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Gene information
General information
TC001850 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450423.1: 4529..9573
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001850
    iBeetle screen iB_06403

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001850 RA
      MEDGLNGIS SESEAPNHV NNGVASPIM ANFSEPEEA 
      VSKEDTVSK VNGDSEPVS NPEEYSSES DALVIDDKK 
      PASPESELI PHFNPYFNE SIFELSQQH QNDSSLKPS 
      APQVGLHKH KRLKAKNSP KLREYAQYL GLQPTVQFK 
      CSRCGRAGF ESLTTLNEH IIQCTDEPS AEPQAPENS 
      SSSFKLRRK VFLCSACGT YYENWNLYL HMLEVHRRY 
      LCLYCLGMF SVLDDLCLH IQSRHNLEP GRKDTLDDF 
      FSAYSDPCY IVCCECNKL FNEQDNFFY HNCAKKSQK 
      TNKPQKPKQ QIRPEDHVT GETLINGNC SQEPEKQKT 
      PASPETQTD NQIDDTVIE KQPEEIVYS TDDELDFKG 
      FTEAGEPVY KNDKVTPEN PEPEDPPDE AMEVEEEPI 
      ETRKVPKLS LKLKPGSFP QIEQHEPEN PEPEIEQIE 
      SEEIAPSVE EEPPSEPKE EEPPEMKIA GPEVNIIEL 
      ELEQPLDKF DIRILLQKC LNATAPFCI YCNHARKIA 
      VNGKQLGLH AIAEHRFSA VVSSITAEE LIPESFVAR 
      IKESLPELE TVFFSLESS PSDEGVTFS HVFECFQCR 
      YSTTVHKEL YLHNRKSHA KNLLLCIMC KSNFYSYSE 
      LICHLCPGV YILDYDVGF RCCMCVGDD LPSAFRLMV 
      HLRKRHHVC DVCLEMCHS QYRLSNHVW KHKLHHFCY 
      RCGIAYRNK PDITRHLFW KHGTESMLC KKCLQKKWP 
      HVYHFCVPP TSFTCEECN LTFTRAVSL KVHKRIHTD 
      EKPHACTID DCQERFISK KLLAKHELK HKEPPEEPK 
      VEEKEESKE NGENKEETE ESKEEKVEE KEKVKPKVD 
      VYDLPELNL SESDSSESE DEAEKAKKE EETKEVVEE 
      NQIENEILE PAEVVEDPV LENNEVVLE NIWDDFKNY 
      QANKEKFDH MFIDSEKSE PSQAEPEPV IENEPLTLE 
      MALRDHDYC IDPDNKPDI SFTEVKPDL SQSVDHDYC 
      TAKKDSAKP ETTEEKPVP ETKPEKKAN SSSSDSSSD 
      SDSSSCSCG PNCSCSSSS GSSSSSSES SDSDSSTEE 
      GRKRQQLRR DRRRQRKNK GKDNETKDI VVDNTVPMA 
      DMPILESDL ETTESETDE EFYDKQPHL IAKKILEEK 
      RNQILAGID QAPNGSLLE NSRAATPNL PEEEQVKKK 
      KSKSKKKKK KKSERKEKY NASNIPEYY YQPPTVAPI 
      TLTLSRNSP VSSPSAKLP PSNIYPKSE AELPREESD 
      TSLRASKRR RVPNKFYGY SSDEEEAPK WRKTEVFPV 
      QPPLTVPPI TIKTGPPPV QPFRGYPNK DTPRKMPTI 
      KLKPKVEDG ESEVESNDS SNEGRNMLQ VNPDALYCY 
      CKCPYDEVS EMIGCDSRD CSIEWFHFE CVGIMVPPK 
      GHWYCPECR KKRQQKREA LKLKAKTS
    CDS sequence
    • >TC001850 RA
      atggaagat ggtttgaac ggcatttca tccgagagc 
      gaggcccca aatcatgtg aataatggc gtagcctct 
      ccgataatg gcaaatttc tctgagcca gaggaggcc 
      gtttcgaag gaagacacc gtcagcaaa gtaaacgga 
      gacagtgag ccggtttcc aacccggag gagtacagc 
      tcggagagc gacgccctg gtgatcgac gacaaaaag 
      ccagcctcg ccggaatcg gagctcatc ccccacttc 
      aacccctac ttcaacgag tcgatcttc gagctgtcc 
      cagcagcac cagaacgac agctcgctc aagcccagc 
      gccccgcaa gttggcctc cacaagcac aagcggctg 
      aaggcgaag aacagcccc aagttgcgc gagtacgcc 
      cagtacctg gggctccag cccacagtg cagttcaag 
      tgctcgcga tgcggacgc gccggtttc gagtcgctg 
      acgacgctc aacgagcac atcatccag tgcaccgac 
      gagcccagt gcggagccc caagccccc gagaactcc 
      tcctctagc ttcaagctg cggcggaaa gtgttcctg 
      tgctccgcc tgtggaacg tactacgag aactggaac 
      ttgtacttg cacatgctg gaagtgcac agacgctac 
      ttgtgttta tattgtttg ggtatgttt tcagtatta 
      gacgatttg tgcttacac atccaatcg cgacacaac 
      ctcgagccc ggccgcaaa gacaccctc gacgacttt 
      tttagtgcg tattcggac ccgtgctac atcgtgtgc 
      tgtgaatgc aacaagctc ttcaacgaa caagacaat 
      tttttctac cacaattgc gccaagaaa agccaaaaa 
      accaacaaa ccgcaaaaa cccaaacag cagatccgg 
      cccgaagat cacgtcacc ggcgaaacc ctcatcaac 
      ggcaattgt tcgcaagaa cccgaaaaa caaaagacc 
      cccgcttcc ccggaaaca cagactgac aaccaaatt 
      gatgacacc gtcatcgaa aaacagccg gaagaaatc 
      gtctactca accgacgac gagctggat ttcaaagga 
      ttcaccgag gccggagag cccgtctac aagaacgac 
      aaagtgacc cccgaaaac cccgagcca gaggacccc 
      ccggacgag gccatggag gtcgaagaa gagccaatc 
      gaaacgcgc aaagttcct aaactctca ctcaagctc 
      aaacccggg tctttcccc caaatcgag caacacgag 
      ccggagaac cccgaaccc gaaatcgag caaatcgaa 
      agcgaggag atcgccccc agcgtggag gaggagccc 
      cccagtgag cccaaagaa gaggagcca ccggaaatg 
      aaaattgcc ggtcccgaa gtcaacata atcgagctc 
      gagctggaa cagcccttg gacaagttc gatattcgc 
      attttgttg cagaaatgt ctcaacgcc actgccccc 
      ttctgcatc tactgcaac cacgcgcgc aagatcgca 
      gtcaacggc aaacaactc ggactgcac gcgattgcc 
      gagcaccgc ttctcagct gtcgtgagt tccataacc 
      gccgaggag ctcattccc gaaagtttc gttgcgcga 
      attaaggag agccttcct gagttggaa acagtgttt 
      ttcagtctt gaatctagt ccctcggac gaaggcgtc 
      actttctcc cacgttttt gagtgtttc cagtgccgg 
      tactccaca acagttcac aaggagctc tacctacac 
      aaccggaag tcccacgcg aaaaacctc ctcttgtgc 
      atcatgtgc aagtcgaat ttttacagt tatagcgaa 
      ttgatttgt cacttgtgt cccggagtt tacattctt 
      gactacgat gtcggtttc cggtgttgt atgtgtgtt 
      ggggacgac ctcccgtcg gcttttcgc ctcatggtg 
      cacctccgg aaacgacac cacgtctgc gatgtgtgt 
      ctagaaatg tgccatagt cagtatcgg ttgtcaaac 
      cacgtctgg aagcataaa ttacaccac ttttgctac 
      cgctgcggt atagcttat aggaacaaa cccgacatc 
      acgagacac ctgttctgg aagcacggc accgaaagc 
      atgctttgt aaaaaatgc ctgcagaag aagtggccc 
      cacgtttac cacttctgc gtaccgccg actagcttt 
      acgtgcgag gagtgcaat ctaacgttc acacgagct 
      gtttccttg aaagtacac aaaaggata catacggat 
      gagaagcca cacgcttgc accattgac gattgccaa 
      gagaggttc atttcgaag aaactgcta gcgaagcac 
      gagctgaaa cacaaggaa ccgcctgaa gagcccaaa 
      gtcgaggag aaagaagag agtaaagaa aacggcgaa 
      aataaggaa gaaactgaa gagagtaaa gaggaaaag 
      gtggaggag aaagagaaa gttaagccg aaagtcgac 
      gtttacgac ttgcctgaa ctgaacctc tccgaaagt 
      gacagtagt gagtcggag gacgaggcg gaaaaagcg 
      aagaaagag gaagaaacc aaggaagtg gttgaagaa 
      aatcagatt gagaacgag atccttgaa ccggccgaa 
      gtggtcgag gacccagtc ctggaaaac aatgaggtc 
      gttctggag aacatttgg gacgatttt aaaaattat 
      caagcgaat aaagaaaag ttcgaccac atgttcatc 
      gatagcgag aagagtgag ccaagtcag gccgagcct 
      gagccagtt atcgaaaac gaacctttg actcttgaa 
      atggccctg cgggatcat gactattgc atagacccc 
      gataacaag cccgatatc agtttcact gaagtgaaa 
      cccgacctg tcacaaagc gtggaccat gactattgc 
      accgccaag aaagactca gccaagcct gaaacgacc 
      gaggaaaaa ccagtgccg gaaaccaaa ccggagaag 
      aaggccaat agctcatcg agcgattcg agcagcgac 
      agcgactcg tcgagctgt tcctgtggc ccgaactgt 
      tcctgcagc tcaagttcg ggctcttcc agttcgagt 
      tcggaaagt tccgactcg gattcgtcg acggaagag 
      ggccgtaag cgccaacaa ttgcgacgt gaccgccgc 
      cggcagcga aaaaataaa ggaaaagac aatgaaacc 
      aaagatatt gtcgtcgat aatacagtc cccatggcc 
      gatatgccc atccttgag tccgacttg gaaactacc 
      gaaagcgaa acggacgaa gagttctac gacaagcag 
      ccacacctg atcgctaag aagatccta gaagagaaa 
      cgcaaccaa atcctcgcg gggatcgac caagcaccg 
      aacggttcc ctgctcgag aacagtcgg gcagcgacc 
      ccgaacctc cccgaggag gagcaagtc aagaagaag 
      aaatccaag tcgaagaaa aagaaaaag aagaagagc 
      gaacgcaaa gaaaagtat aacgcgtcc aacattcca 
      gagtattac taccaaccg ccgaccgtg gccccaatc 
      accctcacc ctgtcgcgg aactcgccg gtgtcctcc 
      cccagcgcg aaactcccc ccgagtaac atttacccc 
      aagagcgag gccgagttg ccgcgcgag gaaagcgat 
      acgagttta agagcctcg aagcggagg cgcgttcct 
      aataaattc tacggttat tcgagcgac gaagaggag 
      gctccgaag tggcgcaag acggaggtt ttcccagtg 
      cagccccca ctgactgtg cccccgatc acgataaag 
      acagggccg ccaccggtg cagcccttc cgggggtac 
      ccgaacaag gacactccg cggaaaatg cccacgatc 
      aagttgaag ccgaaggtg gaggatggg gagagcgag 
      gtggagtcg aacgactcg agtaacgag gggcggaac 
      atgctccag gtgaacccg gacgcgctg tactgctac 
      tgcaagtgt ccctatgat gaggtttcg gagatgata 
      gggtgcgat tcgagggat tgcagcatc gaatggttc 
      catttcgag tgtgtgggg atcatggtg ccgcccaag 
      gggcactgg tattgtcca gagtgtagg aaaaaaaga 
      caacagaaa cgagaagcg ctcaaattg aaggcgaaa 
      acttcgtga
    mRNA sequence
    • >TC001850 RA
      cgaaatgga gcgccattt tattaacct tttaggcgg 
      ggagacgta tttctcgat aaatatgtg ctcaaatgt 
      gctaaataa aaccgttcc cgtcgatat tataaaccg 
      ttaattcca cctgatcgt acgcctcgt agagtagaa 
      tcggcatgg aagatggtt tgaacggca tttcatccg 
      agagcgagg ccccaaatc atgtgaata atggcgtag 
      cctctccga taatggcaa atttctctg agccagagg 
      aggccgttt cgaaggaag acaccgtca gcaaagtaa 
      acggagaca gtgagccgg tttccaacc cggaggagt 
      acagctcgg agagcgacg ccctggtga tcgacgaca 
      aaaagccag cctcgccgg aatcggagc tcatccccc 
      acttcaacc cctacttca acgagtcga tcttcgagc 
      tgtcccagc agcaccaga acgacagct cgctcaagc 
      ccagcgccc cgcaagttg gcctccaca agcacaagc 
      ggctgaagg cgaagaaca gccccaagt tgcgcgagt 
      acgcccagt acctggggc tccagccca cagtgcagt 
      tcaagtgct cgcgatgcg gacgcgccg gtttcgagt 
      cgctgacga cgctcaacg agcacatca tccagtgca 
      ccgacgagc ccagtgcgg agccccaag cccccgaga 
      actcctcct ctagcttca agctgcggc ggaaagtgt 
      tcctgtgct ccgcctgtg gaacgtact acgagaact 
      ggaacttgt acttgcaca tgctggaag tgcacagac 
      gctacttgt gtttatatt gtttgggta tgttttcag 
      tattagacg atttgtgct tacacatcc aatcgcgac 
      acaacctcg agcccggcc gcaaagaca ccctcgacg 
      actttttta gtgcgtatt cggacccgt gctacatcg 
      tgtgctgtg aatgcaaca agctcttca acgaacaag 
      acaattttt tctaccaca attgcgcca agaaaagcc 
      aaaaaacca acaaaccgc aaaaaccca aacagcaga 
      tccggcccg aagatcacg tcaccggcg aaaccctca 
      tcaacggca attgttcgc aagaacccg aaaaacaaa 
      agacccccg cttccccgg aaacacaga ctgacaacc 
      aaattgatg acaccgtca tcgaaaaac agccggaag 
      aaatcgtct actcaaccg acgacgagc tggatttca 
      aaggattca ccgaggccg gagagcccg tctacaaga 
      acgacaaag tgacccccg aaaaccccg agccagagg 
      accccccgg acgaggcca tggaggtcg aagaagagc 
      caatcgaaa cgcgcaaag ttcctaaac tctcactca 
      agctcaaac ccgggtctt tcccccaaa tcgagcaac 
      acgagccgg agaaccccg aacccgaaa tcgagcaaa 
      tcgaaagcg aggagatcg cccccagcg tggaggagg 
      agcccccca gtgagccca aagaagagg agccaccgg 
      aaatgaaaa ttgccggtc ccgaagtca acataatcg 
      agctcgagc tggaacagc ccttggaca agttcgata 
      ttcgcattt tgttgcaga aatgtctca acgccactg 
      cccccttct gcatctact gcaaccacg cgcgcaaga 
      tcgcagtca acggcaaac aactcggac tgcacgcga 
      ttgccgagc accgcttct cagctgtcg tgagttcca 
      taaccgccg aggagctca ttcccgaaa gtttcgttg 
      cgcgaatta aggagagcc ttcctgagt tggaaacag 
      tgtttttca gtcttgaat ctagtccct cggacgaag 
      gcgtcactt tctcccacg tttttgagt gtttccagt 
      gccggtact ccacaacag ttcacaagg agctctacc 
      tacacaacc ggaagtccc acgcgaaaa acctcctct 
      tgtgcatca tgtgcaagt cgaattttt acagttata 
      gcgaattga tttgtcact tgtgtcccg gagtttaca 
      ttcttgact acgatgtcg gtttccggt gttgtatgt 
      gtgttgggg acgacctcc cgtcggctt ttcgcctca 
      tggtgcacc tccggaaac gacaccacg tctgcgatg 
      tgtgtctag aaatgtgcc atagtcagt atcggttgt 
      caaaccacg tctggaagc ataaattac accactttt 
      gctaccgct gcggtatag cttatagga acaaacccg 
      acatcacga gacacctgt tctggaagc acggcaccg 
      aaagcatgc tttgtaaaa aatgcctgc agaagaagt 
      ggccccacg tttaccact tctgcgtac cgccgacta 
      gctttacgt gcgaggagt gcaatctaa cgttcacac 
      gagctgttt ccttgaaag tacacaaaa ggatacata 
      cggatgaga agccacacg cttgcacca ttgacgatt 
      gccaagaga ggttcattt cgaagaaac tgctagcga 
      agcacgagc tgaaacaca aggaaccgc ctgaagagc 
      ccaaagtcg aggagaaag aagagagta aagaaaacg 
      gcgaaaata aggaagaaa ctgaagaga gtaaagagg 
      aaaaggtgg aggagaaag agaaagtta agccgaaag 
      tcgacgttt acgacttgc ctgaactga acctctccg 
      aaagtgaca gtagtgagt cggaggacg aggcggaaa 
      aagcgaaga aagaggaag aaaccaagg aagtggttg 
      aagaaaatc agattgaga acgagatcc ttgaaccgg 
      ccgaagtgg tcgaggacc cagtcctgg aaaacaatg 
      aggtcgttc tggagaaca tttgggacg attttaaaa 
      attatcaag cgaataaag aaaagttcg accacatgt 
      tcatcgata gcgagaaga gtgagccaa gtcaggccg 
      agcctgagc cagttatcg aaaacgaac ctttgactc 
      ttgaaatgg ccctgcggg atcatgact attgcatag 
      accccgata acaagcccg atatcagtt tcactgaag 
      tgaaacccg acctgtcac aaagcgtgg accatgact 
      attgcaccg ccaagaaag actcagcca agcctgaaa 
      cgaccgagg aaaaaccag tgccggaaa ccaaaccgg 
      agaagaagg ccaatagct catcgagcg attcgagca 
      gcgacagcg actcgtcga gctgttcct gtggcccga 
      actgttcct gcagctcaa gttcgggct cttccagtt 
      cgagttcgg aaagttccg actcggatt cgtcgacgg 
      aagagggcc gtaagcgcc aacaattgc gacgtgacc 
      gccgccggc agcgaaaaa ataaaggaa aagacaatg 
      aaaccaaag atattgtcg tcgataata cagtcccca 
      tggccgata tgcccatcc ttgagtccg acttggaaa 
      ctaccgaaa gcgaaacgg acgaagagt tctacgaca 
      agcagccac acctgatcg ctaagaaga tcctagaag 
      agaaacgca accaaatcc tcgcgggga tcgaccaag 
      caccgaacg gttccctgc tcgagaaca gtcgggcag 
      cgaccccga acctccccg aggaggagc aagtcaaga 
      agaagaaat ccaagtcga agaaaaaga aaaagaaga 
      agagcgaac gcaaagaaa agtataacg cgtccaaca 
      ttccagagt attactacc aaccgccga ccgtggccc 
      caatcaccc tcaccctgt cgcggaact cgccggtgt 
      cctccccca gcgcgaaac tccccccga gtaacattt 
      accccaaga gcgaggccg agttgccgc gcgaggaaa 
      gcgatacga gtttaagag cctcgaagc ggaggcgcg 
      ttcctaata aattctacg gttattcga gcgacgaag 
      aggaggctc cgaagtggc gcaagacgg aggttttcc 
      cagtgcagc ccccactga ctgtgcccc cgatcacga 
      taaagacag ggccgccac cggtgcagc ccttccggg 
      ggtacccga acaaggaca ctccgcgga aaatgccca 
      cgatcaagt tgaagccga aggtggagg atggggaga 
      gcgaggtgg agtcgaacg actcgagta acgaggggc 
      ggaacatgc tccaggtga acccggacg cgctgtact 
      gctactgca agtgtccct atgatgagg tttcggaga 
      tgatagggt gcgattcga gggattgca gcatcgaat 
      ggttccatt tcgagtgtg tggggatca tggtgccgc 
      ccaaggggc actggtatt gtccagagt gtaggaaaa 
      aaagacaac agaaacgag aagcgctca aattgaagg 
      cgaaaactt cgtgaagtg gggtttttg ggtttactg 
      tttagaagc ttgtggttt tgatattat ttaaaagta 
      cgcttataa gttgttaat ttttgtaaa gttttacca 
      cccagtaaa ttattttcc ttctgatgt ggccgttct 
      tggtgatta tatttttat gtacacagc ttttaactg 
      gtaaatttt taggtacta aaggttgta cataattgt 
      tctgtacct gtacatagt tacattgtg attgaagga 
      aatcattta aattaatgt attttgtag aattacctt 
      ttttattat taagtagtt ttattttct cttgtcgtg 
      tttaatata gaactttgc caaattaga tatgtaaat 
      ctgtaaata tttttatta atatcggct ctctttaca 
      gagaacttt tccactgta tggaaaata tgttatcct 
      aagatctga cttgatttc agagttaaa tttgatgtt 
      tgtaacttt ctgatagac agaataaac atcgggaag 
      taat
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