Status
Please wait ...
Gene information
General information
TC014919 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8089946..8124310
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014919
    iBeetle screen iB_06340

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014919 RA
      MVSSEDQKK QLRGAIINN RLEEVKNII TKGYDLNSP 
      LFQWTPLTY AITCKNLEM IQLILDNQA DINFESPQK 
      YVPLYTAID YNCSLEVIQ LLINRGADI NQLGRSFFN 
      DKINTPLSR AIQNNNLQA AHLLVENGA DVHKPNPDG 
      TTAGSLAHD LDRADFIKE KPPQENNDS LKGCTPLTA 
      AILSGKLDE AKLLLESGV NSCKPDSNK QTPLVAAVR 
      CKNLEAVAL LLCYKANLT IPTAFLEAV NLQCTEIVK 
      YLLENGAKI NKTYRDGST PFIKAIETE NLAIIEELL 
      HTGAQVDLA NREGKTPLA VAASKRNIL IVNHLIEAG 
      ASVSLARQE LEEFANDDE DFDLYYQDL VERGEQIVA 
      PVSNRGLPL GANATAD
    CDS sequence
    • >TC014919 RA
      atggtgtct tcggaggac cagaaaaag caactgagg 
      ggggcaata ataaacaac agactggaa gaagtcaag 
      aacatcatt accaaagga tatgatcta aactcccct 
      ctctttcaa tggactcca cttacgtac gccattact 
      tgcaaaaat ttggaaatg attcagttg atattagat 
      aaccaggct gatataaat tttgaaagt ccccaaaaa 
      tatgtgcct ttatacact gccattgac tacaactgt 
      tcactcgaa gtcattcaa ctcttaatc aaccgcgga 
      gctgatatc aaccaactt ggacgcagt ttcttcaat 
      gacaaaatc aacactccc ctatcacga gcaatccag 
      aacaataat ttgcaagcc gctcattta ctggtcgaa 
      aatggagct gacgtccat aagccaaac ccagatgga 
      acaactgct gggtcactt gcccacgac ctagaccgt 
      gcagatttc atcaaagaa aagccacca caagaaaac 
      aatgacagt ttaaaaggt tgcacgcct ctgactgca 
      gccattctg tccgggaaa ctggacgaa gcaaagcta 
      cttctagaa tcgggagtg aattcctgc aagcctgac 
      tccaacaaa caaacccca cttgtggcc gcagtccgg 
      tgtaaaaac ctcgaagct gtggcactc ctactgtgt 
      tacaaagcg aatttaaca atcccgact gcgtttttg 
      gaggctgtt aacttacag tgcaccgaa attgtcaaa 
      tatttgtta gaaaatggg gccaaaatc aataaaacg 
      taccgcgat ggttccacg ccgtttatc aaagcgata 
      gaaacggaa aatttggca atcattgag gagcttttg 
      cacactggg gcccaagtg gacctggct aatagagaa 
      ggaaagact cctttggct gtcgcagcg agtaaaaga 
      aatattctc attgtgaat catttgatt gaggcggga 
      gctagcgtt agtttggca cgccaagag cttgaagaa 
      tttgcaaat gatgatgaa gattttgac ttgtattat 
      caagatttg gtggagcga ggagaacag atcgtggct 
      ccagtgtct aacagaggt ttgccactt ggagccaac 
      gccacggca gactaa
    mRNA sequence
    • >TC014919 RA
      ctttaagtt ggtttgcta ggggtttcg cacgattaa 
      gtccactgg cactgctgt tgtctcctt atcttatca 
      tttgttctg tggtcaact aaacaaaat ggtgtcttc 
      ggaggacca gaaaaagca actgagggg ggcaataat 
      aaacaacag actggaaga agtcaagaa catcattac 
      caaaggata tgatctaaa ctcccctct ctttcaatg 
      gactccact tacgtacgc cattacttg caaaaattt 
      ggaaatgat tcagttgat attagataa ccaggctga 
      tataaattt tgaaagtcc ccaaaaata tgtgccttt 
      atacactgc cattgacta caactgttc actcgaagt 
      cattcaact cttaatcaa ccgcggagc tgatatcaa 
      ccaacttgg acgcagttt cttcaatga caaaatcaa 
      cactcccct atcacgagc aatccagaa caataattt 
      gcaagccgc tcatttact ggtcgaaaa tggagctga 
      cgtccataa gccaaaccc agatggaac aactgctgg 
      gtcacttgc ccacgacct agaccgtgc agatttcat 
      caaagaaaa gccaccaca agaaaacaa tgacagttt 
      aaaaggttg cacgcctct gactgcagc cattctgtc 
      cgggaaact ggacgaagc aaagctact tctagaatc 
      gggagtgaa ttcctgcaa gcctgactc caacaaaca 
      aaccccact tgtggccgc agtccggtg taaaaacct 
      cgaagctgt ggcactcct actgtgtta caaagcgaa 
      tttaacaat cccgactgc gtttttgga ggctgttaa 
      cttacagtg caccgaaat tgtcaaata tttgttaga 
      aaatggggc caaaatcaa taaaacgta ccgcgatgg 
      ttccacgcc gtttatcaa agcgataga aacggaaaa 
      tttggcaat cattgagga gcttttgca cactggggc 
      ccaagtgga cctggctaa tagagaagg aaagactcc 
      tttggctgt cgcagcgag taaaagaaa tattctcat 
      tgtgaatca tttgattga ggcgggagc tagcgttag 
      tttggcacg ccaagagct tgaagaatt tgcaaatga 
      tgatgaaga ttttgactt gtattatca agatttggt 
      ggagcgagg agaacagat cgtggctcc agtgtctaa 
      cagaggttt gccacttgg agccaacgc cacggcaga 
      ctaattcga acattagag gctgtgcat ctttatagc 
      aataatttt tgggttttc tcaatgtaa atttaataa 
      atgtttcta caagtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY