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Gene information
General information
TC006753 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13731729..13765414
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006753
    iBeetle screen iB_06386

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006753 RA
      MTSRPGSGS KTSTGHRRA ESGGGHHRH SSSGSGHHR 
      SDSGQSFDS DYPRERKHG HHRSKSGVN ERERLDSNG 
      SQLSNKLEF RSAQGLHDG HYPRPTSLF PPKSDALGP 
      DALQPEREP IRFDVNLVG APPEVEQLV NNIKQVAEQ 
      FLYHWKTFP IILPSPVSQ CDSFPTSNV SSDGSLGRC 
      KTRYHLRDL FVAPSFDEL DAVAVDGKG EPRRLTGKQ 
      LEYIRERGE FEVESLNFP GQQHRWKLS QLLQKGTEK 
      SRDSLLNDL ALALRFIFI TAKGRLVSH FFSVSEAIK 
      ALLAGLLRI IDLVFGIPS LQAHNLDAK IREERTKYL 
      VAELVCRPE NEESLEHLC SYIRKQLRR AATEKFEVT 
      KECSQPPVA VPYRFLTPS GSELDLRLW ERSLMRRAL 
      PTLVAILER ETRGWFLHF RERLIAELR AQKMPDEDI 
      EREVNDAVM KEYLQRVYN SILSHPDIL SLGEGIAQL 
      LVQQAQSVV LMHRAVENV QHRLSKSQE EIKTRLCTS 
      HPVLSRIEP WLRSRLRSA ELKFSQENQ WSAHEEALT 
      LCNAKRLHQ TVYFLNRDL AFMREREPA LLRELRKVK 
      TPTRSFLWP TQIWLPTNW IVRRNFQGQ SEVVPTVLS 
      KQATSITTP RSDPSQPVF LVEKEIVRT TTTRWPLWR 
      IFNYFHRTW CWTWNAMFF FGIVLPWCS PVGLRALFS 
      LEPFMPDLE LSQVNGTLF PRKSSLTET LCSRLISLW 
      RHISKSRTH FETKPDTGF IGKGFTRHL NRIWNYFFK 
      GLFGTIVLV VIFPVVCVA VIAASVFIA LTAVVWMPV 
      FTLAVQIIN ALIYDLDCP EQKRNRFFV LLEALVWKI 
      GLQGCVQPI VALFVAAVV CPIIALVIL TGGIARYWL 
      RLFWDTTLF HLIIKKRGR IPASDSFVV KRIAGPGLA 
      SDYYFQISP EQALAAFEA KMEWDELSA YQTVVENTI 
      TQPQRDFSH FIEACFGGF SAQLAKNGP YKNLEKEAQ 
      DLMTVLHEK LERRRRDLQ TGLSVTVKS KIKLETSEL 
      KIAIQQGAL MLERFYPTH ILPKLGCSE DQFWDNRAL 
      SPGDWAGLA GLLYSELFS LDILTPLDH TDTSFRLDP 
      HPQADLSRY TEMVQCAQL GARGPDLLG NVYTPRGNI 
      QVHSPYLEV SAFNPRSKL ATTVKKCEK KTESLTMLG 
      SIKSGTVWT PWKKHSRPF SPDKMVIPL PVPHPAHIA 
      VTIFNRDSD DPIPLESDI CQSILRGIE ESHISLEES 
      SGGINRYKG GGDSFETSS SSETLDSPH NGLQSNRNS 
      SDGVYQWTL NNWGQRKRN NSGSVKVDL ASPEDVTLD 
      SDSTRVVFS TYGTTV
    CDS sequence
    • >TC006753 RA
      atgacgtct cggcccggc tcgggctcg aaaacgtca 
      acggggcac cggagggcc gaatccggg ggcggccac 
      caccggcac agttcgtcc gggtccggc caccaccgc 
      tccgattcg ggccaaagc ttcgattcc gattatccc 
      cgagaacgc aaacacggc catcatcgc tcaaagtcg 
      ggtgttaac gaacgcgaa cgactagac tcgaacgga 
      tcgcaactg tcgaacaaa ctcgaattt cgctcagct 
      caaggcttg cacgatggc cactacccg cgccccacg 
      tcacttttc ccacccaaa tccgacgct ttaggccct 
      gacgccctc caacccgaa cgggagccg atcagattc 
      gacgtgaat ttggtgggg gctcccccg gaagtggag 
      cagttggtt aataatatt aagcaagtc gccgaacag 
      tttctatac cattggaag actttcccg ataatcctt 
      ccaagtcca gtctcacaa tgtgattca tttccgact 
      tcaaatgtt tcgtctgat gggagtttg gggaggtgt 
      aagacgagg tatcaccta cgtgatttg ttcgtagcg 
      ccatctttt gatgaactt gatgctgtg gcggtcgat 
      ggcaaaggt gagccgaga aggttgacc ggaaaacaa 
      ttggagtat ataagggag agaggcgag tttgaagta 
      gagtctttg aattttccc ggccaacaa caccgctgg 
      aaactgtcc cagctcctg cagaaaggc accgagaaa 
      agccgcgat tccctcctc aatgatctc gcgctagcg 
      ctgcgattt atttttata acggcgaaa ggacgcctc 
      gtctcgcat ttcttcagc gtttcggaa gcgatcaag 
      gctctcttg gccggactt ttgcgcatt atcgacctc 
      gtcttcggc attccaagt ttgcaagct cacaattta 
      gatgccaaa attagggag gagaggacc aagtatcta 
      gtcgccgaa ctggtgtgc cggcccgaa aatgaggaa 
      tctctggaa cacctgtgt tcctacatc cgtaagcag 
      ctgcgtcgg gccgccacc gaaaaattc gaagtgaca 
      aaggagtgt tcgcagccg cctgtcgct gttccctac 
      cgatttttg actccctcg ggatctgag ctggacttg 
      aggctctgg gagaggagt ctgatgagg cgtgccttg 
      cccacgttg gttgctatt ttggaaagg gagacgcga 
      ggctggttt ttacatttt agagaaaga ctgattgct 
      gagttgcga gctcaaaaa atgcccgat gaagatata 
      gaaagggaa gtgaacgat gctgttatg aaggaatat 
      ttgcagagg gtttataac agcattttg tcgcatccc 
      gatatttta agtttaggc gaagggatt gctcaactt 
      ttagtacaa caggcccaa agcgttgtt ttaatgcac 
      agggccgtc gagaacgtt caacaccgt ctttcaaaa 
      tctcaggaa gaaatcaaa acccgcctc tgcaccagc 
      caccctgtc ctctcccgc atcgagcct tggctgcgt 
      tcgcgcctc cgttccgcc gaactgaaa ttcagtcaa 
      gagaaccaa tggagcgcc cacgaggaa gccctcact 
      ttatgcaac gcgaaacgc ctccaccag acggtttac 
      ttcctcaac cgtgacctc gcattcatg agagaaaga 
      gaacctgcc cttttacgc gaactgcgc aaagtcaaa 
      accccgaca cgttcgttc ctctggccg acccaaatc 
      tggctcccg accaattgg attgtgcga agaaacttc 
      caaggccaa tcagaagtc gtcccgact gtcctcagc 
      aaacaagcg actagcatc acgacgcct aggtccgac 
      cccagccaa cccgtcttc ctggtggag aaggagatc 
      gtccggacg accaccacc aggtggccg ctctggcgc 
      attttcaat tacttccac cggacgtgg tgttggact 
      tggaacgcg atgtttttc ttcgggatc gtgttgccg 
      tggtgtagt cctgtggga ctccgggcg cttttcagt 
      ctcgagccc tttatgccc gacctggag ctgtcccaa 
      gtcaatggg acgctcttc ccgcgtaaa agcagtctc 
      acggagacg ttgtgctcg cgtctgatt agcctctgg 
      aggcacatt tccaagtcc aggacgcac tttgagact 
      aaacctgat acggggttc attgggaaa ggcttcacg 
      cggcatttg aaccgaatt tggaactat tttttcaag 
      ggattgttc gggacgatt gtcctagtt gtgattttt 
      cccgtggtg tgtgtcgct gtgatcgca gcgagtgtt 
      tttattgcc ttgacggcg gtggtgtgg atgcctgtt 
      tttacattg gcggtgcaa ataatcaat gcgttgatt 
      tatgatctg gactgtccg gaacagaag aggaaccga 
      tttttcgta ctgttggaa gcactggtt tggaagatt 
      gggcttcaa gggtgcgtg caaccgatt gttgcactt 
      tttgtggcc gcggttgtg tgccccatt attgcgttg 
      gtgattttg acaggtggc attgctcgc tactggctg 
      cgcctcttc tgggacacg accctcttc cacttaata 
      atcaaaaaa cgtggccgt atcccagcc agtgacagt 
      ttcgttgtg aaacgcatc gcaggaccg ggcctagcc 
      tccgactac tacttccaa atcagtcca gagcaggcc 
      cttgccgct ttcgaggcc aaaatggaa tgggacgaa 
      ctgtccgca taccaaacc gtcgtcgaa aacacaatc 
      acacaacca cagcgtgac tttagccac tttatcgaa 
      gcgtgtttc ggtggcttt tcggcacag ttggccaaa 
      aacggccct tataaaaat ctcgaaaag gaagcccaa 
      gacctgatg acggtcctg cacgagaaa ctcgaacgg 
      agacgacgt gacttacaa accggtctc agtgtcacc 
      gttaaaagt aaaattaaa ttggagacg tccgaattg 
      aaaattgct attcaacaa ggggcgctg atgcttgag 
      aggttttat ccaacgcat attttaccg aaattgggg 
      tgcagtgag gaccagttt tgggataat cgggctttg 
      agtcccggg gattgggcg ggattggct ggtttgttg 
      tattcggaa cttttcagt ttggatatt ttaacgccg 
      ttggatcat acggatacg agttttcgg ctggatccg 
      catccgcaa gctgatttg tctaggtat acggagatg 
      gtgcagtgt gcacagttg ggggctcgg gggcccgat 
      ctgttgggg aatgtttac acgccaagg gggaatatt 
      caagtgcat tccccctat ttggaagtg tcggcgttt 
      aatcccaga tctaagctc gccacgacg gtgaaaaag 
      tgcgagaag aagacggag tcgttgacg atgttgggt 
      tcgatcaag agtggcaca gtttggacg ccttggaag 
      aagcactca cggccgttt tcgcccgat aaaatggtc 
      attcctttg cccgttccg caccccgca cacattgcc 
      gttacgatt tttaaccgc gattcagac gatcccatt 
      cccttggaa tccgacatt tgtcagagt attttacgc 
      ggtattgaa gagagtcac atttcgctt gaggagtca 
      tccggtggt ataaatagg tataaaggc gggggcgac 
      tcgtttgag acaagctcg agttcggaa acgctcgac 
      agcccccat aacggtctc caatcgaac cgcaattcc 
      agcgacggt gtctaccag tggacgtta aacaactgg 
      ggtcagcgc aaacgtaat aattcaggt agtgttaag 
      gtcgacttg gccagtccc gaggacgtt actttagac 
      agtgacagt actcgggtg gttttcagt acgtacggt 
      acgaccgtt tga
    mRNA sequence
    • >TC006753 RA
      tgtcgaaca aatgccgat cgccgaggc atcgcgact 
      cccaagaag ggtgtagaa aatagaaag aaacaggtt 
      tattttaag taaattaaa gagaagttg aaaggaatg 
      cggtgtgtt agtgcccca aattcgcgt cgaagaccg 
      tgaggcatg tcaagtgtg gcgtgtgat tgtgtgtta 
      ccgaaatta tgtgtcgtt tactcgttt gaaataatt 
      gctcttgtt tgttgcaag tgtcatcaa caaaacacc 
      gattacagc actcgattc tgctaaaaa aatgattga 
      gcgccacaa gccgggccc cacatagcc tccaggatg 
      acgtctcgg cccggctcg ggctcgaaa acgtcaacg 
      gggcaccgg agggccgaa tccgggggc ggccaccac 
      cggcacagt tcgtccggg tccggccac caccgctcc 
      gattcgggc caaagcttc gattccgat tatccccga 
      gaacgcaaa cacggccat catcgctca aagtcgggt 
      gttaacgaa cgcgaacga ctagactcg aacggatcg 
      caactgtcg aacaaactc gaatttcgc tcagctcaa 
      ggcttgcac gatggccac tacccgcgc cccacgtca 
      cttttccca cccaaatcc gacgcttta ggccctgac 
      gccctccaa cccgaacgg gagccgatc agattcgac 
      gtgaatttg gtgggggct cccccggaa gtggagcag 
      ttggttaat aatattaag caagtcgcc gaacagttt 
      ctataccat tggaagact ttcccgata atccttcca 
      agtccagtc tcacaatgt gattcattt ccgacttca 
      aatgtttcg tctgatggg agtttgggg aggtgtaag 
      acgaggtat cacctacgt gatttgttc gtagcgcca 
      tcttttgat gaacttgat gctgtggcg gtcgatggc 
      aaaggtgag ccgagaagg ttgaccgga aaacaattg 
      gagtatata agggagaga ggcgagttt gaagtagag 
      tctttgaat tttcccggc caacaacac cgctggaaa 
      ctgtcccag ctcctgcag aaaggcacc gagaaaagc 
      cgcgattcc ctcctcaat gatctcgcg ctagcgctg 
      cgatttatt tttataacg gcgaaagga cgcctcgtc 
      tcgcatttc ttcagcgtt tcggaagcg atcaaggct 
      ctcttggcc ggacttttg cgcattatc gacctcgtc 
      ttcggcatt ccaagtttg caagctcac aatttagat 
      gccaaaatt agggaggag aggaccaag tatctagtc 
      gccgaactg gtgtgccgg cccgaaaat gaggaatct 
      ctggaacac ctgtgttcc tacatccgt aagcagctg 
      cgtcgggcc gccaccgaa aaattcgaa gtgacaaag 
      gagtgttcg cagccgcct gtcgctgtt ccctaccga 
      tttttgact ccctcggga tctgagctg gacttgagg 
      ctctgggag aggagtctg atgaggcgt gccttgccc 
      acgttggtt gctattttg gaaagggag acgcgaggc 
      tggttttta cattttaga gaaagactg attgctgag 
      ttgcgagct caaaaaatg cccgatgaa gatatagaa 
      agggaagtg aacgatgct gttatgaag gaatatttg 
      cagagggtt tataacagc attttgtcg catcccgat 
      attttaagt ttaggcgaa gggattgct caactttta 
      gtacaacag gcccaaagc gttgtttta atgcacagg 
      gccgtcgag aacgttcaa caccgtctt tcaaaatct 
      caggaagaa atcaaaacc cgcctctgc accagccac 
      cctgtcctc tcccgcatc gagccttgg ctgcgttcg 
      cgcctccgt tccgccgaa ctgaaattc agtcaagag 
      aaccaatgg agcgcccac gaggaagcc ctcacttta 
      tgcaacgcg aaacgcctc caccagacg gtttacttc 
      ctcaaccgt gacctcgca ttcatgaga gaaagagaa 
      cctgccctt ttacgcgaa ctgcgcaaa gtcaaaacc 
      ccgacacgt tcgttcctc tggccgacc caaatctgg 
      ctcccgacc aattggatt gtgcgaaga aacttccaa 
      ggccaatca gaagtcgtc ccgactgtc ctcagcaaa 
      caagcgact agcatcacg acgcctagg tccgacccc 
      agccaaccc gtcttcctg gtggagaag gagatcgtc 
      cggacgacc accaccagg tggccgctc tggcgcatt 
      ttcaattac ttccaccgg acgtggtgt tggacttgg 
      aacgcgatg tttttcttc gggatcgtg ttgccgtgg 
      tgtagtcct gtgggactc cgggcgctt ttcagtctc 
      gagcccttt atgcccgac ctggagctg tcccaagtc 
      aatgggacg ctcttcccg cgtaaaagc agtctcacg 
      gagacgttg tgctcgcgt ctgattagc ctctggagg 
      cacatttcc aagtccagg acgcacttt gagactaaa 
      cctgatacg gggttcatt gggaaaggc ttcacgcgg 
      catttgaac cgaatttgg aactatttt ttcaaggga 
      ttgttcggg acgattgtc ctagttgtg atttttccc 
      gtggtgtgt gtcgctgtg atcgcagcg agtgttttt 
      attgccttg acggcggtg gtgtggatg cctgttttt 
      acattggcg gtgcaaata atcaatgcg ttgatttat 
      gatctggac tgtccggaa cagaagagg aaccgattt 
      ttcgtactg ttggaagca ctggtttgg aagattggg 
      cttcaaggg tgcgtgcaa ccgattgtt gcacttttt 
      gtggccgcg gttgtgtgc cccattatt gcgttggtg 
      attttgaca ggtggcatt gctcgctac tggctgcgc 
      ctcttctgg gacacgacc ctcttccac ttaataatc 
      aaaaaacgt ggccgtatc ccagccagt gacagtttc 
      gttgtgaaa cgcatcgca ggaccgggc ctagcctcc 
      gactactac ttccaaatc agtccagag caggccctt 
      gccgctttc gaggccaaa atggaatgg gacgaactg 
      tccgcatac caaaccgtc gtcgaaaac acaatcaca 
      caaccacag cgtgacttt agccacttt atcgaagcg 
      tgtttcggt ggcttttcg gcacagttg gccaaaaac 
      ggcccttat aaaaatctc gaaaaggaa gcccaagac 
      ctgatgacg gtcctgcac gagaaactc gaacggaga 
      cgacgtgac ttacaaacc ggtctcagt gtcaccgtt 
      aaaagtaaa attaaattg gagacgtcc gaattgaaa 
      attgctatt caacaaggg gcgctgatg cttgagagg 
      ttttatcca acgcatatt ttaccgaaa ttggggtgc 
      agtgaggac cagttttgg gataatcgg gctttgagt 
      cccggggat tgggcggga ttggctggt ttgttgtat 
      tcggaactt ttcagtttg gatatttta acgccgttg 
      gatcatacg gatacgagt tttcggctg gatccgcat 
      ccgcaagct gatttgtct aggtatacg gagatggtg 
      cagtgtgca cagttgggg gctcggggg cccgatctg 
      ttggggaat gtttacacg ccaaggggg aatattcaa 
      gtgcattcc ccctatttg gaagtgtcg gcgtttaat 
      cccagatct aagctcgcc acgacggtg aaaaagtgc 
      gagaagaag acggagtcg ttgacgatg ttgggttcg 
      atcaagagt ggcacagtt tggacgcct tggaagaag 
      cactcacgg ccgttttcg cccgataaa atggtcatt 
      cctttgccc gttccgcac cccgcacac attgccgtt 
      acgattttt aaccgcgat tcagacgat cccattccc 
      ttggaatcc gacatttgt cagagtatt ttacgcggt 
      attgaagag agtcacatt tcgcttgag gagtcatcc 
      ggtggtata aataggtat aaaggcggg ggcgactcg 
      tttgagaca agctcgagt tcggaaacg ctcgacagc 
      ccccataac ggtctccaa tcgaaccgc aattccagc 
      gacggtgtc taccagtgg acgttaaac aactggggt 
      cagcgcaaa cgtaataat tcaggtagt gttaaggtc 
      gacttggcc agtcccgag gacgttact ttagacagt 
      gacagtact cgggtggtt ttcagtacg tacggtacg 
      accgtttga gtcatttcc ttgtgattt tccaatttg 
      attggttaa tttttaagt ttccgtgtg atattttag 
      ttacgataa caagcatca ctgtggcat ttttcatat 
      caataataa tcgagtgta gtacaaggg cgtaaaagt 
      ataatggtt tcgttgaga attatgaat aaatgcagg 
      aatttaaat ctaggataa gggcaaata tttagctag 
      taatgcacg tagttatta aatttttta tttgagaaa 
      ttgtccatt gcactagaa tttgtatag tcggatcga 
      tttagaggc aaattaggg gttcgaaac aacagcata 
      gcaataatt tgctgataa ttaaatgtt ttcgttgag 
      ttactacat acttgtcca tatttcaca aatggtagc 
      ttatgacgt tcaaacgaa ttttccact tagcattag 
      agcggaccg ttttaacta ctcgacatt gttttttat 
      tactattgt ttgaccttt tatttatcg tgtacctgt 
      caattacac aaatactct ctgtactcg atattattt 
      tcgtgacgt ttttctatg tgtataaat ttgtatttt 
      tttccatgt ctaattgta caattttta ttgcttgtt 
      gcttgtgtt aattgtatt atatttata aagctgaca 
      ttttgctac gttttgtgt ttactcaga aatataaag 
      ctttaagac tg
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