GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06378 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC000856 RA
MESALMEAG IIFLTIVSF VYLYFKHAY TYWKARNVP QLSPTFPFG DMASVIFRR HNMGDKIKE IYDETKARG HKYVGLYFF SRKAFLPID PVLIKKILA PDFQHFHDR GIYYDEEND PISAHLFSL AGPKWKNLR ARLTPAYSP GKLKFMFDT VLACGEEMA DILKEIAKR EGKVEIKEI LARYSTDVI GCCAFGIDC NSMRNPTAE FRLMGRRAF TQTIGDVLK MIVIRSFPR LAKLLGFGV FSPTVTGFF QRVVNETIR YREENNVTR NDFMQLLIQ LKNKGKLEG DGEEKDGAP AVGTSLTLD EAAAQAFIF FLAGFETTS TTISFALFE MARNESIRT KARTEINKV LGKHGGKLT YEATMEMEY LDTVISETL RKYPPAPVF LRKCTKTYR VPDTDVIIE EGLSVLIPA YGLHRDPEY FPDPETFDP DRFNEDNRS KIWDYTYIP FGDGPRICI GNY
- >TC000856 RA
atggaaagt gctctgatg gaggctgga attattttc ctgacgatc gtctccttt gtatactta tatttcaag cacgcctac acgtactgg aaagcccga aacgtcccc caactctcg cccacgttc ccttttggc gacatggcc agcgtcatt ttcagacgg cacaacatg ggagataaa atcaaagaa atttacgat gagacgaaa gcgcggggg cacaaatac gtcggattg tacttcttc agcaggaag gccttcctg ccgattgat ccggtcttg atcaagaaa attctcgcg ccggatttt cagcatttc cacgacagg ggaatctat tacgatgaa gagaatgat ccgataagt gcgcatctg ttctcgctc gctggaccc aaatggaag aatctgagg gccagatta acaccggct tactcgccg ggcaaattg aaatttatg ttcgatacg gtcctggcc tgtggggag gagatggcc gacattctt aaggagatc gcgaaacgc gaagggaag gtcgaaatc aaagagatt ctggcgaga tatagcacc gacgtaatc gggtgctgc gcctttggc atcgactgc aatagcatg cgtaaccca acggccgaa ttccgttta atgggccgt cgcgctttc acccaaaca atcggcgat gtgctcaaa atgatagtc atccgaagc tttcctcgt ttggctaag ctcctgggt ttcggtgta ttttcccct accgtcact ggtttcttt caacgggtc gtaaacgag acgattcgc tatcgcgaa gaaaacaac gtcactaga aacgatttc atgcagttg ctaattcaa ctcaagaac aaaggaaaa ttagaaggc gacggagaa gagaaagac ggggcgccg gccgtcggg acgtctttg acgctggac gaggccgca gcgcaagcc ttcattttc ttcttggct ggatttgag acgacgtcc acaacaatc agctttgcg ctgtttgag atggcccga aatgaaagc atacgcacc aaagcacgg accgaaatt aacaaagtg ctgggaaag cacggaggg aagctgact tatgaggcc acgatggaa atggaatat ttggacact gttatttct gaaacgtta cgtaaatat cccccagca ccggtgttt ttgcgaaaa tgtacgaaa acttatcgc gtgccagac acggatgtc ataatagaa gagggttta tcggtgttg atcccagct tatggatta cacagagat cctgaatat tttccagat cctgagacg ttcgatcct gacagattt aatgaagac aataggagc aaaatatgg gattatacc tacatccct ttcggtgac ggacctcgt atttgcatt ggtaattat tga
- >TC000856 RA
aattatggt agttttagt gttattgga ccattttta cgtcttctt ttacttgtt tttctacat ttttttcaa cgttgtgag ttacatgat ttacgaagg cgttaattt ggacgatgt ggctcgcta ttcttcgct ggtgaaaat cttcttgta aatatcgca gtgggaaac gtcttagtg aaagtggtt attaacgac gtaacccaa acacctcgc ggctgagtg aaattcggt tcatcgact ctaacaaac attaaagat ggaaagtgc tctgatgga ggctggaat tattttcct gacgatcgt ctcctttgt atacttata tttcaagca cgcctacac gtactggaa agcccgaaa cgtccccca actctcgcc cacgttccc ttttggcga catggccag cgtcatttt cagacggca caacatggg agataaaat caaagaaat ttacgatga gacgaaagc gcgggggca caaatacgt cggattgta cttcttcag caggaaggc cttcctgcc gattgatcc ggtcttgat caagaaaat tctcgcgcc ggattttca gcatttcca cgacagggg aatctatta cgatgaaga gaatgatcc gataagtgc gcatctgtt ctcgctcgc tggacccaa atggaagaa tctgagggc cagattaac accggctta ctcgccggg caaattgaa atttatgtt cgatacggt cctggcctg tggggagga gatggccga cattcttaa ggagatcgc gaaacgcga agggaaggt cgaaatcaa agagattct ggcgagata tagcaccga cgtaatcgg gtgctgcgc ctttggcat cgactgcaa tagcatgcg taacccaac ggccgaatt ccgtttaat gggccgtcg cgctttcac ccaaacaat cggcgatgt gctcaaaat gatagtcat ccgaagctt tcctcgttt ggctaagct cctgggttt cggtgtatt ttcccctac cgtcactgg tttctttca acgggtcgt aaacgagac gattcgcta tcgcgaaga aaacaacgt cactagaaa cgatttcat gcagttgct aattcaact caagaacaa aggaaaatt agaaggcga cggagaaga gaaagacgg ggcgccggc cgtcgggac gtctttgac gctggacga ggccgcagc gcaagcctt cattttctt cttggctgg atttgagac gacgtccac aacaatcag ctttgcgct gtttgagat ggcccgaaa tgaaagcat acgcaccaa agcacggac cgaaattaa caaagtgct gggaaagca cggagggaa gctgactta tgaggccac gatggaaat ggaatattt ggacactgt tatttctga aacgttacg taaatatcc cccagcacc ggtgttttt gcgaaaatg tacgaaaac ttatcgcgt gccagacac ggatgtcat aatagaaga gggtttatc ggtgttgat cccagctta tggattaca cagagatcc tgaatattt tccagatcc tgagacgtt cgatcctga cagatttaa tgaagacaa taggagcaa aatatggga ttataccta catcccttt cggtgacgg acctcgtat ttgcattgg taattattg agtcgatta taaagacat ggatgccaa attctttat tttcaatgg gtggacaag agccaataa agttacaga aataac
