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Gene information
General information
TC006719 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13336268..13340487
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006719
    iBeetle screen iB_06381

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006719 RA
      MSLIEDAVE AAKKAVQFD QEGKTEASI YYYEAASAL 
      LERAATSLD SDKAKSFKT KSNEYKNRA LELQNARNT 
      DHKVESDTN KQQLQKGYF LLQEAIEED ESGDKSDAI 
      ELYAQAIEF ITKNPDLMQ GELKQLALQ ALERAEALK 
      GIKHEPKVG RTPVRAKPD LHRGSSAHL QVTGADSYT 
      PEEKAVLCH TSNINSREY VPFMTVDLT EKFQYSIPF 
      TDKDGFLAL SPKQKREFS SWVRPQDLC TEPCIVDGA 
      APNYLNIKQ TVISDCSFV ASLAVSALY EKKFGRKLV 
      TAIIYPQRR DKQPRYNPF GKYMIKLHV NGVARKVII 
      DDYLPINRY GQLLCSYSS NKREFWISL LEKAYMKVM 
      GGYDFPGSN SNIDLHALT GWIPERTAI RKNDPDFNK 
      DALFSTLES RLAKGDVLV TVATGELSD ADAERSGLV 
      PTHAYAVMD VRTVDGVRL LKLKNPWSH LRWRGRYSE 
      LDIKRWTPE LQRELQYDP SGAAQFDNG VFWIDYESL 
      CAFFDVFYM NWNPELFKY TFCIHQSWN AGTGPVKDL 
      YTIGSNPQF SLDVSSNAG AVWLLLTRH ITDIDDFRE 
      NQEYITVLV YKNDGKRVY YPYDPPPYI DGVKINSPH 
      YLCKIILSP GSSTKYTIV ISQYEKTST IYYTLRAYA 
      TCPFTLRKI ADPFKYEQE ITGEWRGLT AGGCPNHPL 
      TFKNNPKFR LEIEGSSNR VFIELKGPK QYQIGIEVV 
      ILKVSDDSV TAPFRSKSS GAYRSGYVI LELENIPAG 
      VLQIVPSTF LPSQEGPFF LLVKASAPI QLTKF
    CDS sequence
    • >TC006719 RA
      atgtctcta atcgaagac gcggtcgaa gcagccaaa 
      aaagccgtc cagttcgac caagagggc aaaaccgaa 
      gcttcgatc tactactac gaagccgcc tcagcgctc 
      cttgaacgc gccgcaact tcgcttgac tcagacaag 
      gcaaaatcg ttcaaaacc aaatcaaac gagtacaaa 
      aaccgggca cttgagctc caaaatgcc cgaaacacc 
      gaccacaag gtggaaagc gacaccaac aagcaacaa 
      ctccagaaa ggctacttc ctcctccag gaggccatc 
      gaagaggac gaatccggg gacaagtcc gacgcgatc 
      gaactctac gcccaagcc atcgaattc atcaccaaa 
      aaccccgac ttgatgcaa ggcgagctc aagcaactg 
      gccctccag gccctcgaa cgcgccgag gccctcaaa 
      ggcatcaag cacgagcca aaagtgggc agaaccccc 
      gtgagggcg aagcccgac ttgcaccgc ggctccagc 
      gcgcatttg caggtcaca ggggccgac tcctacacg 
      ccggaggag aaggccgtc ctgtgccac acttccaac 
      atcaacagc agggaatat gtcccgttc atgaccgtt 
      gatctgacc gagaaattc cagtactcg attcctttc 
      actgacaaa gacggcttt ctggccctg tccccgaag 
      caaaaacgc gagttttcg agctgggtg cgcccacag 
      gacttgtgc acggagccc tgtattgtg gacggggcg 
      gcccccaat tatttgaat atcaaacaa acggttatt 
      tccgattgt tcgtttgtt gcgagtctc gcagtcagc 
      gccttgtat gagaaaaag ttcgggcgc aaattggtc 
      actgcgatt atttacccg caaaggagg gataaacag 
      ccgcgttac aaccccttt gggaagtac atgattaag 
      ctgcatgtt aatggggtt gcgagaaaa gttataatc 
      gatgactat ttgccgatt aatcggtat ggacagttg 
      ttgtgttcg tattcgagc aataagagg gaattctgg 
      atttcgctg cttgagaag gcttatatg aaggtgatg 
      ggagggtac gactttcca ggcagtaat agtaacatc 
      gacttgcac gcgctcacc gggtggatc cccgaacgc 
      acggcgatc cgtaaaaac gaccccgat ttcaacaaa 
      gacgccctt ttttcaacc cttgagtcg cgtttagct 
      aaaggcgac gttcttgtg actgtagcc acaggtgaa 
      ttatctgat gccgacgct gaacgtagc ggtctagtc 
      ccgacacat gcttacgcc gtcatggac gtccggacg 
      gtagacggg gtgcgcctc ctgaaactg aaaaatccc 
      tggtcgcac ctacggtgg cgtggccgt tactcggaa 
      ctggatatt aaacggtgg acccccgag ctacaaagg 
      gagctccag tatgaccca agcggggct gcgcagttt 
      gacaatggg gttttttgg atcgactat gagagtttg 
      tgtgcgttt ttcgatgtt ttttacatg aattggaac 
      ccagagttg ttcaaatat actttttgt atacaccag 
      tcatggaac gcgggcact gggcctgtc aaagacttg 
      tacacgatt ggctccaac ccgcagttt tccctcgac 
      gtgtcaagt aatgcgggg gctgtttgg ctgctccta 
      acccgtcat atcaccgat attgacgat tttagggaa 
      aatcaggaa tatataaca gttctggtg tacaaaaac 
      gacgggaaa cgggtgtat tacccgtac gacccgccc 
      ccgtatatc gacggcgtt aaaatcaac agcccccat 
      tatttgtgt aaaattata ctgagtccc ggcagtagc 
      accaaatac accattgtt atatcacag tatgagaaa 
      acttcgact atttactac actctgagg gcttatgcc 
      acgtgtccg ttcacattg cgcaaaatt gcggaccct 
      tttaaatac gaacaggag ataacgggc gaatggcgg 
      ggtctcacg gctgggggg tgccccaat caccccctc 
      accttcaaa aacaacccc aaatttcgc ctggaaatt 
      gaaggttca agtaatagg gtttttatc gagttgaag 
      ggccccaaa caataccag atcgggatc gaggttgtg 
      attttgaaa gtcagtgac gattctgtc acggcgccc 
      tttcgctca aagtcgtca ggggcttac aggtcgggt 
      tatgtgata ctagagctg gaaaacata ccggctggg 
      gtcctccag atcgtcccg tccacgttt ttaccatca 
      caggaaggc ccctttttc ctccttgtg aaagcgtcg 
      gcaccaatc cagctcacc aagttttaa
    mRNA sequence
    • >TC006719 RA
      acaaagaaa aaaaaagac caagaacta agtaaaatg 
      tcatgtcac ccagctggt gggttttcc agtaataaa 
      agacgatta tttttatga ctcgtgtat gtcattaat 
      tgaaatcga cccacacga cgacgacca aatacaacc 
      atcgcttat cagtcctta tcacgtctt acattaaca 
      ataaacaca acataaccc aactttcat ctgtcaatg 
      tcactcaat tgtaggata aatgtctct aatcgaaga 
      cgcggtcga agcagccaa aaaagccgt ccagttcga 
      ccaagaggg caaaaccga agcttcgat ctactacta 
      cgaagccgc ctcagcgct ccttgaacg cgccgcaac 
      ttcgcttga ctcagacaa ggcaaaatc gttcaaaac 
      caaatcaaa cgagtacaa aaaccgggc acttgagct 
      ccaaaatgc ccgaaacac cgaccacaa ggtggaaag 
      cgacaccaa caagcaaca actccagaa aggctactt 
      cctcctcca ggaggccat cgaagagga cgaatccgg 
      ggacaagtc cgacgcgat cgaactcta cgcccaagc 
      catcgaatt catcaccaa aaaccccga cttgatgca 
      aggcgagct caagcaact ggccctcca ggccctcga 
      acgcgccga ggccctcaa aggcatcaa gcacgagcc 
      aaaagtggg cagaacccc cgtgagggc gaagcccga 
      cttgcaccg cggctccag cgcgcattt gcaggtcac 
      aggggccga ctcctacac gccggagga gaaggccgt 
      cctgtgcca cacttccaa catcaacag cagggaata 
      tgtcccgtt catgaccgt tgatctgac cgagaaatt 
      ccagtactc gattccttt cactgacaa agacggctt 
      tctggccct gtccccgaa gcaaaaacg cgagttttc 
      gagctgggt gcgcccaca ggacttgtg cacggagcc 
      ctgtattgt ggacggggc ggcccccaa ttatttgaa 
      tatcaaaca aacggttat ttccgattg ttcgtttgt 
      tgcgagtct cgcagtcag cgccttgta tgagaaaaa 
      gttcgggcg caaattggt cactgcgat tatttaccc 
      gcaaaggag ggataaaca gccgcgtta caacccctt 
      tgggaagta catgattaa gctgcatgt taatggggt 
      tgcgagaaa agttataat cgatgacta tttgccgat 
      taatcggta tggacagtt gttgtgttc gtattcgag 
      caataagag ggaattctg gatttcgct gcttgagaa 
      ggcttatat gaaggtgat gggagggta cgactttcc 
      aggcagtaa tagtaacat cgacttgca cgcgctcac 
      cgggtggat ccccgaacg cacggcgat ccgtaaaaa 
      cgaccccga tttcaacaa agacgccct tttttcaac 
      ccttgagtc gcgtttagc taaaggcga cgttcttgt 
      gactgtagc cacaggtga attatctga tgccgacgc 
      tgaacgtag cggtctagt cccgacaca tgcttacgc 
      cgtcatgga cgtccggac ggtagacgg ggtgcgcct 
      cctgaaact gaaaaatcc ctggtcgca cctacggtg 
      gcgtggccg ttactcgga actggatat taaacggtg 
      gacccccga gctacaaag ggagctcca gtatgaccc 
      aagcggggc tgcgcagtt tgacaatgg ggttttttg 
      gatcgacta tgagagttt gtgtgcgtt tttcgatgt 
      tttttacat gaattggaa cccagagtt gttcaaata 
      tactttttg tatacacca gtcatggaa cgcgggcac 
      tgggcctgt caaagactt gtacacgat tggctccaa 
      cccgcagtt ttccctcga cgtgtcaag taatgcggg 
      ggctgtttg gctgctcct aacccgtca tatcaccga 
      tattgacga ttttaggga aaatcagga atatataac 
      agttctggt gtacaaaaa cgacgggaa acgggtgta 
      ttacccgta cgacccgcc cccgtatat cgacggcgt 
      taaaatcaa cagccccca ttatttgtg taaaattat 
      actgagtcc cggcagtag caccaaata caccattgt 
      tatatcaca gtatgagaa aacttcgac tatttacta 
      cactctgag ggcttatgc cacgtgtcc gttcacatt 
      gcgcaaaat tgcggaccc ttttaaata cgaacagga 
      gataacggg cgaatggcg gggtctcac ggctggggg 
      gtgccccaa tcaccccct caccttcaa aaacaaccc 
      caaatttcg cctggaaat tgaaggttc aagtaatag 
      ggtttttat cgagttgaa gggccccaa acaatacca 
      gatcgggat cgaggttgt gattttgaa agtcagtga 
      cgattctgt cacggcgcc ctttcgctc aaagtcgtc 
      aggggctta caggtcggg ttatgtgat actagagct 
      ggaaaacat accggctgg ggtcctcca gatcgtccc 
      gtccacgtt tttaccatc acaggaagg ccccttttt 
      cctccttgt gaaagcgtc ggcaccaat ccagctcac 
      caagtttta agtctatta cactgtgat ttaacacaa 
      atgcctttt ttattttgt ttacgtatt gtattatgc 
      tgataataa actatttct aaaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY