Status
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Gene information
General information
TC006788 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14289300..14300592
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006788
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06391

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     antenna complete not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen segment slightlynumber decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment potentially partiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006788 RA
      MAPYFTGYP FQGQGRVNQ LGGVFINGR PLPNHIRLK 
IVEMAAAGI RPCVISRQL RVSHGCVSK ILNRYQETG 
SIRPGVIGG SKPRVATLE VEARIEQLK KEEPQIFSW 
EIRDRLIKE GICDKNSAP SVSSISRLL RGGRKEDPD 
RKNHSIEGI LGPNSSCDE SDTESEPGI PLKRKQRRS 
RTTFTGEQL EALERAFGR TQYPDVYTR EELAQKTKL 
TEARVQVWF SNRRARLRK QLNSQQLNA FNSMSLPSA 
FPVQHQYGD PAFNQSTWA QQSYASAAL SSGALPHSA 
LTTGGASTS VASSTPNTL YNSYNSAAT LDQNNQFGY 
NMAQVSPNQ HSVSPAAHQ QSAVWSRQN NKVAENLSS 
WHENYSLFA GSHYGAHSP SEAKSGYPY LGTMDMCAS 
RVH
    CDS sequence
    • >TC006788 RA
      atggcaccg tatttcact ggataccca ttccaaggt 
cagggccgt gtaaaccaa ctcggaggt gtgtttatc 
aacggacgt cctttaccg aatcacatc agactgaaa 
atcgtcgaa atggcagca gcgggcata agaccctgc 
gtgatttca agacagtta agggtttca cacggttgt 
gtttcgaaa attttgaac cgttatcag gaaactgga 
agcataaga ccgggagtt atcggcggt tcgaagcca 
agagttgca acgttggaa gttgaggca aggatagaa 
caactcaaa aaggaggaa ccgcaaatt ttctcgtgg 
gaaattaga gacagatta atcaaggag ggcatttgc 
gacaagaat tcggccccg tctgtgagc tccattagc 
cgtttattg cgcggagga agaaaagaa gatccggat 
cgaaaaaac cactcgatc gaaggtatt cttggaccg 
aattcttcc tgtgacgaa agcgacacc gaatcagaa 
ccgggaatc ccgctaaaa agaaaacag cgtcgttcc 
cgaacgact ttcaccggc gaacaactc gaagcttta 
gaacgagct ttcggtcgt acccaatac cccgatgtt 
tacaccagg gaagaactg gcccaaaaa acgaaacta 
acggaagca cgagttcaa gtttggttt tcgaatcga 
agggcacgt ctccgaaaa caactaaat tcgcaacaa 
ctcaacgct tttaattcc atgtcacta ccgtcggct 
tttcccgtg cagcaccag tacggcgat cctgccttt 
aatcaaagc acttgggcg caacagagc tacgcttcg 
gcagctctg agcagtgga gctcttcct catagtgcc 
ctaactact ggtggtgca agcacaagt gtggcatcg 
tcaacaccc aatacttta tacaacagt tacaattcg 
gctgcgact ttagatcaa aacaaccaa ttcggttac 
aatatggcc caggtttcg ccgaatcag catagcgtt 
tcgccagcg gctcatcag cagagtgcc gtttggtcg 
agacaaaac aataaagtc gctgaaaat ctgagcagc 
tggcacgag aattacagc cttttcgct ggtagccat 
tatggggcc cattcaccc agcgaggcc aaatcggga 
tacccatat ctgggcacc atggacatg tgcgcaagt 
cgagtgcac taa
    mRNA sequence
    • >TC006788 RA
      ggtcagttt tcttcaaga agttcaact ggtacgacg 
aaacgcgtt cggcttagt ttttctaaa cggtttagg 
tcgtgttgg gtggatgtt cagtgattt ctaaacatt 
ttttttcac gtttttggg aataaatgg cagtttcta 
cactcaata tggcaccgt atttcactg gatacccat 
tccaaggtc agggccgtg taaaccaac tcggaggtg 
tgtttatca acggacgtc ctttaccga atcacatca 
gactgaaaa tcgtcgaaa tggcagcag cgggcataa 
gaccctgcg tgatttcaa gacagttaa gggtttcac 
acggttgtg tttcgaaaa ttttgaacc gttatcagg 
aaactggaa gcataagac cgggagtta tcggcggtt 
cgaagccaa gagttgcaa cgttggaag ttgaggcaa 
ggatagaac aactcaaaa aggaggaac cgcaaattt 
tctcgtggg aaattagag acagattaa tcaaggagg 
gcatttgcg acaagaatt cggccccgt ctgtgagct 
ccattagcc gtttattgc gcggaggaa gaaaagaag 
atccggatc gaaaaaacc actcgatcg aaggtattc 
ttggaccga attcttcct gtgacgaaa gcgacaccg 
aatcagaac cgggaatcc cgctaaaaa gaaaacagc 
gtcgttccc gaacgactt tcaccggcg aacaactcg 
aagctttag aacgagctt tcggtcgta cccaatacc 
ccgatgttt acaccaggg aagaactgg cccaaaaaa 
cgaaactaa cggaagcac gagttcaag tttggtttt 
cgaatcgaa gggcacgtc tccgaaaac aactaaatt 
cgcaacaac tcaacgctt ttaattcca tgtcactac 
cgtcggctt ttcccgtgc agcaccagt acggcgatc 
ctgccttta atcaaagca cttgggcgc aacagagct 
acgcttcgg cagctctga gcagtggag ctcttcctc 
atagtgccc taactactg gtggtgcaa gcacaagtg 
tggcatcgt caacaccca atactttat acaacagtt 
acaattcgg ctgcgactt tagatcaaa acaaccaat 
tcggttaca atatggccc aggtttcgc cgaatcagc 
atagcgttt cgccagcgg ctcatcagc agagtgccg 
tttggtcga gacaaaaca ataaagtcg ctgaaaatc 
tgagcagct ggcacgaga attacagcc ttttcgctg 
gtagccatt atggggccc attcaccca gcgaggcca 
aatcgggat acccatatc tgggcacca tggacatgt 
gcgcaagtc gagtgcact aaaattttt cccatttcg 
tcagcttac accacactg cgctggact tactactaa 
cataagggc tttattatt ttagttgtt ttttttgta 
cttattttc agtgccacc attgtacta ctattgtaa 
tcactgtac atacaagtg tattttaag cgtagtttt 
agcgaatga gtagctgtt aattataca ataactact 
gtttctgtg aattttatt tagctgtat tcacttaga 
gattaataa atttcttgc aataca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY