Status
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Gene information
General information
TC030563 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15641773..15643160
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030563
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06368

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030563 RA
      MFKFGFSSD DPQEKTAVS TEQGLNWLE SYQIRPENV 
      TITGLSPNS CLCGEFDVK FVSNRDVLS VLEQGGTNS 
      LLSAEKNHS DLIPAVYEG GLKVWECTF DLVDFLVEQ 
      KIDFGGKDV LDLGCGAGI AGILACLKG ARTVFQDYN 
      IEVIESLTI PNVYLNGLD LANCAFFCG DWGSFLDLQ 
      LKSAARKYD FILTSETIY NTNNYKKIL SIFKQLLKP 
      TGMIFLAAK YHYFGVGGG IPQFEDLLK MEDVFTHTI 
      CWQNSSGVK RAIIKISFK N
    • >TC030563 RB
      MILRKKRLV PYLAGLSGV TSLFHVLVS TEQGLNWLE 
      SYQIRPENV TITGLSPNS CLCGEFDVK FVSNRDVLS 
      VLEQGGTNS LLSAEKNHS DLIPAVYEG GLKVWECTF 
      DLVDFLVEQ KIDFGGKDV LDLGCGAGI AGILACLKG 
      ARTVFQDYN IEVIESLTI PNVYLNGLD LANCAFFCG 
      DWGSFLDLQ LKSAARKYD FILTSETIY NTNNYKKIL 
      SIFKQLLKP TGMIFLAAK YHYFGVGGG IPQFEDLLK 
      MEDVFTHTI CWQNSSGVK RAIIKISFK N
    CDS sequence
    • >TC030563 RA
      atgtttaag tttggtttt tcgagcgat gatcctcag 
      gaaaaaacg gctgtttcg actgaacaa gggttaaat 
      tggctcgaa agttaccaa attcgcccc gaaaacgtg 
      actataact ggcttgagc ccgaattcg tgtctttgt 
      ggcgaattt gacgttaag tttgtatca aatagggat 
      gttttgagt gttttggaa caggggggc actaattcc 
      cttctaagc gctgaaaaa aaccattct gatttaatt 
      cctgctgtg tatgaaggg gggctgaag gtctgggaa 
      tgcaccttt gacttggtg gatttttta gtcgaacaa 
      aaaattgat tttgggggc aaggacgtt ctggatttg 
      ggctgtggg gctggtatt gcggggatt ttggcttgt 
      ttgaagggg gcacggaca gtttttcaa gattataat 
      attgaagtg atagaatcg ctgacgatt cccaacgtt 
      tatttaaac ggcttagat ttggccaat tgtgcgttt 
      ttttgtggc gattggggc tcatttctc gacctacaa 
      ctcaaaagt gcggctagg aagtacgat tttatttta 
      acatcagag acaatctac aacacaaac aattataaa 
      aaaatattg agcatcttt aaacagtta ttaaaacca 
      actggaatg atttttttg gcagccaag taccactac 
      tttggtgtg gggggcgga atcccccaa ttcgaagac 
      ttgttaaaa atggaagac gttttcacc cacacgata 
      tgttggcaa aactctagc ggtgtaaaa cgggcaata 
      ataaaaatt tctttcaaa aactaa
    • >TC030563 RB
      atgatcctc aggaaaaaa cggctggtg ccctattta 
      gcgggcttg tctggtgta acctcactt ttccacgtt 
      ttagtttcg actgaacaa gggttaaat tggctcgaa 
      agttaccaa attcgcccc gaaaacgtg actataact 
      ggcttgagc ccgaattcg tgtctttgt ggcgaattt 
      gacgttaag tttgtatca aatagggat gttttgagt 
      gttttggaa caggggggc actaattcc cttctaagc 
      gctgaaaaa aaccattct gatttaatt cctgctgtg 
      tatgaaggg gggctgaag gtctgggaa tgcaccttt 
      gacttggtg gatttttta gtcgaacaa aaaattgat 
      tttgggggc aaggacgtt ctggatttg ggctgtggg 
      gctggtatt gcggggatt ttggcttgt ttgaagggg 
      gcacggaca gtttttcaa gattataat attgaagtg 
      atagaatcg ctgacgatt cccaacgtt tatttaaac 
      ggcttagat ttggccaat tgtgcgttt ttttgtggc 
      gattggggc tcatttctc gacctacaa ctcaaaagt 
      gcggctagg aagtacgat tttatttta acatcagag 
      acaatctac aacacaaac aattataaa aaaatattg 
      agcatcttt aaacagtta ttaaaacca actggaatg 
      atttttttg gcagccaag taccactac tttggtgtg 
      gggggcgga atcccccaa ttcgaagac ttgttaaaa 
      atggaagac gttttcacc cacacgata tgttggcaa 
      aactctagc ggtgtaaaa cgggcaata ataaaaatt 
      tctttcaaa aactaa
    mRNA sequence
    • >TC030563 RA
      aaaaagcgc aaaccaata agttacaaa aaggggctt 
      tcgcccgac aaaacaatt tattgcggt ttcgaagcg 
      cttggactt cgtgcattc cagattgtg aggttggaa 
      acgcaataa tttaagacc caattttat tgaaaatac 
      gttattgtc gttgttggg gaccagtac gtaatgttt 
      aagtttggt ttttcgagc gatgatcct caggaaaaa 
      acggctgtt tcgactgaa caagggtta aattggctc 
      gaaagttac caaattcgc cccgaaaac gtgactata 
      actggcttg agcccgaat tcgtgtctt tgtggcgaa 
      tttgacgtt aagtttgta tcaaatagg gatgttttg 
      agtgttttg gaacagggg ggcactaat tcccttcta 
      agcgctgaa aaaaaccat tctgattta attcctgct 
      gtgtatgaa ggggggctg aaggtctgg gaatgcacc 
      tttgacttg gtggatttt ttagtcgaa caaaaaatt 
      gattttggg ggcaaggac gttctggat ttgggctgt 
      ggggctggt attgcgggg attttggct tgtttgaag 
      ggggcacgg acagttttt caagattat aatattgaa 
      gtgatagaa tcgctgacg attcccaac gtttattta 
      aacggctta gatttggcc aattgtgcg tttttttgt 
      ggcgattgg ggctcattt ctcgaccta caactcaaa 
      agtgcggct aggaagtac gattttatt ttaacatca 
      gagacaatc tacaacaca aacaattat aaaaaaata 
      ttgagcatc tttaaacag ttattaaaa ccaactgga 
      atgattttt ttggcagcc aagtaccac tactttggt 
      gtggggggc ggaatcccc caattcgaa gacttgtta 
      aaaatggaa gacgttttc acccacacg atatgttgg 
      caaaactct agcggtgta aaacgggca ataataaaa 
      atttctttc aaaaactaa ggtgctagt ctttcataa 
      acccatccc tcggcccct tcatgggtc aaaacccca 
      tcagcttgc acccccttc ttgaacctg atccggtcg 
      tggatacgg tccgtctga ccttgccag aattcgatg 
      gtatggggg cgcacgatg tacccgccc cattgtggg 
      ggccgtggc acttgcccc tcgccgta
    • >TC030563 RB
      aaaaagcgc aaaccaata agttacaaa aaggggctt 
      tcgcccgac aaaacaatt tattgcggt ttcgaagcg 
      cttggactt cgtgcattc cagattgtg aggttggaa 
      acgcaataa tttaagacc caattttat tgaaaatac 
      gttattgtc gttgttggg gaccagtac gtaatgttt 
      aagtttggt ttttcgagc gatgatcct caggaaaaa 
      acggctggt gccctattt agcgggctt gtctggtgt 
      aacctcact tttccacgt tttagtttc gactgaaca 
      agggttaaa ttggctcga aagttacca aattcgccc 
      cgaaaacgt gactataac tggcttgag cccgaattc 
      gtgtctttg tggcgaatt tgacgttaa gtttgtatc 
      aaataggga tgttttgag tgttttgga acagggggg 
      cactaattc ccttctaag cgctgaaaa aaaccattc 
      tgatttaat tcctgctgt gtatgaagg ggggctgaa 
      ggtctggga atgcacctt tgacttggt ggatttttt 
      agtcgaaca aaaaattga ttttggggg caaggacgt 
      tctggattt gggctgtgg ggctggtat tgcggggat 
      tttggcttg tttgaaggg ggcacggac agtttttca 
      agattataa tattgaagt gatagaatc gctgacgat 
      tcccaacgt ttatttaaa cggcttaga tttggccaa 
      ttgtgcgtt tttttgtgg cgattgggg ctcatttct 
      cgacctaca actcaaaag tgcggctag gaagtacga 
      ttttatttt aacatcaga gacaatcta caacacaaa 
      caattataa aaaaatatt gagcatctt taaacagtt 
      attaaaacc aactggaat gattttttt ggcagccaa 
      gtaccacta ctttggtgt ggggggcgg aatccccca 
      attcgaaga cttgttaaa aatggaaga cgttttcac 
      ccacacgat atgttggca aaactctag cggtgtaaa 
      acgggcaat aataaaaat ttctttcaa aaactaagg 
      tgctagtct ttcataaac ccatccctc ggccccttc 
      atgggtcaa aaccccatc agcttgcac ccccttctt 
      gaacctgat ccggtcgtg gatacggtc cgtctgacc 
      ttgccagaa ttcgatggt atgggggcg cacgatgta 
      cccgcccca ttgtggggg ccgtggcac ttgcccctc 
      gccgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY