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Gene information
General information
TC014947 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7500743..7502404
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014947
    iBeetle screen iB_06342

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: bad quality of preparation,
    Pupal morphology
    remarks: bad quality of preparation,
    Adult morphology
    remarks: bad quality of preparation,
    Fertility
    egg production: number no/almost no eggs -
    mature egg not present - Number of animals showing the phenotype: 4

    Comment: To be repeated due to preparation errors.
    remarks: bad quality of preparation,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014947 RA
      MDQNVIKVG DFVIVQRQG YTKLHKLKA HGTLSLGSF 
      TVELDNIVG EKYGATFQM KNFPGNRKL FTLEKVDEM 
      PGITSINIE KSGLDNRNI TDDGQSQNL TTEDISKLK 
      SEELNSNSI VEKLINNSK TFNSKTEYS QEKYIKKKE 
      KKYFEYVQI RRPTIRLLA TMFYRQDPS KTLGIRIDD 
      LSQILTYSN IQSQGTHLL YDSGTSGLL PAALLHQLG 
      GGSPAHLIH LHPGNECQK SALVAMNFP PEQLNRCIN 
      VNLYSVLRC YYQQKETFD DPPASKKMK IEEGAVPKK 
      PNWQMENER ACQLLNKGV DSLIIASKE HPNALVSQL 
      AQFLTGGRN LIVFNQSRE PLQDLYLHL KEKLDFIHI 
      KIVTNFMRN YQVLANRTH PEVNMTTGG YILTAFKLK
    CDS sequence
    • >TC014947 RA
      atggatcaa aatgtgata aaagtgggt gattttgtg 
      atagtgcaa cgtcagggg tacacaaaa ctacacaaa 
      ttgaaggct catgggacc ctctcgctt ggtagtttc 
      accgtcgaa ttggataac atcgtgggg gaaaaatat 
      ggggcaact ttccaaatg aagaatttt ccaggcaat 
      cggaagctt ttcacgctt gaaaaagtg gacgaaatg 
      cccggaatc acgtcgatt aacatcgag aaaagtggc 
      ctcgacaat cgtaatata accgacgat ggacaatca 
      caaaatctc acaactgaa gatatttcg aagctcaaa 
      agtgaggaa cttaactcg aattcaatc gttgaaaaa 
      ctgatcaat aattcgaaa acgttcaac tcgaagact 
      gaatacagc caggagaaa tacatcaaa aagaaagaa 
      aaaaaatat ttcgaatac gtccagata aggcggccc 
      acaattcgc ctgttggca acaatgttc taccgacaa 
      gacccgagc aaaacgctc ggaatccga atcgatgac 
      ttatcccaa atcctgact tattcaaac atacaatca 
      caaggaacc cacctattg tatgatagt ggcacttcg 
      gggctcctc ccagccgct ttgctccac caattaggg 
      ggcggaagc cccgcccac ttgatacac ctacacccg 
      gggaacgaa tgccagaaa agtgccctc gttgctatg 
      aacttccct cccgaacaa ctcaatcgt tgcatcaac 
      gttaactta tattcagta ttacgttgt tactaccaa 
      cagaaagaa acatttgac gacccccct gcgagtaaa 
      aaaatgaaa atcgaggag ggggcggtg ccaaagaaa 
      cccaactgg caaatggag aacgagcgg gcgtgtcaa 
      ctactcaac aaaggcgtg gattcgctt atcatcgct 
      tcaaaagaa cacccaaac gccctcgtc tcgcaattg 
      gcccaattc ttgaccggt gggcgtaac cttatcgtt 
      ttcaaccaa tcacgcgag ccccttcaa gacttatac 
      ctccacctt aaggagaaa ctcgacttt attcatata 
      aaaatcgtg actaacttt atgcgtaat tatcaagtg 
      ttggccaat aggacgcat ccagaagtg aacatgaca 
      accggtggt tacatttta acggctttt aaattgaaa 
      tag
    mRNA sequence
    • >TC014947 RA
      ctagtaacg tttgataac cccaaagta cataacctc 
      aaaaatcaa gcatggatc aaaatgtga taaaagtgg 
      gtgattttg tgatagtgc aacgtcagg ggtacacaa 
      aactacaca aattgaagg ctcatggga ccctctcgc 
      ttggtagtt tcaccgtcg aattggata acatcgtgg 
      gggaaaaat atggggcaa ctttccaaa tgaagaatt 
      ttccaggca atcggaagc ttttcacgc ttgaaaaag 
      tggacgaaa tgcccggaa tcacgtcga ttaacatcg 
      agaaaagtg gcctcgaca atcgtaata taaccgacg 
      atggacaat cacaaaatc tcacaactg aagatattt 
      cgaagctca aaagtgagg aacttaact cgaattcaa 
      tcgttgaaa aactgatca ataattcga aaacgttca 
      actcgaaga ctgaataca gccaggaga aatacatca 
      aaaagaaag aaaaaaaat atttcgaat acgtccaga 
      taaggcggc ccacaattc gcctgttgg caacaatgt 
      tctaccgac aagacccga gcaaaacgc tcggaatcc 
      gaatcgatg acttatccc aaatcctga cttattcaa 
      acatacaat cacaaggaa cccacctat tgtatgata 
      gtggcactt cggggctcc tcccagccg ctttgctcc 
      accaattag ggggcggaa gccccgccc acttgatac 
      acctacacc cggggaacg aatgccaga aaagtgccc 
      tcgttgcta tgaacttcc ctcccgaac aactcaatc 
      gttgcatca acgttaact tatattcag tattacgtt 
      gttactacc aacagaaag aaacatttg acgaccccc 
      ctgcgagta aaaaaatga aaatcgagg agggggcgg 
      tgccaaaga aacccaact ggcaaatgg agaacgagc 
      gggcgtgtc aactactca acaaaggcg tggattcgc 
      ttatcatcg cttcaaaag aacacccaa acgccctcg 
      tctcgcaat tggcccaat tcttgaccg gtgggcgta 
      accttatcg ttttcaacc aatcacgcg agccccttc 
      aagacttat acctccacc ttaaggaga aactcgact 
      ttattcata taaaaatcg tgactaact ttatgcgta 
      attatcaag tgttggcca ataggacgc atccagaag 
      tgaacatga caaccggtg gttacattt taacggctt 
      ttaaattga aatagtgac tcacctgtg tgaaaaact 
      gttgcaaca ttattttat tcgtgggcg ggattaaat 
      actcgagga attcctgta aatattcgt attgtttgg 
      cgagaaaga tgacacttt ccacgccca cgatacgtt 
      cagccaatc cgtgcaagt gttcaggtt tggtgaggt 
      ccaccattg gtgagaggg cgggtttta ggattttgg 
      gcggggttt cgtcagtgg gcggttcct cgaattcgg 
      tgactagat tgtcggaga tttttttca acgtttggg 
      tgagttcgg gcgtgtaga gagagttgc tagcgacgg 
      tctaaaata attgatttt ctactaaaa aattgtaaa 
      taggaaatt ttattttcg atgagaaca cgtcaaatt 
      tgaaaagtg tttataaaa taataataa agtattgtt 
      gtattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY