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Gene information
General information
TC014880 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9213549..9220116
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014880
    iBeetle screen iB_06339

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014880 RA
      MEAKNQATK GKRVHPKEK SNFLSNLLF CWQIGIILK 
      GRKKELTED ELYGPLKSQ QSLKLGEKL EKHWNEEKT 
      RKKPSLARA LIKSFGMEF VLYGVLLFP FDFALTLLQ 
      PILLRKLLN YFDSNRENI PETQAYCYA LVIVLASLM 
      YLTSFHVTY LGLYNLSLK VRVACSSLV YKKILTLRR 
      ESFQNLTSG QIINLLSND IHRIDLVLP TLHFLWSAP 
      LKLVLATVY LTITFGFTA TLGIFGILF FVIAQLLVA 
      RKFSQVKQK LGPKTDHRI RLFNDIIRG IQVIKMCTW 
      EQSFSNYIS EVRRSETNQ LSTANHFRF VCAVFKVYV 
      SKISVFLCI LGSVLTNTA LTPQYVYAL MTIYENLKT 
      TATTRLPFA LIRMTETKV TVQRIQNFL LVGCPNNVP 
      RDIIKFLKP KMPTIQIHN AWIELNGVT LLHDITFHA 
      SPGDLIGVV GSAGSGKTT LLHTILSEV SPAKGMIEV 
      EGIISYASQ EPWIFSGSV RQNILFGEE FNPAKYSQV 
      VKVCALEYD LTQLPFGDN TLVGERGAM LSGGQKARI 
      NLARAVYKH ADIYLLDDP LSAVDTHVA RHIFDECIV 
      KYLKGKCVV LVTHQVQYL KNTTRIYQL DKGRVDKVG 
      TFDVLGFED KSSGGKNDT KSVTLAEFQ APSIVKEER 
      GSGSGAKVY RDYFLNATG VTITVSVFL LFALNTFLG 
      YLIDIFITF WVNSKIDID TNDKLYIYG FLVLLLVFG 
      THIATWFFV KYTVVISKF LHDVFYQGV INSPIKFFN 
      DNSSGRILN RYSTDLGSA DEVIPLFLL ELLIKIFLV 
      IGVSIIIPI LNFWMVIPS ILLYIIFFT YMYLHQPAN 
      KSMRRTEGV VTSPVISHM ASTLQGLTT IQALKAQQI 
      LLQEFSAKL DVQTSACYM SKAIFCTLA FWADLTCTI 
      YTGVALFSF FFFTGNYVG VIGLAFTTS IIFGETAQM 
      CMKDWSDID SNMTSVERV MDYSRLAPE ADEGSFIPE 
      DPWPDQGII VFDRVSMKY SDNFWALNQ TSFSIIAGE 
      KIGIVGRTG AGKSSLISA LFRLFDFEG TITIDGVDT 
      KQLPLHVLR SKISIIPQE PVIFVGSLR KNLDPFDEF 
      SDADLWNAL EEVELKKHF LETGLDSPV SEGGSNFSV 
      GERQLLCLV RTILRKAKI IVLDEATSN VDWKTDEMI 
      QSTVRRKFE GCTVLTIAH RLKSVLDSD KILVMESGS 
      VVEFGCPKD LLKDTEGYF YKLVQKSKP VNIL
    CDS sequence
    • >TC014880 RA
      atggaggcc aagaaccaa gcaaccaaa ggaaaaagg 
      gttcacccg aaagaaaaa tccaacttc ctatcaaac 
      ctcctcttc tgttggcag atcggtata atactcaaa 
      gggcgcaaa aaggaacta acagaagat gagctctac 
      ggaccactt aagtcgcaa cagtcacta aaattgggc 
      gaaaagtta gaaaaacac tggaatgag gaaaagact 
      cgcaaaaaa ccgtccctg gccagggct ttgatcaag 
      agttttgga atggaattt gtcctctat ggtgttttg 
      ctgttccct tttgatttt gccttgacg ttattacaa 
      cctattctg ttacggaaa cttttgaat tattttgac 
      tcaaaccgg gaaaacatc cccgaaact caggcctat 
      tgctatgcc cttgtgata gtactcgca tcactaatg 
      tatctgact tcgttccat gtaacctac ttgggacta 
      tacaacttg agtttaaag gtccgggtc gcttgtagt 
      tcactggtg tacaagaaa attctaact ttgcggagg 
      gaaagtttc caaaatctg acttcgggc caaattatc 
      aatttgctc tcgaatgac atacaccgt atcgacttg 
      gtactacca acactgcat ttcttatgg agtgcccct 
      ttgaaacta gttttggcc actgtttac ttaaccatc 
      actttcgga ttcactgca acgttaggg attttcgga 
      atcttgttt tttgtaata gcgcaatta ctcgttgcc 
      aggaagttc tcccaagtt aagcaaaaa ctgggcccg 
      aaaacggac cacaggatt cgcttgttc aatgacatc 
      attcgaggt atccaagtc atcaaaatg tgcacttgg 
      gaacaatcc ttctccaac tacattagt gaggtccga 
      aggtcagaa accaaccaa ctttccacc gccaaccat 
      ttccggttt gtttgcgcg gtctttaaa gtctacgtt 
      tccaaaatc tccgtcttc ctttgcatc cttggaagt 
      gttctaacc aacacagcc ttaacccct caatatgtg 
      tatgcccta atgacaata tacgaaaat ttgaaaacc 
      acagccaca acacgtctc cctttcgct ttaatacga 
      atgacagaa accaaagtc acggttcaa cgaatccaa 
      aacttttta cttgtcggg tgtccaaac aacgtcccg 
      agggacata atcaaattc ctcaaaccc aaaatgccc 
      acaattcaa atccataat gcctggatc gaactcaac 
      ggtgtgact ctacttcac gacatcaca tttcatgcc 
      tcacctggt gatttgatt ggagtggtc gggagtgca 
      ggaagtggc aaaaccact ctcctgcat actattcta 
      tcagaggtt agcccggcc aaaggcatg atcgaagtc 
      gaaggcatt atctcgtat gcctcacaa gaaccgtgg 
      attttttcg ggaagtgtg aggcaaaat attttgttt 
      ggtgaggag ttcaatcct gccaagtat tcgcaagtt 
      gtgaaagtt tgtgcgttg gagtacgac ttgactcaa 
      ctccccttt ggtgacaat acactagtt ggggaaagg 
      ggtgccatg ttaagtggg gggcagaaa gctaggatt 
      aatttggct cgcgctgta tacaaacac gcagatata 
      tatcttcta gatgatcca ttatctgca gttgataca 
      cacgtagcc aggcatatt tttgacgaa tgtatcgtc 
      aagtatttg aaggggaag tgtgtggtt ctggtgaca 
      caccaggtg caatatttg aagaacaca acacggatt 
      tatcaactg gataaagga cgggttgat aaagttggt 
      acatttgat gtcttgggt ttcgaggat aagtcaagt 
      ggaggtaaa aacgatacg aaaagtgtg actttggcg 
      gagttccaa gccccttca attgtcaaa gaagaacga 
      ggaagtgga agtggggct aaagtatat cgcgactat 
      tttcttaac gcaactggg gttactata accgtatca 
      gtattcctt ctattcgca ctcaacacg ttcctcggt 
      tatttgatt gatattttc atcacgttt tgggtgaac 
      tcaaaaatt gatattgac accaatgac aaattatac 
      atatatgga ttcttagta ctacttctt gttttcggg 
      actcatatc gcgacgtgg tttttcgtc aagtacaca 
      gtcgtgata tcaaaattc ctccacgat gtgttctat 
      caaggtgtt atcaatagc ccgatcaaa ttttttaat 
      gacaactcc tcgggacgt atcctcaat cgttactcg 
      accgatttg ggatcagct gacgaagtt atccctttg 
      ttccttctg gaactccta attaaaatt ttcttggtc 
      atcggagta tccataata atcccaata ctcaatttc 
      tggatggtg ataccatcc atacttttg tatattatc 
      tttttcacg tacatgtat ttgcaccaa ccggcaaac 
      aaaagtatg aggcgtacc gaaggagtt gtaacaagt 
      cctgtgata agccacatg gcatcaacc ctgcaaggc 
      ctaaccaca atccaggcc ttgaaagcc caacaaatc 
      ctcctacaa gagttcagt gctaaatta gacgtccaa 
      acttcggct tgttacatg agcaaggct atattttgc 
      actttggcg ttttgggcc gacttgacc tgtaccata 
      tacacagga gtggcgctt tttagcttc tttttcttc 
      acagggaat tacgtggga gtcatcggt ttggccttc 
      acaacatcc ataattttc ggcgaaacg gcccaaatg 
      tgtatgaaa gactggagc gacattgac tccaacatg 
      acttcagtc gaacgcgtt atggactac tcgagactt 
      gcccccgaa gcggacgaa ggctcattc ataccggaa 
      gacccatgg cctgatcaa ggcattata gttttcgac 
      cgcgtttca atgaaatac tccgataac ttctgggct 
      ttaaaccaa acttctttc agtattata gcaggtgag 
      aaaatcggg attgttggt agaaccggt gccggaaag 
      tcgtcacta atatcagca cttttccgc ttgtttgat 
      ttcgaggga actatcacc attgatggt gttgacact 
      aaacagttg cctctacat gtcttgcgg tccaaaatc 
      tcgatcatt ccacaagaa ccggttatt tttgtcggc 
      agtcttcgc aagaatttg gaccctttt gacgagttc 
      tccgatgcc gatctgtgg aacgctttg gaagaagtc 
      gaattaaag aaacacttc ctcgaaact gggcttgac 
      agcccagtt tcggaaggt ggttcaaac tttagtgtt 
      ggtgaacga caactgctg tgtctcgtt agaactata 
      ctcaggaaa gccaaaatc atcgttttg gacgaggca 
      acttctaac gttgactgg aaaaccgat gagatgatc 
      cagtcaact gttagaaga aaattcgaa gggtgtacg 
      gtactaact atagcacat cggctaaaa agtgtgcta 
      gattcggac aagattttg gtaatggag tcgggatca 
      gtggtggag tttggatgt ccgaaggac ctactcaag 
      gatacagaa ggttatttt tacaaactt gtacaaaaa 
      agcaaacct gtaaatata ttataa
    mRNA sequence
    • >TC014880 RA
      gtctataaa tttcttaaa ataagcatg gaggccaag 
      aaccaagca accaaagga aaaagggtt cacccgaaa 
      gaaaaatcc aacttccta tcaaacctc ctcttctgt 
      tggcagatc ggtataata ctcaaaggg cgcaaaaag 
      gaactaaca gaagatgag ctctacgga ccacttaag 
      tcgcaacag tcactaaaa ttgggcgaa aagttagaa 
      aaacactgg aatgaggaa aagactcgc aaaaaaccg 
      tccctggcc agggctttg atcaagagt tttggaatg 
      gaatttgtc ctctatggt gttttgctg ttccctttt 
      gattttgcc ttgacgtta ttacaacct attctgtta 
      cggaaactt ttgaattat tttgactca aaccgggaa 
      aacatcccc gaaactcag gcctattgc tatgccctt 
      gtgatagta ctcgcatca ctaatgtat ctgacttcg 
      ttccatgta acctacttg ggactatac aacttgagt 
      ttaaaggtc cgggtcgct tgtagttca ctggtgtac 
      aagaaaatt ctaactttg cggagggaa agtttccaa 
      aatctgact tcgggccaa attatcaat ttgctctcg 
      aatgacata caccgtatc gacttggta ctaccaaca 
      ctgcatttc ttatggagt gcccctttg aaactagtt 
      ttggccact gtttactta accatcact ttcggattc 
      actgcaacg ttagggatt ttcggaatc ttgtttttt 
      gtaatagcg caattactc gttgccagg aagttctcc 
      caagttaag caaaaactg ggcccgaaa acggaccac 
      aggattcgc ttgttcaat gacatcatt cgaggtatc 
      caagtcatc aaaatgtgc acttgggaa caatccttc 
      tccaactac attagtgag gtccgaagg tcagaaacc 
      aaccaactt tccaccgcc aaccatttc cggtttgtt 
      tgcgcggtc tttaaagtc tacgtttcc aaaatctcc 
      gtcttcctt tgcatcctt ggaagtgtt ctaaccaac 
      acagcctta acccctcaa tatgtgtat gccctaatg 
      acaatatac gaaaatttg aaaaccaca gccacaaca 
      cgtctccct ttcgcttta atacgaatg acagaaacc 
      aaagtcacg gttcaacga atccaaaac tttttactt 
      gtcgggtgt ccaaacaac gtcccgagg gacataatc 
      aaattcctc aaacccaaa atgcccaca attcaaatc 
      cataatgcc tggatcgaa ctcaacggt gtgactcta 
      cttcacgac atcacattt catgcctca cctggtgat 
      ttgattgga gtggtcggg agtgcagga agtggcaaa 
      accactctc ctgcatact attctatca gaggttagc 
      ccggccaaa ggcatgatc gaagtcgaa ggcattatc 
      tcgtatgcc tcacaagaa ccgtggatt ttttcggga 
      agtgtgagg caaaatatt ttgtttggt gaggagttc 
      aatcctgcc aagtattcg caagttgtg aaagtttgt 
      gcgttggag tacgacttg actcaactc ccctttggt 
      gacaataca ctagttggg gaaaggggt gccatgtta 
      agtgggggg cagaaagct aggattaat ttggctcgc 
      gctgtatac aaacacgca gatatatat cttctagat 
      gatccatta tctgcagtt gatacacac gtagccagg 
      catattttt gacgaatgt atcgtcaag tatttgaag 
      gggaagtgt gtggttctg gtgacacac caggtgcaa 
      tatttgaag aacacaaca cggatttat caactggat 
      aaaggacgg gttgataaa gttggtaca tttgatgtc 
      ttgggtttc gaggataag tcaagtgga ggtaaaaac 
      gatacgaaa agtgtgact ttggcggag ttccaagcc 
      ccttcaatt gtcaaagaa gaacgagga agtggaagt 
      ggggctaaa gtatatcgc gactatttt cttaacgca 
      actggggtt actataacc gtatcagta ttccttcta 
      ttcgcactc aacacgttc ctcggttat ttgattgat 
      attttcatc acgttttgg gtgaactca aaaattgat 
      attgacacc aatgacaaa ttatacata tatggattc 
      ttagtacta cttcttgtt ttcgggact catatcgcg 
      acgtggttt ttcgtcaag tacacagtc gtgatatca 
      aaattcctc cacgatgtg ttctatcaa ggtgttatc 
      aatagcccg atcaaattt tttaatgac aactcctcg 
      ggacgtatc ctcaatcgt tactcgacc gatttggga 
      tcagctgac gaagttatc cctttgttc cttctggaa 
      ctcctaatt aaaattttc ttggtcatc ggagtatcc 
      ataataatc ccaatactc aatttctgg atggtgata 
      ccatccata cttttgtat attatcttt ttcacgtac 
      atgtatttg caccaaccg gcaaacaaa agtatgagg 
      cgtaccgaa ggagttgta acaagtcct gtgataagc 
      cacatggca tcaaccctg caaggccta accacaatc 
      caggccttg aaagcccaa caaatcctc ctacaagag 
      ttcagtgct aaattagac gtccaaact tcggcttgt 
      tacatgagc aaggctata ttttgcact ttggcgttt 
      tgggccgac ttgacctgt accatatac acaggagtg 
      gcgcttttt agcttcttt ttcttcaca gggaattac 
      gtgggagtc atcggtttg gccttcaca acatccata 
      attttcggc gaaacggcc caaatgtgt atgaaagac 
      tggagcgac attgactcc aacatgact tcagtcgaa 
      cgcgttatg gactactcg agacttgcc cccgaagcg 
      gacgaaggc tcattcata ccggaagac ccatggcct 
      gatcaaggc attatagtt ttcgaccgc gtttcaatg 
      aaatactcc gataacttc tgggcttta aaccaaact 
      tctttcagt attatagca ggtgagaaa atcgggatt 
      gttggtaga accggtgcc ggaaagtcg tcactaata 
      tcagcactt ttccgcttg tttgatttc gagggaact 
      atcaccatt gatggtgtt gacactaaa cagttgcct 
      ctacatgtc ttgcggtcc aaaatctcg atcattcca 
      caagaaccg gttattttt gtcggcagt cttcgcaag 
      aatttggac ccttttgac gagttctcc gatgccgat 
      ctgtggaac gctttggaa gaagtcgaa ttaaagaaa 
      cacttcctc gaaactggg cttgacagc ccagtttcg 
      gaaggtggt tcaaacttt agtgttggt gaacgacaa 
      ctgctgtgt ctcgttaga actatactc aggaaagcc 
      aaaatcatc gttttggac gaggcaact tctaacgtt 
      gactggaaa accgatgag atgatccag tcaactgtt 
      agaagaaaa ttcgaaggg tgtacggta ctaactata 
      gcacatcgg ctaaaaagt gtgctagat tcggacaag 
      attttggta atggagtcg ggatcagtg gtggagttt 
      ggatgtccg aaggaccta ctcaaggat acagaaggt 
      tatttttac aaacttgta caaaaaagc aaacctgta 
      aatatatta taaaaaata tccatttat ttgtcctcg 
      ctttaagaa atttgtaca cttaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY