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Gene information
General information
TC015406 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 457068..462107
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015406
      iBeetle screen iB_06345

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015406 RA
        MPQATLLLR EETSSSLST LVEKSQHRK PPLPSYDLA 
        LTPTHFSLT PLPRPETDE GFESDTLSL VSSDGSYET 
        KTTETDSAN GSSTSDSDT ETPEATRAQ HHNLVDCVC 
        YTDVKVPDV LIFIIKSET LVFKFDNLD ALTRFYSNF 
        TAVKAVANQ KAYSKSGAK FNLLERTDN NGVTHIEIT 
        PLRKVWSSA DDLLSPRRP ERRKKGRAP PPPPTTSDV 
        LRGEYVRVN VSTAKDRDF IVKSQHNEK KPPVKLALF 
        RPVKTDSLM KQRSRSETR CFTPMAYRY IDTAPAYSP 
        MGNRLFGLS SKLRDFGEP IKECRWNNM AELGYTKEG 
        NLKSVIKKE ESKKKNEKK VTFSAYTTV QVV
      CDS sequence
      • >TC015406 RA
        atgccccag gccactctg cttcttaga gaagaaacc 
        tcttcatct ctgtccact ttagtcgaa aaatcgcaa 
        caccgcaaa ccccccctc ccttcatac gacctcgcc 
        ctcaccccc acccacttc tcccttacg cccctcccc 
        cgtccggaa acagacgaa ggcttcgaa tcggacact 
        ctttcccta gtttcaagt gatggaagt tacgaaacg 
        aaaacgacc gaaacggac agcgctaac ggttcgtca 
        acgtccgac agcgacacg gaaacgccc gaagccaca 
        cgagcccaa caccacaat ctagtcgac tgtgtttgc 
        tacacggat gtcaaagtc cccgatgtt ttgattttt 
        atcattaag agtgaaact ctcgtcttc aagtttgac 
        aatttggac gctttgacg cgattttat tcgaatttt 
        accgccgtt aaggctgtg gccaatcag aaggcttat 
        agcaaaagt ggcgccaaa ttcaatttg ttggaacgg 
        acggacaac aacggcgtt acgcacatt gagatcacg 
        cccttgagg aaggtgtgg tcgtcggct gatgatttg 
        ttgtcgcct aggcgacca gagaggcgc aagaaaggg 
        cgggcgccg ccccctccc cccaccacg tccgatgtg 
        ctgagaggg gagtatgtt agggttaat gtaagcacg 
        gccaaagat agggatttt attgttaaa agtcaacac 
        aatgagaag aaaccgccg gttaagttg gcgctgttc 
        cgcccggtt aaaaccgat tccctgatg aagcaacgc 
        agtaggagc gaaacgagg tgtttcacg cctatggcc 
        tataggtac attgatacg gcccccgcc tactccccg 
        atggggaac aggctgttt gggttgtcg tcaaaattg 
        agggatttt ggggaaccg atcaaagag tgccggtgg 
        aacaatatg gcggagttg ggttacacg aaagaggga 
        aatttaaaa agtgtgatt aagaaggag gaaagtaag 
        aagaagaat gagaagaaa gttactttt agtgcctac 
        acaaccgtg caggttgtc tga
      mRNA sequence
      • >TC015406 RA
        gtggaggtg cacagccta cttgctcgt tcccccttt 
        ttagtagca acaggtgtt ttgatcgat ttggaacgc 
        tgtgctttc agtgcccgc ttcgatttt gtttcaact 
        tgtgtcgga gtgagtttt gctgtgaca ttatggatt 
        atatcttca attttggat aatttttca aataatgcc 
        atcccaaga tgccccagg ccactctgc ttcttagag 
        aagaaacct cttcatctc tgtccactt tagtcgaaa 
        aatcgcaac accgcaaac cccccctcc cttcatacg 
        acctcgccc tcaccccca cccacttct cccttacgc 
        ccctccccc gtccggaaa cagacgaag gcttcgaat 
        cggacactc tttccctag tttcaagtg atggaagtt 
        acgaaacga aaacgaccg aaacggaca gcgctaacg 
        gttcgtcaa cgtccgaca gcgacacgg aaacgcccg 
        aagccacac gagcccaac accacaatc tagtcgact 
        gtgtttgct acacggatg tcaaagtcc ccgatgttt 
        tgattttta tcattaaga gtgaaactc tcgtcttca 
        agtttgaca atttggacg ctttgacgc gattttatt 
        cgaatttta ccgccgtta aggctgtgg ccaatcaga 
        aggcttata gcaaaagtg gcgccaaat tcaatttgt 
        tggaacgga cggacaaca acggcgtta cgcacattg 
        agatcacgc ccttgagga aggtgtggt cgtcggctg 
        atgatttgt tgtcgccta ggcgaccag agaggcgca 
        agaaagggc gggcgccgc cccctcccc ccaccacgt 
        ccgatgtgc tgagagggg agtatgtta gggttaatg 
        taagcacgg ccaaagata gggatttta ttgttaaaa 
        gtcaacaca atgagaaga aaccgccgg ttaagttgg 
        cgctgttcc gcccggtta aaaccgatt ccctgatga 
        agcaacgca gtaggagcg aaacgaggt gtttcacgc 
        ctatggcct ataggtaca ttgatacgg cccccgcct 
        actccccga tggggaaca ggctgtttg ggttgtcgt 
        caaaattga gggattttg gggaaccga tcaaagagt 
        gccggtgga acaatatgg cggagttgg gttacacga 
        aagagggaa atttaaaaa gtgtgatta agaaggagg 
        aaagtaaga agaagaatg agaagaaag ttactttta 
        gtgcctaca caaccgtgc aggttgtct gaaagaagg 
        gattttgta ctgatttat tattgtact tgtatattt 
        ctagcatag tttgtatat attctgact taatgtcaa 
        taaatatgt atataatct
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY