Status
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Gene information
General information
TC015753 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7436873..7446075
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015753
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06355

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    All larvae on slide hatched, show no specific defects.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015753 RA
      MGVPKPQQR FYTPSSHPF NNNYMFNGD IASHSITGF 
YKNAKIFIT GATGFVDKA LVEKLLRTC DELDSLYLL 
MRPKRGMNI EQRLKELLK NPVFNRIRE KNPDAFDKV 
KAVAGDVSA PNLGLSDGD KEKLVEHCN IVFHSAATV 
KFNEDLKNA VTLNTLGTK QVLELCREM KNLKSFVHV 
STAYSNSDK YTVDETVYE PPHDPNAVI NSIKVLPNE 
GIEILSKKI LGKHPNTYT LTKAMAEHL VLESSSQIP 
AAVVRPSII TAAWKEPYP GWVDNVSGI TGIFMECGR 
GTIKSIICD DKCTMDLIP VDIVVNTII TAAWHTAAH 
PSNTMRVYN CTSGTTNPI SWKEFGRLT QKYWLEYPS 
KYVTWYPGF TYRTSRAMH VICAHLFHL LPSAFLDVF 
LYCTKQKPI MLKISRKFY SALDAGKFF STNEWDFKT 
STMAALVSA VRDAEDGAN FEIDMTAEN GFNWNFYLK 
DFTLGVRQY VLKDDISSL PSAKVKLNR LYWFQKVFQ 
AVTIYSIVK LALHQCT
    CDS sequence
    • >TC015753 RA
      atgggcgtc ccaaaaccg cagcaacgt ttctacacc 
ccctcgtcc caccccttc aacaacaac tacatgttc 
aacggtgac atcgcctcc cacagcatc accggattc 
tacaaaaac gcgaaaatc ttcatcacc ggagccacc 
ggattcgtg gacaaagcc ctcgtcgag aaactcctc 
cgaacatgc gacgaactc gacagtctt tatctttta 
atgcgacca aaacgcgga atgaacatc gaacagaga 
ttgaaggaa ttgctgaaa aacccagtt tttaaccgg 
attcgtgaa aaaaatccg gacgctttt gacaaagtc 
aaggccgtg gcgggggat gtttcagcc cccaatttg 
gggctgtca gatggggac aaggagaag ctggtcgag 
cactgcaat atcgttttc cattcagcg gccacagtc 
aaattcaac gaggatttg aaaaacgcc gtcaccctc 
aacaccttg ggcaccaaa caagtgctc gaactttgt 
cgagagatg aagaatttg aaaagtttc gtccacgtc 
tccacagct tacagcaat tcagacaag tacacggtt 
gacgaaacg gtgtatgaa cccccacat gaccccaac 
gcagtcatt aatagcatc aaagttttg ccaaacgaa 
ggaatcgaa atcttatca aaaaaaatc ttgggcaaa 
cacccaaac acctacacc ttgaccaag gcaatggcc 
gagcatttg gtccttgag agctcttcc cagatacca 
gcagctgtg gtacgccct tccatcatt acagccgca 
tggaaggaa ccataccca ggatgggtg gataacgtc 
agtgggatc acggggatt ttcatggaa tgtggtcga 
gggaccatc aagtcgatc atttgcgac gacaaatgt 
accatggac ttgatcccg gtcgatatc gtggtcaat 
accatcatc acggcggct tggcacacg gccgcacac 
ccatcgaac accatgcgg gtctacaac tgtaccagc 
ggtaccacc aaccccatt tcatggaag gagttcggc 
cgcttgacc cagaagtac tggctagag taccccagc 
aagtacgtc acgtggtac ccgggattc acctatcgc 
acaagccgc gccatgcac gtgatctgc gcccacttg 
ttccatttg ctgccctcg gccttcctt gatgtgttt 
ttgtattgc acgaaacaa aagccaata atgttgaaa 
atcagccga aagttctac agtgcgtta gatgcgggt 
aaatttttt tcaaccaac gaatgggat tttaagact 
tcgacgatg gcagcactg gtcagcgcc gtccgcgat 
gcagaagat ggcgccaat tttgaaatt gatatgaca 
gcggagaac ggcttcaat tggaacttc taccttaag 
gattttacc ctaggagtg agacagtac gtgctcaag 
gacgacatt agcagtcta ccaagtgcc aaagtcaaa 
ctaaacagg ttgtattgg ttccaaaag gtgttccaa 
gctgtcacg atatactct atagtcaaa ttggcactt 
catcaatgc acttag
    mRNA sequence
    • >TC015753 RA
      gtgcggcag tttccgcaa aagtggggc acccagacc 
agcatcagt tcattgtgg gcttgtaga cgattcggt 
tcaaaaaaa tgacagttt gaggcttta agtgcaaaa 
ttcaagtgg accaaaatc agggacaac acaaaaaat 
gggcgtccc aaaaccgca gcaacgttt ctacacccc 
ctcgtccca ccccttcaa caacaacta catgttcaa 
cggtgacat cgcctccca cagcatcac cggattcta 
caaaaacgc gaaaatctt catcaccgg agccaccgg 
attcgtgga caaagccct cgtcgagaa actcctccg 
aacatgcga cgaactcga cagtcttta tcttttaat 
gcgaccaaa acgcggaat gaacatcga acagagatt 
gaaggaatt gctgaaaaa cccagtttt taaccggat 
tcgtgaaaa aaatccgga cgcttttga caaagtcaa 
ggccgtggc gggggatgt ttcagcccc caatttggg 
gctgtcaga tggggacaa ggagaagct ggtcgagca 
ctgcaatat cgttttcca ttcagcggc cacagtcaa 
attcaacga ggatttgaa aaacgccgt caccctcaa 
caccttggg caccaaaca agtgctcga actttgtcg 
agagatgaa gaatttgaa aagtttcgt ccacgtctc 
cacagctta cagcaattc agacaagta cacggttga 
cgaaacggt gtatgaacc cccacatga ccccaacgc 
agtcattaa tagcatcaa agttttgcc aaacgaagg 
aatcgaaat cttatcaaa aaaaatctt gggcaaaca 
cccaaacac ctacacctt gaccaaggc aatggccga 
gcatttggt ccttgagag ctcttccca gataccagc 
agctgtggt acgcccttc catcattac agccgcatg 
gaaggaacc atacccagg atgggtgga taacgtcag 
tgggatcac ggggatttt catggaatg tggtcgagg 
gaccatcaa gtcgatcat ttgcgacga caaatgtac 
catggactt gatcccggt cgatatcgt ggtcaatac 
catcatcac ggcggcttg gcacacggc cgcacaccc 
atcgaacac catgcgggt ctacaactg taccagcgg 
taccaccaa ccccatttc atggaagga gttcggccg 
cttgaccca gaagtactg gctagagta ccccagcaa 
gtacgtcac gtggtaccc gggattcac ctatcgcac 
aagccgcgc catgcacgt gatctgcgc ccacttgtt 
ccatttgct gccctcggc cttccttga tgtgttttt 
gtattgcac gaaacaaaa gccaataat gttgaaaat 
cagccgaaa gttctacag tgcgttaga tgcgggtaa 
atttttttc aaccaacga atgggattt taagacttc 
gacgatggc agcactggt cagcgccgt ccgcgatgc 
agaagatgg cgccaattt tgaaattga tatgacagc 
ggagaacgg cttcaattg gaacttcta ccttaagga 
ttttaccct aggagtgag acagtacgt gctcaagga 
cgacattag cagtctacc aagtgccaa agtcaaact 
aaacaggtt gtattggtt ccaaaaggt gttccaagc 
tgtcacgat atactctat agtcaaatt ggcacttca 
tcaatgcac ttagcccaa tttttccat ttttgactc 
gttatagga gaattaagt gtcttatcg agaaatcga 
gtatcacca aacggcgga caaaccatg caaatgaca 
aattttagg ataggttat tgaatatct tactttatt 
tattctagt tttatagca ctaacatgt atttttcaa 
ctggaaaag gtttatgtt gataataaa atttgtatc 
actgg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY