Status
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Gene information
General information
TC014763 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14791267..14795463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014763
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05375

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_06336

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014763 RA
      MYSAVRSSK SDASDNFTR QGSIRKVIS IEPKENLFG 
      RQNEDLNKL YKNFHQEYV ILAEYKMIQ SENIQGVYV 
      IPSRENPFI WFGVIFVRS GPYEDGVFR FNVVLDENF 
      PDSEHPKVV FLSEMFHPV VHPDTNEVH LLKAFPTWN 
      KADQHIWQV LKYIHWMFY NLSPTIAHS VNPEAADLF 
      ANNQEAFRN KVKEIVKTS QEKIYDEPP VEDKHYLVF 
      EPYNPEVHE QAKSTMLAQ NSEEGVKCG FSWVLPGSF 
      KPLGRPPSP DSESES
    CDS sequence
    • >TC014763 RA
      atgtatagc gccgttagg tcgtccaag tcagatgct 
      tcggataat tttacacga cagggctca atcagaaaa 
      gtcatatcc atcgaaccc aaggagaat ttatttggg 
      cgtcaaaat gaggacctc aataaatta tacaaaaac 
      tttcaccag gaatacgtc attttggcc gaatataaa 
      atgatccaa agcgagaac attcaaggt gtgtacgtg 
      attccgtcc agggagaac ccgtttatt tggtttggg 
      gttattttt gtccgaagt gggccttac gaagacggc 
      gtttttcgc tttaatgta gtccttgac gagaatttc 
      cccgatagt gaacaccct aaagtggtg tttttgtcc 
      gaaatgttc catcctgtt gtacaccct gacactaac 
      gaagtgcac cttttgaag gcgttcccc acgtggaac 
      aaagcagac caacatatc tggcaagtg ttgaagtac 
      atccactgg atgttttac aacttgagc cccactata 
      gcccatagt gtcaacccg gaagctgcc gatttattt 
      gccaacaac caagaggcc tttaggaac aaagttaaa 
      gaaattgta aaaacaagc caggagaaa atttacgat 
      gagccccca gttgaagac aaacactac ctagttttt 
      gagccctat aatccagag gtgcatgag caggccaaa 
      agtacgatg cttgcccag aacagcgag gaaggggtc 
      aagtgtggg ttctcttgg gtgcttcca gggagcttc 
      aaaccgtta gggcggccc ccttctcca gattcagaa 
      agcgagtca tga
    mRNA sequence
    • >TC014763 RA
      cggcccatg tacctttcg atgttggtc atgccagtt 
      gtaaatggt gtcaattgt caattttgc cttactttt 
      atctgatat tttataata ataggcctc aaaacatct 
      cttatcagc acattacag taactcaat aatgttcac 
      acacacatc gataatttc aggagagta aacaaccat 
      gtatagcgc cgttaggtc gtccaagtc agatgcttc 
      ggataattt tacacgaca gggctcaat cagaaaagt 
      catatccat cgaacccaa ggagaattt atttgggcg 
      tcaaaatga ggacctcaa taaattata caaaaactt 
      tcaccagga atacgtcat tttggccga atataaaat 
      gatccaaag cgagaacat tcaaggtgt gtacgtgat 
      tccgtccag ggagaaccc gtttatttg gtttggggt 
      tatttttgt ccgaagtgg gccttacga agacggcgt 
      ttttcgctt taatgtagt ccttgacga gaatttccc 
      cgatagtga acaccctaa agtggtgtt tttgtccga 
      aatgttcca tcctgttgt acaccctga cactaacga 
      agtgcacct tttgaaggc gttccccac gtggaacaa 
      agcagacca acatatctg gcaagtgtt gaagtacat 
      ccactggat gttttacaa cttgagccc cactatagc 
      ccatagtgt caacccgga agctgccga tttatttgc 
      caacaacca agaggcctt taggaacaa agttaaaga 
      aattgtaaa aacaagcca ggagaaaat ttacgatga 
      gcccccagt tgaagacaa acactacct agtttttga 
      gccctataa tccagaggt gcatgagca ggccaaaag 
      tacgatgct tgcccagaa cagcgagga aggggtcaa 
      gtgtgggtt ctcttgggt gcttccagg gagcttcaa 
      accgttagg gcggccccc ttctccaga ttcagaaag 
      cgagtcatg acggaggct tggagtgca attctacgc 
      ttattagta caagtttac gcaattttc acatgacac 
      caactgttc aacagtttg taaataggg agtgcaagg 
      cgcaataag aacaaagtt tttattaaa attgtttat 
      acaaatttt aactatcac gagtatttt aataaagca 
      aaagtatta cattattta ttttctttc taccagtta 
      tcgtcataa ttgaaattt gaattcaga aatgtgaca 
      acaaagctt ttaacaaaa tatttataa aaataaagt 
      attacgttt gcttgattc ttcccttgt accaattat 
      tatctagct cgtatgtgg ttcccccaa atgtgaaat 
      gagttggag ggttgccct cgccattat tgaacgtaa 
      aatgtttac taaattcaa tcgccagtt tggatctaa 
      tcaaaccaa cgccccctg cgcgttcag gtgctggat 
      ggtaaaccc tccccgtct ctggtatca tcacaactt 
      tatccggct caaacctaa atgtgagta aatccccag 
      catgattat gagccaaac gtgtctcct cattgtctt 
      tcaacatca catgagtga agacaattg gacagtcgt 
      cacatctaa taaaattgc gttcctttc cacaaatcg 
      acaaatacg gaaactcct tgtacctcc cactgttat 
      tcaatcgca gctgattga agaaaaaac gcaaaaatt 
      tcttctctt taaagcatg acgtaaagt tcttcattt 
      ttgaatcgt ctagaaaca gctgttttt gttagtgtt 
      gggactgtt ttgtctaat accattccc tggtggtca 
      atgtagtaa aaatactcc cttattttc ggctcaggc 
      tcttggcct tggacgtaa gacactttg cattggtga 
      cttgctgtg aagccccgt tttgcgcaa aaaaggacg 
      tttttgccc cttaacgtg ttacttaac gatactctc 
      atcgcgttt tgtttacaa caatgacgg actttaacc 
      gcaggggct tttattcga aaaattata cacagaaca 
      cagtggtgg actggtgac tgggcttat catgcggtt 
      gattactcg aaacgtcta aaatgggtt ttgtagatt 
      ttgaccaaa ttctattca cgtgacaca aaaattgcg 
      tttttcgtt tagaaagtg agtcaaaaa tataaacag 
      acaaaatgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY