Status
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Gene information
General information
TC015749 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7406406..7412742
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015749
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06354

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015749 RA
      MSQFSTNEA VSLEKQVET VLNPATKFE LIDEVYELQ 
      RCAGWIKAN DFQKVCLQF PDHLLQDSS EVALRLGKQ 
      VGRTVYILA DTAYESCCI DYVAAGHIN ADAIIHFGP 
      VCFSKTSAN IPYLNIYEK DELDLGALE QCLVPLNEK 
      IVILLDTSF IHLTDKIVA KFPDHTIKT INSDNLTIE 
      NDTVVFIGG RNHHRKLPN LKFSHKPKT LYYFNDKAP 
      SLINYTLDE KVLRRRYFL VEKIKDSNT VGIVIGTLG 
      VSNYLKAIE RLKKLLTLH SKKYYLVSV GKPTVAKLA 
      NFPEIDIYV VVTCALNEI YDSRDYYKP IVTIYDVEM 
      ALNPAPEGL EFTYDYNDV ITRPLNDVT SDPNDVSLL 
      TGRIRGTST IQGAELVSK EAGTVALNT NHGAGFLAN 
      RTWKGLERD VGGDEVKMA SEGRRGIAQ SYNDETSG
    CDS sequence
    • >TC015749 RA
      atgtcgcag ttttccaca aacgaagcg gtcagtctc 
      gaaaaacaa gtagaaact gtgttaaat ccggcgacc 
      aaattcgaa ctaattgac gaggtatac gaactacag 
      cggtgtgcc ggatggatc aaagccaac gattttcaa 
      aaggtttgt ttgcaattc cccgaccat ctcttgcaa 
      gactcgtcg gaagtcgct ttgaggtta ggaaaacaa 
      gtcggacgg actgtctac attctagcg gacacggcg 
      tatgaaagc tgttgcatc gattacgtg gccgcgggt 
      catattaac gcagatgct attatccac tttggacct 
      gtttgtttc tcaaagacg tcggcaaat atcccctat 
      ttgaacatt tacgagaag gacgaactt gatttgggg 
      gcacttgag caatgtttg gtgccactg aacgagaaa 
      attgttatt ttattggac acttccttt attcatttg 
      acagacaaa attgtggcc aaattcccc gaccacacg 
      atcaaaact ataaattcc gacaatttg acgatagaa 
      aatgacaca gttgtgttt atcgggggc cgtaatcac 
      catcgcaag ttacctaac ctcaaattt tcgcataaa 
      ccgaaaacg ctgtattat ttcaatgac aaagccccg 
      agtttgatt aattacact ctcgacgaa aaagtctta 
      agacgaagg tattttcta gttgaaaaa attaaagat 
      agcaatact gttggtata gttatagga acgttgggg 
      gttagtaac tatttaaaa gccatagaa cggctcaaa 
      aaactccta actttgcac agtaagaaa tactattta 
      gtcagtgtt ggcaaaccg acagtcgca aaacttgcg 
      aattttcca gagattgac atttacgtg gtcgtaacg 
      tgcgccttg aacgaaatt tacgacagt cgtgactac 
      tacaagccc attgttacg atttacgac gtggaaatg 
      gcccttaat ccggccccc gaaggcctt gaattcact 
      tacgactac aatgacgtc atcacaagg ccactcaac 
      gacgtcacg agtgaccct aacgatgtc agtctactt 
      acggggcgt atccggggc acgtccact atccaaggg 
      gcggagctt gtgtcaaaa gaggcgggg actgtggct 
      ctcaacacc aatcatggc gccggtttc ctggccaat 
      aggacgtgg aaggggttg gagcgtgac gtgggtggg 
      gatgaagtc aagatggcg tcggagggg aggcgcggg 
      atcgcgcaa agctacaat gatgaaact agtggatga
    mRNA sequence
    • >TC015749 RA
      caggatgtt gtattttcg ttttttttt tgtctaact 
      atggctaca atatccaat tacaaataa aaataatta 
      ctcaccggt ccggcggtc cctgaacat attcaaaaa 
      catgcttag gcaaccagt tatacagcc atcaaggtt 
      tttcttacc ccttcactt tggctgcga aaaaagtgg 
      aaaaattac aattgcaaa atcaaaaat taccacgga 
      aatgtcatt atttacgta aaaaacatt tttatatta 
      acaataaca ctaaaactc ttctccaaa aattcaagt 
      aacaaaaaa tttttccaa cttacctat ccctggaat 
      aacaaaaga aaaacgcca aaaattgaa cagcgctca 
      acaaccgat cacagccga ctaagcaca atccactta 
      ttgtttccc agccgtctg tgattagtc gtataatta 
      ttctaataa caatgcaat tactgtacg ttttagaga 
      gcggcacga aagtcgaaa ttgggcgtt tttcccacc 
      acggacaat acaaacgct tgatttccg gtaattaaa 
      aatgtgcca aaaattttg gaaaaatag ttcaaatgc 
      cgtagttcg aatcattgg tcggtcaaa cgtcttgac 
      attgatttt gaaagggag caccgtcgt gtgcgaagt 
      cacgtaggc acatgttgg tggaaatcg gcgaaaaat 
      gtcgcagtt ttccacaaa cgaagcggt cagtctcga 
      aaaacaagt agaaactgt gttaaatcc ggcgaccaa 
      attcgaact aattgacga ggtatacga actacagcg 
      gtgtgccgg atggatcaa agccaacga ttttcaaaa 
      ggtttgttt gcaattccc cgaccatct cttgcaaga 
      ctcgtcgga agtcgcttt gaggttagg aaaacaagt 
      cggacggac tgtctacat tctagcgga cacggcgta 
      tgaaagctg ttgcatcga ttacgtggc cgcgggtca 
      tattaacgc agatgctat tatccactt tggacctgt 
      ttgtttctc aaagacgtc ggcaaatat cccctattt 
      gaacattta cgagaagga cgaacttga tttgggggc 
      acttgagca atgtttggt gccactgaa cgagaaaat 
      tgttatttt attggacac ttcctttat tcatttgac 
      agacaaaat tgtggccaa attccccga ccacacgat 
      caaaactat aaattccga caatttgac gatagaaaa 
      tgacacagt tgtgtttat cgggggccg taatcacca 
      tcgcaagtt acctaacct caaattttc gcataaacc 
      gaaaacgct gtattattt caatgacaa agccccgag 
      tttgattaa ttacactct cgacgaaaa agtcttaag 
      acgaaggta ttttctagt tgaaaaaat taaagatag 
      caatactgt tggtatagt tataggaac gttgggggt 
      tagtaacta tttaaaagc catagaacg gctcaaaaa 
      actcctaac tttgcacag taagaaata ctatttagt 
      cagtgttgg caaaccgac agtcgcaaa acttgcgaa 
      ttttccaga gattgacat ttacgtggt cgtaacgtg 
      cgccttgaa cgaaattta cgacagtcg tgactacta 
      caagcccat tgttacgat ttacgacgt ggaaatggc 
      ccttaatcc ggcccccga aggccttga attcactta 
      cgactacaa tgacgtcat cacaaggcc actcaacga 
      cgtcacgag tgaccctaa cgatgtcag tctacttac 
      ggggcgtat ccggggcac gtccactat ccaaggggc 
      ggagcttgt gtcaaaaga ggcggggac tgtggctct 
      caacaccaa tcatggcgc cggtttcct ggccaatag 
      gacgtggaa ggggttgga gcgtgacgt gggtgggga 
      tgaagtcaa gatggcgtc ggaggggag gcgcgggat 
      cgcgcaaag ctacaatga tgaaactag tggatgagt 
      tatttattt tgtggaaaa ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY