| General information | |||
| TC006743 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 13623659..13678550 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006743 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06385 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC006743 RA
MIFGRFLAG ALLLTQAGA YHHASQQPP RITEHPVDT TVARHEPAT LNCHASGEP EPTITWFKD GMTLRTAPQ DARSHRVLL PAGNLFFLR VVQSRKESD AGVYWCEAS NALGKARSR NATLTVAVL RDEFRLEPQ STRVAAGED IVMECAPPR GTPEPQVFW RKDGHTLEV EGRLKLVDG SNLAITDTK PSDDGRYQC VAKNTAGVR ESAVAILKV YVKPFLIKP PEDKTALVG STVDFPCAV GGDPLPDVL WRRNAAGGT MPLGRVRVL EDRTLRLER ITLLDQGRY TCEADNYAG AITATALLT VHAPPTLTT RPLAQTVES NRDVSFHCN VEGSPKPFI FWSFEGDRT LIYPGTSTG NYQVTTVEG KSTLTLKQA QPQNSGTVI ICSAVNAAG SASTRTRLT VTSREEHPP PVIIRGPVN QTLPINSVA FLSCEASGN PTPVISWYK ENHPVTSTN RINTTNPGL VEIHNLQKD DSGLYTCVA SSRSGKATW SGHLLVENP KNPNINFFK APEAIMLPG PPSRPHALN QSEGSVTIT WGQNNKIGS SSLLGYQIE LFGREEGVT PTWTVVARR VQGPTYTQH LLSPSVPYT FLVRAENGH GLGPPSQLS DIIIVGPDT AQNWGNPEV TVLSEARAS LVKGDIVRL SEAIPVLST AIKLVWEIL DAQYVEGLY IYYVTLDGP SDLPKPYSM LTVLHTGGA SSFTVNNLA KWSRYEFFL VPFYKTVEG QPSNSRTVR TLEDVPVEA PTSMEALLL NSTAVYLKW KPPPSETLN GDLQGYKIE IKANGTGTT DTVTVGTTP TLLLGNLTA GVAYTVKVA AMTRAGLGP FSSAAILRL DPASRHNDH HHQKPIDMQ SGDFITETW FMALLISMV SVMVLLFGA MLLVRRRQM LTKKTLTPS RSNGAVLTT PLASKTETP LWLDKDALP DYSNSLPEY SKLTPEVNL HLNPRPDHL DCSKYMQVE KEYASPNLA DYAEVEASQ VTSPAPYAT TTLVTGGRR MVWVEEPPY PSHNGGYYN RKVYSDSYF APNQTLRRK KSSNENPPD LVSPNQPVY ARVGPPGLS WRNATPSLT SFTPHKQVY HASTRSEPG NML
- >TC006743 RA
atgattttc ggacgattt ctggccggg gcgctgctt ttaacgcaa gctggagcc taccaccat gccagccaa caaccacct cgaatcacc gaacaccct gtagatacg acagtggcc cgacacgaa cctgccact ctcaattgt cacgcttcc ggtgaaccg gaaccgacc atcacgtgg tttaaagat ggaatgacg ttgagaacg gcgccacag gacgctaga tcgcatcgg gtgctgcta ccagcaggg aatttgttc tttttaagg gtggtacag agcaggaag gaaagtgat gctggggtg tactggtgc gaggctagc aatgctctg gggaaggct aggagcagg aatgccacc cttactgtc gcagtttta cgagacgaa ttcagactg gagccccag agcaccagg gtggcagca ggagaggat atcgtgatg gaatgtgcg cctccaagg ggcacaccc gagccccag gtcttctgg cgaaaggat gggcacacg ctggaggtg gaagggagg ctgaaactg gtcgatggg tcgaatctc gccatcaca gacaccaaa ccatcggat gatgggcga tatcagtgt gtggccaag aacacagcg ggtgtgagg gagtcggca gtggcgata ttgaaagtc tatgtgaaa ccatttctg atcaaaccc cccgaggac aaaacggcc ctcgtgggc tccaccgtg gacttcccg tgcgccgtg ggcggcgac cccctcccg gacgtcctc tggcgtcgc aacgccgcc ggcggcacc atgccccta ggccgcgtc cgcgtcctg gaagaccgc acccttcgc ctcgaaaga atcaccctt ttggaccaa gggcgctac acctgcgaa gccgacaac tacgccggt gccatcacc gccaccgcc ctcctcacc gtgcacgcg cccccaacc ctgaccacc cgccccctg gcccaaact gttgaatcc aaccgggac gtctccttc cactgcaac gtggaagga agccccaaa ccgttcatc ttctggtca ttcgaaggc gaccgcacc ctaatctac cccggaacc tccaccggc aactaccaa gtcaccaca gttgagggc aaatcaacc ctgacttta aaacaagcg caaccgcaa aactccgga acggttata atctgctcg gcggttaat gccgccggt tcggcctcg acccggacc cggttaacg gtcacgtcc cgtgaggag cacccgcct ccggttata atccggggg ccggttaac caaaccctc cccattaac tccgtggcg tttctctcg tgcgaagcc agcgggaac ccgactccg gtcatttcc tggtacaag gagaatcat ccggttacg tccacgaac cggattaat acaactaac cccggactt gtggaaatc cacaacttg caaaaagac gattccggg ttgtacaca tgtgtggcg tcttcacgc agcgggaaa gccacatgg agtggacat ttacttgtg gaaaacccc aaaaatccg aacattaat ttcttcaaa gccccggaa gctatcatg cttccgggg cctccaagt cgtccacac gccctcaac caatcggag ggcagtgtc acgatcacg tggggtcag aataacaaa atcgggtcg tccagcctg cttggttac caaatcgag ttgtttggg cgtgaggag ggggttacc cccacctgg actgtggta gcaaggcgg gtccaagga cctacatat acccaacat ttgctgagt ccttcagta ccttatacg ttcttggta cgggcagag aatgggcat gggttggga ccgccgtcc caactatcc gacataatc atagtaggt cctgacact gcccaaaat tggggaaac cccgaggtg acggttttg agcgaagcg agggctagt cttgtcaaa ggcgatatc gtgcggttg agcgaagcg attcctgtt ttatcgacc gcgatcaaa ctagtgtgg gaaatactg gatgctcag tacgttgaa gggctttac atttattat gtcacacta gacggaccc tccgacctt cccaagccc tacagcatg ttgacggtt ttacacacc ggaggtgcg tccagtttc actgtcaat aacctagcc aagtggtca cgttatgaa ttcttcctt gtgcctttt tacaagact gtcgaagga caaccttcc aattctcga actgtccga accttagaa gacgtgcct gttgaagct ccaacctcc atggaagct ttactccta aactcgacc gcggtttac ttgaaatgg aagccacca ccgagcgaa accctcaat ggggacctc caaggctac aaaatcgag atcaaagcc aacgggacc ggaaccacc gacactgta acagtcggc acaaccccc accctcctc ctggggaat ctaacggcc ggggttgcc tacaccgta aaagtggca gcaatgaca cgcgccggc ctcggcccc ttcagctcg gctgccatc ctccggctg gacccagcc tcccgtcac aacgaccac caccaccag aaacccatc gacatgcaa tctggggat ttcatcacc gagacctgg ttcatggcc ctcctgatc agcatggtc tcggtgatg gtactcctg ttcggcgcc atgttgcta gtgcggcgc cgccaaatg ctaacgaaa aaaacccta actccaagc cgctccaac ggcgcagtc ctaaccacg cccctcgct tccaagacc gaaaccccg ctgtggctg gacaaggac gcgctgccc gactactcc aactccctc cccgagtac tcgaagctc acccccgag gtcaacctc cacctcaac ccgcggcca gaccacctg gactgctcc aagtacatg caggtggag aaggagtac gctagtccg aacctggcc gattacgcc gaagtcgag gcgagtcag gtgacctcc ccggcgccc tacgccacc acgactctg gtgacgggc ggccgcagg atggtctgg gtcgaggag ccgccgtac ccctcgcac aacggcggc tattacaac cgaaaagtc tactcggat tcgtatttc gcgccaaat caaacgcta cgtcgcaag aaatcgtcc aatgagaac ccacccgat ctcgtgtcg cccaatcag ccggtgtat gcgcgcgtc ggcccgccc ggcctgtca tggcgcaac gccacgcct tctttgact agttttacg ccacataaa caggtctat catgcctcc acgaggagt gagcctggc aatatgttg tga
- >TC006743 RA
gaggggatg aagatgttt tcgggggtg gttgtactc gactacgtg tgagtgcgc cgtcagtcc gccgccggc gtcagaggc accaggacg ccgcttttc cactccaca aacaatcat taaacaatc tagcacaaa accgcgtgt gacaataaa atgtgacaa aaacgactc aattaaaag tgctcggac gtttatttt taatcccgt gtttaatta aactcgaaa atgagactg tgtgggact tgcgtgctg cgtgatgct taatctaat cgaaaaaca ctgtgatta gttgttaac ataaatgtt tgcggttgc cggaatgat tttcggacg atttctggc cggggcgct gcttttaac gcaagctgg agcctacca ccatgccag ccaacaacc acctcgaat caccgaaca ccctgtaga tacgacagt ggcccgaca cgaacctgc cactctcaa ttgtcacgc ttccggtga accggaacc gaccatcac gtggtttaa agatggaat gacgttgag aacggcgcc acaggacgc tagatcgca tcgggtgct gctaccagc agggaattt gttcttttt aagggtggt acagagcag gaaggaaag tgatgctgg ggtgtactg gtgcgaggc tagcaatgc tctggggaa ggctaggag caggaatgc cacccttac tgtcgcagt tttacgaga cgaattcag actggagcc ccagagcac cagggtggc agcaggaga ggatatcgt gatggaatg tgcgcctcc aaggggcac acccgagcc ccaggtctt ctggcgaaa ggatgggca cacgctgga ggtggaagg gaggctgaa actggtcga tgggtcgaa tctcgccat cacagacac caaaccatc ggatgatgg gcgatatca gtgtgtggc caagaacac agcgggtgt gagggagtc ggcagtggc gatattgaa agtctatgt gaaaccatt tctgatcaa accccccga ggacaaaac ggccctcgt gggctccac cgtggactt cccgtgcgc cgtgggcgg cgaccccct cccggacgt cctctggcg tcgcaacgc cgccggcgg caccatgcc cctaggccg cgtccgcgt cctggaaga ccgcaccct tcgcctcga aagaatcac ccttttgga ccaagggcg ctacacctg cgaagccga caactacgc cggtgccat caccgccac cgccctcct caccgtgca cgcgccccc aaccctgac cacccgccc cctggccca aactgttga atccaaccg ggacgtctc cttccactg caacgtgga aggaagccc caaaccgtt catcttctg gtcattcga aggcgaccg caccctaat ctaccccgg aacctccac cggcaacta ccaagtcac cacagttga gggcaaatc aaccctgac tttaaaaca agcgcaacc gcaaaactc cggaacggt tataatctg ctcggcggt taatgccgc cggttcggc ctcgacccg gacccggtt aacggtcac gtcccgtga ggagcaccc gcctccggt tataatccg ggggccggt taaccaaac cctccccat taactccgt ggcgtttct ctcgtgcga agccagcgg gaacccgac tccggtcat ttcctggta caaggagaa tcatccggt tacgtccac gaaccggat taatacaac taaccccgg acttgtgga aatccacaa cttgcaaaa agacgattc cgggttgta cacatgtgt ggcgtcttc acgcagcgg gaaagccac atggagtgg acatttact tgtggaaaa ccccaaaaa tccgaacat taatttctt caaagcccc ggaagctat catgcttcc ggggcctcc aagtcgtcc acacgccct caaccaatc ggagggcag tgtcacgat cacgtgggg tcagaataa caaaatcgg gtcgtccag cctgcttgg ttaccaaat cgagttgtt tgggcgtga ggagggggt tacccccac ctggactgt ggtagcaag gcgggtcca aggacctac atataccca acatttgct gagtccttc agtacctta tacgttctt ggtacgggc agagaatgg gcatgggtt gggaccgcc gtcccaact atccgacat aatcatagt aggtcctga cactgccca aaattgggg aaaccccga ggtgacggt tttgagcga agcgagggc tagtcttgt caaaggcga tatcgtgcg gttgagcga agcgattcc tgttttatc gaccgcgat caaactagt gtgggaaat actggatgc tcagtacgt tgaagggct ttacattta ttatgtcac actagacgg accctccga ccttcccaa gccctacag catgttgac ggttttaca caccggagg tgcgtccag tttcactgt caataacct agccaagtg gtcacgtta tgaattctt ccttgtgcc tttttacaa gactgtcga aggacaacc ttccaattc tcgaactgt ccgaacctt agaagacgt gcctgttga agctccaac ctccatgga agctttact cctaaactc gaccgcggt ttacttgaa atggaagcc accaccgag cgaaaccct caatgggga cctccaagg ctacaaaat cgagatcaa agccaacgg gaccggaac caccgacac tgtaacagt cggcacaac ccccaccct cctcctggg gaatctaac ggccggggt tgcctacac cgtaaaagt ggcagcaat gacacgcgc cggcctcgg ccccttcag ctcggctgc catcctccg gctggaccc agcctcccg tcacaacga ccaccacca ccagaaacc catcgacat gcaatctgg ggatttcat caccgagac ctggttcat ggccctcct gatcagcat ggtctcggt gatggtact cctgttcgg cgccatgtt gctagtgcg gcgccgcca aatgctaac gaaaaaaac cctaactcc aagccgctc caacggcgc agtcctaac cacgcccct cgcttccaa gaccgaaac cccgctgtg gctggacaa ggacgcgct gcccgacta ctccaactc cctccccga gtactcgaa gctcacccc cgaggtcaa cctccacct caacccgcg gccagacca cctggactg ctccaagta catgcaggt ggagaagga gtacgctag tccgaacct ggccgatta cgccgaagt cgaggcgag tcaggtgac ctccccggc gccctacgc caccacgac tctggtgac gggcggccg caggatggt ctgggtcga ggagccgcc gtacccctc gcacaacgg cggctatta caaccgaaa agtctactc ggattcgta tttcgcgcc aaatcaaac gctacgtcg caagaaatc gtccaatga gaacccacc cgatctcgt gtcgcccaa tcagccggt gtatgcgcg cgtcggccc gcccggcct gtcatggcg caacgccac gccttcttt gactagttt tacgccaca taaacaggt ctatcatgc ctccacgag gagtgagcc tggcaatat gttgtgaat ttgagtgat tttgttatc agtattact tgttaatgt ttttgggag agtgtctag tcagtgggc tcggttcaa atcgtccat ttttaagct tggttctac tagttaaac ccgtaacac tgtaatcct catcgatat tcaaattgt ataaaacat atcaaggag ttatgtata aattgtgac tggttgtat atttcttaa ctagggtag gttttttca gtgcgactg ggaaacggg ggacgaggt ggcaacacc gcctggcgc tcacgcgcc ctaacgacc gctaacgcg cctcgttaa ttgcaaaat acaagtaat aagtacccg aaaattatg tgcaaggcc gcttgcaca tatttagcc caccgcgga ttgaaaatt ccgggtggg tttgtgcag tgttgccac gtttttcgc cggaatagg gacagtttt ttgatttcc gtgtcttcc gttaatttc tcgattggt tgatgtaag aaacatgct cattatatt tttataaat aacgtgcca ttgtatatg ttataccat gtatgtaca gtatttaaa agtaataaa atttttaaa tgcgt
