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Gene information
General information
TC016222 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4404779..4406055
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016222
    iBeetle screen iB_06364

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna pedicellus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016222 RA
      MGVPWLSQV RFLYMVILL ITTALCLLY VAYITSFQL 
      TRTASPLGT LVLSEILSF EENTTQAEV AKNTTVANS 
      SSEAPTVTT TNSTPPDLT PRVAAEVLY EAGHVDFGP 
      QICPDFGRD LKLLIAITS APGHESARM AIRETWGHF 
      AIRNDVAVA FMLGLISNE TVNAKIEKE QDLYGDLIR 
      GKFTDTYDN LTLKTISLL EWVDNYCPE AAFLLKTDD 
      DMFINVSRL LDFIAKRNP EQRTIFGRL AKKWIPVRN 
      RKSKYYVSP NQFKPAVFP EFTTGPAYL LPVHLAKEL 
      YLAALNHTY CKLEDVFIT GVVAKNLKI KRVNVPEFL 
      NQRVSLTAC RVQEGISIH MVEGMEQYD VWKKLHEVD 
      PECEE
    CDS sequence
    • >TC016222 RA
      atgggggtg ccatggctc tcccaagtg cgcttcctg 
      tacatggtg attttactc ataacgaca gcgttatgt 
      ttactgtac gtggcatat ataacatcc ttccaattg 
      acaagaaca gcttcaccc ttaggcacg ttagtatta 
      tccgaaatt ctttcattc gaagaaaac acaacccaa 
      gctgaagtg gcgaaaaat acgacagtg gcgaactca 
      tcatctgaa gccccaact gtaacaaca acaaatagc 
      actccaccg gacctaact ccgcgagtt gccgctgaa 
      gtactgtac gaggcaggt catgtggac tttggtccg 
      caaatctgt cccgatttt ggaagagat ttaaagttg 
      ctgattgcc ataacgtcg gcaccaggc cacgaaagc 
      gcccgaatg gccattcgg gagacttgg ggccacttt 
      gctatcaga aatgacgta gctgtagct tttatgtta 
      ggtttgatt tccaatgaa acagttaac gcaaaaata 
      gagaaggaa caagacttg tacggtgat ttaatccga 
      gggaagttc acagacacg tacgacaat ctcactctt 
      aaaacgatt tcgttgtta gagtgggtt gataattac 
      tgtccagaa gcagctttt ttacttaaa accgacgac 
      gatatgttt attaatgtc tctcggctc ttggatttc 
      attgccaag cgcaatcca gagcaaagg acgatattc 
      ggccgcttg gcgaaaaag tggataccg gtcagaaac 
      agaaaatcc aaatactac gtttcgcca aatcagttc 
      aaaccggcg gtttttccc gaatttacc accggacct 
      gcatatcta ttgccagtt catttggcc aaagaactg 
      tacctagct gctcttaat cacacgtat tgtaagcta 
      gaggatgtg tttattacg ggagttgtg gcaaagaat 
      ttgaaaatc aaacgagta aatgttccg gaatttcta 
      aaccaaaga gtttctcta actgcatgt agagtccag 
      gaagggatt agcatacat atggtcgag ggtatggaa 
      cagtacgat gtgtggaaa aaattacac gaagttgac 
      ccagagtgc gaggaataa
    mRNA sequence
    • >TC016222 RA
      ggcaacatt aacggaact atcaaagac ggggacgtt 
      ttatttgta cgtcttata gttttgaca cctgtcact 
      caaaaaaat tggcatggg ggtgccatg gctctccca 
      agtgcgctt cctgtacat ggtgatttt actcataac 
      gacagcgtt atgtttact gtacgtggc atatataac 
      atccttcca attgacaag aacagcttc acccttagg 
      cacgttagt attatccga aattctttc attcgaaga 
      aaacacaac ccaagctga agtggcgaa aaatacgac 
      agtggcgaa ctcatcatc tgaagcccc aactgtaac 
      aacaacaaa tagcactcc accggacct aactccgcg 
      agttgccgc tgaagtact gtacgaggc aggtcatgt 
      ggactttgg tccgcaaat ctgtcccga ttttggaag 
      agatttaaa gttgctgat tgccataac gtcggcacc 
      aggccacga aagcgcccg aatggccat tcgggagac 
      ttggggcca ctttgctat cagaaatga cgtagctgt 
      agcttttat gttaggttt gatttccaa tgaaacagt 
      taacgcaaa aatagagaa ggaacaaga cttgtacgg 
      tgatttaat ccgagggaa gttcacaga cacgtacga 
      caatctcac tcttaaaac gatttcgtt gttagagtg 
      ggttgataa ttactgtcc agaagcagc ttttttact 
      taaaaccga cgacgatat gtttattaa tgtctctcg 
      gctcttgga tttcattgc caagcgcaa tccagagca 
      aaggacgat attcggccg cttggcgaa aaagtggat 
      accggtcag aaacagaaa atccaaata ctacgtttc 
      gccaaatca gttcaaacc ggcggtttt tcccgaatt 
      taccaccgg acctgcata tctattgcc agttcattt 
      ggccaaaga actgtacct agctgctct taatcacac 
      gtattgtaa gctagagga tgtgtttat tacgggagt 
      tgtggcaaa gaatttgaa aatcaaacg agtaaatgt 
      tccggaatt tctaaacca aagagtttc tctaactgc 
      atgtagagt ccaggaagg gattagcat acatatggt 
      cgagggtat ggaacagta cgatgtgtg gaaaaaatt 
      acacgaagt tgacccaga gtgcgagga ataaactat 
      tagaaaatt ttcaactgt acagtttca cagttaatt 
      aattatcaa tcaaaaaat taagaatta tgtataaat 
      aaacattaa gttgcata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY