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Gene information
General information
TC014920 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8082683..8084174
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014920
    iBeetle screen iB_06341

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014920 RA
      MEPQITQRG QFFVLSNYV PASSQFKCH ESITVTTQG 
DFTFIENLL PLVERWQGP VSVALYAPG TDFDRTVAS 
IRYLRECTN HIIEKFVTF HIFFETEHT PETVSWPEG 
AFDCAQNPP FRESSAQTY KAENDLVYP INVGRNVAR 
EMATTHFVL ALDIELFPN PGFIPKFLD MIARDEPPL 
DRPYPRVFP LPVFEIKSG EKIPENKSQ LREMLDKNL 
AIYFHKDIC YSCHKIPKF DQWKRLNES QNFGVFRVV 
KRIHEHKHW EPFYVGTNA EPLYDERLS WEGRRNKMT 
NGFILCVLD YDLMVLDNA FLVHRPGIK EKSAKNERR 
SKLVKETNK LIEQRILPE MKVLYGFKD GCIV
    CDS sequence
    • >TC014920 RA
      atggagccc caaatcacc caaaggggc caatttttc 
gtcctgtcc aactacgtg ccagcttca agccaattc 
aaatgccac gaaagcatc acagtgacc acacaaggc 
gattttact tttatcgaa aatttgcta ccactggtg 
gagaggtgg caggggcct gttagtgtc gccttgtac 
gcccctgga accgacttc gaccgaaca gtcgcttcg 
atccgctac ttgcgcgaa tgcaccaat cacatcatt 
gaaaaattt gtcacgttt cacattttc ttcgagact 
gaacatacg ccagaaacg gtttcctgg cctgagggg 
gcctttgac tgcgcccaa aaccccccg tttcgggaa 
agttcggcc caaacgtac aaagccgaa aacgatctc 
gtctacccg atcaatgtg ggtcgaaac gttgcgaga 
gaaatggca acaacgcac tttgtccta gcgctggac 
atcgaactg ttccccaat cccggattt atcccgaaa 
ttcctggac atgatcgcc cgggacgag ccgcccctg 
gaccgtccc taccctcga gtgttccct ttgccagtt 
ttcgagatc aaatcgggg gagaaaatc cccgaaaac 
aagtcacaa cttcgcgaa atgttggat aaaaatctt 
gcaatttac tttcacaag gatatttgt tactcctgt 
cacaaaatc ccgaagttc gatcaatgg aaacggtta 
aatgagtcg caaaatttt ggggttttt cgggtggtg 
aagaggatt catgaacac aaacactgg gaaccgttt 
tatgttggt acgaatgcg gagccgctc tatgacgaa 
aggttaagt tgggaagga aggaggaat aaaatgacc 
aatgggttc attttgtgt gtgcttgac tacgacttg 
atggttctg gataatgcc tttttggta cacaggcca 
gggattaag gaaaagtcc gccaaaaat gaaaggagg 
agtaagttg gtcaaggaa accaataaa ctgatcgag 
caacgaatt ttgccagaa atgaaggtt ttgtacggg 
tttaaagac ggttgcatt gtttga
    mRNA sequence
    • >TC014920 RA
      gatctccag tcaaaaatg tttcacaat tattgttat 
tttcgccac tctacttac gatatatct aatttcgta 
cagtgtaag aaaaaaaag tgattctac tagtgacca 
caaacccac attaattaa aaattcccc acttttggg 
gactctagc caaggtaaa aaaaattac caaatgttt 
cgacaaaac catggagcc ccaaatcac ccaaagggg 
ccaattttt cgtcctgtc caactacgt gccagcttc 
aagccaatt caaatgcca cgaaagcat cacagtgac 
cacacaagg cgattttac ttttatcga aaatttgct 
accactggt ggagaggtg gcaggggcc tgttagtgt 
cgccttgta cgcccctgg aaccgactt cgaccgaac 
agtcgcttc gatccgcta cttgcgcga atgcaccaa 
tcacatcat tgaaaaatt tgtcacgtt tcacatttt 
cttcgagac tgaacatac gccagaaac ggtttcctg 
gcctgaggg ggcctttga ctgcgccca aaacccccc 
gtttcggga aagttcggc ccaaacgta caaagccga 
aaacgatct cgtctaccc gatcaatgt gggtcgaaa 
cgttgcgag agaaatggc aacaacgca ctttgtcct 
agcgctgga catcgaact gttccccaa tcccggatt 
tatcccgaa attcctgga catgatcgc ccgggacga 
gccgcccct ggaccgtcc ctaccctcg agtgttccc 
tttgccagt tttcgagat caaatcggg ggagaaaat 
ccccgaaaa caagtcaca acttcgcga aatgttgga 
taaaaatct tgcaattta ctttcacaa ggatatttg 
ttactcctg tcacaaaat cccgaagtt cgatcaatg 
gaaacggtt aaatgagtc gcaaaattt tggggtttt 
tcgggtggt gaagaggat tcatgaaca caaacactg 
ggaaccgtt ttatgttgg tacgaatgc ggagccgct 
ctatgacga aaggttaag ttgggaagg aaggaggaa 
taaaatgac caatgggtt cattttgtg tgtgcttga 
ctacgactt gatggttct ggataatgc ctttttggt 
acacaggcc agggattaa ggaaaagtc cgccaaaaa 
tgaaaggag gagtaagtt ggtcaagga aaccaataa 
actgatcga gcaacgaat tttgccaga aatgaaggt 
tttgtacgg gtttaaaga cggttgcat tgtttgatt 
tgaattttt ttgtgattt tctttcatt ttcatgatt 
taatagacg tattaacga acgcaacgt catcatgtc 
ttttggcaa tttatactt tattacttg tcactagtc 
actagtcac tagtgggtt atttttgaa tttctataa 
atatgcaat cgataattg caataccaa taaaaaaaa 
tgtaatcaa
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