Status
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Gene information
General information
TC016196 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4596660..4612657
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016196
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06362

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016196 RA
      MTSFDFTWN NAAQVTGKA FVRKKSSKK VSTQKQTAP 
      PKKAPESRT AKNKSLEPD DNQQEKLSG YVKRKQKKD 
      KLAAKAASD GNQQISKGS GDKSATTSY SLFAVRHKD 
      IYVNTNIKG KSVVEKVFS GEKKFSDLQ IHKYLVANL 
      QKHSFVNLT NVQERAIPE ILAGKNVLI RSQTGSGKT 
      LAYALPIMN ALLSVEPRL QRQDGVQAI IVVPTRELA 
      LQTHEIFGK INTFQWLVI GHLCGGENR KTEKDKLRK 
      GVHVVIGTP GRLLDHILH TSAFKTENV KCLVLDEAD 
      RLLDMGFKK DIVKIVEAL DRTKQHSDY DPMAMLLHK 
      KTQVKVESK NQEKKRQTI LLSATLNKG IAELADFLM 
      KNHTYIDTL DDSPDINPD HMVIPNTVT QEFIMTHIK 
      HRLFTLSAV LLAKAKHKV FVFMATSQM VDYHYDLFT 
      KYFAKMPKN RGKLKIGEV AVLENEDFE DSDEEEEVV 
      LESEFFKLH GNMDQGARK KVFMGFKAA KRGILLCTD 
      VAARGVDVP EADCIIQYT GPQSDDDYL HRVGRTGRA 
      GKSGSSLIF LTHEEQEYI ARLQDHKVF LKERQSSEF 
      LKHLCTLME EPEEEKAAT ALQRRFETA LTKDKDLHR 
      LACFAYSSW SRFYNTFPG QLRSIFNFK TVNLGHYVT 
      SFGLRETPS SVARIVKGQ VSTVQPRRL NKKLATHGD 
      EEEVKTHKR KIKSVSLTT SEFASGLEP KKKKKKKKT 
      QT
    CDS sequence
    • >TC016196 RA
      atgacttcg tttgatttt acttggaat aacgcagcg 
      caagttaca gggaaagca tttgtgcgt aaaaaatca 
      tctaaaaaa gtctccaca caaaaacaa acagccccc 
      cctaaaaaa gctccagag tcaagaact gcgaaaaat 
      aaatcactc gaacccgat gataatcaa caagagaaa 
      ttatcagga tacgttaaa aggaaacag aaaaaagat 
      aaattagct gcgaaagca gcaagtgat ggaaatcaa 
      caaattagt aaaggaagt ggtgataaa agtgcaacc 
      acgtcatac tcattattt gccgtcaga cataaggat 
      atttacgtg aatacaaat attaaggga aaatcagtg 
      gtggagaaa gtgttcagt ggggaaaaa aagttcagt 
      gatttacaa atacataaa tatttggtg gccaattta 
      caaaaacat tcgtttgtt aacttgacg aatgttcaa 
      gagcgggcc attccggag attttggcg ggaaaaaac 
      gtactgatt cgttcccaa accggttct ggcaaaacc 
      ttagcgtac gcattacca ataatgaac gctcttcta 
      agtgtcgaa cctcgttta caacgacag gatggagtc 
      caagctatt atagtcgta ccaacgcga gaattagcc 
      ttacaaacg catgaaatc ttcggtaaa ataaacacg 
      tttcaatgg ctcgtaatc ggccattta tgcggcggc 
      gaaaaccga aaaactgag aaagataag ttacgtaaa 
      ggagtgcat gttgttatc ggtactcca ggtcggtta 
      ctagaccac attttacac acttctgct ttcaaaact 
      gaaaatgtt aaatgtcta gtgcttgac gaagccgat 
      cgtctccta gacatgggg tttaagaaa gatatagta 
      aaaatagta gaagcgtta gatcggacg aaacaacac 
      tctgattat gaccccatg gcgatgctt ttgcacaaa 
      aaaacacaa gtcaaagtc gaatcaaaa aaccaagaa 
      aaaaaacga caaacaata cttttatca gccactctc 
      aacaaaggc atagccgaa ctagcagac tttttaatg 
      aaaaaccac acttatatc gacactctt gacgactct 
      cccgacata aaccccgac cacatggtc ataccaaac 
      accgtcacc caggagttt attatgact catattaaa 
      cacagactt ttcactttg tcggccgta cttttggcc 
      aaagccaag cacaaagtg ttcgttttc atggccacg 
      agccaaatg gttgattac cattacgat ttatttacg 
      aaatatttt gcaaaaatg cccaaaaat cggggaaaa 
      ttgaaaatt ggagaagtg gcggttttg gaaaatgag 
      gattttgag gacagtgac gaggaggag gaagttgtg 
      ctcgagagc gaatttttc aagctgcat ggaaacatg 
      gatcagggg gctaggaag aaggttttt atggggttc 
      aaggccgcc aaaagggga attctatta tgcacggat 
      gtagcagct cgtggtgtt gacgtccct gaagcagac 
      tgtataatt caatataca ggtccgcaa tcagacgat 
      gattatctt caccgagtt ggaagaacc ggaagggct 
      ggaaaatca ggctcttcc ttgatcttc ctcactcat 
      gaagaacaa gaatacatt gccagattg caggatcac 
      aaagtattt ttaaaagaa cggcaatct tccgaattt 
      ctcaaacac ctatgcact ttgatggaa gaacccgaa 
      gaagaaaaa gccgcaaca gctctgcaa cgtcgcttc 
      gaaacggcg ctgacgaag gacaaggac ttgcaccgt 
      cttgcctgc ttcgcatac tcctcctgg tcccgcttc 
      tacaacacg ttccccggc cagctgcgt tccattttc 
      aattttaaa accgtcaat ttaggtcac tatgtcaca 
      agtttcggg cttagggaa acccccagc agtgttgca 
      agaatcgtc aaaggtcaa gtatcaaca gtgcagcca 
      cgaagactc aacaaaaaa ctggccacc catggggat 
      gaagaagag gtgaagacg cacaagagg aagatcaag 
      tcggtcagt ttgaccacg tcggagttc gccagcgga 
      ttggaaccc aaaaagaag aagaagaag aagaaaact 
      caaacttga
    mRNA sequence
    • >TC016196 RA
      ctcctccaa agcaaatca atggccatc gactattac 
      aacttcgac attgacgta cacgtgtgt taacctaac 
      tttttatcg aagttaaaa gtgttataa ttggctaaa 
      aaacgtgtt tacgtttaa ataaaaaat gaaaaaggt 
      gcaaatgcc accaaaata atcaactaa aagtgttat 
      ttattgtct tttggatga tttaacccc ccttgtgtt 
      ttctctatt attcggatt tttttggtt aaaagtgca 
      attttaaca atgacttcg tttgatttt acttggaat 
      aacgcagcg caagttaca gggaaagca tttgtgcgt 
      aaaaaatca tctaaaaaa gtctccaca caaaaacaa 
      acagccccc cctaaaaaa gctccagag tcaagaact 
      gcgaaaaat aaatcactc gaacccgat gataatcaa 
      caagagaaa ttatcagga tacgttaaa aggaaacag 
      aaaaaagat aaattagct gcgaaagca gcaagtgat 
      ggaaatcaa caaattagt aaaggaagt ggtgataaa 
      agtgcaacc acgtcatac tcattattt gccgtcaga 
      cataaggat atttacgtg aatacaaat attaaggga 
      aaatcagtg gtggagaaa gtgttcagt ggggaaaaa 
      aagttcagt gatttacaa atacataaa tatttggtg 
      gccaattta caaaaacat tcgtttgtt aacttgacg 
      aatgttcaa gagcgggcc attccggag attttggcg 
      ggaaaaaac gtactgatt cgttcccaa accggttct 
      ggcaaaacc ttagcgtac gcattacca ataatgaac 
      gctcttcta agtgtcgaa cctcgttta caacgacag 
      gatggagtc caagctatt atagtcgta ccaacgcga 
      gaattagcc ttacaaacg catgaaatc ttcggtaaa 
      ataaacacg tttcaatgg ctcgtaatc ggccattta 
      tgcggcggc gaaaaccga aaaactgag aaagataag 
      ttacgtaaa ggagtgcat gttgttatc ggtactcca 
      ggtcggtta ctagaccac attttacac acttctgct 
      ttcaaaact gaaaatgtt aaatgtcta gtgcttgac 
      gaagccgat cgtctccta gacatgggg tttaagaaa 
      gatatagta aaaatagta gaagcgtta gatcggacg 
      aaacaacac tctgattat gaccccatg gcgatgctt 
      ttgcacaaa aaaacacaa gtcaaagtc gaatcaaaa 
      aaccaagaa aaaaaacga caaacaata cttttatca 
      gccactctc aacaaaggc atagccgaa ctagcagac 
      tttttaatg aaaaaccac acttatatc gacactctt 
      gacgactct cccgacata aaccccgac cacatggtc 
      ataccaaac accgtcacc caggagttt attatgact 
      catattaaa cacagactt ttcactttg tcggccgta 
      cttttggcc aaagccaag cacaaagtg ttcgttttc 
      atggccacg agccaaatg gttgattac cattacgat 
      ttatttacg aaatatttt gcaaaaatg cccaaaaat 
      cggggaaaa ttgaaaatt ggagaagtg gcggttttg 
      gaaaatgag gattttgag gacagtgac gaggaggag 
      gaagttgtg ctcgagagc gaatttttc aagctgcat 
      ggaaacatg gatcagggg gctaggaag aaggttttt 
      atggggttc aaggccgcc aaaagggga attctatta 
      tgcacggat gtagcagct cgtggtgtt gacgtccct 
      gaagcagac tgtataatt caatataca ggtccgcaa 
      tcagacgat gattatctt caccgagtt ggaagaacc 
      ggaagggct ggaaaatca ggctcttcc ttgatcttc 
      ctcactcat gaagaacaa gaatacatt gccagattg 
      caggatcac aaagtattt ttaaaagaa cggcaatct 
      tccgaattt ctcaaacac ctatgcact ttgatggaa 
      gaacccgaa gaagaaaaa gccgcaaca gctctgcaa 
      cgtcgcttc gaaacggcg ctgacgaag gacaaggac 
      ttgcaccgt cttgcctgc ttcgcatac tcctcctgg 
      tcccgcttc tacaacacg ttccccggc cagctgcgt 
      tccattttc aattttaaa accgtcaat ttaggtcac 
      tatgtcaca agtttcggg cttagggaa acccccagc 
      agtgttgca agaatcgtc aaaggtcaa gtatcaaca 
      gtgcagcca cgaagactc aacaaaaaa ctggccacc 
      catggggat gaagaagag gtgaagacg cacaagagg 
      aagatcaag tcggtcagt ttgaccacg tcggagttc 
      gccagcgga ttggaaccc aaaaagaag aagaagaag 
      aagaaaact caaacttga atgtaattt ttaaccaat 
      ttttggaaa attatattt atcttaact atttgcgtt 
      ttttaaccc tcttcccat taagtagga aaaaaataa 
      tatgcaact cgggaggta agtattatt ttatcttga 
      aattttatc aaataaaac tacaccgat tgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY