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Gene information
General information
TC000856 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7047188..7052119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000856
    iBeetle screen iB_06378

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000856 RA
      MESALMEAG IIFLTIVSF VYLYFKHAY TYWKARNVP 
      QLSPTFPFG DMASVIFRR HNMGDKIKE IYDETKARG 
      HKYVGLYFF SRKAFLPID PVLIKKILA PDFQHFHDR 
      GIYYDEEND PISAHLFSL AGPKWKNLR ARLTPAYSP 
      GKLKFMFDT VLACGEEMA DILKEIAKR EGKVEIKEI 
      LARYSTDVI GCCAFGIDC NSMRNPTAE FRLMGRRAF 
      TQTIGDVLK MIVIRSFPR LAKLLGFGV FSPTVTGFF 
      QRVVNETIR YREENNVTR NDFMQLLIQ LKNKGKLEG 
      DGEEKDGAP AVGTSLTLD EAAAQAFIF FLAGFETTS 
      TTISFALFE MARNESIRT KARTEINKV LGKHGGKLT 
      YEATMEMEY LDTVISETL RKYPPAPVF LRKCTKTYR 
      VPDTDVIIE EGLSVLIPA YGLHRDPEY FPDPETFDP 
      DRFNEDNRS KIWDYTYIP FGDGPRICI GNY
    CDS sequence
    • >TC000856 RA
      atggaaagt gctctgatg gaggctgga attattttc 
      ctgacgatc gtctccttt gtatactta tatttcaag 
      cacgcctac acgtactgg aaagcccga aacgtcccc 
      caactctcg cccacgttc ccttttggc gacatggcc 
      agcgtcatt ttcagacgg cacaacatg ggagataaa 
      atcaaagaa atttacgat gagacgaaa gcgcggggg 
      cacaaatac gtcggattg tacttcttc agcaggaag 
      gccttcctg ccgattgat ccggtcttg atcaagaaa 
      attctcgcg ccggatttt cagcatttc cacgacagg 
      ggaatctat tacgatgaa gagaatgat ccgataagt 
      gcgcatctg ttctcgctc gctggaccc aaatggaag 
      aatctgagg gccagatta acaccggct tactcgccg 
      ggcaaattg aaatttatg ttcgatacg gtcctggcc 
      tgtggggag gagatggcc gacattctt aaggagatc 
      gcgaaacgc gaagggaag gtcgaaatc aaagagatt 
      ctggcgaga tatagcacc gacgtaatc gggtgctgc 
      gcctttggc atcgactgc aatagcatg cgtaaccca 
      acggccgaa ttccgttta atgggccgt cgcgctttc 
      acccaaaca atcggcgat gtgctcaaa atgatagtc 
      atccgaagc tttcctcgt ttggctaag ctcctgggt 
      ttcggtgta ttttcccct accgtcact ggtttcttt 
      caacgggtc gtaaacgag acgattcgc tatcgcgaa 
      gaaaacaac gtcactaga aacgatttc atgcagttg 
      ctaattcaa ctcaagaac aaaggaaaa ttagaaggc 
      gacggagaa gagaaagac ggggcgccg gccgtcggg 
      acgtctttg acgctggac gaggccgca gcgcaagcc 
      ttcattttc ttcttggct ggatttgag acgacgtcc 
      acaacaatc agctttgcg ctgtttgag atggcccga 
      aatgaaagc atacgcacc aaagcacgg accgaaatt 
      aacaaagtg ctgggaaag cacggaggg aagctgact 
      tatgaggcc acgatggaa atggaatat ttggacact 
      gttatttct gaaacgtta cgtaaatat cccccagca 
      ccggtgttt ttgcgaaaa tgtacgaaa acttatcgc 
      gtgccagac acggatgtc ataatagaa gagggttta 
      tcggtgttg atcccagct tatggatta cacagagat 
      cctgaatat tttccagat cctgagacg ttcgatcct 
      gacagattt aatgaagac aataggagc aaaatatgg 
      gattatacc tacatccct ttcggtgac ggacctcgt 
      atttgcatt ggtaattat tga
    mRNA sequence
    • >TC000856 RA
      aattatggt agttttagt gttattgga ccattttta 
      cgtcttctt ttacttgtt tttctacat ttttttcaa 
      cgttgtgag ttacatgat ttacgaagg cgttaattt 
      ggacgatgt ggctcgcta ttcttcgct ggtgaaaat 
      cttcttgta aatatcgca gtgggaaac gtcttagtg 
      aaagtggtt attaacgac gtaacccaa acacctcgc 
      ggctgagtg aaattcggt tcatcgact ctaacaaac 
      attaaagat ggaaagtgc tctgatgga ggctggaat 
      tattttcct gacgatcgt ctcctttgt atacttata 
      tttcaagca cgcctacac gtactggaa agcccgaaa 
      cgtccccca actctcgcc cacgttccc ttttggcga 
      catggccag cgtcatttt cagacggca caacatggg 
      agataaaat caaagaaat ttacgatga gacgaaagc 
      gcgggggca caaatacgt cggattgta cttcttcag 
      caggaaggc cttcctgcc gattgatcc ggtcttgat 
      caagaaaat tctcgcgcc ggattttca gcatttcca 
      cgacagggg aatctatta cgatgaaga gaatgatcc 
      gataagtgc gcatctgtt ctcgctcgc tggacccaa 
      atggaagaa tctgagggc cagattaac accggctta 
      ctcgccggg caaattgaa atttatgtt cgatacggt 
      cctggcctg tggggagga gatggccga cattcttaa 
      ggagatcgc gaaacgcga agggaaggt cgaaatcaa 
      agagattct ggcgagata tagcaccga cgtaatcgg 
      gtgctgcgc ctttggcat cgactgcaa tagcatgcg 
      taacccaac ggccgaatt ccgtttaat gggccgtcg 
      cgctttcac ccaaacaat cggcgatgt gctcaaaat 
      gatagtcat ccgaagctt tcctcgttt ggctaagct 
      cctgggttt cggtgtatt ttcccctac cgtcactgg 
      tttctttca acgggtcgt aaacgagac gattcgcta 
      tcgcgaaga aaacaacgt cactagaaa cgatttcat 
      gcagttgct aattcaact caagaacaa aggaaaatt 
      agaaggcga cggagaaga gaaagacgg ggcgccggc 
      cgtcgggac gtctttgac gctggacga ggccgcagc 
      gcaagcctt cattttctt cttggctgg atttgagac 
      gacgtccac aacaatcag ctttgcgct gtttgagat 
      ggcccgaaa tgaaagcat acgcaccaa agcacggac 
      cgaaattaa caaagtgct gggaaagca cggagggaa 
      gctgactta tgaggccac gatggaaat ggaatattt 
      ggacactgt tatttctga aacgttacg taaatatcc 
      cccagcacc ggtgttttt gcgaaaatg tacgaaaac 
      ttatcgcgt gccagacac ggatgtcat aatagaaga 
      gggtttatc ggtgttgat cccagctta tggattaca 
      cagagatcc tgaatattt tccagatcc tgagacgtt 
      cgatcctga cagatttaa tgaagacaa taggagcaa 
      aatatggga ttataccta catcccttt cggtgacgg 
      acctcgtat ttgcattgg taattattg agtcgatta 
      taaagacat ggatgccaa attctttat tttcaatgg 
      gtggacaag agccaataa agttacaga aataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY