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Gene information
General information
TC031293
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006815
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14560119..14561990
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031293
    iBeetle screen iB_06400

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    elytra size shortened - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031293 RA
      MVKNILVFV PNSLSLNKS GYLEGFRQD YDESEAYYI 
      TCDVTVKQT RNVIGFLGN DVITKMKNV DFKAVHINT 
      NSKISLLNF GNLANSSVV RIVYDFGAF RDCDDLDDD 
      QYGIHFKFL SSHLRKHKR KNEENGREN ALTFVIAIL 
      GWLLQFLNK FKLVWAYSA TISHFSDNL NAFKWFFAE 
      VNKEKRVSP RIGNFILAK LLDLILGII LLRIFIENE 
      NLIVNFIDN IRETLILNF RHLLIYLMG SPIGLKLNY 
      AFNKSLGRF FFYHISLWR VFLLTMQPF VKQYFKLLV 
      LPGAFGFSY QIAMLSDLI SIATFHVYC IYVYAARLI 
      YLQLKGLLS LWRLFIGRK YNPLRARVD SYQYSQHQL 
      FIGTLGFTV LLFLLPTTT MYYTVFVIF RLAIMIVEE 
      ILLRFRYFL QCFPIYIFI LWMFKSPKM SGPVFLKCK 
      KSKDGIVTL EAHLKMLPL LTSLQTFAP NTNKSRTSL 
      SWSKIFTGV LL
    CDS sequence
    • >TC031293 RA
      atggtgaaa aatatcctc gtttttgta ccaaattca 
      ctcagtttg aataagagt ggatatttg gagggcttc 
      cggcaagat tacgatgaa agtgaagct tattacatt 
      acttgtgat gtcacagtt aaacaaaca agaaatgtt 
      atcggtttt ttgggcaac gacgtgatc accaaaatg 
      aaaaatgtt gactttaag gccgttcat attaatacg 
      aactcaaaa attagttta ttaaatttc ggaaatctg 
      gctaattcg agtgtggtg agaattgtt tacgatttt 
      ggagcgttt cgtgattgt gatgatttg gacgatgat 
      cagtatggg atacacttt aagtttctg agcagtcat 
      ttgagaaaa cataagcgg aaaaatgaa gaaaacggg 
      cgcgagaat gccctcact tttgttatt gccatcctg 
      gggtggtta ttacaattt ttaaataaa tttaaatta 
      gtgtgggct tattcagct acgatttca cacttttct 
      gataattta aacgcgttt aagtggttt tttgctgaa 
      gtgaataaa gaaaaacgg gtgtcaccc agaattggg 
      aattttatt ttagcaaaa ttgcttgat ttaatattg 
      gggataatt ttgttacgc atttttata gaaaatgaa 
      aatttgatc gtaaatttc attgataat attagagag 
      actttaatt cttaatttt cgtcacctt ttaatctat 
      ctcatgggg tcccctatc ggtttaaaa ttaaattat 
      gctttcaat aaatcatta ggaagattt ttcttttac 
      cacatttcg ttatggaga gtgttttta ttaacaatg 
      caacctttt gttaaacaa tatttcaaa ttgctagtc 
      ttgcctggc gcttttgga ttttcttat caaatagca 
      atgctttcg gatcttatt tcaattgca acatttcat 
      gtttattgc atttatgtg tatgccgct agactaatt 
      tatttgcaa ctgaaaggt ttactatca ctgtggcgt 
      ctctttatc gggcgaaaa tataaccca ttacgggcc 
      agagttgac tcttatcag tattcgcaa caccaatta 
      tttatcggg actttgggc ttcacagta ttgttattt 
      ctactacct accacaacc atgtattac accgttttt 
      gtgatattt aggttggca ataatgatc gttgaggaa 
      attctacta cgatttcgg tatttctta cagtgtttt 
      cccatttat atttttatt ttatggatg ttcaagtcg 
      ccaaaaatg tcaggtcct gtcttttta aaatgtaaa 
      aaatctaag gatggaatt gtaacactt gaggctcat 
      ttgaaaatg ttgcccttg ttgaccagt ttgcagact 
      ttcgctcca aacacgaac aagtcaagg acgagttta 
      tcttggagt aaaattttc acaggcgta ttactttaa
    mRNA sequence
    • >TC031293 RA
      tgtgctgtc aaaattata tgttaactt gaaccaatt 
      accgaccca aggtcacag atgaggtgc cttaatatt 
      ctatggcct taatctgtg ccgagtttg catcacgcg 
      cacaaacat aaacatttt gacacaaaa atttgaggt 
      tagtcaacg atggtgaaa aatatcctc gtttttgta 
      ccaaattca ctcagtttg aataagagt ggatatttg 
      gagggcttc cggcaagat tacgatgaa agtgaagct 
      tattacatt acttgtgat gtcacagtt aaacaaaca 
      agaaatgtt atcggtttt ttgggcaac gacgtgatc 
      accaaaatg aaaaatgtt gactttaag gccgttcat 
      attaatacg aactcaaaa attagttta ttaaatttc 
      ggaaatctg gctaattcg agtgtggtg agaattgtt 
      tacgatttt ggagcgttt cgtgattgt gatgatttg 
      gacgatgat cagtatggg atacacttt aagtttctg 
      agcagtcat ttgagaaaa cataagcgg aaaaatgaa 
      gaaaacggg cgcgagaat gccctcact tttgttatt 
      gccatcctg gggtggtta ttacaattt ttaaataaa 
      tttaaatta gtgtgggct tattcagct acgatttca 
      cacttttct gataattta aacgcgttt aagtggttt 
      tttgctgaa gtgaataaa gaaaaacgg gtgtcaccc 
      agaattggg aattttatt ttagcaaaa ttgcttgat 
      ttaatattg gggataatt ttgttacgc atttttata 
      gaaaatgaa aatttgatc gtaaatttc attgataat 
      attagagag actttaatt cttaatttt cgtcacctt 
      ttaatctat ctcatgggg tcccctatc ggtttaaaa 
      ttaaattat gctttcaat aaatcatta ggaagattt 
      ttcttttac cacatttcg ttatggaga gtgttttta 
      ttaacaatg caacctttt gttaaacaa tatttcaaa 
      ttgctagtc ttgcctggc gcttttgga ttttcttat 
      caaatagca atgctttcg gatcttatt tcaattgca 
      acatttcat gtttattgc atttatgtg tatgccgct 
      agactaatt tatttgcaa ctgaaaggt ttactatca 
      ctgtggcgt ctctttatc gggcgaaaa tataaccca 
      ttacgggcc agagttgac tcttatcag tattcgcaa 
      caccaatta tttatcggg actttgggc ttcacagta 
      ttgttattt ctactacct accacaacc atgtattac 
      accgttttt gtgatattt aggttggca ataatgatc 
      gttgaggaa attctacta cgatttcgg tatttctta 
      cagtgtttt cccatttat atttttatt ttatggatg 
      ttcaagtcg ccaaaaatg tcaggtcct gtcttttta 
      aaatgtaaa aaatctaag gatggaatt gtaacactt 
      gaggctcat ttgaaaatg ttgcccttg ttgaccagt 
      ttgcagact ttcgctcca aacacgaac aagtcaagg 
      acgagttta tcttggagt aaaattttc acaggcgta 
      ttactttaa ttgatttat ttgtttctt gttatacag 
      taataaata tttgcacct taaataatg gaatataca 
      ttaaaaata taaaatttg tacctaatt attagtgat 
      tgaataaat taacaagta ataaaacgc aaaacccgt 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY