Status
Please wait ...
Gene information
General information
TC015600 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3320373..3323857
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015600
    iBeetle screen iB_06350

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015600 RA
      MELYKCQIY HTKGKEAFD CSNGGLEPY KCHKCNAFA 
TPLLSQLRY HILQGHTSQ CVEPTTYKC PSCDFKSFS 
EFLLSHHLN LTQHQSKDI YKCINCDYK TKFFVSLRK 
HFLQKHPDI VWFKCEHCP FKGINKNDL RRHLRQNHD 
PSLRYQCQY CDYKTKKNY TLRRHVITQ HELKQKHLP 
RTLICPKCQ FTTPFRHKL NKHVVNKHG PSRFKCEQC 
EFGTRYKYN LQKHVAVKH TNNPKVLKC QMCEYKPKH 
KDYLIDHIR TQHGVVPAF ECAPCEFKT NVKRSFVRH 
CRAHNESQK CNECGLSLQ NKLELAKHK KLEHLKVSV 
DVKVHKCQL CDFQSEYPG NVHRHKYNK HLDQWVYCA 
HCDHKTKRK DSLLEHLRT QHKLALI
    CDS sequence
    • >TC015600 RA
      atggaactg tacaagtgc caaatatac cataccaag 
ggcaaagaa gcatttgat tgctcaaac ggtggcctt 
gaaccctac aagtgtcac aaatgcaat gccttcgct 
acgccatta ttatcacaa ctgaggtat cacatcctc 
caaggtcac acatcacaa tgtgttgaa cccacgaca 
tacaaatgt ccctcatgt gatttcaag tcattttcg 
gaatttttg ctttcccat catttaaat ttaacacaa 
caccaaagc aaggacatc tacaaatgt ataaattgt 
gactacaag actaaattt ttcgtcagt ttgagaaaa 
cattttctc caaaaacat ccagacatt gtctggttc 
aaatgcgag cactgtccc tttaaggga ataaacaag 
aacgatttg cgccgccac cttagacaa aaccacgat 
cctagcctt cgatatcag tgccaatat tgcgattac 
aaaacgaaa aaaaactac actttgaga cgccatgtt 
atcactcaa cacgaactt aagcaaaaa catttaccc 
cggacgttg atttgcccc aaatgccag ttcaccacg 
cctttcaga cacaagttg aacaagcac gtggtgaac 
aaacacggc cctagtagg ttcaagtgc gagcaatgc 
gagtttggg accagatac aagtacaac ttgcaaaaa 
cacgtcgct gttaagcac acaaataac cccaaagtg 
ctcaaatgc cagatgtgc gaatataag cccaagcat 
aaggactac ctcatagac cacatacgg acccaacat 
ggcgtcgtt ccggcattc gagtgtgcg ccttgcgag 
ttcaaaaca aatgttaag cggtctttc gttagacat 
tgtcgggca cataatgaa tcccagaag tgtaacgag 
tgcggttta agtttacaa aataagttg gaacttgcc 
aaacacaaa aaacttgaa catttgaag gttagcgtg 
gatgttaaa gtgcacaaa tgccaactt tgtgatttt 
caaagtgaa tatccgggg aatgtccat aggcataag 
tacaataaa catttggat cagtgggtt tactgtgcg 
cattgtgat cacaaaacg aaaaggaag gacagtttg 
cttgagcat ctgaggaca cagcataag cttgccctt 
atctag
    mRNA sequence
    • >TC015600 RA
      attgtgttg aaaaacagc tattaaata cattgttat 
tctccgaaa tggaactgt acaagtgcc aaatatacc 
ataccaagg gcaaagaag catttgatt gctcaaacg 
gtggccttg aaccctaca agtgtcaca aatgcaatg 
ccttcgcta cgccattat tatcacaac tgaggtatc 
acatcctcc aaggtcaca catcacaat gtgttgaac 
ccacgacat acaaatgtc cctcatgtg atttcaagt 
cattttcgg aatttttgc tttcccatc atttaaatt 
taacacaac accaaagca aggacatct acaaatgta 
taaattgtg actacaaga ctaaatttt tcgtcagtt 
tgagaaaac attttctcc aaaaacatc cagacattg 
tctggttca aatgcgagc actgtccct ttaagggaa 
taaacaaga acgatttgc gccgccacc ttagacaaa 
accacgatc ctagccttc gatatcagt gccaatatt 
gcgattaca aaacgaaaa aaaactaca ctttgagac 
gccatgtta tcactcaac acgaactta agcaaaaac 
atttacccc ggacgttga tttgcccca aatgccagt 
tcaccacgc ctttcagac acaagttga acaagcacg 
tggtgaaca aacacggcc ctagtaggt tcaagtgcg 
agcaatgcg agtttggga ccagataca agtacaact 
tgcaaaaac acgtcgctg ttaagcaca caaataacc 
ccaaagtgc tcaaatgcc agatgtgcg aatataagc 
ccaagcata aggactacc tcatagacc acatacgga 
cccaacatg gcgtcgttc cggcattcg agtgtgcgc 
cttgcgagt tcaaaacaa atgttaagc ggtctttcg 
ttagacatt gtcgggcac ataatgaat cccagaagt 
gtaacgagt gcggtttaa gtttacaaa ataagttgg 
aacttgcca aacacaaaa aacttgaac atttgaagg 
ttagcgtgg atgttaaag tgcacaaat gccaacttt 
gtgattttc aaagtgaat atccgggga atgtccata 
ggcataagt acaataaac atttggatc agtgggttt 
actgtgcgc attgtgatc acaaaacga aaaggaagg 
acagtttgc ttgagcatc tgaggacac agcataagc 
ttgccctta tctagtggt ttgtgattt tggaacaca 
aaaaaattg aaggcggaa ttcaatata tatattttg 
gtttttgtg tggtagtta tgttggatt tggggacgt 
agggaatga agaaatgaa aagcttaaa tataggtta 
tttacttta attgcaaaa gtggttgta aaatgaaca 
agatacaat aattcttat tacgatgga aaactttta 
gcattatta atataaaaa tgattttcg tgtactttt 
cctgatgtt aaatgattt attattttt ttaatataa 
ccgatatca gattttcta gaagaaagt agcattagt 
tataacaat accatctat ctctaaaaa ataggatag 
ctgtttgta tccaaggga ggaaataca tgtttccaa 
ttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY