Status
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Gene information
General information
TC014779 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14984362..15007740
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014779
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06337

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014779 RA
      MKVGAWTVD REVLRRDGL VLFSKLIRT KNVEGLQEQ 
      SRPTGTDDR KQKLTKCLT TLDLTSLGV GSCVGTGMY 
      VVAGMVAKN YAGPGVVIS FIIAAIASI FSGACYAEF 
      GVRVPHTTG SAYMYSYVT VGEFIAFVI GWNMILEYL 
      IGTSACACA LSACFNALA NGAIRAAVE KSIFGTMFG 
      RAPDFLAFV ITILMMLLM AAGVKKSLI FNNVLNAIN 
      LAVWVFTMT AGLFYVNSD NWTKHDGFL PHGWSGVFT 
      GAATCFYAF IGFDIIATT GEEATNPKK SIPLAIITS 
      LGIILTAYV TSSMMLTLI IPFNEVDEN SALVEMFGQ 
      VGAYKCKYI VAVGALAGL TVSMFGSMF PMPRIIYAM 
      AQDGLIFKI FSQVWPSTG TPALATFIS GLAAGVAAL 
      FIRLEILVE MMSIGTLLA YTLVSTCVL ILRYQPHST 
      NLVELLPQS LRTPLRGSP TKEGLTQNP NGQALYGNQ 
      LHPDALKQA IDASLGPPI PSPSPSPLP TQTTQRVMV 
      RRVTRSSPD SDDTYGADE QEEEFSMRD DQFLVSDRT 
      ENKFYGTLH GAGSATSTG GVVGSTVGP SLGYIGRRL 
      QAATYLCPA IFPWVDTGP ATEESGMFV MKMVGILYL 
      LIIIFDLMM VCGMHNMSG TLTFFLFLF LFAIIAVLL 
      VISRKPQNR NTLMFMTPG LPFVPAVAI TVNIYLIFK 
      LSILTLVRF TIWMTLGFI MYFYYGIKN SILEEGEGD 
      QNIELTVTD HEKPKYNNT DQTIFSQEQ PTYDWDPKT 
      VWDHQPSWA QPTQQYQVE QQYNVETNN TRSVINRAN 
      PTSGSSLFV NDPNAFPTW DD
    CDS sequence
    • >TC014779 RA
      atgaaggtg ggcgcttgg acggtcgat cgggaggtc 
      ctccggaga gacggcttg gtcctcttc agcaaactc 
      atccgaaca aagaacgtt gagggccta caagaacaa 
      agcagaccg acggggacc gatgaccgc aaacaaaag 
      ctcacgaaa tgtctgacg actctggat ttgacatcg 
      ctgggagtg ggaagctgt gtgggaact ggaatgtat 
      gtggtggca ggaatggtg gcaaaaaac tacgctggc 
      cctggcgtg gtcatcagt ttcatcatt gcagcaata 
      gccagcata ttttcagga gcctgctat gcagagttc 
      ggtgtcagg gtgccacac accaccggc tccgcatac 
      atgtactcg tacgtcacc gtcggagaa ttcatcgcc 
      ttcgtcatt ggctggaac atgatcttg gagtattta 
      attgggaca tcggcatgt gcttgtgca ctcagcgcc 
      tgttttaac gcattggcg aatggggca attagggcg 
      gcagtcgag aagagcatc tttggcaca atgttcggc 
      cgagccccg gattttctg gctttcgtc ataaccatc 
      ttgatgatg ctgctcatg gcagccggg gtgaaaaaa 
      tcgctcata ttcaacaac gtcctcaat gctattaat 
      ctggccgtt tgggtgttt acgatgact gccggcctt 
      ttctacgta aattccgac aactggacc aaacacgac 
      gggtttctg ccgcacgga tggtcaggg gtctttact 
      ggagcagcg acttgcttt tacgccttc atcggcttc 
      gatatcatt gcaacaacg ggcgaagaa gccaccaat 
      ccgaaaaaa tcaatccct ctcgccatt ataacaagt 
      ttgggaatc atcctgacc gcctacgtc accagttcc 
      atgatgctg acattaatc attcccttc aacgaggtc 
      gacgagaat tcggccctg gtcgaaatg ttcggccaa 
      gtgggcgct tacaaatgt aaatacatc gtggcggtg 
      ggtgccttg gccggcctc accgtcagc atgttcggg 
      tccatgttc cccatgcct cggattatt tacgcgatg 
      gctcaagac gggttaatt ttcaaaatt ttttcgcaa 
      gtgtggccg agcacggga accccggct ttagcaacg 
      ttcattagt gggttggca gcaggtgtg gctgctctt 
      tttataagg ttagaaatc ctcgtagaa atgatgtcg 
      ataggtacc cttttagct tatacgtta gtatcgaca 
      tgtgtccta atattgagg tatcaaccc cactcgaca 
      aatctagta gagttgtta cctcaaagt ttgagaact 
      ccattgaga ggatctcca acgaaagaa ggattgact 
      caaaatccg aacggacaa gccttgtac gggaaccaa 
      ctccaccca gacgctttg aaacaagcg attgacgct 
      agcttagga ccgccgatt ccttcaccg tcgccctcg 
      ccgctgccc acccaaacc acgcagagg gtcatggtg 
      aggagggtc accaggagc tcaccagac agtgacgac 
      acttacgga gcagacgaa caagaagaa gagttctcc 
      atgcgagac gaccagttc ctagtttca gaccggacg 
      gaaaacaaa ttttacgga actctgcac ggcgccggc 
      agcgcgacc agcacgggc ggggttgtg gggtccacc 
      gtggggccc agcctgggc tacatcggc cgccggtta 
      caggctgcg acttattta tgccctgcg atattccca 
      tgggtggac accggccct gccactgaa gagtcgggg 
      atgtttgtg atgaaaatg gtgggaata ttgtattta 
      ttaatcata attttcgat ctgatgatg gtctgcggg 
      atgcacaac atgtcaggg actttaaca ttttttctt 
      ttccttttc ttgttcgcc attattgcg gttttatta 
      gtcatatcg aggaagcca caaaatcgc aacactttg 
      atgttcatg acaccaggt ttgcctttc gttcctgcc 
      gtcgcgatc accgttaac atctaccta attttcaaa 
      ctgtcgatc ctaactcta gtcagattc acgatttgg 
      atgactttg ggattcatc atgtatttc tactacggg 
      attaagaat agcattctc gaggaaggg gaaggagac 
      caaaatatc gaactgact gtgaccgac cacgagaag 
      cccaaatac aacaacaca gatcagacg attttcagc 
      caagaacaa cccacatat gactgggat cccaagacc 
      gtttgggac catcagccc tcatgggcc caacccacc 
      cagcaatac caagtagaa caacaatac aacgtagaa 
      acgaataac acgaggtcg gtcattaac agagcgaat 
      cccacgagc ggttctagt ctttttgtc aacgatcct 
      aacgcattc cccacatgg gacgactag
    mRNA sequence
    • >TC014779 RA
      ttcaatcaa caacacgtt ttcattaca atagtcgtg 
      ttaaaatgt gccaacctt gcgattatt cggccccca 
      atcacctca aacacctga aaatgtgaa atttccgtg 
      ggtttttct gtaggaatt tagacttca actagacat 
      gaaggtggg cgcttggac ggtcgatcg ggaggtcct 
      ccggagaga cggcttggt cctcttcag caaactcat 
      ccgaacaaa gaacgttga gggcctaca agaacaaag 
      cagaccgac ggggaccga tgaccgcaa acaaaagct 
      cacgaaatg tctgacgac tctggattt gacatcgct 
      gggagtggg aagctgtgt gggaactgg aatgtatgt 
      ggtggcagg aatggtggc aaaaaacta cgctggccc 
      tggcgtggt catcagttt catcattgc agcaatagc 
      cagcatatt ttcaggagc ctgctatgc agagttcgg 
      tgtcagggt gccacacac caccggctc cgcatacat 
      gtactcgta cgtcaccgt cggagaatt catcgcctt 
      cgtcattgg ctggaacat gatcttgga gtatttaat 
      tgggacatc ggcatgtgc ttgtgcact cagcgcctg 
      ttttaacgc attggcgaa tggggcaat tagggcggc 
      agtcgagaa gagcatctt tggcacaat gttcggccg 
      agccccgga ttttctggc tttcgtcat aaccatctt 
      gatgatgct gctcatggc agccggggt gaaaaaatc 
      gctcatatt caacaacgt cctcaatgc tattaatct 
      ggccgtttg ggtgtttac gatgactgc cggcctttt 
      ctacgtaaa ttccgacaa ctggaccaa acacgacgg 
      gtttctgcc gcacggatg gtcaggggt ctttactgg 
      agcagcgac ttgctttta cgccttcat cggcttcga 
      tatcattgc aacaacggg cgaagaagc caccaatcc 
      gaaaaaatc aatccctct cgccattat aacaagttt 
      gggaatcat cctgaccgc ctacgtcac cagttccat 
      gatgctgac attaatcat tcccttcaa cgaggtcga 
      cgagaattc ggccctggt cgaaatgtt cggccaagt 
      gggcgctta caaatgtaa atacatcgt ggcggtggg 
      tgccttggc cggcctcac cgtcagcat gttcgggtc 
      catgttccc catgcctcg gattattta cgcgatggc 
      tcaagacgg gttaatttt caaaatttt ttcgcaagt 
      gtggccgag cacgggaac cccggcttt agcaacgtt 
      cattagtgg gttggcagc aggtgtggc tgctctttt 
      tataaggtt agaaatcct cgtagaaat gatgtcgat 
      aggtaccct tttagctta tacgttagt atcgacatg 
      tgtcctaat attgaggta tcaacccca ctcgacaaa 
      tctagtaga gttgttacc tcaaagttt gagaactcc 
      attgagagg atctccaac gaaagaagg attgactca 
      aaatccgaa cggacaagc cttgtacgg gaaccaact 
      ccacccaga cgctttgaa acaagcgat tgacgctag 
      cttaggacc gccgattcc ttcaccgtc gccctcgcc 
      gctgcccac ccaaaccac gcagagggt catggtgag 
      gagggtcac caggagctc accagacag tgacgacac 
      ttacggagc agacgaaca agaagaaga gttctccat 
      gcgagacga ccagttcct agtttcaga ccggacgga 
      aaacaaatt ttacggaac tctgcacgg cgccggcag 
      cgcgaccag cacgggcgg ggttgtggg gtccaccgt 
      ggggcccag cctgggcta catcggccg ccggttaca 
      ggctgcgac ttatttatg ccctgcgat attcccatg 
      ggtggacac cggccctgc cactgaaga gtcggggat 
      gtttgtgat gaaaatggt gggaatatt gtatttatt 
      aatcataat tttcgatct gatgatggt ctgcgggat 
      gcacaacat gtcagggac tttaacatt ttttctttt 
      ccttttctt gttcgccat tattgcggt tttattagt 
      catatcgag gaagccaca aaatcgcaa cactttgat 
      gttcatgac accaggttt gcctttcgt tcctgccgt 
      cgcgatcac cgttaacat ctacctaat tttcaaact 
      gtcgatcct aactctagt cagattcac gatttggat 
      gactttggg attcatcat gtatttcta ctacgggat 
      taagaatag cattctcga ggaagggga aggagacca 
      aaatatcga actgactgt gaccgacca cgagaagcc 
      caaatacaa caacacaga tcagacgat tttcagcca 
      agaacaacc cacatatga ctgggatcc caagaccgt 
      ttgggacca tcagccctc atgggccca acccaccca 
      gcaatacca agtagaaca acaatacaa cgtagaaac 
      gaataacac gaggtcggt cattaacag agcgaatcc 
      cacgagcgg ttctagtct ttttgtcaa cgatcctaa 
      cgcattccc cacatggga cgactagtg agataggaa 
      gcgcgatct gttgttctg ttatcaaaa ttatggttg 
      ttatcgtat agttaaaaa ggactttaa ctggactga 
      tgttttcaa taattatgg tagagatag ttacgtttt 
      ttcgtatta ctgctagtc ataaattct aaaactggg 
      aggcatgag acaaggatt tagttgcac agtcgtgat 
      gcacaccct ctttccttg aattttata acggtgttt 
      gcaacattt tattaaatt gtaaatagc ggtgatatt 
      tgtaagtag actcattgt tttgcaatc ttttatcaa 
      gttgcctaa atatacagg gtgtcacga ttgaatctt 
      gctagttgt gaattaggt tgtgagtag ttaaggtct 
      tgcctttct ttttctatt agccaattg aatttcaaa 
      caccctgta tagattttc gaaacagat ctctaacca 
      tatcaattt tttcataca gttagtaat taattgatc 
      tctgtcaaa ttgtaacga gtgtccatg tttgtgact 
      tccaaattc tttctggtt taagttgtt gcgtcttcc 
      ctgtttgtg cactttttc gttttgttt ttagagaat 
      gcggaagtc agttacgcc atggaaacg gtaatctgg 
      tgataacac acggcacaa ttttactct agtatcaaa 
      aataattaa attagtgat tcgagcacc agttgatac 
      ttgggagga gaatttccc gagtatcgt tatcagtat 
      tataaattg cgatttttt gtggtttgt gtcgcttct 
      taggttgac ttgttgctt cgaatattg ttttgtatt 
      atacaagga actaactac ctgttgtaa atagttttg 
      agttgaagc aaaaactaa gtgttgcaa aatgttcta 
      aaaataccc aaaatgtat aaagaagca cgtacctaa 
      atgttgata gcttgttat tatcttatt tcagttgtc 
      ttaatcacg cctttgtaa atacacagg actgattaa 
      aatattaat taatatatt tcacatacg tcagtgcct 
      ggatttaag gaatgcatg aaagatatt tcggttgat 
      ttttcttat taaacgcat ttatcatga aatcgcaat 
      ttcctaagg ccacctctt ttcaattta agacaataa 
      atgtgcgtc tccacaatg ccactcact gcattttgc 
      ccgatttat ttgttaata acaataagt tcctctaac 
      tcttcctca caaacatat ccaggtttt gtggtaaat 
      gcgtcgcca atttcgtag gataactaa aaacgttat 
      gatgtctat tgatattaa gggtttcat tcttttgtg 
      tacttgtaa gtaaatcgt gaaaacatg tatgaacaa 
      cgaaacgtt attcctaat tgacttgaa aatgtttgt 
      acatgttgg gattacttg caatcgttt cgtaattat 
      ttttcagat tagggtttc tttcagtat tgtaaaggg 
      ttacatttg taatataag taattatag tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY