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Gene information
General information
TC016383 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6785383..6787610
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016383
    iBeetle screen iB_06366

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Predominatly is the abdominal musculature affected.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen segment dorsal dorsalized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: All abdominal segments are present, but dorsal part of the segments are squeezed together.
    abdomen bristle potentially pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: Potentially secondary defect due to the squeezed segments.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016383 RA
      MSTKNVKRS LRTISAHGK LEAIRRVHD GESKASVAR 
      DIGVPESTL RGWCKNESK ISYLSRQSS PDTDESIEP 
      KDKRPKIED PIVQPFNLS LKSSNIYTP TNSESLYTP 
      MDYTKNDID CTPKTPQNL SIKTETTPK TASQITERE 
      RNRAELARL SVELGLNRP EMFTSSSST NNNTSSLAD 
      LAANINLLS QWNNILMQH QLNKKVTPD TTTPSSSTL 
      KQQNSIKPP KEQQSVEDY INSWLKAQE AMLSHSRSN 
      GLPPVTTTT NSTSNMQSS WFWNWYKQL SYQQSATPA 
      VSPEKPILY QQLTKDSSD AENLSVDKS NNNKVKSVL 
      DNLLHINNN NVTMDKKDC LSYSEAVEH GEKFLKWLE 
      SCSNPSVTA MQIMQFRAL LSNVKNSAD RKNGDLQNK 
      TKVKRK
    CDS sequence
    • >TC016383 RA
      atgtccacg aagaacgtc aagcgctcg ttgcgcact 
      attagcgcc cacggaaag ctggaggcc atcaggagg 
      gtgcatgac ggcgagagc aaggcctcg gtcgcgagg 
      gacatcggc gtgcccgag tccaccctc aggggatgg 
      tgcaagaac gaaagcaaa atttcctat ctctctcga 
      cagtccagc cccgacacg gacgaatcc atcgaaccc 
      aaagacaag cggccgaaa atcgaagat ccgatagta 
      cagccgttc aacctcagc ctaaaatcg tcaaacatt 
      tacacaccc accaattcc gagtctctg tacacgccg 
      atggactac actaaaaac gacatcgat tgcacgccg 
      aaaacgccg caaaatctc agcatcaag acggaaact 
      acccccaag acagcctcc caaataacc gaacgcgag 
      cgaaaccga gcggaattg gcaaggttg agcgtcgaa 
      cttggcttg aaccgaccc gaaatgttc acctcctcg 
      tcttcaacc aacaacaac acctctagt cttgccgat 
      ctagccgcg aacatcaac ctcctcagc cagtggaac 
      aacattctg atgcaacat caactcaac aagaaagtc 
      acccccgac acgacaact ccaagcagc tcaaccctc 
      aaacaacag aactcgatc aaaccgccc aaagaacaa 
      cagtcggtt gaagattac atcaacagt tggttgaaa 
      gcgcaagag gcgatgttg agtcacagc cgatcgaat 
      ggacttccc ccggtcacg acaacaaca aattcaaca 
      tcaaacatg caaagttcg tggttttgg aattggtat 
      aagcagctc tcgtatcag cagagtgcc acacctgcc 
      gtatccccg gaaaagccg attttgtat caacaattg 
      acaaaggat tcatccgat gcggaaaat ctctcggtt 
      gataagtcg aacaacaac aaagtgaag tcggtcttg 
      gacaatttg cttcatatc aacaacaat aacgtgacg 
      atggataaa aaggattgc ttgtcgtat agtgaggcc 
      gtcgagcac ggggagaaa ttcttgaag tggctggaa 
      tcttgcagt aacccttcg gtgacggca atgcagata 
      atgcagttc agggcgttg ttgagtaac gtgaagaac 
      agtgcggac aggaagaat ggtgacctg cagaacaaa 
      accaaagtt aaaagaaag tga
    mRNA sequence
    • >TC016383 RA
      ccgtgttcg tccgtcgcc agtattgat cgcctggac 
      aaaggaact gaccaccgg ctggccaac ctcgcaaga 
      aactactac ttgccacta gccggccgt cgcgagcgg 
      atgtccgca cacccatca tacccggtg tttgagaaa 
      agcgcgcgc ctgcgtcac ccccaactg cattcgtca 
      aaacttggc cgcaagttt cgtttgttt gtgtgaagt 
      ttttgccgg tgaggtttt ttacaactg cgcactcgg 
      gcgcactcc cattggccc gttacacgc ttccaccac 
      gtgctcctc tcggtggag acgatgtcc acgaagaac 
      gtcaagcgc tcgttgcgc actattagc gcccacgga 
      aagctggag gccatcagg agggtgcat gacggcgag 
      agcaaggcc tcggtcgcg agggacatc ggcgtgccc 
      gagtccacc ctcagggga tggtgcaag aacgaaagc 
      aaaatttcc tatctctct cgacagtcc agccccgac 
      acggacgaa tccatcgaa cccaaagac aagcggccg 
      aaaatcgaa gatccgata gtacagccg ttcaacctc 
      agcctaaaa tcgtcaaac atttacaca cccaccaat 
      tccgagtct ctgtacacg ccgatggac tacactaaa 
      aacgacatc gattgcacg ccgaaaacg ccgcaaaat 
      ctcagcatc aagacggaa actaccccc aagacagcc 
      tcccaaata accgaacgc gagcgaaac cgagcggaa 
      ttggcaagg ttgagcgtc gaacttggc ttgaaccga 
      cccgaaatg ttcacctcc tcgtcttca accaacaac 
      aacacctct agtcttgcc gatctagcc gcgaacatc 
      aacctcctc agccagtgg aacaacatt ctgatgcaa 
      catcaactc aacaagaaa gtcaccccc gacacgaca 
      actccaagc agctcaacc ctcaaacaa cagaactcg 
      atcaaaccg cccaaagaa caacagtcg gttgaagat 
      tacatcaac agttggttg aaagcgcaa gaggcgatg 
      ttgagtcac agccgatcg aatggactt cccccggtc 
      acgacaaca acaaattca acatcaaac atgcaaagt 
      tcgtggttt tggaattgg tataagcag ctctcgtat 
      cagcagagt gccacacct gccgtatcc ccggaaaag 
      ccgattttg tatcaacaa ttgacaaag gattcatcc 
      gatgcggaa aatctctcg gttgataag tcgaacaac 
      aacaaagtg aagtcggtc ttggacaat ttgcttcat 
      atcaacaac aataacgtg acgatggat aaaaaggat 
      tgcttgtcg tatagtgag gccgtcgag cacggggag 
      aaattcttg aagtggctg gaatcttgc agtaaccct 
      tcggtgacg gcaatgcag ataatgcag ttcagggcg 
      ttgttgagt aacgtgaag aacagtgcg gacaggaag 
      aatggtgac ctgcagaac aaaaccaaa gttaaaaga 
      aagtgaatt tactttcgt ctagtgaaa aaaggctgt 
      ttagctacc aaatcttat tagttaaaa tattatgta 
      gtttatgta aaaagtcta tacgggtgt tgattcgat 
      ggatacggc gttggtagt aaccattaa atttacgcc 
      cgttcttcc ttaaatgtt accttgaca aaccgttta 
      ttgacgctt aaaatttgt ttgtacata cgttacttt 
      agtcgaatt tagataaat ttaattgta aagactatt 
      aatagtgcc aaatgattt tcgcattat ttttgctca 
      aatatgtac ctaactgtg tgttataga ccagttttt 
      ttcctattt cagatctca tgtcattgt ctgccaaag 
      tttttttca ccgcgcggc caattggtc gacatttgt 
      ttgcaatac atgtcagaa tttattcct tagatttaa 
      gtgatttaa ctgttatac agggtggtg tgtactttc 
      agtgattta ttgccctgt atcgaattt cgctaaaat 
      tatggtgtt atttgtaca ttgttgtat aataaattt 
      atctaatat ttttgtacc aaattaaat aaaattctt 
      cgacattga gctgtcttt tcattctcc agaacccct 
      cgaaaacgt aaaaaataa atcatccga ggcgaaaca 
      gcttccagt aaattttaa aacaaatta ttacgtccc 
      gtttaactc gataataaa gtttgaacc ttgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY