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Gene information
General information
TC006771 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14000410..14009180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006771
    iBeetle screen iB_06390

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: In strong cases only the head is remaining.
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: In strong cases only the head is remaining.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006771 RA
      MLQQILRDM YVDPDILAE LDEQQKQTL FCKMREEQV 
RRWKLWNEK LGDEKTIQP SSKKKTVSF LKGEDGEPW 
VWVMGEHKD DKTIEEILK EEAIEKARK MAEKEAEEL 
RKQVEAQLY IELTPKIEE MEVAPKIPI DDDIYCSVD 
ELREKINNK PDHFNYSLN SYQSKNKFN VIDTRDVLQ 
EISLNTQKV AQRVALWEK RLTEERTCE IFQKMQKEQ 
QQVAKEAEE AEQKQEQLW REQERKAKQ AEQQIREIA 
RRAREEHRL TNFEIDTSY STINNTGVP PGRGAVVEW 
YKSHERPKW TGLDRSNNV ESWFHGLIT RSEAEKLLL 
DRPYGSFLV RLSERIWGY AISYRAKEK CKHYLVSAG 
LKYQFLGNN QIEHETLRD LIEYHRVHP LTGGEKLVE 
ACPRSATSP LNELFDE
    CDS sequence
    • >TC006771 RA
      atgttgcag caaatcctt cgggacatg tacgtggac 
ccggacatc ctcgccgaa ctggacgag caacaaaaa 
cagaccctc ttctgcaaa atgcgtgaa gagcaagtc 
cggagatgg aagctctgg aacgaaaaa ctgggcgat 
gaaaaaaca atccaacct tccagtaag aaaaaaact 
gtgagtttc ctcaaaggc gaagatggg gagccatgg 
gtgtgggtg atgggagaa cacaaagac gacaaaacc 
atcgaggag atcctgaag gaggaagcc attgaaaaa 
gcccggaaa atggccgag aaagaagcc gaagagttg 
cgaaaacaa gtcgaagcc caactttac atcgaactc 
accccgaaa atcgaggaa atggaagtc gcccccaaa 
atcccaatc gacgatgat atctactgt tcggttgat 
gagttaagg gagaagatt aacaacaaa ccggaccat 
tttaactat agtttgaat tcgtaccag agtaagaat 
aagttcaat gtgattgac acgcgggac gttttgcaa 
gagatctcg ctcaacacg cagaaagtg gcacagagg 
gttgcccta tgggagaag agactgact gaggagcga 
acgtgtgag atcttccag aagatgcaa aaggagcag 
cagcaggtg gccaaggag gcggaggag gcggaacaa 
aagcaggag caactttgg agggaacaa gaacgcaaa 
gccaaacaa gccgaacag caaatccgc gaaatcgcg 
cgccgcgct cgagaggaa cacagactg acaaacttc 
gaaatcgac acctcctac agcacgatc aacaacacg 
ggggttccc ccgggtcgt ggcgcagtc gtcgaatgg 
tacaaaagc cacgagagg cccaaatgg actggcttg 
gaccggtcc aacaacgtg gagtcgtgg ttccatggc 
ttgatcacg cgatctgag gccgaaaag ctgctgctg 
gacaggccg tacgggagc tttctggtc aggctgtcc 
gagcgaatt tggggatat gccatttcg tacagggcc 
aaggagaag tgcaaacac tacctggtc agtgcagga 
ctcaagtac cagtttttg ggcaacaac caaatcgag 
cacgaaacg ctgcgtgat ctcatcgaa taccaccgg 
gttcacccc ctcaccggg ggcgagaag ctggtggag 
gcctgcccc aggagcgcc acctccccc cttaatgaa 
ctgttcgac gaatga
    mRNA sequence
    • >TC006771 RA
      cgatttaac aaaccttga atgagcaaa gacagacat 
atgaggtga aagcctcat caaacagcg catttttcc 
tgcacttgt gccccattt gttcgacac ttcttcgat 
cgcagcaaa ttgttgcca cctcctgct gttgagccc 
acaattcaa cttgattca gtcggcttt cggtcgttg 
tagagtagg tttgtgaga ctcctgcta gtgcttgcc 
gtctttacc acacacatg ggcgctatt tgagtcgct 
ttagtgagc tgtgcagtg ccaaagtgc ctttctatg 
gaatattag acgcagttg cgacaaaaa gaacccata 
atgttgcag caaatcctt cgggacatg tacgtggac 
ccggacatc ctcgccgaa ctggacgag caacaaaaa 
cagaccctc ttctgcaaa atgcgtgaa gagcaagtc 
cggagatgg aagctctgg aacgaaaaa ctgggcgat 
gaaaaaaca atccaacct tccagtaag aaaaaaact 
gtgagtttc ctcaaaggc gaagatggg gagccatgg 
gtgtgggtg atgggagaa cacaaagac gacaaaacc 
atcgaggag atcctgaag gaggaagcc attgaaaaa 
gcccggaaa atggccgag aaagaagcc gaagagttg 
cgaaaacaa gtcgaagcc caactttac atcgaactc 
accccgaaa atcgaggaa atggaagtc gcccccaaa 
atcccaatc gacgatgat atctactgt tcggttgat 
gagttaagg gagaagatt aacaacaaa ccggaccat 
tttaactat agtttgaat tcgtaccag agtaagaat 
aagttcaat gtgattgac acgcgggac gttttgcaa 
gagatctcg ctcaacacg cagaaagtg gcacagagg 
gttgcccta tgggagaag agactgact gaggagcga 
acgtgtgag atcttccag aagatgcaa aaggagcag 
cagcaggtg gccaaggag gcggaggag gcggaacaa 
aagcaggag caactttgg agggaacaa gaacgcaaa 
gccaaacaa gccgaacag caaatccgc gaaatcgcg 
cgccgcgct cgagaggaa cacagactg acaaacttc 
gaaatcgac acctcctac agcacgatc aacaacacg 
ggggttccc ccgggtcgt ggcgcagtc gtcgaatgg 
tacaaaagc cacgagagg cccaaatgg actggcttg 
gaccggtcc aacaacgtg gagtcgtgg ttccatggc 
ttgatcacg cgatctgag gccgaaaag ctgctgctg 
gacaggccg tacgggagc tttctggtc aggctgtcc 
gagcgaatt tggggatat gccatttcg tacagggcc 
aaggagaag tgcaaacac tacctggtc agtgcagga 
ctcaagtac cagtttttg ggcaacaac caaatcgag 
cacgaaacg ctgcgtgat ctcatcgaa taccaccgg 
gttcacccc ctcaccggg ggcgagaag ctggtggag 
gcctgcccc aggagcgcc acctccccc cttaatgaa 
ctgttcgac gaatgaatt gttcagtct caaagaaaa 
ctctgtgat cgtttggca tttttatta ctactggaa 
cgattctag ttcagtatt aatcgcact tatcgcttt 
tataatcga gtttgtttc aatttgtgc cttgtctag 
ttattttat tatttatgt gaacataat cggttgtat 
gccccattt tagggcaga ctacatgaa ataaacgat 
tttgcttaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY