Status
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Gene information
General information
TC015600 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3320373..3323857
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015600
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06350

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015600 RA
      MELYKCQIY HTKGKEAFD CSNGGLEPY KCHKCNAFA 
      TPLLSQLRY HILQGHTSQ CVEPTTYKC PSCDFKSFS 
      EFLLSHHLN LTQHQSKDI YKCINCDYK TKFFVSLRK 
      HFLQKHPDI VWFKCEHCP FKGINKNDL RRHLRQNHD 
      PSLRYQCQY CDYKTKKNY TLRRHVITQ HELKQKHLP 
      RTLICPKCQ FTTPFRHKL NKHVVNKHG PSRFKCEQC 
      EFGTRYKYN LQKHVAVKH TNNPKVLKC QMCEYKPKH 
      KDYLIDHIR TQHGVVPAF ECAPCEFKT NVKRSFVRH 
      CRAHNESQK CNECGLSLQ NKLELAKHK KLEHLKVSV 
      DVKVHKCQL CDFQSEYPG NVHRHKYNK HLDQWVYCA 
      HCDHKTKRK DSLLEHLRT QHKLALI
    CDS sequence
    • >TC015600 RA
      atggaactg tacaagtgc caaatatac cataccaag 
      ggcaaagaa gcatttgat tgctcaaac ggtggcctt 
      gaaccctac aagtgtcac aaatgcaat gccttcgct 
      acgccatta ttatcacaa ctgaggtat cacatcctc 
      caaggtcac acatcacaa tgtgttgaa cccacgaca 
      tacaaatgt ccctcatgt gatttcaag tcattttcg 
      gaatttttg ctttcccat catttaaat ttaacacaa 
      caccaaagc aaggacatc tacaaatgt ataaattgt 
      gactacaag actaaattt ttcgtcagt ttgagaaaa 
      cattttctc caaaaacat ccagacatt gtctggttc 
      aaatgcgag cactgtccc tttaaggga ataaacaag 
      aacgatttg cgccgccac cttagacaa aaccacgat 
      cctagcctt cgatatcag tgccaatat tgcgattac 
      aaaacgaaa aaaaactac actttgaga cgccatgtt 
      atcactcaa cacgaactt aagcaaaaa catttaccc 
      cggacgttg atttgcccc aaatgccag ttcaccacg 
      cctttcaga cacaagttg aacaagcac gtggtgaac 
      aaacacggc cctagtagg ttcaagtgc gagcaatgc 
      gagtttggg accagatac aagtacaac ttgcaaaaa 
      cacgtcgct gttaagcac acaaataac cccaaagtg 
      ctcaaatgc cagatgtgc gaatataag cccaagcat 
      aaggactac ctcatagac cacatacgg acccaacat 
      ggcgtcgtt ccggcattc gagtgtgcg ccttgcgag 
      ttcaaaaca aatgttaag cggtctttc gttagacat 
      tgtcgggca cataatgaa tcccagaag tgtaacgag 
      tgcggttta agtttacaa aataagttg gaacttgcc 
      aaacacaaa aaacttgaa catttgaag gttagcgtg 
      gatgttaaa gtgcacaaa tgccaactt tgtgatttt 
      caaagtgaa tatccgggg aatgtccat aggcataag 
      tacaataaa catttggat cagtgggtt tactgtgcg 
      cattgtgat cacaaaacg aaaaggaag gacagtttg 
      cttgagcat ctgaggaca cagcataag cttgccctt 
      atctag
    mRNA sequence
    • >TC015600 RA
      attgtgttg aaaaacagc tattaaata cattgttat 
      tctccgaaa tggaactgt acaagtgcc aaatatacc 
      ataccaagg gcaaagaag catttgatt gctcaaacg 
      gtggccttg aaccctaca agtgtcaca aatgcaatg 
      ccttcgcta cgccattat tatcacaac tgaggtatc 
      acatcctcc aaggtcaca catcacaat gtgttgaac 
      ccacgacat acaaatgtc cctcatgtg atttcaagt 
      cattttcgg aatttttgc tttcccatc atttaaatt 
      taacacaac accaaagca aggacatct acaaatgta 
      taaattgtg actacaaga ctaaatttt tcgtcagtt 
      tgagaaaac attttctcc aaaaacatc cagacattg 
      tctggttca aatgcgagc actgtccct ttaagggaa 
      taaacaaga acgatttgc gccgccacc ttagacaaa 
      accacgatc ctagccttc gatatcagt gccaatatt 
      gcgattaca aaacgaaaa aaaactaca ctttgagac 
      gccatgtta tcactcaac acgaactta agcaaaaac 
      atttacccc ggacgttga tttgcccca aatgccagt 
      tcaccacgc ctttcagac acaagttga acaagcacg 
      tggtgaaca aacacggcc ctagtaggt tcaagtgcg 
      agcaatgcg agtttggga ccagataca agtacaact 
      tgcaaaaac acgtcgctg ttaagcaca caaataacc 
      ccaaagtgc tcaaatgcc agatgtgcg aatataagc 
      ccaagcata aggactacc tcatagacc acatacgga 
      cccaacatg gcgtcgttc cggcattcg agtgtgcgc 
      cttgcgagt tcaaaacaa atgttaagc ggtctttcg 
      ttagacatt gtcgggcac ataatgaat cccagaagt 
      gtaacgagt gcggtttaa gtttacaaa ataagttgg 
      aacttgcca aacacaaaa aacttgaac atttgaagg 
      ttagcgtgg atgttaaag tgcacaaat gccaacttt 
      gtgattttc aaagtgaat atccgggga atgtccata 
      ggcataagt acaataaac atttggatc agtgggttt 
      actgtgcgc attgtgatc acaaaacga aaaggaagg 
      acagtttgc ttgagcatc tgaggacac agcataagc 
      ttgccctta tctagtggt ttgtgattt tggaacaca 
      aaaaaattg aaggcggaa ttcaatata tatattttg 
      gtttttgtg tggtagtta tgttggatt tggggacgt 
      agggaatga agaaatgaa aagcttaaa tataggtta 
      tttacttta attgcaaaa gtggttgta aaatgaaca 
      agatacaat aattcttat tacgatgga aaactttta 
      gcattatta atataaaaa tgattttcg tgtactttt 
      cctgatgtt aaatgattt attattttt ttaatataa 
      ccgatatca gattttcta gaagaaagt agcattagt 
      tataacaat accatctat ctctaaaaa ataggatag 
      ctgtttgta tccaaggga ggaaataca tgtttccaa 
      ttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY