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Gene information
General information
TC012258 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4467579..4472206
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012258
    iBeetle screen iB_06405

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012258 RA
      MGNLQTSEP KNAPKAGKA QGKVKSLIK IRGKKGKID 
      SAQFTGVVP EEAEDSALD DSLPVAEKP KSSTDKIVV 
      TDSWCKVNR LTEDVEKKT KTPPSAVSS SSDSNFTDP 
      LTPMGFNTE MNQCYYSEE SVNLDVEVP NSTTQEEFL 
      HNLTLNSFK LNEYRARHE EEKSKKLSK LGVSKTSQI 
      SLDSDPKDS FVSDNVEVV NKDEEPDSG TLKRKSRDS 
      GEEQKVAHM VKRMSDNNL SNGHVEYKR HMSVPAEIT 
      KPSPDGSVL KKVASLNLN KAELEAKIS KPRFVPEKL 
      DFQLYEKFE GHMLVNWYL SEFSEDHHL VQLLNGQEL 
      KLLAIQFCT HLLAAGVLR QIPDKDVPM YNIFKPDCM 
      YYWAHAETP MSVPQTPGR LSMVSWPPT SPSEAFLTA 
      SINDAATAS SPKSPELPF GEPEERPRS NQEFKNSAR 
      DVEILALEE EINRLKQEV ENPTIPTPP PPPPPPMPG 
      IGAPPPPPM PGIGAPPPP PMPGIGAHP PPPMPGIVG 
      PPPPPMPGI GGPPPPPMP GTGPPPPPP PMGGVPPPP 
      PPMGGPVPL PPPPAGGWS SQKAVVPPV PVSLGLRKA 
      TLNPKVPMK PLYWTRILA PAGTDSVNS PSSDALWTQ 
      IDELPLDSL NEFVDLFSR QVVTRKPTV KKQEQKAKA 
      EAVKLLDSK RSQNVGILA QSLHVDFQE IENAIYNFD 
      TSIVSLEAL QQIYEVRAT AEELELIKN HLSTKPNIP 
      LDKPEQFLH ELSEISNFA DRIACLMFQ VEFDDSINT 
      IGHTLTNIK TTCDYLVNS NELKEVFAI ILTLGNYMN 
      GGNMTRGQA DGFGLEILP KLKDVKSKD SKVTLLHYI 
      VKIYMKKIE NPFEPNVVL PVPEPGDIE RAASVNFDD 
      VKVNLQKLE KQLTECEKK IQKVIDSSN PDNLQPFKD 
      KMTTFLEHS KKQLATEFE NLEECKVKF IKTMKFYLF 
      KPKSGTLED YPPNSFFEM WLPFCIDFK DIFKKELIR 
      MEKEKIQEM RKRENERVS EPIKIKERE NGLKSKVKK 
      LQAKHASQV ETN
    • >TC012258 RB
      MGNLQTSEP KNAPKAGKA QGKVKSLIK IRGKKGKID 
      SAQFTGVVP EEAEDSALD DSLPVAEKP KSSTDKIVV 
      TDSWCKVNR LTEDVEKKT KTPPSAVSS SSDSNFTDP 
      LTPMGFNTE MNQCYYSEE SVNLDVEVP NSTTQEEFL 
      HNLTLNSFK LNEYRARHE EEKSKKLSK LGVSKTSQI 
      SLDSDPKDS FVSDNVEVV NKDEEPDSG TLKRKSRDS 
      GEEQKVAHM VKRMSDNNL SNGHVEYKR HMSVPAEIT 
      KPSPDGSVL KKVASLNLN KAELEAKIS KPRFVPEKL 
      DFQLYEKFE GHMLVNWYL SEFSEDHHL VQLLNGQEL 
      KLLAIQFCT HLLAAGVLR QIPDKDVPM YNIFKPDCM 
      YYWAHAETP MSVPQTPGR LSMVSWPPT SPSEAFLTA 
      SINDAATAS SPKSPELPF GEPEERPRS NQEFKNSAR 
      DVEILALEE EINRLKQEV ENPTIPTPP PPPPPPMPG 
      IGAPPPPPM PGIGAPPPP PMPGIGAHP PPPMPGIVG 
      PPPPPMPGI GGPPPPPMP GTGPPPPPP PMGGVPPPP 
      PPMGGPVPL PPPPAGGWS SQKAGLRKA TLNPKVPMK 
      PLYWTRILA PAGTDSVNS PSSDALWTQ IDELPLDSL 
      NEFVDLFSR QVVTRKPTV KKQEQKAKA EAVKLLDSK 
      RSQNVGILA QSLHVDFQE IENAIYNFD TSIVSLEAL 
      QQIYEVRAT AEELELIKN HLSTKPNIP LDKPEQFLH 
      ELSEISNFA DRIACLMFQ VEFDDSINT IGHTLTNIK 
      TTCDYLVNS NELKEVFAI ILTLGNYMN GGNMTRGQA 
      DGFGLEILP KLKDVKSKD SKVTLLHYI VKIYMKKIE 
      NPFEPNVVL PVPEPGDIE RAASVNFDD VKVNLQKLE 
      KQLTECEKK IQKVIDSSN PDNLQPFKD KMTTFLEHS 
      KKQLATEFE NLEECKVKF IKTMKFYLF KPKSGTLED 
      YPPNSFFEM WLPFCIDFK DIFKKELIR MEKEKIQEM 
      RKRENERVS EPIKIKERE NGLKSKVKK LQAKHASQV 
      ETN
    CDS sequence
    • >TC012258 RA
      atggggaac ttgcaaacg agcgagcct aaaaacgcc 
      cccaaggcc ggcaaagcc caaggtaaa gtaaagtct 
      ttgataaaa atcagggga aaaaagggc aaaatcgac 
      tcggcacaa ttcaccgga gtggtccct gaagaagcc 
      gaagactca gccctggac gactcgctc ccggtcgca 
      gaaaagccg aaatccagc accgacaag atcgtagtg 
      accgactcc tggtgcaaa gtcaaccgc ttgactgaa 
      gacgtcgag aagaaaacc aaaacgcca ccgagcgcc 
      gtctcctcg agctccgac tcgaacttc acggaccct 
      ttgacacca atgggcttc aacaccgaa atgaaccag 
      tgctactac agcgaggag agcgtcaac ctggacgtc 
      gaagtcccc aacagcacc acccaggag gagttcctc 
      cacaacctc accctcaac agcttcaaa ttgaacgaa 
      tatcgcgcc aggcacgag gaggagaag tcgaaaaag 
      ttgagcaaa ctcggagtg tccaaaaca agccaaatt 
      agtctggac agcgatccg aaggacagt ttcgtgtcg 
      gacaacgtc gaagttgtt aacaaggac gaagagccc 
      gatagcggc acgctgaag aggaagtcg cgagatagc 
      ggggaggaa caaaaagtc gcgcacatg gttaagagg 
      atgtccgat aataattta tcaaacggg catgtggag 
      tacaagaga cacatgtcg gtccccgct gaaatcacg 
      aagccgagt ccagatggt tcagtattg aaaaaagtc 
      gcctcgctt aatttaaac aaagccgaa cttgaggca 
      aagatttca aagccgagg tttgtgccc gaaaaactg 
      gattttcaa ctgtacgaa aaattcgaa ggtcacatg 
      ttggtgaat tggtacttg tctgagttc agtgaagac 
      caccacttg gttcagttg ctcaacggc caagagttg 
      aagcttctg gcgatccag ttctgcact cacctcctg 
      gccgctggc gttttgcgt caaatccca gacaaagat 
      gtcccaatg tacaacata ttcaaaccc gattgcatg 
      tactactgg gcccatgcg gagactcca atgtccgtc 
      cctcagaca ccgggaagg ctcagcatg gtttcgtgg 
      cctccgaca tcgccatcg gaggctttc ctcacagcg 
      agcataaac gacgccgcc acggcatca tcacctaaa 
      agtcctgaa ctgccattc ggggaaccc gaagagcga 
      cctaggagc aaccaagag ttcaaaaac tcggcccga 
      gacgtcgaa atcctagca ctagaggaa gaaataaat 
      cggttgaag caggaagta gagaacccc actatccca 
      accccgccc ccacctcct cctccccca atgccggga 
      attggagcg cccccacct cctccaatg ccgggaatt 
      ggagcgccc ccacctcct ccaatgccg ggaattgga 
      gcgcaccca ccacctcca atgcccggg attgtcgga 
      cccccaccg ccccccatg ccgggaata gggggccct 
      ccaccgccc ccaatgccg ggcacgggg ccgccgccc 
      cctccgccc ccaatgggc ggtgtgcct ccgcccccg 
      ccgccgatg ggagggcca gtgccgctg ccgccgccc 
      ccagccggg gggtggagt tcgcaaaag gcagtggtc 
      ccccccgtt ccagtgtcc ttaggattg cgaaaagcc 
      actttaaat ccgaaagta ccgatgaag cctttgtat 
      tggaccaga atcctggca cctgctgga acagatagt 
      gtcaattct ccgtcatct gatgctctt tggacacaa 
      atagatgaa ttaccttta gatagttta aatgaattc 
      gtggattta ttttcaagg caagttgtt acgagaaaa 
      cctacggtg aagaaacaa gaacagaag gctaaagct 
      gaagctgtc aagcttttg gatagtaaa agatcacaa 
      aatgttgga atccttgct caaagttta cacgtagat 
      ttccaagaa attgaaaat gctatttac aattttgat 
      acttctatc gttagttta gaagctttg caacagatt 
      tacgaagtt agagcaact gcagaagaa ttagaactt 
      attaaaaac catctatca acgaaacct aacatacca 
      ttagacaag cctgaacag tttttacac gaattatcg 
      gaaatatca aatttcgcg gatagaatc gcttgctta 
      atgtttcaa gttgaattc gacgattct atcaatact 
      atagggcat actttaaca aatataaaa actacttgt 
      gattactta gttaatagt aatgaatta aaagaagtt 
      tttgcaata attttaact ctgggaaat tatatgaac 
      ggcggcaat atgacgcgt ggacaagcc gacgggttc 
      ggcttagaa attttacca aaattaaaa gacgttaaa 
      tcgaaagat tccaaagtc acgcttctc cactacatt 
      gtcaaaatc tacatgaaa aaaatcgaa aacccgttt 
      gaacccaat gttgtacta cccgtaccg gaacctgga 
      gatatcgaa cgcgcagct tccgtaaat tttgacgat 
      gtcaaagta aatttacaa aaactggaa aaacaacta 
      acagagtgt gagaaaaaa atccagaaa gtgattgac 
      tcatcaaac cccgacaat cttcaacct ttcaaggat 
      aagatgacc acgttcttg gaacactcg aagaaacag 
      cttgcaaca gaattcgaa aatttggaa gagtgcaaa 
      gtcaagttc atcaagacg atgaagttt tacttgttt 
      aagcccaaa tcggggact ttggaagat tatcccccg 
      aactcattt ttcgaaatg tggctacca ttctgcatc 
      gactttaaa gatatcttc aagaaggaa ctgatcaga 
      atggaaaaa gaaaagatc caagaaatg aggaaacgc 
      gaaaacgag cgagtgtcg gaaccaata aagataaaa 
      gagcgcgaa aacggacta aaatcgaaa gtcaagaaa 
      ctccaagcc aaacacgct tcacaggtg gagaccaat 
      taa
    • >TC012258 RB
      atggggaac ttgcaaacg agcgagcct aaaaacgcc 
      cccaaggcc ggcaaagcc caaggtaaa gtaaagtct 
      ttgataaaa atcagggga aaaaagggc aaaatcgac 
      tcggcacaa ttcaccgga gtggtccct gaagaagcc 
      gaagactca gccctggac gactcgctc ccggtcgca 
      gaaaagccg aaatccagc accgacaag atcgtagtg 
      accgactcc tggtgcaaa gtcaaccgc ttgactgaa 
      gacgtcgag aagaaaacc aaaacgcca ccgagcgcc 
      gtctcctcg agctccgac tcgaacttc acggaccct 
      ttgacacca atgggcttc aacaccgaa atgaaccag 
      tgctactac agcgaggag agcgtcaac ctggacgtc 
      gaagtcccc aacagcacc acccaggag gagttcctc 
      cacaacctc accctcaac agcttcaaa ttgaacgaa 
      tatcgcgcc aggcacgag gaggagaag tcgaaaaag 
      ttgagcaaa ctcggagtg tccaaaaca agccaaatt 
      agtctggac agcgatccg aaggacagt ttcgtgtcg 
      gacaacgtc gaagttgtt aacaaggac gaagagccc 
      gatagcggc acgctgaag aggaagtcg cgagatagc 
      ggggaggaa caaaaagtc gcgcacatg gttaagagg 
      atgtccgat aataattta tcaaacggg catgtggag 
      tacaagaga cacatgtcg gtccccgct gaaatcacg 
      aagccgagt ccagatggt tcagtattg aaaaaagtc 
      gcctcgctt aatttaaac aaagccgaa cttgaggca 
      aagatttca aagccgagg tttgtgccc gaaaaactg 
      gattttcaa ctgtacgaa aaattcgaa ggtcacatg 
      ttggtgaat tggtacttg tctgagttc agtgaagac 
      caccacttg gttcagttg ctcaacggc caagagttg 
      aagcttctg gcgatccag ttctgcact cacctcctg 
      gccgctggc gttttgcgt caaatccca gacaaagat 
      gtcccaatg tacaacata ttcaaaccc gattgcatg 
      tactactgg gcccatgcg gagactcca atgtccgtc 
      cctcagaca ccgggaagg ctcagcatg gtttcgtgg 
      cctccgaca tcgccatcg gaggctttc ctcacagcg 
      agcataaac gacgccgcc acggcatca tcacctaaa 
      agtcctgaa ctgccattc ggggaaccc gaagagcga 
      cctaggagc aaccaagag ttcaaaaac tcggcccga 
      gacgtcgaa atcctagca ctagaggaa gaaataaat 
      cggttgaag caggaagta gagaacccc actatccca 
      accccgccc ccacctcct cctccccca atgccggga 
      attggagcg cccccacct cctccaatg ccgggaatt 
      ggagcgccc ccacctcct ccaatgccg ggaattgga 
      gcgcaccca ccacctcca atgcccggg attgtcgga 
      cccccaccg ccccccatg ccgggaata gggggccct 
      ccaccgccc ccaatgccg ggcacgggg ccgccgccc 
      cctccgccc ccaatgggc ggtgtgcct ccgcccccg 
      ccgccgatg ggagggcca gtgccgctg ccgccgccc 
      ccagccggg gggtggagt tcgcaaaag gcaggattg 
      cgaaaagcc actttaaat ccgaaagta ccgatgaag 
      cctttgtat tggaccaga atcctggca cctgctgga 
      acagatagt gtcaattct ccgtcatct gatgctctt 
      tggacacaa atagatgaa ttaccttta gatagttta 
      aatgaattc gtggattta ttttcaagg caagttgtt 
      acgagaaaa cctacggtg aagaaacaa gaacagaag 
      gctaaagct gaagctgtc aagcttttg gatagtaaa 
      agatcacaa aatgttgga atccttgct caaagttta 
      cacgtagat ttccaagaa attgaaaat gctatttac 
      aattttgat acttctatc gttagttta gaagctttg 
      caacagatt tacgaagtt agagcaact gcagaagaa 
      ttagaactt attaaaaac catctatca acgaaacct 
      aacatacca ttagacaag cctgaacag tttttacac 
      gaattatcg gaaatatca aatttcgcg gatagaatc 
      gcttgctta atgtttcaa gttgaattc gacgattct 
      atcaatact atagggcat actttaaca aatataaaa 
      actacttgt gattactta gttaatagt aatgaatta 
      aaagaagtt tttgcaata attttaact ctgggaaat 
      tatatgaac ggcggcaat atgacgcgt ggacaagcc 
      gacgggttc ggcttagaa attttacca aaattaaaa 
      gacgttaaa tcgaaagat tccaaagtc acgcttctc 
      cactacatt gtcaaaatc tacatgaaa aaaatcgaa 
      aacccgttt gaacccaat gttgtacta cccgtaccg 
      gaacctgga gatatcgaa cgcgcagct tccgtaaat 
      tttgacgat gtcaaagta aatttacaa aaactggaa 
      aaacaacta acagagtgt gagaaaaaa atccagaaa 
      gtgattgac tcatcaaac cccgacaat cttcaacct 
      ttcaaggat aagatgacc acgttcttg gaacactcg 
      aagaaacag cttgcaaca gaattcgaa aatttggaa 
      gagtgcaaa gtcaagttc atcaagacg atgaagttt 
      tacttgttt aagcccaaa tcggggact ttggaagat 
      tatcccccg aactcattt ttcgaaatg tggctacca 
      ttctgcatc gactttaaa gatatcttc aagaaggaa 
      ctgatcaga atggaaaaa gaaaagatc caagaaatg 
      aggaaacgc gaaaacgag cgagtgtcg gaaccaata 
      aagataaaa gagcgcgaa aacggacta aaatcgaaa 
      gtcaagaaa ctccaagcc aaacacgct tcacaggtg 
      gagaccaat taa
    mRNA sequence
    • >TC012258 RA
      aaaattaca caaatagtg agcgaaaac cggaagttc 
      agttaatta tggaactcg ttacaaaat tgcagtgtt 
      tatttgtga tcccacttt ccagttcga gtgattaca 
      cgtttgtgt ttttgttaa agacattca aaaatattt 
      agagttgtt gtaaattaa gccaatata gaaaaatgg 
      ggaacttgc aaacgagcg agcctaaaa acgccccca 
      aggccggca aagcccaag gtaaagtaa agtctttga 
      taaaaatca ggggaaaaa agggcaaaa tcgactcgg 
      cacaattca ccggagtgg tccctgaag aagccgaag 
      actcagccc tggacgact cgctcccgg tcgcagaaa 
      agccgaaat ccagcaccg acaagatcg tagtgaccg 
      actcctggt gcaaagtca accgcttga ctgaagacg 
      tcgagaaga aaaccaaaa cgccaccga gcgccgtct 
      cctcgagct ccgactcga acttcacgg accctttga 
      caccaatgg gcttcaaca ccgaaatga accagtgct 
      actacagcg aggagagcg tcaacctgg acgtcgaag 
      tccccaaca gcaccaccc aggaggagt tcctccaca 
      acctcaccc tcaacagct tcaaattga acgaatatc 
      gcgccaggc acgaggagg agaagtcga aaaagttga 
      gcaaactcg gagtgtcca aaacaagcc aaattagtc 
      tggacagcg atccgaagg acagtttcg tgtcggaca 
      acgtcgaag ttgttaaca aggacgaag agcccgata 
      gcggcacgc tgaagagga agtcgcgag atagcgggg 
      aggaacaaa aagtcgcgc acatggtta agaggatgt 
      ccgataata atttatcaa acgggcatg tggagtaca 
      agagacaca tgtcggtcc ccgctgaaa tcacgaagc 
      cgagtccag atggttcag tattgaaaa aagtcgcct 
      cgcttaatt taaacaaag ccgaacttg aggcaaaga 
      tttcaaagc cgaggtttg tgcccgaaa aactggatt 
      ttcaactgt acgaaaaat tcgaaggtc acatgttgg 
      tgaattggt acttgtctg agttcagtg aagaccacc 
      acttggttc agttgctca acggccaag agttgaagc 
      ttctggcga tccagttct gcactcacc tcctggccg 
      ctggcgttt tgcgtcaaa tcccagaca aagatgtcc 
      caatgtaca acatattca aacccgatt gcatgtact 
      actgggccc atgcggaga ctccaatgt ccgtccctc 
      agacaccgg gaaggctca gcatggttt cgtggcctc 
      cgacatcgc catcggagg ctttcctca cagcgagca 
      taaacgacg ccgccacgg catcatcac ctaaaagtc 
      ctgaactgc cattcgggg aacccgaag agcgaccta 
      ggagcaacc aagagttca aaaactcgg cccgagacg 
      tcgaaatcc tagcactag aggaagaaa taaatcggt 
      tgaagcagg aagtagaga accccacta tcccaaccc 
      cgcccccac ctcctcctc ccccaatgc cgggaattg 
      gagcgcccc cacctcctc caatgccgg gaattggag 
      cgcccccac ctcctccaa tgccgggaa ttggagcgc 
      acccaccac ctccaatgc ccgggattg tcggacccc 
      caccgcccc ccatgccgg gaatagggg gccctccac 
      cgcccccaa tgccgggca cggggccgc cgccccctc 
      cgcccccaa tgggcggtg tgcctccgc ccccgccgc 
      cgatgggag ggccagtgc cgctgccgc cgcccccag 
      ccggggggt ggagttcgc aaaaggcag tggtccccc 
      ccgttccag tgtccttag gattgcgaa aagccactt 
      taaatccga aagtaccga tgaagcctt tgtattgga 
      ccagaatcc tggcacctg ctggaacag atagtgtca 
      attctccgt catctgatg ctctttgga cacaaatag 
      atgaattac ctttagata gtttaaatg aattcgtgg 
      atttatttt caaggcaag ttgttacga gaaaaccta 
      cggtgaaga aacaagaac agaaggcta aagctgaag 
      ctgtcaagc ttttggata gtaaaagat cacaaaatg 
      ttggaatcc ttgctcaaa gtttacacg tagatttcc 
      aagaaattg aaaatgcta tttacaatt ttgatactt 
      ctatcgtta gtttagaag ctttgcaac agatttacg 
      aagttagag caactgcag aagaattag aacttatta 
      aaaaccatc tatcaacga aacctaaca taccattag 
      acaagcctg aacagtttt tacacgaat tatcggaaa 
      tatcaaatt tcgcggata gaatcgctt gcttaatgt 
      ttcaagttg aattcgacg attctatca atactatag 
      ggcatactt taacaaata taaaaacta cttgtgatt 
      acttagtta atagtaatg aattaaaag aagtttttg 
      caataattt taactctgg gaaattata tgaacggcg 
      gcaatatga cgcgtggac aagccgacg ggttcggct 
      tagaaattt taccaaaat taaaagacg ttaaatcga 
      aagattcca aagtcacgc ttctccact acattgtca 
      aaatctaca tgaaaaaaa tcgaaaacc cgtttgaac 
      ccaatgttg tactacccg taccggaac ctggagata 
      tcgaacgcg cagcttccg taaattttg acgatgtca 
      aagtaaatt tacaaaaac tggaaaaac aactaacag 
      agtgtgaga aaaaaatcc agaaagtga ttgactcat 
      caaaccccg acaatcttc aacctttca aggataaga 
      tgaccacgt tcttggaac actcgaaga aacagcttg 
      caacagaat tcgaaaatt tggaagagt gcaaagtca 
      agttcatca agacgatga agttttact tgtttaagc 
      ccaaatcgg ggactttgg aagattatc ccccgaact 
      catttttcg aaatgtggc taccattct gcatcgact 
      ttaaagata tcttcaaga aggaactga tcagaatgg 
      aaaaagaaa agatccaag aaatgagga aacgcgaaa 
      acgagcgag tgtcggaac caataaaga taaaagagc 
      gcgaaaacg gactaaaat cgaaagtca agaaactcc 
      aagccaaac acgcttcac aggtggaga ccaattaat 
      acttataca aagtttacg ttatgtatt tgttacaaa 
      aataagttt tatttattg ttttacgtc gaatattac 
      ctgtgtt
    • >TC012258 RB
      aaaattaca caaatagtg agcgaaaac cggaagttc 
      agttaatta tggaactcg ttacaaaat tgcagtgtt 
      tatttgtga tcccacttt ccagttcga gtgattaca 
      cgtttgtgt ttttgttaa agacattca aaaatattt 
      agagttgtt gtaaattaa gccaatata gaaaaatgg 
      ggaacttgc aaacgagcg agcctaaaa acgccccca 
      aggccggca aagcccaag gtaaagtaa agtctttga 
      taaaaatca ggggaaaaa agggcaaaa tcgactcgg 
      cacaattca ccggagtgg tccctgaag aagccgaag 
      actcagccc tggacgact cgctcccgg tcgcagaaa 
      agccgaaat ccagcaccg acaagatcg tagtgaccg 
      actcctggt gcaaagtca accgcttga ctgaagacg 
      tcgagaaga aaaccaaaa cgccaccga gcgccgtct 
      cctcgagct ccgactcga acttcacgg accctttga 
      caccaatgg gcttcaaca ccgaaatga accagtgct 
      actacagcg aggagagcg tcaacctgg acgtcgaag 
      tccccaaca gcaccaccc aggaggagt tcctccaca 
      acctcaccc tcaacagct tcaaattga acgaatatc 
      gcgccaggc acgaggagg agaagtcga aaaagttga 
      gcaaactcg gagtgtcca aaacaagcc aaattagtc 
      tggacagcg atccgaagg acagtttcg tgtcggaca 
      acgtcgaag ttgttaaca aggacgaag agcccgata 
      gcggcacgc tgaagagga agtcgcgag atagcgggg 
      aggaacaaa aagtcgcgc acatggtta agaggatgt 
      ccgataata atttatcaa acgggcatg tggagtaca 
      agagacaca tgtcggtcc ccgctgaaa tcacgaagc 
      cgagtccag atggttcag tattgaaaa aagtcgcct 
      cgcttaatt taaacaaag ccgaacttg aggcaaaga 
      tttcaaagc cgaggtttg tgcccgaaa aactggatt 
      ttcaactgt acgaaaaat tcgaaggtc acatgttgg 
      tgaattggt acttgtctg agttcagtg aagaccacc 
      acttggttc agttgctca acggccaag agttgaagc 
