Status
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Gene information
General information
TC005315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14530266..14533353
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005315
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06398

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber early potentiallynot present - Number of animals showing the phenotype: 4

    previtellogenic egg chamber late potentiallynot present - Number of animals showing the phenotype: 4
    egg chamber partially not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    mature egg not present - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005315 RA
      MSCNCATPR GIEVKNSDT QSEVSESGT ATSGFNSNW 
      GSREELRDF VAKHGLKDI SDLYQDRFR VDRKKLEQM 
      LSGDNDALI PADVFFANI MEDTDTYIT WPNKLKLGA 
      KSKKDPHVR IAGKEEDVK AAKDRIMTV LYTRCNRVT 
      MKMDISYTD HSHIIGRGG LSIKRVMEE TQCHVHFPD 
      SNRSNPMEK SNQVSISGD LEGVENARS RVRELIPLI 
      FGFEPPIMA QNIDNTCPY VIKIQEQYK VQVMFKTRS 
      KLHATIVLV KGVEWEVEQ VKQATILLM EYMCENLTN 
      QIPVQMTIQ ISPQHHAIV LGKNHSNLK AIMHYTKCQ 
      IMFPDAQDQ NIPSLKRSN VNISGNIHS VYTARQLLI 
      GSLPLLIIF DLPEDIANL KVRSEQIDE IQNTCDVTI 
      TVRQKSKQM IKACIIKGI ERQASNIYK ARNLILGID 
      DPPIQADIP RLYHIPSTP SPNNDLLTT PTNLNPSPL 
      TPIFTWHFS SDQTTPFEI VQKPQMLYN NNNNKMQQS 
      SNSSGFQSF EQVKADTSL LSSISSNSS NYVSSPPTV 
      FSPQSDNNI PTTSDYQSM NEKKSASFD YDCSKRLAG 
      FKAMQGRPN PDVYRVPTA MWAGYGISH TSPAGMLKK 
      EDDIWKPAE VFPSTSGLH NTSNILDHT PTHFINRIA 
      NTTWSDLPS LLSALGLDR YISLFIRHE VDLPTFTTL 
      SDKDLMTIG VTAFGSRRK MLLAISELN KRSCTFSAA 
      PGAERKSSS SNSPPSHDN SPRQSW
    CDS sequence
    • >TC005315 RA
      atgtcttgt aattgtgct acacctcgt ggaatcgag 
      gtaaaaaat tccgacaca caatcagaa gtgtcggaa 
      agcggcacg gctacttct ggtttcaac tcaaattgg 
      ggctccaga gaggaatta agggatttt gtcgccaaa 
      cacggatta aaggacatt tcggatttg taccaggac 
      cggttccgg gtcgaccgc aaaaagttg gaacagatg 
      cttagtggg gacaacgat gcactgatc cctgctgac 
      gttttcttt gcgaacatt atggaagac actgacact 
      tacataacg tggccaaac aaactcaaa ttgggggct 
      aaatcgaaa aaggatcct cacgtgagg atcgccggc 
      aaagaagag gatgttaaa gccgccaaa gacaggatt 
      atgacggtt ttgtacacg agatgcaat cgtgtaacc 
      atgaaaatg gacataagt tacacggac cattcgcat 
      attatcggc agaggaggg ctgagtatt aaacgtgtc 
      atggaagaa actcaatgc cacgtacat ttccctgat 
      tcgaatcgt tcgaatccg atggaaaaa agcaatcaa 
      gtttcgatt tcgggcgat ttggaaggg gttgaaaat 
      gcaaggagt cgcgttagg gaattaatt ccgctgatt 
      tttgggttt gagcctcca ataatggcc caaaatatt 
      gataatacg tgtccttac gtaattaaa atccaggaa 
      cagtacaaa gtgcaagtc atgttcaaa actaggtcc 
      aaacttcat gctactata gtactagtc aagggagtc 
      gaatgggag gtggagcag gtgaagcag gctacaatt 
      ttgctcatg gaatacatg tgcgaaaat ttgacgaac 
      caaattccg gttcaaatg actatccaa atttcgccc 
      caacaccat gcaatcgtt ttgggcaaa aatcattca 
      aacctaaag gcaatcatg cactacacc aaatgccaa 
      atcatgttt cctgatgcc caggaccaa aatatcccg 
      agtttgaag cgaagtaat gttaatatt tctggaaat 
      atacatagt gtttatacc gctcgtcaa ctgctaatt 
      ggaagtttg ccactcttg attattttc gacttgcct 
      gaagatatt gcaaatctc aaagttcgt tctgaacaa 
      atcgacgaa atccaaaat acttgtgac gtcacaatt 
      actgtaaga caaaagtca aagcagatg attaaagct 
      tgcatcatc aagggcatc gaacggcaa gcttcaaac 
      atatacaag gcccgaaat ttaatcctg ggtatcgac 
      gatccgccc atccaggcc gatattccc cgcctttac 
      cacatccca agcactccg tcgcccaat aatgacctg 
      ttgaccact cccactaac ctaaacccc tcccctttg 
      accccgata ttcacttgg catttttcc tctgaccaa 
      accacgccc tttgaaatt gtccaaaag ccacagatg 
      ttgtacaac aacaacaac aacaagatg caacaatcg 
      tccaacagt agcggtttc caatcgttt gaacaagtc 
      aaagctgat actagcctc ctgtctagt atttccagc 
      aatagtagt aactacgtg tcaagccct ccaacggtg 
      ttttcgccc caaagtgat aataacatc cccactact 
      tctgattac caatcaatg aatgagaaa aagtccgcc 
      agtttcgat tatgactgc agcaagcgt ttggctggt 
      ttcaaggcg atgcagggg cgtccaaac cctgatgtg 
      tatagggtg cccaccgct atgtgggca gggtacggc 
      ataagtcac acaagtcct gctggaatg ttgaaaaaa 
      gaagacgat atctggaaa cccgcagaa gtgtttcca 
      tcaacatca gggcttcac aacactagt aacattcta 
      gaccacact cccacccac tttataaac cgcattgcc 
      aacaccact tggtctgat ttgccgtca cttctaagt 
      gccttaggt ttggaccgt tatatcagt cttttcatt 
      cgacatgaa gttgatttg ccaacgttt acgactctt 
      tccgacaaa gatttgatg acaatcgga gtgacagca 
      ttcggaagt cgtcgcaaa atgctattg gctatctcg 
      gaactcaac aagagaagt tgtacgttt tcggcggct 
      cctggagca gagcgcaaa agttcctct tcgaattcg 
      cccccaagt catgacaac agtcctcgt caaagttgg 
      taa
    mRNA sequence
    • >TC005315 RA
      agtcgcgca aattaccaa aagcgtgtc acctacaca 
      gcccttaaa aacgcgtaa aaatcctta caaagtgac 
      cattaaatg gggttagtc attaattaa ttgtggacg 
      tagtgattt aaaaaaaaa tgtcttgta attgtgcta 
      cacctcgtg gaatcgagg taaaaaatt ccgacacac 
      aatcagaag tgtcggaaa gcggcacgg ctacttctg 
      gtttcaact caaattggg gctccagag aggaattaa 
      gggattttg tcgccaaac acggattaa aggacattt 
      cggatttgt accaggacc ggttccggg tcgaccgca 
      aaaagttgg aacagatgc ttagtgggg acaacgatg 
      cactgatcc ctgctgacg ttttctttg cgaacatta 
      tggaagaca ctgacactt acataacgt ggccaaaca 
      aactcaaat tgggggcta aatcgaaaa aggatcctc 
      acgtgagga tcgccggca aagaagagg atgttaaag 
      ccgccaaag acaggatta tgacggttt tgtacacga 
      gatgcaatc gtgtaacca tgaaaatgg acataagtt 
      acacggacc attcgcata ttatcggca gaggagggc 
      tgagtatta aacgtgtca tggaagaaa ctcaatgcc 
      acgtacatt tccctgatt cgaatcgtt cgaatccga 
      tggaaaaaa gcaatcaag tttcgattt cgggcgatt 
      tggaagggg ttgaaaatg caaggagtc gcgttaggg 
      aattaattc cgctgattt ttgggtttg agcctccaa 
      taatggccc aaaatattg ataatacgt gtccttacg 
      taattaaaa tccaggaac agtacaaag tgcaagtca 
      tgttcaaaa ctaggtcca aacttcatg ctactatag 
      tactagtca agggagtcg aatgggagg tggagcagg 
      tgaagcagg ctacaattt tgctcatgg aatacatgt 
      gcgaaaatt tgacgaacc aaattccgg ttcaaatga 
      ctatccaaa tttcgcccc aacaccatg caatcgttt 
      tgggcaaaa atcattcaa acctaaagg caatcatgc 
      actacacca aatgccaaa tcatgtttc ctgatgccc 
      aggaccaaa atatcccga gtttgaagc gaagtaatg 
      ttaatattt ctggaaata tacatagtg tttataccg 
      ctcgtcaac tgctaattg gaagtttgc cactcttga 
      ttattttcg acttgcctg aagatattg caaatctca 
      aagttcgtt ctgaacaaa tcgacgaaa tccaaaata 
      cttgtgacg tcacaatta ctgtaagac aaaagtcaa 
      agcagatga ttaaagctt gcatcatca agggcatcg 
      aacggcaag cttcaaaca tatacaagg cccgaaatt 
      taatcctgg gtatcgacg atccgccca tccaggccg 
      atattcccc gcctttacc acatcccaa gcactccgt 
      cgcccaata atgacctgt tgaccactc ccactaacc 
      taaacccct cccctttga ccccgatat tcacttggc 
      atttttcct ctgaccaaa ccacgccct ttgaaattg 
      tccaaaagc cacagatgt tgtacaaca acaacaaca 
      acaagatgc aacaatcgt ccaacagta gcggtttcc 
      aatcgtttg aacaagtca aagctgata ctagcctcc 
      tgtctagta tttccagca atagtagta actacgtgt 
      caagccctc caacggtgt tttcgcccc aaagtgata 
      ataacatcc ccactactt ctgattacc aatcaatga 
      atgagaaaa agtccgcca gtttcgatt atgactgca 
      gcaagcgtt tggctggtt tcaaggcga tgcaggggc 
      gtccaaacc ctgatgtgt atagggtgc ccaccgcta 
      tgtgggcag ggtacggca taagtcaca caagtcctg 
      ctggaatgt tgaaaaaag aagacgata tctggaaac 
      ccgcagaag tgtttccat caacatcag ggcttcaca 
      acactagta acattctag accacactc ccacccact 
      ttataaacc gcattgcca acaccactt ggtctgatt 
      tgccgtcac ttctaagtg ccttaggtt tggaccgtt 
      atatcagtc ttttcattc gacatgaag ttgatttgc 
      caacgttta cgactcttt ccgacaaag atttgatga 
      caatcggag tgacagcat tcggaagtc gtcgcaaaa 
      tgctattgg ctatctcgg aactcaaca agagaagtt 
      gtacgtttt cggcggctc ctggagcag agcgcaaaa 
      gttcctctt cgaattcgc ccccaagtc atgacaaca 
      gtcctcgtc aaagttggt aattagaca gttgtacca 
      atcattgtg acttttgtt tgctatttg tattttgtt 
      ttaattgtt gtgttgttg gagattttg gaaaattgg 
      ttgttggtg attgataaa attccgcaa attatttct 
      taaaaattc tgcgcgcat ttttatagc aataatctg 
      gagggagtt ttatgtgta ctcgtttta taattaagc 
      cgatcaatt gtgatattt tatgagcat tgccccatt 
      ttcatatca tgttcatat agtttttaa ctggagtaa 
      ttttaagga cagattttg taagcaaaa gttttaatt 
      tgcttcttt tgattattt gattcactt tttgtacct 
      tttatgaga taaattaaa acttttgta ccctgtgat 
      gtaaatcct tattctact acatataat ttattgtta 
      ttattataa aaactacca ttattatta ttattatta 
      ttgatgctt aaagcaatt attacaaat gaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY