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Gene information
General information
TC006818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14568245..14569511
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006818
    iBeetle screen iB_06401

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006818 RA
      MLSQEEKNM YFSSLFGGA VGGLVVDVV LFPLDTLKT 
      RLQSGVGFR KAGGFSGIY KGIGPQAIG SAPQAAFFF 
      LTYESFKYY TEPHVAPHS LPLVYMTGA SISEVVACL 
      IRVPMEVVK QRRQTTTNH KHTSLRILK HAIKSEGII 
      KGLYRGFGS TIIREIPFS LIQFPVLEY LKSTYRINF 
      KNNIPLESW EVANCGAIA GGFAAAVTT PLDVAKTRI 
      MLADKKTAT KMRVRSVLS QIYYEQGIK GLFAGFAPR 
      VLWITLGGY VFFGMYDFS KNFCNEYFL DSEFENR
    CDS sequence
    • >TC006818 RA
      atgttaagt caggaagaa aaaaacatg tatttttca 
      tcattgttt ggtggcgca gttggtggt ttggtggtc 
      gatgttgtt ttatttcct ctcgatacg cttaagaca 
      cgtcttcag tcaggcgta ggttttagg aaagcgggt 
      ggattttca gggatttat aaaggtata gggccccaa 
      gcaatcgga agtgcccca caagcggca ttttttttt 
      cttacttat gaaagtttt aaatattat actgagcct 
      catgttgcg ccacattcg ttgcctctt gtttacatg 
      accggtgcc tcaatcagt gaagttgtc gcttgcctc 
      atccgagtc cctatggaa gtggtgaaa caaaggcgt 
      cagaccact acgaatcac aaacacaca tctttgcgt 
      attttaaaa cacgcgatt aaatctgag gggatcata 
      aaagggtta tatcgaggg tttggctca acgataatt 
      cgagagata cctttttcg ttgattcag ttcccggtt 
      cttgaatat ttaaaatcg acttatagg ataaatttt 
      aagaataat attccttta gaatcttgg gaggttgcg 
      aattgtgga gctatcgca ggaggcttt gcggctgct 
      gtgaccaca cctttagac gtagccaaa acacgaatt 
      atgttagct gataagaaa acagcaacc aaaatgcga 
      gtcagaagt gtattgagc caaatttat tacgaacaa 
      ggaattaag ggacttttt gcgggattt gcaccacgg 
      gttttatgg ataacactt ggagggtac gtgtttttt 
      ggaatgtac gatttttca aagaacttt tgcaatgag 
      tatttcttg gattctgag tttgaaaat agatga
    mRNA sequence
    • >TC006818 RA
      tgttatcgc attaacgaa atgggccaa aggttgtgt 
      taggtgttt ttgaagttt tgttgtggg gacttgaaa 
      ggtgaataa caaccgatt tttgactta atttaaaca 
      aaatcaaaa gtttggttg attttattt cgcaacaat 
      gttaagtca ggaagaaaa aaacatgta tttttcatc 
      attgtttgg tggcgcagt tggtggttt ggtggtcga 
      tgttgtttt atttcctct cgatacgct taagacacg 
      tcttcagtc aggcgtagg ttttaggaa agcgggtgg 
      attttcagg gatttataa aggtatagg gccccaagc 
      aatcggaag tgccccaca agcggcatt tttttttct 
      tacttatga aagttttaa atattatac tgagcctca 
      tgttgcgcc acattcgtt gcctcttgt ttacatgac 
      cggtgcctc aatcagtga agttgtcgc ttgcctcat 
      ccgagtccc tatggaagt ggtgaaaca aaggcgtca 
      gaccactac gaatcacaa acacacatc tttgcgtat 
      tttaaaaca cgcgattaa atctgaggg gatcataaa 
      agggttata tcgagggtt tggctcaac gataattcg 
      agagatacc tttttcgtt gattcagtt cccggttct 
      tgaatattt aaaatcgac ttataggat aaattttaa 
      gaataatat tcctttaga atcttggga ggttgcgaa 
      ttgtggagc tatcgcagg aggctttgc ggctgctgt 
      gaccacacc tttagacgt agccaaaac acgaattat 
      gttagctga taagaaaac agcaaccaa aatgcgagt 
      cagaagtgt attgagcca aatttatta cgaacaagg 
      aattaaggg actttttgc gggatttgc accacgggt 
      tttatggat aacacttgg agggtacgt gttttttgg 
      aatgtacga tttttcaaa gaacttttg caatgagta 
      tttcttgga ttctgagtt tgaaaatag atgatttta 
      gtcaaatgg gttattaaa cataagtta tttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY