Status
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Gene information
General information
TC032086
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000858
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7140488..7141963
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032086
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06389

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032086 RA
      MLTPSSSSN RDVSTISTE TWSSWYFTD NGIGTSIDN 
      DTSTEYSNP ENFEKQICD LQALRQKLL EEEKKKETF 
      LKQVNAEGK FDQARLEAE KNILLATLK TSLLLAEFK 
      HKYESQRER DYFNIGSVT ISDIKLHCD KTQYLKKFK 
      HYFLCALTT GSKLFLSQI VQADSKDVI NFNINHTFL 
      NLPVDFEIG VRIYILRHR KKTITDKLL GWFKRGVPE 
      ENSYRRNSL FKLWKEASI KISNVNETN EIILDLFDS 
      KLCKLVYDV TAKVDTSSF LYSGWLDVS QNGLFWNQR 
      WCVLQGTLL KCFNYPVDH SFNNPLTTI DLKYCVTIR 
      DPFMYEASR NRVLVLEMN DDSHPFAYY LSTYSVHEF 
      NQWKCLEKL VATLQLWAK YNCKII
    CDS sequence
    • >TC032086 RA
      atgttaaca ccatcgagt tccagtaat agagacgtt 
      tcaacaatt tcaacagag acttggtca tcatggtat 
      ttcaccgat aacgggatt ggaacgtcg atagacaat 
      gacacaagc actgaatat tcaaacccc gaaaatttt 
      gaaaagcaa atttgtgat ttgcaagca ctacgacaa 
      aaattgctt gaggaagaa aagaaaaaa gagacgttt 
      ttgaagcaa gttaatgct gaaggcaag ttcgatcaa 
      gcaagatta gaagctgag aaaaatatt ttgttagca 
      acacttaaa accagtctt ctattggcc gagttcaaa 
      cataaatat gaatcgcaa agagagagg gattatttt 
      aacatcggt tcggttacc atttccgac atcaagttg 
      cactgcgac aaaacccaa tatttgaaa aaattcaaa 
      cactatttt ctatgtgcg ctgactact ggcagtaaa 
      ttattttta agtcagatc gtacaagca gactccaag 
      gatgttata aacttcaac ataaatcat acgtttctt 
      aatttaccg gtcgatttc gagatcgga gtcagaatt 
      tatatttta cggcatcgg aagaagaca attacagat 
      aagttgttg ggttggttt aaacgtggt gttccagaa 
      gagaatagc taccgacgt aactccttg ttcaaatta 
      tggaaagag gcgtcgatt aaaatttcg aatgttaac 
      gaaacgaac gagataatc ttggacttg tttgactca 
      aaattgtgt aaattagtg tatgacgta acggcgaaa 
      gtcgacact tcgagtttc ttgtattcg ggatggctg 
      gatgtgagt cagaatgga ttgttttgg aatcagagg 
      tggtgtgtt ttgcaggga actctattg aaatgtttc 
      aactaccct gtagatcat agtttcaat aatccgttg 
      actacaatt gatttaaaa tattgtgta accattaga 
      gatcctttt atgtatgag gcttccaga aatagggtt 
      ttggttcta gaaatgaac gatgattct catcctttt 
      gcttattat ttatcaaca tattcagtg catgaattt 
      aatcaatgg aagtgtctt gaaaaattg gtggcgaca 
      ttgcaactt tgggcaaaa tacaattgt aagattata 
      tga
    mRNA sequence
    • >TC032086 RA
      ggtcgggtg ccaacattt agggtgtca tttggcttt 
      caagctcag tttatattt gtgctttgt cagtgattg 
      gcctcgaaa caaaaatgt taacaccat cgagttcca 
      gtaatagag acgtttcaa caatttcaa cagagactt 
      ggtcatcat ggtatttca ccgataacg ggattggaa 
      cgtcgatag acaatgaca caagcactg aatattcaa 
      accccgaaa attttgaaa agcaaattt gtgatttgc 
      aagcactac gacaaaaat tgcttgagg aagaaaaga 
      aaaaagaga cgtttttga agcaagtta atgctgaag 
      gcaagttcg atcaagcaa gattagaag ctgagaaaa 
      atattttgt tagcaacac ttaaaacca gtcttctat 
      tggccgagt tcaaacata aatatgaat cgcaaagag 
      agagggatt attttaaca tcggttcgg ttaccattt 
      ccgacatca agttgcact gcgacaaaa cccaatatt 
      tgaaaaaat tcaaacact attttctat gtgcgctga 
      ctactggca gtaaattat ttttaagtc agatcgtac 
      aagcagact ccaaggatg ttataaact tcaacataa 
      atcatacgt ttcttaatt taccggtcg atttcgaga 
      tcggagtca gaatttata ttttacggc atcggaaga 
      agacaatta cagataagt tgttgggtt ggtttaaac 
      gtggtgttc cagaagaga atagctacc gacgtaact 
      ccttgttca aattatgga aagaggcgt cgattaaaa 
      tttcgaatg ttaacgaaa cgaacgaga taatcttgg 
      acttgtttg actcaaaat tgtgtaaat tagtgtatg 
      acgtaacgg cgaaagtcg acacttcga gtttcttgt 
      attcgggat ggctggatg tgagtcaga atggattgt 
      tttggaatc agaggtggt gtgttttgc agggaactc 
      tattgaaat gtttcaact accctgtag atcatagtt 
      tcaataatc cgttgacta caattgatt taaaatatt 
      gtgtaacca ttagagatc cttttatgt atgaggctt 
      ccagaaata gggttttgg ttctagaaa tgaacgatg 
      attctcatc cttttgctt attatttat caacatatt 
      cagtgcatg aatttaatc aatggaagt gtcttgaaa 
      aattggtgg cgacattgc aactttggg caaaataca 
      attgtaaga ttatatgat taagtaaat caaatattt 
      gtgagatac actgatgta ataattata attataaaa 
      taaaatatt ggttgaaga ataataaaa aactcaagc 
      agat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY