Status
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Gene information
General information
TC006743 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13623659..13678550
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006743
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06385

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006743 RA
      MIFGRFLAG ALLLTQAGA YHHASQQPP RITEHPVDT 
      TVARHEPAT LNCHASGEP EPTITWFKD GMTLRTAPQ 
      DARSHRVLL PAGNLFFLR VVQSRKESD AGVYWCEAS 
      NALGKARSR NATLTVAVL RDEFRLEPQ STRVAAGED 
      IVMECAPPR GTPEPQVFW RKDGHTLEV EGRLKLVDG 
      SNLAITDTK PSDDGRYQC VAKNTAGVR ESAVAILKV 
      YVKPFLIKP PEDKTALVG STVDFPCAV GGDPLPDVL 
      WRRNAAGGT MPLGRVRVL EDRTLRLER ITLLDQGRY 
      TCEADNYAG AITATALLT VHAPPTLTT RPLAQTVES 
      NRDVSFHCN VEGSPKPFI FWSFEGDRT LIYPGTSTG 
      NYQVTTVEG KSTLTLKQA QPQNSGTVI ICSAVNAAG 
      SASTRTRLT VTSREEHPP PVIIRGPVN QTLPINSVA 
      FLSCEASGN PTPVISWYK ENHPVTSTN RINTTNPGL 
      VEIHNLQKD DSGLYTCVA SSRSGKATW SGHLLVENP 
      KNPNINFFK APEAIMLPG PPSRPHALN QSEGSVTIT 
      WGQNNKIGS SSLLGYQIE LFGREEGVT PTWTVVARR 
      VQGPTYTQH LLSPSVPYT FLVRAENGH GLGPPSQLS 
      DIIIVGPDT AQNWGNPEV TVLSEARAS LVKGDIVRL 
      SEAIPVLST AIKLVWEIL DAQYVEGLY IYYVTLDGP 
      SDLPKPYSM LTVLHTGGA SSFTVNNLA KWSRYEFFL 
      VPFYKTVEG QPSNSRTVR TLEDVPVEA PTSMEALLL 
      NSTAVYLKW KPPPSETLN GDLQGYKIE IKANGTGTT 
      DTVTVGTTP TLLLGNLTA GVAYTVKVA AMTRAGLGP 
      FSSAAILRL DPASRHNDH HHQKPIDMQ SGDFITETW 
      FMALLISMV SVMVLLFGA MLLVRRRQM LTKKTLTPS 
      RSNGAVLTT PLASKTETP LWLDKDALP DYSNSLPEY 
      SKLTPEVNL HLNPRPDHL DCSKYMQVE KEYASPNLA 
      DYAEVEASQ VTSPAPYAT TTLVTGGRR MVWVEEPPY 
      PSHNGGYYN RKVYSDSYF APNQTLRRK KSSNENPPD 
      LVSPNQPVY ARVGPPGLS WRNATPSLT SFTPHKQVY 
      HASTRSEPG NML
    CDS sequence
    • >TC006743 RA
      atgattttc ggacgattt ctggccggg gcgctgctt 
      ttaacgcaa gctggagcc taccaccat gccagccaa 
      caaccacct cgaatcacc gaacaccct gtagatacg 
      acagtggcc cgacacgaa cctgccact ctcaattgt 
      cacgcttcc ggtgaaccg gaaccgacc atcacgtgg 
      tttaaagat ggaatgacg ttgagaacg gcgccacag 
      gacgctaga tcgcatcgg gtgctgcta ccagcaggg 
      aatttgttc tttttaagg gtggtacag agcaggaag 
      gaaagtgat gctggggtg tactggtgc gaggctagc 
      aatgctctg gggaaggct aggagcagg aatgccacc 
      cttactgtc gcagtttta cgagacgaa ttcagactg 
      gagccccag agcaccagg gtggcagca ggagaggat 
      atcgtgatg gaatgtgcg cctccaagg ggcacaccc 
      gagccccag gtcttctgg cgaaaggat gggcacacg 
      ctggaggtg gaagggagg ctgaaactg gtcgatggg 
      tcgaatctc gccatcaca gacaccaaa ccatcggat 
      gatgggcga tatcagtgt gtggccaag aacacagcg 
      ggtgtgagg gagtcggca gtggcgata ttgaaagtc 
      tatgtgaaa ccatttctg atcaaaccc cccgaggac 
      aaaacggcc ctcgtgggc tccaccgtg gacttcccg 
      tgcgccgtg ggcggcgac cccctcccg gacgtcctc 
      tggcgtcgc aacgccgcc ggcggcacc atgccccta 
      ggccgcgtc cgcgtcctg gaagaccgc acccttcgc 
      ctcgaaaga atcaccctt ttggaccaa gggcgctac 
      acctgcgaa gccgacaac tacgccggt gccatcacc 
      gccaccgcc ctcctcacc gtgcacgcg cccccaacc 
      ctgaccacc cgccccctg gcccaaact gttgaatcc 
      aaccgggac gtctccttc cactgcaac gtggaagga 
      agccccaaa ccgttcatc ttctggtca ttcgaaggc 
      gaccgcacc ctaatctac cccggaacc tccaccggc 
      aactaccaa gtcaccaca gttgagggc aaatcaacc 
      ctgacttta aaacaagcg caaccgcaa aactccgga 
      acggttata atctgctcg gcggttaat gccgccggt 
      tcggcctcg acccggacc cggttaacg gtcacgtcc 
      cgtgaggag cacccgcct ccggttata atccggggg 
      ccggttaac caaaccctc cccattaac tccgtggcg 
      tttctctcg tgcgaagcc agcgggaac ccgactccg 
      gtcatttcc tggtacaag gagaatcat ccggttacg 
      tccacgaac cggattaat acaactaac cccggactt 
      gtggaaatc cacaacttg caaaaagac gattccggg 
      ttgtacaca tgtgtggcg tcttcacgc agcgggaaa 
      gccacatgg agtggacat ttacttgtg gaaaacccc 
      aaaaatccg aacattaat ttcttcaaa gccccggaa 
      gctatcatg cttccgggg cctccaagt cgtccacac 
      gccctcaac caatcggag ggcagtgtc acgatcacg 
      tggggtcag aataacaaa atcgggtcg tccagcctg 
      cttggttac caaatcgag ttgtttggg cgtgaggag 
      ggggttacc cccacctgg actgtggta gcaaggcgg 
      gtccaagga cctacatat acccaacat ttgctgagt 
      ccttcagta ccttatacg ttcttggta cgggcagag 
      aatgggcat gggttggga ccgccgtcc caactatcc 
      gacataatc atagtaggt cctgacact gcccaaaat 
      tggggaaac cccgaggtg acggttttg agcgaagcg 
      agggctagt cttgtcaaa ggcgatatc gtgcggttg 
      agcgaagcg attcctgtt ttatcgacc gcgatcaaa 
      ctagtgtgg gaaatactg gatgctcag tacgttgaa 
      gggctttac atttattat gtcacacta gacggaccc 
      tccgacctt cccaagccc tacagcatg ttgacggtt 
      ttacacacc ggaggtgcg tccagtttc actgtcaat 
      aacctagcc aagtggtca cgttatgaa ttcttcctt 
      gtgcctttt tacaagact gtcgaagga caaccttcc 
      aattctcga actgtccga accttagaa gacgtgcct 
      gttgaagct ccaacctcc atggaagct ttactccta 
      aactcgacc gcggtttac ttgaaatgg aagccacca 
      ccgagcgaa accctcaat ggggacctc caaggctac 
      aaaatcgag atcaaagcc aacgggacc ggaaccacc 
      gacactgta acagtcggc acaaccccc accctcctc 
      ctggggaat ctaacggcc ggggttgcc tacaccgta 
      aaagtggca gcaatgaca cgcgccggc ctcggcccc 
      ttcagctcg gctgccatc ctccggctg gacccagcc 
      tcccgtcac aacgaccac caccaccag aaacccatc 
      gacatgcaa tctggggat ttcatcacc gagacctgg 
      ttcatggcc ctcctgatc agcatggtc tcggtgatg 
      gtactcctg ttcggcgcc atgttgcta gtgcggcgc 
      cgccaaatg ctaacgaaa aaaacccta actccaagc 
      cgctccaac ggcgcagtc ctaaccacg cccctcgct 
      tccaagacc gaaaccccg ctgtggctg gacaaggac 
      gcgctgccc gactactcc aactccctc cccgagtac 
      tcgaagctc acccccgag gtcaacctc cacctcaac 
      ccgcggcca gaccacctg gactgctcc aagtacatg 
      caggtggag aaggagtac gctagtccg aacctggcc 
      gattacgcc gaagtcgag gcgagtcag gtgacctcc 
      ccggcgccc tacgccacc acgactctg gtgacgggc 
      ggccgcagg atggtctgg gtcgaggag ccgccgtac 
      ccctcgcac aacggcggc tattacaac cgaaaagtc 
      tactcggat tcgtatttc gcgccaaat caaacgcta 
      cgtcgcaag aaatcgtcc aatgagaac ccacccgat 
      ctcgtgtcg cccaatcag ccggtgtat gcgcgcgtc 
      ggcccgccc ggcctgtca tggcgcaac gccacgcct 
      tctttgact agttttacg ccacataaa caggtctat 
      catgcctcc acgaggagt gagcctggc aatatgttg 
      tga
    mRNA sequence
    • >TC006743 RA
      gaggggatg aagatgttt tcgggggtg gttgtactc 
      gactacgtg tgagtgcgc cgtcagtcc gccgccggc 
      gtcagaggc accaggacg ccgcttttc cactccaca 
      aacaatcat taaacaatc tagcacaaa accgcgtgt 
      gacaataaa atgtgacaa aaacgactc aattaaaag 
      tgctcggac gtttatttt taatcccgt gtttaatta 
      aactcgaaa atgagactg tgtgggact tgcgtgctg 
      cgtgatgct taatctaat cgaaaaaca ctgtgatta 
      gttgttaac ataaatgtt tgcggttgc cggaatgat 
      tttcggacg atttctggc cggggcgct gcttttaac 
      gcaagctgg agcctacca ccatgccag ccaacaacc 
      acctcgaat caccgaaca ccctgtaga tacgacagt 
      ggcccgaca cgaacctgc cactctcaa ttgtcacgc 
      ttccggtga accggaacc gaccatcac gtggtttaa 
      agatggaat gacgttgag aacggcgcc acaggacgc 
      tagatcgca tcgggtgct gctaccagc agggaattt 
      gttcttttt aagggtggt acagagcag gaaggaaag 
      tgatgctgg ggtgtactg gtgcgaggc tagcaatgc 
      tctggggaa ggctaggag caggaatgc cacccttac 
      tgtcgcagt tttacgaga cgaattcag actggagcc 
      ccagagcac cagggtggc agcaggaga ggatatcgt 
      gatggaatg tgcgcctcc aaggggcac acccgagcc 
      ccaggtctt ctggcgaaa ggatgggca cacgctgga 
      ggtggaagg gaggctgaa actggtcga tgggtcgaa 
      tctcgccat cacagacac caaaccatc ggatgatgg 
      gcgatatca gtgtgtggc caagaacac agcgggtgt 
      gagggagtc ggcagtggc gatattgaa agtctatgt 
      gaaaccatt tctgatcaa accccccga ggacaaaac 
      ggccctcgt gggctccac cgtggactt cccgtgcgc 
      cgtgggcgg cgaccccct cccggacgt cctctggcg 
      tcgcaacgc cgccggcgg caccatgcc cctaggccg 
      cgtccgcgt cctggaaga ccgcaccct tcgcctcga 
      aagaatcac ccttttgga ccaagggcg ctacacctg 
      cgaagccga caactacgc cggtgccat caccgccac 
      cgccctcct caccgtgca cgcgccccc aaccctgac 
      cacccgccc cctggccca aactgttga atccaaccg 
      ggacgtctc cttccactg caacgtgga aggaagccc 
      caaaccgtt catcttctg gtcattcga aggcgaccg 
      caccctaat ctaccccgg aacctccac cggcaacta 
      ccaagtcac cacagttga gggcaaatc aaccctgac 
      tttaaaaca agcgcaacc gcaaaactc cggaacggt 
      tataatctg ctcggcggt taatgccgc cggttcggc 
      ctcgacccg gacccggtt aacggtcac gtcccgtga 
      ggagcaccc gcctccggt tataatccg ggggccggt 
      taaccaaac cctccccat taactccgt ggcgtttct 
      ctcgtgcga agccagcgg gaacccgac tccggtcat 
      ttcctggta caaggagaa tcatccggt tacgtccac 
      gaaccggat taatacaac taaccccgg acttgtgga 
      aatccacaa cttgcaaaa agacgattc cgggttgta 
      cacatgtgt ggcgtcttc acgcagcgg gaaagccac 
      atggagtgg acatttact tgtggaaaa ccccaaaaa 
      tccgaacat taatttctt caaagcccc ggaagctat 
      catgcttcc ggggcctcc aagtcgtcc acacgccct 
      caaccaatc ggagggcag tgtcacgat cacgtgggg 
      tcagaataa caaaatcgg gtcgtccag cctgcttgg 
      ttaccaaat cgagttgtt tgggcgtga ggagggggt 
      tacccccac ctggactgt ggtagcaag gcgggtcca 
      aggacctac atataccca acatttgct gagtccttc 
      agtacctta tacgttctt ggtacgggc agagaatgg 
      gcatgggtt gggaccgcc gtcccaact atccgacat 
      aatcatagt aggtcctga cactgccca aaattgggg 
      aaaccccga ggtgacggt tttgagcga agcgagggc 
      tagtcttgt caaaggcga tatcgtgcg gttgagcga 
      agcgattcc tgttttatc gaccgcgat caaactagt 
      gtgggaaat actggatgc tcagtacgt tgaagggct 
      ttacattta ttatgtcac actagacgg accctccga 
      ccttcccaa gccctacag catgttgac ggttttaca 
      caccggagg tgcgtccag tttcactgt caataacct 
      agccaagtg gtcacgtta tgaattctt ccttgtgcc 
      tttttacaa gactgtcga aggacaacc ttccaattc 
      tcgaactgt ccgaacctt agaagacgt gcctgttga 
      agctccaac ctccatgga agctttact cctaaactc 
      gaccgcggt ttacttgaa atggaagcc accaccgag 
      cgaaaccct caatgggga cctccaagg ctacaaaat 
      cgagatcaa agccaacgg gaccggaac caccgacac 
      tgtaacagt cggcacaac ccccaccct cctcctggg 
      gaatctaac ggccggggt tgcctacac cgtaaaagt 
      ggcagcaat gacacgcgc cggcctcgg ccccttcag 
      ctcggctgc catcctccg gctggaccc agcctcccg 
      tcacaacga ccaccacca ccagaaacc catcgacat 
      gcaatctgg ggatttcat caccgagac ctggttcat 
      ggccctcct gatcagcat ggtctcggt gatggtact 
      cctgttcgg cgccatgtt gctagtgcg gcgccgcca 
      aatgctaac gaaaaaaac cctaactcc aagccgctc 
      caacggcgc agtcctaac cacgcccct cgcttccaa 
      gaccgaaac cccgctgtg gctggacaa ggacgcgct 
      gcccgacta ctccaactc cctccccga gtactcgaa 
      gctcacccc cgaggtcaa cctccacct caacccgcg 
      gccagacca cctggactg ctccaagta catgcaggt 
      ggagaagga gtacgctag tccgaacct ggccgatta 
      cgccgaagt cgaggcgag tcaggtgac ctccccggc 
      gccctacgc caccacgac tctggtgac gggcggccg 
      caggatggt ctgggtcga ggagccgcc gtacccctc 
      gcacaacgg cggctatta caaccgaaa agtctactc 
      ggattcgta tttcgcgcc aaatcaaac gctacgtcg 
      caagaaatc gtccaatga gaacccacc cgatctcgt 
      gtcgcccaa tcagccggt gtatgcgcg cgtcggccc 
      gcccggcct gtcatggcg caacgccac gccttcttt 
      gactagttt tacgccaca taaacaggt ctatcatgc 
      ctccacgag gagtgagcc tggcaatat gttgtgaat 
      ttgagtgat tttgttatc agtattact tgttaatgt 
      ttttgggag agtgtctag tcagtgggc tcggttcaa 
      atcgtccat ttttaagct tggttctac tagttaaac 
      ccgtaacac tgtaatcct catcgatat tcaaattgt 
      ataaaacat atcaaggag ttatgtata aattgtgac 
      tggttgtat atttcttaa ctagggtag gttttttca 
      gtgcgactg ggaaacggg ggacgaggt ggcaacacc 
      gcctggcgc tcacgcgcc ctaacgacc gctaacgcg 
      cctcgttaa ttgcaaaat acaagtaat aagtacccg 
      aaaattatg tgcaaggcc gcttgcaca tatttagcc 
      caccgcgga ttgaaaatt ccgggtggg tttgtgcag 
      tgttgccac gtttttcgc cggaatagg gacagtttt 
      ttgatttcc gtgtcttcc gttaatttc tcgattggt 
      tgatgtaag aaacatgct cattatatt tttataaat 
      aacgtgcca ttgtatatg ttataccat gtatgtaca 
      gtatttaaa agtaataaa atttttaaa tgcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY