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Gene information
General information
TC030543 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2264088..2265965
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030543
    iBeetle screen iB_06367

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030543 RA
      MLNIRKVVV GISGGVDSA VAALLLKKR GYDIQGVFM 
      QNWDIADEK GYCSADADY KDAALVCDK LDIKLTHVN 
      FVKHYWNEV FSNLIKEYE TGNTPNPDV LCNRNIKFN 
      YFYKYARNE LGADAIATG HYARTSFGS YLEHYNPEK 
      GVRLLNAVD WFKDQTFFL CQVNQEALQ RTMFPLGDL 
      MKPEVKKIA RENGLEKIA RKKESMGIC FIGNRKFQD 
      FIREYIDDK PGDFVDVDT AKVVGQHNG VHQWTLGQR 
      ARLHSYESP YFVARKDVD KNIIYVVKG TKHPLLYTN 
      IILTSEPHW IHSQPPDLQ NGVLECDFK FQHTKYWVK 
      CQVCQTSKG LIIKVNRYK RAITPGQFA VLSKDGECL 
      GGARIINTG VSHFSLYYL ENNGLGSVS HILKKYVNV 
      TQGEDELTD NEDMKHRAV NRC
    CDS sequence
    • >TC030543 RA
      atgttaaac atccgaaag gtggtcgtg ggcatatct 
      gggggcgtc gatagcgcc gttgcggca cttttattg 
      aaaaaacgc ggttatgat atacagggt gttttcatg 
      caaaactgg gacattgcg gacgaaaag ggctactgt 
      tcggctgac gctgactac aaagacgct gctctagtg 
      tgtgacaaa ctggacatt aaactaact cacgtcaat 
      tttgtcaaa cattactgg aacgaagtg tttagcaat 
      ttaatcaaa gaatatgag accggcaac acgccaaac 
      cctgacgtt ctatgcaat cgtaacatt aaatttaat 
      tatttttac aaatacgcg agaaatgag ctaggggct 
      gacgctatc gcgaccgga cattacgca aggactagt 
      ttcgggtct tatcttgaa cattataac ccggaaaag 
      ggggtaaga ctactaaac gcagttgat tggttcaaa 
      gaccaaacg ttcttcttg tgccaagtc aatcaagaa 
      gctttgcaa cgtacaatg ttcccattg ggcgattta 
      atgaagccg gaagtgaaa aaaattgca agggaaaac 
      ggacttgaa aaaatcgca aggaagaag gagagtatg 
      gggatctgt tttatcggc aatcgcaaa tttcaagac 
      tttattaga gaatatatt gatgataaa ccgggcgat 
      ttcgtggac gttgacact gcgaaagtc gtgggtcaa 
      cacaatggg gtgcaccag tggactttg gggcaaagg 
      gcgcgcctc cacagctac gaaagccct tatttcgtg 
      gcccggaaa gacgtagac aagaacata atttacgta 
      gtcaaaggg acaaaacac ccgttactt tacacgaac 
      ataattttg accagtgag ccccactgg atccactca 
      cagccccct gatttacaa aacggggtt ttggagtgc 
      gatttcaag ttccaacac accaaatat tgggtcaaa 
      tgccaggtg tgccaaaca agtaaagga ctgattatt 
      aaagtgaat aggtacaag agggccatc actccggga 
      cagttcgct gtcttgtcg aaagatggc gagtgtctc 
      ggaggggct cgaataatt aacacggga gtttcacac 
      ttctcgcta tattatttg gagaataat ggtctaggg 
      agtgtctcc cacattttg aaaaaatat gtcaatgtg 
      acgcaaggt gaagacgag cttactgat aatgaagat 
      atgaagcac agagctgta aatagatgt tag
    mRNA sequence
    • >TC030543 RA
      caagattac aaaaatgaa tatcgtttc cctgttatt 
      ggacactct aaacaaatc ataatttaa ataattatt 
      aaaacgtga tctccgttt tggcgcatt ttttcgctt 
      gttaccgct gatcaaccc agttgtgct accgctgac 
      ttttgtcga attttgttg gttatgttc ctttaaacg 
      gcgaaaaaa gcatgttaa acatccgaa aggtggtcg 
      tgggcatat ctgggggcg tcgatagcg ccgttgcgg 
      cacttttat tgaaaaaac gcggttatg atatacagg 
      gtgttttca tgcaaaact gggacattg cggacgaaa 
      agggctact gttcggctg acgctgact acaaagacg 
      ctgctctag tgtgtgaca aactggaca ttaaactaa 
      ctcacgtca attttgtca aacattact ggaacgaag 
      tgtttagca atttaatca aagaatatg agaccggca 
      acacgccaa accctgacg ttctatgca atcgtaaca 
      ttaaattta attattttt acaaatacg cgagaaatg 
      agctagggg ctgacgcta tcgcgaccg gacattacg 
      caaggacta gtttcgggt cttatcttg aacattata 
      acccggaaa agggggtaa gactactaa acgcagttg 
      attggttca aagaccaaa cgttcttct tgtgccaag 
      tcaatcaag aagctttgc aacgtacaa tgttcccat 
      tgggcgatt taatgaagc cggaagtga aaaaaattg 
      caagggaaa acggacttg aaaaaatcg caaggaaga 
      aggagagta tggggatct gttttatcg gcaatcgca 
      aatttcaag actttatta gagaatata ttgatgata 
      aaccgggcg atttcgtgg acgttgaca ctgcgaaag 
      tcgtgggtc aacacaatg gggtgcacc agtggactt 
      tggggcaaa gggcgcgcc tccacagct acgaaagcc 
      cttatttcg tggcccgga aagacgtag acaagaaca 
      taatttacg tagtcaaag ggacaaaac acccgttac 
      tttacacga acataattt tgaccagtg agccccact 
      ggatccact cacagcccc ctgatttac aaaacgggg 
      ttttggagt gcgatttca agttccaac acaccaaat 
      attgggtca aatgccagg tgtgccaaa caagtaaag 
      gactgatta ttaaagtga ataggtaca agagggcca 
      tcactccgg gacagttcg ctgtcttgt cgaaagatg 
      gcgagtgtc tcggagggg ctcgaataa ttaacacgg 
      gagtttcac acttctcgc tatattatt tggagaata 
      atggtctag ggagtgtct cccacattt tgaaaaaat 
      atgtcaatg tgacgcaag gtgaagacg agcttactg 
      ataatgaag atatgaagc acagagctg taaatagat 
      gttagtaaa tcagcgctt tttcttatt cggactccg 
      cgaccgctt cctcctatc ctcgtcctc tgacacgct 
      gtcttcagc ggagtaatc cgaccc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY