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Gene information
General information
TC015197 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1646600..1648927
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015197
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06343

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015197 RA
      MPLSPNLLQ RLAELKKWQ ESHEDLLKK TIINTSANA 
      TTLSDYQTI DGLSAFDSS NEHSRKNWE SQVISPTKT 
      FHELLEEKL ALDPGPGAA PPKPKRPFL KKGAGLERF 
      RMKTRPKLI KKSKGEKEN DNVTPLRAP DLVVRPKAT 
      WGPPGEQTL YEKALEKEL RIFEALEEK AENSSFCST 
      NSSVVRILS STPSKKGGD DVIETELER KDIAEILLR 
      LKNLAEGRM KPPDVPSII EASDEDKWT SSEVSSVSS 
      GDLEETLTA PKRDIGVGT EASNDCAIC GDKLKKKII 
      EYDDKLNDL IEDKNRISE LRKQLEQKE RDFLKRKHE 
      FEDQRANFE YEADIERKK LAKERQAFQ MFMKESQNR 
      PNKKERQEI SNLKQEVAD LSETLKLKE SKNGMTQAR 
      LRNQIKQLE KENSQLKTE IEKLTKENA KLSATQKLN 
      RKSSDSKIL HEINKNITK LTNQAKNHE KNVMLDQSE 
      SFLVMAPPL ADECNNGGA NQSETSAFN SNQSQVTYP 
      NGNIKTVSP DGNFITVKF FNGDRQETN LLDGTVKYF 
      FTNRNITQT TFADGLEVV EFPEGVIER RFPDGRCEI 
      TLPDGAVQT TLPDGGTET KYTDGSIVK IAPNGDKVL 
      LLANGQKEI ETKEYKSRE YPDGTVKIL YRDGTQETK 
      YANGRVRIK DSNGKLVLD THL
    CDS sequence
    • >TC015197 RA
      atgccactt tcaccaaac ctcctccaa cgcttggcc 
      gaactcaaa aaatggcaa gaaagccac gaggattta 
      ctcaaaaaa acaattatc aacaccagt gcaaacgcg 
      accacatta tcggactac caaaccatt gacggtctt 
      tcagccttt gactcctca aacgaacac tcccgtaaa 
      aactgggag agtcaggta atttccccc acgaaaacc 
      ttccacgaa ctacttgaa gaaaaactc gctcttgat 
      ccgggccct ggggctgcg ccccctaaa ccgaaacgc 
      cccttttta aaaaagggg gccggtttg gagcgtttc 
      cgcatgaaa acgcgcccc aaattgatt aaaaaatcg 
      aaaggggag aaagaaaat gataatgtg actcctctg 
      agggccccc gatttggtg gtgaggccc aaggccacg 
      tgggggccc cccggcgag caaacactg tacgagaag 
      gcgctagag aaggagttg aggattttt gaggctttg 
      gaggaaaag gccgagaat agtagcttt tgttcaacg 
      aattctagc gttgtgagg attttgtca agcactccg 
      agtaaaaaa gggggcgat gatgtgata gagactgag 
      ttggagcgc aaagacatt gcggagatt ttattgagg 
      cttaagaat ttggccgag ggacggatg aagcccccc 
      gatgtaccc tcgattatt gaggcttct gatgaggac 
      aagtggacc agtagtgag gtttcaagt gtctcatcg 
      ggggattta gaggagact ctgacagcc ccaaaacgt 
      gatatagga gttgggact gaggcaagc aatgattgt 
      gcaatttgt ggtgataaa ctcaagaaa aaaattatt 
      gaatacgac gacaaactc aatgattta attgaggat 
      aaaaaccgg ataagtgag ctacgaaaa cagttggag 
      caaaaagag cgcgatttt ttaaaacgc aagcacgaa 
      tttgaggac caaagagcg aattttgag tacgaagcg 
      gacattgaa cgaaaaaaa cttgcaaaa gaacgacag 
      gcgtttcaa atgttcatg aaagagtca caaaatcgc 
      ccaaacaaa aaagaacgg caagaaata agcaacttg 
      aagcaagaa gtggccgat ttgagtgaa actttaaag 
      ctaaaagag tcaaaaaac ggaatgacc caagcccgc 
      cttcgcaac caaatcaaa caactcgaa aaggaaaat 
      tcgcaactc aagactgaa attgaaaaa ttaacgaaa 
      gaaaacgcc aaactttca gcaacgcaa aaactgaac 
      agaaaatca tccgattcg aaaattttg cacgaaatc 
      aacaaaaac ataactaaa ctaacaaac caggcaaaa 
      aatcacgaa aaaaatgta atgctggat cagagtgag 
      tcgtttctg gtcatggca ccccctcta gcggacgaa 
      tgcaacaac ggcggtgcc aaccaatca gaaacgagc 
      gcctttaac tccaaccaa tcacaagtg acgtacccc 
      aacggcaat attaaaaca gtgtcaccc gatggtaac 
      tttataact gttaaattc ttcaatggt gaccgccag 
      gaaactaat ttattggac ggaaccgtt aaatatttt 
      ttcaccaat cgtaacatc acccaaact acgtttgcc 
      gacggtttg gaagtagtc gaatttcct gagggggtt 
      attgagagg cgttttccg gacggcagg tgcgaaatt 
      acgctccca gatggcgct gtgcaaaca acgctacct 
      gacggcggt acggaaact aagtacacc gacggctcg 
      attgtcaaa attgcgcca aacggcgac aaggttttg 
      cttctagcc aatgggcaa aaggaaatc gagacaaaa 
      gaatacaag agtcgcgag tatcccgat ggtactgtt 
      aaaattttg taccgagat gggacccag gaaacgaag 
      tatgccaat gggcgtgtg aggattaag gattctaac 
      gggaaattg gttctagac acgcattta tga
    mRNA sequence
    • >TC015197 RA
      atttaaatt taaatcgct gtcaaccgt ccctaacct 
      taaaaaaaa tgccacttt caccaaacc tcctccaac 
      gcttggccg aactcaaaa aatggcaag aaagccacg 
      aggatttac tcaaaaaaa caattatca acaccagtg 
      caaacgcga ccacattat cggactacc aaaccattg 
      acggtcttt cagcctttg actcctcaa acgaacact 
      cccgtaaaa actgggaga gtcaggtaa tttccccca 
      cgaaaacct tccacgaac tacttgaag aaaaactcg 
      ctcttgatc cgggccctg gggctgcgc cccctaaac 
      cgaaacgcc cctttttaa aaaaggggg ccggtttgg 
      agcgtttcc gcatgaaaa cgcgcccca aattgatta 
      aaaaatcga aaggggaga aagaaaatg ataatgtga 
      ctcctctga gggcccccg atttggtgg tgaggccca 
      aggccacgt gggggcccc ccggcgagc aaacactgt 
      acgagaagg cgctagaga aggagttga ggatttttg 
      aggctttgg aggaaaagg ccgagaata gtagctttt 
      gttcaacga attctagcg ttgtgagga ttttgtcaa 
      gcactccga gtaaaaaag ggggcgatg atgtgatag 
      agactgagt tggagcgca aagacattg cggagattt 
      tattgaggc ttaagaatt tggccgagg gacggatga 
      agccccccg atgtaccct cgattattg aggcttctg 
      atgaggaca agtggacca gtagtgagg tttcaagtg 
      tctcatcgg gggatttag aggagactc tgacagccc 
      caaaacgtg atataggag ttgggactg aggcaagca 
      atgattgtg caatttgtg gtgataaac tcaagaaaa 
      aaattattg aatacgacg acaaactca atgatttaa 
      ttgaggata aaaaccgga taagtgagc tacgaaaac 
      agttggagc aaaaagagc gcgattttt taaaacgca 
      agcacgaat ttgaggacc aaagagcga attttgagt 
      acgaagcgg acattgaac gaaaaaaac ttgcaaaag 
      aacgacagg cgtttcaaa tgttcatga aagagtcac 
      aaaatcgcc caaacaaaa aagaacggc aagaaataa 
      gcaacttga agcaagaag tggccgatt tgagtgaaa 
      ctttaaagc taaaagagt caaaaaacg gaatgaccc 
      aagcccgcc ttcgcaacc aaatcaaac aactcgaaa 
      aggaaaatt cgcaactca agactgaaa ttgaaaaat 
      taacgaaag aaaacgcca aactttcag caacgcaaa 
      aactgaaca gaaaatcat ccgattcga aaattttgc 
      acgaaatca acaaaaaca taactaaac taacaaacc 
      aggcaaaaa atcacgaaa aaaatgtaa tgctggatc 
      agagtgagt cgtttctgg tcatggcac cccctctag 
      cggacgaat gcaacaacg gcggtgcca accaatcag 
      aaacgagcg cctttaact ccaaccaat cacaagtga 
      cgtacccca acggcaata ttaaaacag tgtcacccg 
      atggtaact ttataactg ttaaattct tcaatggtg 
      accgccagg aaactaatt tattggacg gaaccgtta 
      aatattttt tcaccaatc gtaacatca cccaaacta 
      cgtttgccg acggtttgg aagtagtcg aatttcctg 
      agggggtta ttgagaggc gttttccgg acggcaggt 
      gcgaaatta cgctcccag atggcgctg tgcaaacaa 
      cgctacctg acggcggta cggaaacta agtacaccg 
      acggctcga ttgtcaaaa ttgcgccaa acggcgaca 
      aggttttgc ttctagcca atgggcaaa aggaaatcg 
      agacaaaag aatacaaga gtcgcgagt atcccgatg 
      gtactgtta aaattttgt accgagatg ggacccagg 
      aaacgaagt atgccaatg ggcgtgtga ggattaagg 
      attctaacg ggaaattgg ttctagaca cgcatttat 
      gatattatt tattgtatt atgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY