaa actacttgt gattactta gttaatagt aatgaatta aaagaagtt tttgcaata attttaact ctgggaaat tatatgaac ggcggcaat atgacgcgt ggacaagcc gacgggttc ggcttagaa attttacca aaattaaaa gacgttaaa tcgaaagat tccaaagtc acgcttctc cactacatt gtcaaaatc tacatgaaa aaaatcgaa aacccgttt gaacccaat gttgtacta cccgtaccg gaacctgga gatatcgaa cgcgcagct tccgtaaat tttgacgat gtcaaagta aatttacaa aaactggaa aaacaacta acagagtgt gagaaaaaa atccagaaa gtgattgac tcatcaaac cccgacaat cttcaacct ttcaaggat aagatgacc acgttcttg gaacactcg aagaaacag cttgcaaca gaattcgaa aatttggaa gagtgcaaa gtcaagttc atcaagacg atgaagttt tacttgttt aagcccaaa tcggggact ttggaagat tatcccccg aactcattt ttcgaaatg tggctacca ttctgcatc gactttaaa gatatcttc aagaaggaa ctgatcaga atggaaaaa gaaaagatc caagaaatg aggaaacgc gaaaacgag cgagtgtcg gaaccaata aagataaaa gagcgcgaa aacggacta aaatcgaaa gtcaagaaa ctccaagcc aaacacgct tcacaggtg gagaccaat taa
- >TC012258 RA
aaaattaca caaatagtg agcgaaaac cggaagttc agttaatta tggaactcg ttacaaaat tgcagtgtt tatttgtga tcccacttt ccagttcga gtgattaca cgtttgtgt ttttgttaa agacattca aaaatattt agagttgtt gtaaattaa gccaatata gaaaaatgg ggaacttgc aaacgagcg agcctaaaa acgccccca aggccggca aagcccaag gtaaagtaa agtctttga taaaaatca ggggaaaaa agggcaaaa tcgactcgg cacaattca ccggagtgg tccctgaag aagccgaag actcagccc tggacgact cgctcccgg tcgcagaaa agccgaaat ccagcaccg acaagatcg tagtgaccg actcctggt gcaaagtca accgcttga ctgaagacg tcgagaaga aaaccaaaa cgccaccga gcgccgtct cctcgagct ccgactcga acttcacgg accctttga caccaatgg gcttcaaca ccgaaatga accagtgct actacagcg aggagagcg tcaacctgg acgtcgaag tccccaaca gcaccaccc aggaggagt tcctccaca acctcaccc tcaacagct tcaaattga acgaatatc gcgccaggc acgaggagg agaagtcga aaaagttga gcaaactcg gagtgtcca aaacaagcc aaattagtc tggacagcg atccgaagg acagtttcg tgtcggaca acgtcgaag ttgttaaca aggacgaag agcccgata gcggcacgc tgaagagga agtcgcgag atagcgggg aggaacaaa aagtcgcgc acatggtta agaggatgt ccgataata atttatcaa acgggcatg tggagtaca agagacaca tgtcggtcc ccgctgaaa tcacgaagc cgagtccag atggttcag tattgaaaa aagtcgcct cgcttaatt taaacaaag ccgaacttg aggcaaaga tttcaaagc cgaggtttg tgcccgaaa aactggatt ttcaactgt acgaaaaat tcgaaggtc acatgttgg tgaattggt acttgtctg agttcagtg aagaccacc acttggttc agttgctca acggccaag agttgaagc ttctggcga tccagttct gcactcacc tcctggccg ctggcgttt tgcgtcaaa tcccagaca aagatgtcc caatgtaca acatattca aacccgatt gcatgtact actgggccc atgcggaga ctccaatgt ccgtccctc agacaccgg gaaggctca gcatggttt cgtggcctc cgacatcgc catcggagg ctttcctca cagcgagca taaacgacg ccgccacgg catcatcac ctaaaagtc ctgaactgc cattcgggg aacccgaag agcgaccta ggagcaacc aagagttca aaaactcgg cccgagacg tcgaaatcc tagcactag aggaagaaa taaatcggt tgaagcagg aagtagaga accccacta tcccaaccc cgcccccac ctcctcctc ccccaatgc cgggaattg gagcgcccc cacctcctc caatgccgg gaattggag cgcccccac ctcctccaa tgccgggaa ttggagcgc acccaccac ctccaatgc ccgggattg tcggacccc caccgcccc ccatgccgg gaatagggg gccctccac cgcccccaa tgccgggca cggggccgc cgccccctc cgcccccaa tgggcggtg tgcctccgc ccccgccgc cgatgggag ggccagtgc cgctgccgc cgcccccag ccggggggt ggagttcgc aaaaggcag tggtccccc ccgttccag tgtccttag gattgcgaa aagccactt taaatccga aagtaccga tgaagcctt tgtattgga ccagaatcc tggcacctg ctggaacag atagtgtca attctccgt catctgatg ctctttgga cacaaatag atgaattac ctttagata gtttaaatg aattcgtgg atttatttt caaggcaag ttgttacga gaaaaccta cggtgaaga aacaagaac agaaggcta aagctgaag ctgtcaagc ttttggata gtaaaagat cacaaaatg ttggaatcc ttgctcaaa gtttacacg tagatttcc aagaaattg aaaatgcta tttacaatt ttgatactt ctatcgtta gtttagaag ctttgcaac agatttacg aagttagag caactgcag aagaattag aacttatta aaaaccatc tatcaacga aacctaaca taccattag acaagcctg aacagtttt tacacgaat tatcggaaa tatcaaatt tcgcggata gaatcgctt gcttaatgt ttcaagttg aattcgacg attctatca atactatag ggcatactt taacaaata taaaaacta cttgtgatt acttagtta atagtaatg aattaaaag aagtttttg caataattt taactctgg gaaattata tgaacggcg gcaatatga cgcgtggac aagccgacg ggttcggct tagaaattt taccaaaat taaaagacg ttaaatcga aagattcca aagtcacgc ttctccact acattgtca aaatctaca tgaaaaaaa tcgaaaacc cgtttgaac ccaatgttg tactacccg taccggaac ctggagata tcgaacgcg cagcttccg taaattttg acgatgtca aagtaaatt tacaaaaac tggaaaaac aactaacag agtgtgaga aaaaaatcc agaaagtga ttgactcat caaaccccg acaatcttc aacctttca aggataaga tgaccacgt tcttggaac actcgaaga aacagcttg caacagaat tcgaaaatt tggaagagt gcaaagtca agttcatca agacgatga agttttact tgtttaagc ccaaatcgg ggactttgg aagattatc ccccgaact catttttcg aaatgtggc taccattct gcatcgact ttaaagata tcttcaaga aggaactga tcagaatgg aaaaagaaa agatccaag aaatgagga aacgcgaaa acgagcgag tgtcggaac caataaaga taaaagagc gcgaaaacg gactaaaat cgaaagtca agaaactcc aagccaaac acgcttcac aggtggaga ccaattaat acttataca aagtttacg ttatgtatt tgttacaaa aataagttt tatttattg ttttacgtc gaatattac ctgtgtt
- >TC012258 RB
aaaattaca caaatagtg agcgaaaac cggaagttc agttaatta tggaactcg ttacaaaat tgcagtgtt tatttgtga tcccacttt ccagttcga gtgattaca cgtttgtgt ttttgttaa agacattca aaaatattt agagttgtt gtaaattaa gccaatata gaaaaatgg ggaacttgc aaacgagcg agcctaaaa acgccccca aggccggca aagcccaag gtaaagtaa agtctttga taaaaatca ggggaaaaa agggcaaaa tcgactcgg cacaattca ccggagtgg tccctgaag aagccgaag actcagccc tggacgact cgctcccgg tcgcagaaa agccgaaat ccagcaccg acaagatcg tagtgaccg actcctggt gcaaagtca accgcttga ctgaagacg tcgagaaga aaaccaaaa cgccaccga gcgccgtct cctcgagct ccgactcga acttcacgg accctttga caccaatgg gcttcaaca ccgaaatga accagtgct actacagcg aggagagcg tcaacctgg acgtcgaag tccccaaca gcaccaccc aggaggagt tcctccaca acctcaccc tcaacagct tcaaattga acgaatatc gcgccaggc acgaggagg agaagtcga aaaagttga gcaaactcg gagtgtcca aaacaagcc aaattagtc tggacagcg atccgaagg acagtttcg tgtcggaca acgtcgaag ttgttaaca aggacgaag agcccgata gcggcacgc tgaagagga agtcgcgag atagcgggg aggaacaaa aagtcgcgc acatggtta agaggatgt ccgataata atttatcaa acgggcatg tggagtaca agagacaca tgtcggtcc ccgctgaaa tcacgaagc cgagtccag atggttcag tattgaaaa aagtcgcct cgcttaatt taaacaaag ccgaacttg aggcaaaga tttcaaagc cgaggtttg tgcccgaaa aactggatt ttcaactgt acgaaaaat tcgaaggtc acatgttgg tgaattggt acttgtctg agttcagtg aagaccacc acttggttc agttgctca acggccaag agttgaagc ttctggcga tccagttct gcactcacc tcctggccg ctggcgttt tgcgtcaaa tcccagaca aagatgtcc caatgtaca acatattca aacccgatt gcatgtact actgggccc atgcggaga ctccaatgt ccgtccctc agacaccgg gaaggctca gcatggttt cgtggcctc cgacatcgc catcggagg ctttcctca cagcgagca taaacgacg ccgccacgg catcatcac ctaaaagtc ctgaactgc cattcgggg aacccgaag agcgaccta ggagcaacc aagagttca aaaactcgg cccgagacg tcgaaatcc tagcactag aggaagaaa taaatcggt tgaagcagg aagtagaga accccacta tcccaaccc cgcccccac ctcctcctc ccccaatgc cgggaattg gagcgcccc cacctcctc caatgccgg gaattggag cgcccccac ctcctccaa tgccgggaa ttggagcgc acccaccac ctccaatgc ccgggattg tcggacccc caccgcccc ccatgccgg gaatagggg gccctccac cgcccccaa tgccgggca cggggccgc cgccccctc cgcccccaa tgggcggtg tgcctccgc ccccgccgc cgatgggag ggccagtgc cgctgccgc cgcccccag ccggggggt ggagttcgc aaaaggcag gattgcgaa aagccactt taaatccga aagtaccga tgaagcctt tgtattgga ccagaatcc tggcacctg ctggaacag atagtgtca attctccgt catctgatg ctctttgga cacaaatag atgaattac ctttagata gtttaaatg aattcgtgg atttatttt caaggcaag ttgttacga gaaaaccta cggtgaaga aacaagaac agaaggcta aagctgaag ctgtcaagc ttttggata gtaaaagat cacaaaatg ttggaatcc ttgctcaaa gtttacacg tagatttcc aagaaattg aaaatgcta tttacaatt ttgatactt ctatcgtta gtttagaag ctttgcaac agatttacg aagttagag caactgcag aagaattag aacttatta aaaaccatc tatcaacga aacctaaca taccattag acaagcctg aacagtttt tacacgaat tatcggaaa tatcaaatt tcgcggata gaatcgctt gcttaatgt ttcaagttg aattcgacg attctatca atactatag ggcatactt taacaaata taaaaacta cttgtgatt acttagtta atagtaatg aattaaaag aagtttttg caataattt taactctgg gaaattata tgaacggcg gcaatatga cgcgtggac aagccgacg ggttcggct tagaaattt taccaaaat taaaagacg ttaaatcga aagattcca aagtcacgc ttctccact acattgtca aaatctaca tgaaaaaaa tcgaaaacc cgtttgaac ccaatgttg tactacccg taccggaac ctggagata tcgaacgcg cagcttccg taaattttg acgatgtca aagtaaatt tacaaaaac tggaaaaac aactaacag agtgtgaga aaaaaatcc agaaagtga ttgactcat caaaccccg acaatcttc aacctttca aggataaga tgaccacgt tcttggaac actcgaaga aacagcttg caacagaat tcgaaaatt tggaagagt gcaaagtca agttcatca agacgatga agttttact tgtttaagc ccaaatcgg ggactttgg aagattatc ccccgaact catttttcg aaatgtggc taccattct gcatcgact ttaaagata tcttcaaga aggaactga tcagaatgg aaaaagaaa agatccaag aaatgagga aacgcgaaa acgagcgag tgtcggaac caataaaga taaaagagc gcgaaaacg gactaaaat cgaaagtca agaaactcc aagccaaac acgcttcac aggtggaga ccaattaat acttataca aagtttacg ttatgtatt tgttacaaa aataagttt tatttattg ttttacgtc gaatattac ctgtgtt
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