      ttctggcga tccagttct gcactcacc tcctggccg 
      ctggcgttt tgcgtcaaa tcccagaca aagatgtcc 
      caatgtaca acatattca aacccgatt gcatgtact 
      actgggccc atgcggaga ctccaatgt ccgtccctc 
      agacaccgg gaaggctca gcatggttt cgtggcctc 
      cgacatcgc catcggagg ctttcctca cagcgagca 
      taaacgacg ccgccacgg catcatcac ctaaaagtc 
      ctgaactgc cattcgggg aacccgaag agcgaccta 
      ggagcaacc aagagttca aaaactcgg cccgagacg 
      tcgaaatcc tagcactag aggaagaaa taaatcggt 
      tgaagcagg aagtagaga accccacta tcccaaccc 
      cgcccccac ctcctcctc ccccaatgc cgggaattg 
      gagcgcccc cacctcctc caatgccgg gaattggag 
      cgcccccac ctcctccaa tgccgggaa ttggagcgc 
      acccaccac ctccaatgc ccgggattg tcggacccc 
      caccgcccc ccatgccgg gaatagggg gccctccac 
      cgcccccaa tgccgggca cggggccgc cgccccctc 
      cgcccccaa tgggcggtg tgcctccgc ccccgccgc 
      cgatgggag ggccagtgc cgctgccgc cgcccccag 
      ccggggggt ggagttcgc aaaaggcag gattgcgaa 
      aagccactt taaatccga aagtaccga tgaagcctt 
      tgtattgga ccagaatcc tggcacctg ctggaacag 
      atagtgtca attctccgt catctgatg ctctttgga 
      cacaaatag atgaattac ctttagata gtttaaatg 
      aattcgtgg atttatttt caaggcaag ttgttacga 
      gaaaaccta cggtgaaga aacaagaac agaaggcta 
      aagctgaag ctgtcaagc ttttggata gtaaaagat 
      cacaaaatg ttggaatcc ttgctcaaa gtttacacg 
      tagatttcc aagaaattg aaaatgcta tttacaatt 
      ttgatactt ctatcgtta gtttagaag ctttgcaac 
      agatttacg aagttagag caactgcag aagaattag 
      aacttatta aaaaccatc tatcaacga aacctaaca 
      taccattag acaagcctg aacagtttt tacacgaat 
      tatcggaaa tatcaaatt tcgcggata gaatcgctt 
      gcttaatgt ttcaagttg aattcgacg attctatca 
      atactatag ggcatactt taacaaata taaaaacta 
      cttgtgatt acttagtta atagtaatg aattaaaag 
      aagtttttg caataattt taactctgg gaaattata 
      tgaacggcg gcaatatga cgcgtggac aagccgacg 
      ggttcggct tagaaattt taccaaaat taaaagacg 
      ttaaatcga aagattcca aagtcacgc ttctccact 
      acattgtca aaatctaca tgaaaaaaa tcgaaaacc 
      cgtttgaac ccaatgttg tactacccg taccggaac 
      ctggagata tcgaacgcg cagcttccg taaattttg 
      acgatgtca aagtaaatt tacaaaaac tggaaaaac 
      aactaacag agtgtgaga aaaaaatcc agaaagtga 
      ttgactcat caaaccccg acaatcttc aacctttca 
      aggataaga tgaccacgt tcttggaac actcgaaga 
      aacagcttg caacagaat tcgaaaatt tggaagagt 
      gcaaagtca agttcatca agacgatga agttttact 
      tgtttaagc ccaaatcgg ggactttgg aagattatc 
      ccccgaact catttttcg aaatgtggc taccattct 
      gcatcgact ttaaagata tcttcaaga aggaactga 
      tcagaatgg aaaaagaaa agatccaag aaatgagga 
      aacgcgaaa acgagcgag tgtcggaac caataaaga 
      taaaagagc gcgaaaacg gactaaaat cgaaagtca 
      agaaactcc aagccaaac acgcttcac aggtggaga 
      ccaattaat acttataca aagtttacg ttatgtatt 
      tgttacaaa aataagttt tatttattg ttttacgtc 
      gaatattac ctgtgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY