Status
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Gene information
General information
TC015753 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7436873..7446075
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015753
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06355

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    All larvae on slide hatched, show no specific defects.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015753 RA
      MGVPKPQQR FYTPSSHPF NNNYMFNGD IASHSITGF 
      YKNAKIFIT GATGFVDKA LVEKLLRTC DELDSLYLL 
      MRPKRGMNI EQRLKELLK NPVFNRIRE KNPDAFDKV 
      KAVAGDVSA PNLGLSDGD KEKLVEHCN IVFHSAATV 
      KFNEDLKNA VTLNTLGTK QVLELCREM KNLKSFVHV 
      STAYSNSDK YTVDETVYE PPHDPNAVI NSIKVLPNE 
      GIEILSKKI LGKHPNTYT LTKAMAEHL VLESSSQIP 
      AAVVRPSII TAAWKEPYP GWVDNVSGI TGIFMECGR 
      GTIKSIICD DKCTMDLIP VDIVVNTII TAAWHTAAH 
      PSNTMRVYN CTSGTTNPI SWKEFGRLT QKYWLEYPS 
      KYVTWYPGF TYRTSRAMH VICAHLFHL LPSAFLDVF 
      LYCTKQKPI MLKISRKFY SALDAGKFF STNEWDFKT 
      STMAALVSA VRDAEDGAN FEIDMTAEN GFNWNFYLK 
      DFTLGVRQY VLKDDISSL PSAKVKLNR LYWFQKVFQ 
      AVTIYSIVK LALHQCT
    CDS sequence
    • >TC015753 RA
      atgggcgtc ccaaaaccg cagcaacgt ttctacacc 
      ccctcgtcc caccccttc aacaacaac tacatgttc 
      aacggtgac atcgcctcc cacagcatc accggattc 
      tacaaaaac gcgaaaatc ttcatcacc ggagccacc 
      ggattcgtg gacaaagcc ctcgtcgag aaactcctc 
      cgaacatgc gacgaactc gacagtctt tatctttta 
      atgcgacca aaacgcgga atgaacatc gaacagaga 
      ttgaaggaa ttgctgaaa aacccagtt tttaaccgg 
      attcgtgaa aaaaatccg gacgctttt gacaaagtc 
      aaggccgtg gcgggggat gtttcagcc cccaatttg 
      gggctgtca gatggggac aaggagaag ctggtcgag 
      cactgcaat atcgttttc cattcagcg gccacagtc 
      aaattcaac gaggatttg aaaaacgcc gtcaccctc 
      aacaccttg ggcaccaaa caagtgctc gaactttgt 
      cgagagatg aagaatttg aaaagtttc gtccacgtc 
      tccacagct tacagcaat tcagacaag tacacggtt 
      gacgaaacg gtgtatgaa cccccacat gaccccaac 
      gcagtcatt aatagcatc aaagttttg ccaaacgaa 
      ggaatcgaa atcttatca aaaaaaatc ttgggcaaa 
      cacccaaac acctacacc ttgaccaag gcaatggcc 
      gagcatttg gtccttgag agctcttcc cagatacca 
      gcagctgtg gtacgccct tccatcatt acagccgca 
      tggaaggaa ccataccca ggatgggtg gataacgtc 
      agtgggatc acggggatt ttcatggaa tgtggtcga 
      gggaccatc aagtcgatc atttgcgac gacaaatgt 
      accatggac ttgatcccg gtcgatatc gtggtcaat 
      accatcatc acggcggct tggcacacg gccgcacac 
      ccatcgaac accatgcgg gtctacaac tgtaccagc 
      ggtaccacc aaccccatt tcatggaag gagttcggc 
      cgcttgacc cagaagtac tggctagag taccccagc 
      aagtacgtc acgtggtac ccgggattc acctatcgc 
      acaagccgc gccatgcac gtgatctgc gcccacttg 
      ttccatttg ctgccctcg gccttcctt gatgtgttt 
      ttgtattgc acgaaacaa aagccaata atgttgaaa 
      atcagccga aagttctac agtgcgtta gatgcgggt 
      aaatttttt tcaaccaac gaatgggat tttaagact 
      tcgacgatg gcagcactg gtcagcgcc gtccgcgat 
      gcagaagat ggcgccaat tttgaaatt gatatgaca 
      gcggagaac ggcttcaat tggaacttc taccttaag 
      gattttacc ctaggagtg agacagtac gtgctcaag 
      gacgacatt agcagtcta ccaagtgcc aaagtcaaa 
      ctaaacagg ttgtattgg ttccaaaag gtgttccaa 
      gctgtcacg atatactct atagtcaaa ttggcactt 
      catcaatgc acttag
    mRNA sequence
    • >TC015753 RA
      gtgcggcag tttccgcaa aagtggggc acccagacc 
      agcatcagt tcattgtgg gcttgtaga cgattcggt 
      tcaaaaaaa tgacagttt gaggcttta agtgcaaaa 
      ttcaagtgg accaaaatc agggacaac acaaaaaat 
      gggcgtccc aaaaccgca gcaacgttt ctacacccc 
      ctcgtccca ccccttcaa caacaacta catgttcaa 
      cggtgacat cgcctccca cagcatcac cggattcta 
      caaaaacgc gaaaatctt catcaccgg agccaccgg 
      attcgtgga caaagccct cgtcgagaa actcctccg 
      aacatgcga cgaactcga cagtcttta tcttttaat 
      gcgaccaaa acgcggaat gaacatcga acagagatt 
      gaaggaatt gctgaaaaa cccagtttt taaccggat 
      tcgtgaaaa aaatccgga cgcttttga caaagtcaa 
      ggccgtggc gggggatgt ttcagcccc caatttggg 
      gctgtcaga tggggacaa ggagaagct ggtcgagca 
      ctgcaatat cgttttcca ttcagcggc cacagtcaa 
      attcaacga ggatttgaa aaacgccgt caccctcaa 
      caccttggg caccaaaca agtgctcga actttgtcg 
      agagatgaa gaatttgaa aagtttcgt ccacgtctc 
      cacagctta cagcaattc agacaagta cacggttga 
      cgaaacggt gtatgaacc cccacatga ccccaacgc 
      agtcattaa tagcatcaa agttttgcc aaacgaagg 
      aatcgaaat cttatcaaa aaaaatctt gggcaaaca 
      cccaaacac ctacacctt gaccaaggc aatggccga 
      gcatttggt ccttgagag ctcttccca gataccagc 
      agctgtggt acgcccttc catcattac agccgcatg 
      gaaggaacc atacccagg atgggtgga taacgtcag 
      tgggatcac ggggatttt catggaatg tggtcgagg 
      gaccatcaa gtcgatcat ttgcgacga caaatgtac 
      catggactt gatcccggt cgatatcgt ggtcaatac 
      catcatcac ggcggcttg gcacacggc cgcacaccc 
      atcgaacac catgcgggt ctacaactg taccagcgg 
      taccaccaa ccccatttc atggaagga gttcggccg 
      cttgaccca gaagtactg gctagagta ccccagcaa 
      gtacgtcac gtggtaccc gggattcac ctatcgcac 
      aagccgcgc catgcacgt gatctgcgc ccacttgtt 
      ccatttgct gccctcggc cttccttga tgtgttttt 
      gtattgcac gaaacaaaa gccaataat gttgaaaat 
      cagccgaaa gttctacag tgcgttaga tgcgggtaa 
      atttttttc aaccaacga atgggattt taagacttc 
      gacgatggc agcactggt cagcgccgt ccgcgatgc 
      agaagatgg cgccaattt tgaaattga tatgacagc 
      ggagaacgg cttcaattg gaacttcta ccttaagga 
      ttttaccct aggagtgag acagtacgt gctcaagga 
      cgacattag cagtctacc aagtgccaa agtcaaact 
      aaacaggtt gtattggtt ccaaaaggt gttccaagc 
      tgtcacgat atactctat agtcaaatt ggcacttca 
      tcaatgcac ttagcccaa tttttccat ttttgactc 
      gttatagga gaattaagt gtcttatcg agaaatcga 
      gtatcacca aacggcgga caaaccatg caaatgaca 
      aattttagg ataggttat tgaatatct tactttatt 
      tattctagt tttatagca ctaacatgt atttttcaa 
      ctggaaaag gtttatgtt gataataaa atttgtatc 
      actgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY