Status
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Gene information
General information
TC006692 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12953484..12960902
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006692
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06376

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006692 RA
      MTLYFENPV SFPEPGSIS LNGVWHPNS ALLAVASFS 
      QDKGGYVTI FDELGEPLS DINYPVHRS FQVTALAWH 
      PERHVLATG WENGEFKIW NGEKDFALI GGPHKAPIT 
      LLEFSEKGG RLVSCDSAG SLVGWKVVD SQAQTVMVF 
      HLDLKESIT HLTFRITIK PHPDFDVEG LAKAAVNGD 
      ERALDLFSN WRPKTTARK FRVQEGSDN LSFYVGTQV 
      GSIYYVNSG GSCMEVLNT EGVPLSYIV HHPSKDALI 
      VMMEGLTIG HFSVDSQGQ LNELAKVKL SGRVQSRGV 
      GTQGLVWCS NSSLAVLTG DLIVRIWDI ETNDNYVLP 
      TSLKFYTND DKNRTVSEV FTCLAFCKL NQTLCAGTN 
      IGRIYFWTR NTNNGESPE DCWELNNVN TVSGTVKQL 
      MWGSVQLRL PLLSVNCVT CVYIMKEQS ICTCFSDEV 
      WCTQKTASQ VLVESAKSN VLLTLETQI TDMCINDSF 
      AIFTNGRRV LVYEIFWDG SDSEKFELN TNISKQTES 
      SADFSIKLI STFACDNEK VLLHKKMIV VLTAKAVTL 
      RQLNGSVIT TIVSPSHEG EPIGLDVTG NYLTIFTIE 
      GYMKVYELG ENEAKLSIP ARNLYDMCP DFGEIIQAK 
      SNSSGNKIA LTLAAANLV PDGRLYVWD VENDNLLSY 
      DFHKYGNFD LEPSPDEFS VDERSEQND SSNDDETVA 
      VFDEICSNR IPLHLFWDA HDPRLLICD AKKVKLGLG 
      EKKLKRSRS DTKTTLKDQ DHIIITMFV SSEHGIKMH 
      DIRGVEAES RLLALATPY LVILEKLVI VRDVMSDFN 
      GLESCNKET RDAVLNFSY NLSLGNMDE AFKAIKLVQ 
      NPGVWGSLA RMCVKTRRL DVAGVCLGH MGNARAAMA 
      LRVAVADYS LPHEAKLAV LAVHLGMLD EAEQLYIQC 
      QRYDLLNKL LRSRNKFDA AHAIAESQD RIHLKNTDH 
      AWGKALEAV GDFKEAAVR YEKANTHKF DVPRMLLDQ 
      PQQLETYMG KTKDPDLLK WWGQYVESQ GEMQAALKI 
      YSSAGDVYS QVRVLCFLG EESKAAELA RSGRDKAAF 
      YHMARFYET TGNFEEAVN FFTKANAYS NAVRLCKEN 
      HMSDELWNI GSVAGTREK LECARYFEE IGALDKSVI 
      LYHRAGMLH KALDLAFKA QQYDILQQI ATDLDADSD 
      PALVQKCAD YFVTNEQFD KAVDLLAIA KKYKEAITI 
      CLTHNVQLT EDLAEKLTP EKDQLEEAD RVNVLQTLA 
      ESLMLQGNY HLATKKFTQ AGDKIRAMK ALLKSGDTD 
      KIIFFAGVS RQREIYIMA ANYLQSLDW QNNPEILRN 
      IITFYSKGK ALDLLANFY VACAQVEID EFKNYEKAF 
      GALTEASRC LAKIGNPRD SSQHQRATE IVQQRLTMI 
      KRFVDIRRL FDRGDYQAG IAQCRQLLM TGGKELEES 
      VRRGDICAL MIQEYIKSG NFTEAKQLL GELKQLLFS 
      SGNTPITYY LNKETIEAL AHGLGVPVS TLIPAKMTL 
      EDEDTEEFV EEAIED
    CDS sequence
    • >TC006692 RA
      atgacactt tattttgaa aatccggtg agttttccg 
      gagccgggc tcgatttcc ctgaacggg gtctggcac 
      ccgaactcg gcccttctg gccgtggcc agtttttcg 
      caagataag ggcggttac gtgacgatt ttcgacgag 
      ttgggggag cctttgagc gatataaac taccccgta 
      caccgcagt tttcaagta acggctctt gcctggcac 
      cccgagcgc catgtcctg gccaccggg tgggaaaac 
      ggcgagttt aaaatctgg aacggggag aaggatttt 
      gcgctcatt gggggcccg cacaaggcc cccatcacg 
      ctgctggag ttcagcgaa aaggggggc cggcttgtg 
      tcgtgcgat tcggcgggg tcgcttgtg gggtggaag 
      gttgtggat agtcaggcg cagactgtt atggttttt 
      cacttggat ttgaaggag tcgattacg catttgacg 
      ttcaggatc acgataaag ccgcatccg gattttgat 
      gttgagggg ttggctaag gcggcggtt aatggggat 
      gaaagggcc ctcgatctt tttagtaat tggcgtccg 
      aagaccaca gcccggaaa ttccgtgtt caggagggt 
      tctgacaac ttgtcattt tacgtgggg acgcaagtg 
      ggctcaatt tactacgtt aatagcggg ggctcgtgc 
      atggaagtc ttaaacacg gaaggggtg cccctttct 
      tacattgtg caccatccg agcaaagac gccctaatt 
      gtcatgatg gagggttta acaatcggt catttctcc 
      gttgatagt caaggccag ttaaacgaa ttagctaaa 
      gttaaatta agcggaagg gtgcaaagt cgcggtgtt 
      ggaacacag ggcttagtc tggtgttcc aactccagt 
      ttggcggtt ttaaccggt gatttaatc gtccgcatt 
      tgggacatt gaaaccaac gataattac gtgttaccc 
      acgtcgttg aaattctac acaaacgac gataaaaat 
      cgcacagtt agtgaagtt ttcacgtgc ttggcgttc 
      tgcaaattg aaccaaact ctgtgtgcg ggcaccaac 
      attggtcgc atttatttc tggaccagg aacacaaac 
      aatggggaa agtcccgag gattgttgg gagttgaat 
      aacgtaaat acggtcagc ggaaccgtt aaacaatta 
      atgtggggt tctgttcag ttaagactc ccgttattg 
      agcgttaat tgcgtcacg tgtgtttac ataatgaag 
      gaacaatcg atttgtacg tgtttttcc gatgaggtt 
      tggtgcacg caaaaaacc gccagtcag gtgcttgtc 
      gaaagcgcc aaaagcaac gttttgctg actttggag 
      acacaaatc actgatatg tgtatcaat gattcgttt 
      gcaattttc acaaacggg agacgcgtt ctagtttat 
      gaaattttc tgggatggg tctgactcg gaaaagttc 
      gaactcaat acgaacatt tccaagcag accgaaagt 
      tcggccgat ttttcgatt aaactaatc agcacgttt 
      gcgtgtgac aatgagaag gttttactc cacaaaaaa 
      atgatcgtt gttttgacc gcgaaagcg gtcacactc 
      cgacaatta aacggttcg gttataacc accatcgtg 
      tcaccctca cacgagggc gaaccgatc ggactcgac 
      gtcacgggg aattacctc acgattttc acaatcgaa 
      ggctacatg aaagtgtac gaactgggc gagaatgag 
      gcgaaactg tcgattccg gcccggaat ttgtacgac 
      atgtgcccg gactttggc gaaataatc caggcgaaa 
      agcaactca agcggcaat aaaatcgcc ctgacttta 
      gccgcggcc aatctagtg cccgatggg cgcctgtac 
      gtctgggac gtggaaaat gacaatttg ctatcgtac 
      gatttccac aagtacggt aattttgat ttggagcca 
      agtccggac gaattcagc gtcgacgaa cgaagcgaa 
      caaaatgat tcgtcaaac gacgacgaa accgttgcg 
      gttttcgac gaaatttgc tcgaatcgc atccctttg 
      cacttgttt tgggacgct catgacccg cgattatta 
      atctgtgat gcgaaaaaa gtcaagtta ggcttgggt 
      gagaaaaaa ttgaaacgg tcgcggtct gataccaaa 
      accacgctc aaggatcag gaccatatt attataacg 
      atgtttgtg tcgtccgaa cacgggatt aaaatgcac 
      gatattagg ggtgtggag gctgagagt cggttactg 
      gccttagct acgccctat ttggtgatt ttggagaag 
      ttggtcata gttcgggac gttatgagt gattttaac 
      gggcttgaa agttgtaat aaggaaacg cgcgatgct 
      gttttgaat tttagttat aatttgagt cttggtaac 
      atggatgag gcctttaag gcgattaaa ttggtgcaa 
      aatccgggc gtttggggt agtttagcg aggatgtgt 
      gtcaagacg aggcgtttg gatgtggct ggggtttgt 
      ctggggcac atgggcaat gcacgggct gcgatggca 
      ctccgagtg gctgtggct gattattcg ttgccacat 
      gaggccaag ttggccgtt ttggccgta catttgggg 
      atgcttgac gaggccgag caattgtac atccaatgc 
      cagcgttac gaccttttg aataaattg ctccgaagt 
      cggaataaa ttcgacgct gctcatgcc attgcagaa 
      tcgcaagat cgcatccat ttgaaaaac actgaccac 
      gcatggggt aaagccctt gaagccgtg ggggatttt 
      aaagaggca gctgttcgt tacgaaaag gccaacact 
      cataaattc gatgttcca agaatgctt ttagatcaa 
      ccgcaacaa cttgaaact tatatggga aaaactaaa 
      gaccctgat ttgcttaag tggtggggt cagtatgtt 
      gagtctcaa ggggagatg caagccgcc ttgaaaatt 
      tatagtagt gcaggtgat gtttattcg caagtgagg 
      gtgctttgt tttcttggg gaggaaagt aaagcggct 
      gaattggca aggtcggga cgggataag gccgcgttt 
      tatcacatg gctaggttt tacgaaacg acagggaat 
      tttgaagag gctgtgaat tttttcaca aaggctaat 
      gcctacagt aacgcggtc cgactttgc aaagagaac 
      cacatgagc gacgaatta tggaacatc ggctctgtt 
      gccggaact cgcgaaaaa ctggaatgt gcgcgctat 
      ttcgaggaa attggggcc cttgacaag tcggttatt 
      ttataccac agggccgga atgctgcac aaggccctc 
      gaccttgca tttaaagcc caacaatac gacattctc 
      caacaaatc gccacagac ttggacgct gatagtgac 
      ccagctttg gtgcaaaaa tgcgccgat tatttcgta 
      acaaacgaa cagttcgat aaagctgtt gatttactc 
      gcaattgcg aaaaaatac aaagaggcg atcaccatt 
      tgtctgaca cataacgtg caactgact gaagattta 
      gccgaaaag ttaacacct gagaaagac caactggag 
      gaagcggac cgtgttaat gtcttgcaa acgttggct 
      gaaagttta atgttgcaa ggcaattac cacttggcc 
      acgaaaaaa ttcacacaa gccggcgat aagattcgg 
      gcaatgaaa gcattgcta aaaagtggc gataccgac 
      aaaatcatt ttttttgcg ggcgtttcg cgccaaaga 
      gagatttac ataatggca gcgaattat ttacaatcg 
      ctggattgg caaaataat cccgagatt ttacgcaac 
      attattacg ttttattcg aaaggcaaa gctttggat 
      cttttggcc aatttttac gtggcttgt gcacaagtc 
      gagattgac gaattcaaa aattacgaa aaggcgttt 
      ggggccttg actgaggcg tcgcggtgt ttggctaaa 
      ataggcaat cccagggac tcaagccaa catcagaga 
      gcgaccgaa atcgtccaa caaagactc acaatgatt 
      aagcggttt gttgatatt aggagatta tttgatcgg 
      ggggattac caagccggc attgcacaa tgtcgccag 
      ttgttaatg accggaggc aaggaattg gaagaatcg 
      gttcgcagg ggcgatatt tgtgcccta atgatacag 
      gagtacatc aaaagtggg aattttacc gaagctaaa 
      caattacta ggggaattg aaacagtta ttgttcagt 
      agtgggaac acaccgatt acttactac ctgaataag 
      gaaacaatt gaagcactg gcccatggt ttaggtgtc 
      cctgtttca accctaatt cccgctaaa atgacactc 
      gaggatgaa gacaccgag gaatttgtt gaagaagca 
      attgaagat tag
    mRNA sequence
    • >TC006692 RA
      cgccatgta aacaaggaa aaaatgaca ctttatttt 
      gaaaatccg gtgagtttt ccggagccg ggctcgatt 
      tccctgaac ggggtctgg cacccgaac tcggccctt 
      ctggccgtg gccagtttt tcgcaagat aagggcggt 
      tacgtgacg attttcgac gagttgggg gagcctttg 
      agcgatata aactacccc gtacaccgc agttttcaa 
      gtaacggct cttgcctgg caccccgag cgccatgtc 
      ctggccacc gggtgggaa aacggcgag tttaaaatc 
      tggaacggg gagaaggat tttgcgctc attgggggc 
      ccgcacaag gcccccatc acgctgctg gagttcagc 
      gaaaagggg ggccggctt gtgtcgtgc gattcggcg 
      gggtcgctt gtggggtgg aaggttgtg gatagtcag 
      gcgcagact gttatggtt tttcacttg gatttgaag 
      gagtcgatt acgcatttg acgttcagg atcacgata 
      aagccgcat ccggatttt gatgttgag gggttggct 
      aaggcggcg gttaatggg gatgaaagg gccctcgat 
      ctttttagt aattggcgt ccgaagacc acagcccgg 
      aaattccgt gttcaggag ggttctgac aacttgtca 
      ttttacgtg gggacgcaa gtgggctca atttactac 
      gttaatagc gggggctcg tgcatggaa gtcttaaac 
      acggaaggg gtgcccctt tcttacatt gtgcaccat 
      ccgagcaaa gacgcccta attgtcatg atggagggt 
      ttaacaatc ggtcatttc tccgttgat agtcaaggc 
      cagttaaac gaattagct aaagttaaa ttaagcgga 
      agggtgcaa agtcgcggt gttggaaca cagggctta 
      gtctggtgt tccaactcc agtttggcg gttttaacc 
      ggtgattta atcgtccgc atttgggac attgaaacc 
      aacgataat tacgtgtta cccacgtcg ttgaaattc 
      tacacaaac gacgataaa aatcgcaca gttagtgaa 
      gttttcacg tgcttggcg ttctgcaaa ttgaaccaa 
      actctgtgt gcgggcacc aacattggt cgcatttat 
      ttctggacc aggaacaca aacaatggg gaaagtccc 
      gaggattgt tgggagttg aataacgta aatacggtc 
      agcggaacc gttaaacaa ttaatgtgg ggttctgtt 
      cagttaaga ctcccgtta ttgagcgtt aattgcgtc 
      acgtgtgtt tacataatg aaggaacaa tcgatttgt 
      acgtgtttt tccgatgag gtttggtgc acgcaaaaa 
      accgccagt caggtgctt gtcgaaagc gccaaaagc 
      aacgttttg ctgactttg gagacacaa atcactgat 
      atgtgtatc aatgattcg tttgcaatt ttcacaaac 
      gggagacgc gttctagtt tatgaaatt ttctgggat 
      gggtctgac tcggaaaag ttcgaactc aatacgaac 
      atttccaag cagaccgaa agttcggcc gatttttcg 
      attaaacta atcagcacg tttgcgtgt gacaatgag 
      aaggtttta ctccacaaa aaaatgatc gttgttttg 
      accgcgaaa gcggtcaca ctccgacaa ttaaacggt 
      tcggttata accaccatc gtgtcaccc tcacacgag 
      ggcgaaccg atcggactc gacgtcacg gggaattac 
      ctcacgatt ttcacaatc gaaggctac atgaaagtg 
      tacgaactg ggcgagaat gaggcgaaa ctgtcgatt 
      ccggcccgg aatttgtac gacatgtgc ccggacttt 
      ggcgaaata atccaggcg aaaagcaac tcaagcggc 
      aataaaatc gccctgact ttagccgcg gccaatcta 
      gtgcccgat gggcgcctg tacgtctgg gacgtggaa 
      aatgacaat ttgctatcg tacgatttc cacaagtac 
      ggtaatttt gatttggag ccaagtccg gacgaattc 
      agcgtcgac gaacgaagc gaacaaaat gattcgtca 
      aacgacgac gaaaccgtt gcggttttc gacgaaatt 
      tgctcgaat cgcatccct ttgcacttg ttttgggac 
      gctcatgac ccgcgatta ttaatctgt gatgcgaaa 
      aaagtcaag ttaggcttg ggtgagaaa aaattgaaa 
      cggtcgcgg tctgatacc aaaaccacg ctcaaggat 
      caggaccat attattata acgatgttt gtgtcgtcc 
      gaacacggg attaaaatg cacgatatt aggggtgtg 
      gaggctgag agtcggtta ctggcctta gctacgccc 
      tatttggtg attttggag aagttggtc atagttcgg 
      gacgttatg agtgatttt aacgggctt gaaagttgt 
      aataaggaa acgcgcgat gctgttttg aattttagt 
      tataatttg agtcttggt aacatggat gaggccttt 
      aaggcgatt aaattggtg caaaatccg ggcgtttgg 
      ggtagttta gcgaggatg tgtgtcaag acgaggcgt 
      ttggatgtg gctggggtt tgtctgggg cacatgggc 
      aatgcacgg gctgcgatg gcactccga gtggctgtg 
      gctgattat tcgttgcca catgaggcc aagttggcc 
      gttttggcc gtacatttg gggatgctt gacgaggcc 
      gagcaattg tacatccaa tgccagcgt tacgacctt 
      ttgaataaa ttgctccga agtcggaat aaattcgac 
      gctgctcat gccattgca gaatcgcaa gatcgcatc 
      catttgaaa aacactgac cacgcatgg ggtaaagcc 
      cttgaagcc gtgggggat tttaaagag gcagctgtt 
      cgttacgaa aaggccaac actcataaa ttcgatgtt 
      ccaagaatg cttttagat caaccgcaa caacttgaa 
      acttatatg ggaaaaact aaagaccct gatttgctt 
      aagtggtgg ggtcagtat gttgagtct caaggggag 
      atgcaagcc gccttgaaa atttatagt agtgcaggt 
      gatgtttat tcgcaagtg agggtgctt tgttttctt 
      ggggaggaa agtaaagcg gctgaattg gcaaggtcg 
      ggacgggat aaggccgcg ttttatcac atggctagg 
      ttttacgaa acgacaggg aattttgaa gaggctgtg 
      aattttttc acaaaggct aatgcctac agtaacgcg 
      gtccgactt tgcaaagag aaccacatg agcgacgaa 
      ttatggaac atcggctct gttgccgga actcgcgaa 
      aaactggaa tgtgcgcgc tatttcgag gaaattggg 
      gcccttgac aagtcggtt attttatac cacagggcc 
      ggaatgctg cacaaggcc ctcgacctt gcatttaaa 
      gcccaacaa tacgacatt ctccaacaa atcgccaca 
      gacttggac gctgatagt gacccagct ttggtgcaa 
      aaatgcgcc gattatttc gtaacaaac gaacagttc 
      gataaagct gttgattta ctcgcaatt gcgaaaaaa 
      tacaaagag gcgatcacc atttgtctg acacataac 
      gtgcaactg actgaagat ttagccgaa aagttaaca 
      cctgagaaa gaccaactg gaggaagcg gaccgtgtt 
      aatgtcttg caaacgttg gctgaaagt ttaatgttg 
      caaggcaat taccacttg gccacgaaa aaattcaca 
      caagccggc gataagatt cgggcaatg aaagcattg 
      ctaaaaagt ggcgatacc gacaaaatc atttttttt 
      gcgggcgtt tcgcgccaa agagagatt tacataatg 
      gcagcgaat tatttacaa tcgctggat tggcaaaat 
      aatcccgag attttacgc aacattatt acgttttat 
      tcgaaaggc aaagctttg gatcttttg gccaatttt 
      tacgtggct tgtgcacaa gtcgagatt gacgaattc 
      aaaaattac gaaaaggcg tttggggcc ttgactgag 
      gcgtcgcgg tgtttggct aaaataggc aatcccagg 
      gactcaagc caacatcag agagcgacc gaaatcgtc 
      caacaaaga ctcacaatg attaagcgg tttgttgat 
      attaggaga ttatttgat cggggggat taccaagcc 
      ggcattgca caatgtcgc cagttgtta atgaccgga 
      ggcaaggaa ttggaagaa tcggttcgc aggggcgat 
      atttgtgcc ctaatgata caggagtac atcaaaagt 
      gggaatttt accgaagct aaacaatta ctaggggaa 
      ttgaaacag ttattgttc agtagtggg aacacaccg 
      attacttac tacctgaat aaggaaaca attgaagca 
      ctggcccat ggtttaggt gtccctgtt tcaacccta 
      attcccgct aaaatgaca ctcgaggat gaagacacc 
      gaggaattt gttgaagaa gcaattgaa gattagttt 
      aagtttaaa aacgaatcc gtccatttg tgtgattaa 
      ataaaacca ttcaaattg t
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