Status
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Gene information
General information
TC015766 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7757162..7763443
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015766
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06357

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015766 RA
      MVRGRRVKA VFACTRPDD CCSPHPKRR GPKITLETN 
      TITDFFKGQ DGRDDIVLL QVDDIDDVP KTPVIKNMT 
      CKEGPPTPH RIELCPDEA KIETKPKAK KRLNCQLST 
      DPKDNNHKV TDYFPVRRS VRKTKKTVM EEKQRTLEE 
      LLRNGVEEG LEVRIFSDK GRGVVTSRP FTKGDFVVE 
      YSGDLIDIN EAKRRELKY AQDLNTGCY MYYFKHNNQ 
      QYCVDATAE SGKLGRLVN HSRNGNLIT RTVAVDNKP 
      RLVLIAKDD IKAGDEITY DYGDRSKES LKHHPWLAY
    CDS sequence
    • >TC015766 RA
      atggtcagg ggtcgtcgc gtgaaggcc gttttcgca 
      tgcacccgt cctgacgat tgctgctct ccacacccg 
      aaacgtcgc ggtcccaaa atcacactc gagactaac 
      acaatcacc gactttttc aaggggcag gacgggcgt 
      gatgacatc gtgctgttg caagttgat gacattgat 
      gatgttcct aagacgccg gtgatcaaa aatatgacg 
      tgcaaggaa ggaccaccg actccacat cgaatcgaa 
      ctgtgccct gacgaagcc aaaattgag acgaaaccc 
      aaagccaaa aaacggttg aactgtcag ctgtcaacc 
      gaccccaag gacaacaac cacaaggtc accgactac 
      tttccggtg cgccgttcg gttcgcaaa acgaaaaag 
      acggtcatg gaagaaaaa caaagaact ttagaggag 
      ttgttacga aacggcgtt gaagaaggt ctcgaggtc 
      aggattttc tcggacaaa ggtcgcggt gtggtcact 
      tcgcgccct ttcaccaaa ggcgatttc gtagttgaa 
      tacagtggc gacctcatc gacattaac gaagcgaaa 
      agacgcgaa ttgaaatac gcccaagat ttaaatact 
      gggtgttac atgtattat tttaaacat aacaatcaa 
      cagtattgt gtggatgcg accgccgaa agtggtaag 
      ttggggaga ttggtgaac cattcgcgg aacggtaat 
      ctcatcacc aggaccgta gcggtggat aataagccc 
      cggcttgta cttattgca aaggatgat atcaaggcc 
      ggcgatgag attacttac gattacggt gacagatcg 
      aaagaatcg ctaaagcat catccgtgg ttagcctat 
      taa
    mRNA sequence
    • >TC015766 RA
      accgaccaa tcgcaacaa agtcgaatt ttaaagttg 
      cgacgcttg gcttgtggt ggttcaagc cgatttatt 
      atcgaattt taacccaaa gaaagtaga aaaatgtag 
      aaaaaaagt gccccaacg cttaatatt attgacccg 
      cgtcgcgtt ttattgttt tgagttcgg ttttgttca 
      agttgcggg cgataaatt ggtttcttg agggcgcgt 
      tttgggaca cattattgt gtaaaatgg tcaggggtc 
      gtcgcgtga aggccgttt tcgcatgca cccgtcctg 
      acgattgct gctctccac acccgaaac gtcgcggtc 
      ccaaaatca cactcgaga ctaacacaa tcaccgact 
      ttttcaagg ggcaggacg ggcgtgatg acatcgtgc 
      tgttgcaag ttgatgaca ttgatgatg ttcctaaga 
      cgccggtga tcaaaaata tgacgtgca aggaaggac 
      caccgactc cacatcgaa tcgaactgt gccctgacg 
      aagccaaaa ttgagacga aacccaaag ccaaaaaac 
      ggttgaact gtcagctgt caaccgacc ccaaggaca 
      acaaccaca aggtcaccg actactttc cggtgcgcc 
      gttcggttc gcaaaacga aaaagacgg tcatggaag 
      aaaaacaaa gaactttag aggagttgt tacgaaacg 
      gcgttgaag aaggtctcg aggtcagga ttttctcgg 
      acaaaggtc gcggtgtgg tcacttcgc gccctttca 
      ccaaaggcg atttcgtag ttgaataca gtggcgacc 
      tcatcgaca ttaacgaag cgaaaagac gcgaattga 
      aatacgccc aagatttaa atactgggt gttacatgt 
      attatttta aacataaca atcaacagt attgtgtgg 
      atgcgaccg ccgaaagtg gtaagttgg ggagattgg 
      tgaaccatt cgcggaacg gtaatctca tcaccagga 
      ccgtagcgg tggataata agccccggc ttgtactta 
      ttgcaaagg atgatatca aggccggcg atgagatta 
      cttacgatt acggtgaca gatcgaaag aatcgctaa 
      agcatcatc cgtggttag cctattaat ttatttgtt 
      tttttattt attattttt tcctttttt tgtaattgt 
      cggaaacgt gtagaaagg cacagtttg ttttaaagt 
      ggtcactgc cttttttat cttattttt ttgtgaatg 
      tacttggat agaagcaac ggttgacta aagattaga 
      taataaaag atttgtttt gtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY