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Gene information
General information
TC014726 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14124019..14126040
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014726
    iBeetle screen iB_06335

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014726 RA
      MMSIFKVNV SKKIMACAS VQVWQNDGL PSQICNKCS 
      AKLHISFQF KKQCEKSDA KLRQYLSNM TQENLQQPT 
      QQHVEHQQI QQVTQPQQI NKLDELDLN QQINQNNCV 
      YIECAPLLE IQQEQKFDP VFIPASQPQ QINYNVQSQ 
      NHLQLNSYN LQGVSQVQV YNGTYSMPL QPMQPPNIL 
      HNQVIAPQM QIQHQQQLP PLQPVMQQQ TVPQILEED 
      KDKGKKNSK VKRDNKVND TKQCPTCNK IFATSTKLS 
      RHMKTHSTD MPYKCKVCN KAFSHSGNY KIHLRMHTD 
      ERPFRCTVC DKGCRQAQD LEKHMRTHT GERPHKCNL 
      CTRAFSTSS NLIAHIRTH TGERPYVCC VCQKAFCQS 
      NELTKHMRT HTGEKSHIC DICHKGFNG SSTLIVHRR 
      SHTGERPYV CSVCNKGFA QSSCLAVHM KRHNGEKNF 
      ACNSCDKSF VTNADLKEH LLTHTGEKP FTCNICDKK 
      YSRANDLNR HTKTHTFKG IEES
    CDS sequence
    • >TC014726 RA
      atgatgtca atattcaaa gtaaacgtc tcaaaaaag 
      atcatggca tgtgcctca gtccaggtg tggcaaaac 
      gacggcctc ccgtcacaa atatgcaac aaatgctcg 
      gccaagctc cacatttcc ttccagttc aaaaaacag 
      tgcgaaaaa tcggacgca aaactgcgc caatatctt 
      tcaaacatg actcaagag aacctccaa cagccgaca 
      caacaacac gtggagcac caacagatc cagcaagtg 
      actcagccc caacaaatc aacaaactc gacgaacta 
      gacttgaac caacaaatc aaccaaaac aactgcgtg 
      tatattgag tgcgctccc cttttagaa atacaacag 
      gagcaaaaa ttcgatccg gtgtttatc ccagcatct 
      caaccccaa caaatcaac tacaatgtc cagtcacaa 
      aaccacctc cagttgaac agttacaac ctgcaagga 
      gtgagccaa gtccaagtc tacaacggc acctacagc 
      atgcccctg caacccatg cagcccccc aacatcctc 
      cacaaccaa gtcatcgcc ccacaaatg caaatacaa 
      caccaacaa caattacca ccactccag ccagttatg 
      caacaacag acagttccg caaattctg gaagaagat 
      aaagacaaa gggaagaaa aatagcaag gtgaaaaga 
      gacaataaa gtgaatgac actaaacag tgtcccact 
      tgtaataaa atcttcgcc acttcgacc aaattaagc 
      aggcatatg aagacgcac tcaactgat atgccgtac 
      aaatgcaaa gtttgcaat aaggcgttt tcacacagc 
      gggaattac aaaatccac ctaagaatg cacacagac 
      gagaggccg ttcaggtgc acagtttgc gataaaggc 
      tgccggcaa gcgcaggac ttggaaaag catatgagg 
      actcacaca ggagaaagg ccccacaag tgcaacctc 
      tgcactcgc gctttctcc acaagctcg aatttaata 
      gcccacata aggacacac acaggagag cgcccttac 
      gtctgctgc gtgtgccaa aaggccttt tgccagtcg 
      aacgaacta acaaaacac atgaggact cacacggga 
      gagaagtca cacatttgt gacatttgc cataaaggt 
      tttaatggt tccagtacc ttgattgtg cacagaagg 
      tcgcataca ggggaacgt ccttatgtt tgctcagtc 
      tgtaataag ggtttcgcc caatcgagt tgcttggca 
      gtgcatatg aaaagacac aacggtgaa aaaaatttc 
      gcgtgtaac tcgtgtgat aagtcattt gtgacaaat 
      gccgatctt aaggaacat ttgttgact catacgggc 
      gagaaaccc tttacttgc aatatttgt gataagaaa 
      tactcaaga gccaatgat ctcaacaga cataccaag 
      acacatact tttaagggc attgaggag agctag
    mRNA sequence
    • >TC014726 RA
      tcccactga cagttcgtt gtgtttaca agctttagg 
      ttaacaaaa acacaacat cgatgatat aacagccaa 
      aaaaacagc acatagcaa aaaatggaa ttagacttc 
      tcaaaaata tgccgtgtg tgtctaaac gaaggcata 
      atgatgtca atattcaaa gtaaacgtc tcaaaaaag 
      atcatggca tgtgcctca gtccaggtg tggcaaaac 
      gacggcctc ccgtcacaa atatgcaac aaatgctcg 
      gccaagctc cacatttcc ttccagttc aaaaaacag 
      tgcgaaaaa tcggacgca aaactgcgc caatatctt 
      tcaaacatg actcaagag aacctccaa cagccgaca 
      caacaacac gtggagcac caacagatc cagcaagtg 
      actcagccc caacaaatc aacaaactc gacgaacta 
      gacttgaac caacaaatc aaccaaaac aactgcgtg 
      tatattgag tgcgctccc cttttagaa atacaacag 
      gagcaaaaa ttcgatccg gtgtttatc ccagcatct 
      caaccccaa caaatcaac tacaatgtc cagtcacaa 
      aaccacctc cagttgaac agttacaac ctgcaagga 
      gtgagccaa gtccaagtc tacaacggc acctacagc 
      atgcccctg caacccatg cagcccccc aacatcctc 
      cacaaccaa gtcatcgcc ccacaaatg caaatacaa 
      caccaacaa caattacca ccactccag ccagttatg 
      caacaacag acagttccg caaattctg gaagaagat 
      aaagacaaa gggaagaaa aatagcaag gtgaaaaga 
      gacaataaa gtgaatgac actaaacag tgtcccact 
      tgtaataaa atcttcgcc acttcgacc aaattaagc 
      aggcatatg aagacgcac tcaactgat atgccgtac 
      aaatgcaaa gtttgcaat aaggcgttt tcacacagc 
      gggaattac aaaatccac ctaagaatg cacacagac 
      gagaggccg ttcaggtgc acagtttgc gataaaggc 
      tgccggcaa gcgcaggac ttggaaaag catatgagg 
      actcacaca ggagaaagg ccccacaag tgcaacctc 
      tgcactcgc gctttctcc acaagctcg aatttaata 
      gcccacata aggacacac acaggagag cgcccttac 
      gtctgctgc gtgtgccaa aaggccttt tgccagtcg 
      aacgaacta acaaaacac atgaggact cacacggga 
      gagaagtca cacatttgt gacatttgc cataaaggt 
      tttaatggt tccagtacc ttgattgtg cacagaagg 
      tcgcataca ggggaacgt ccttatgtt tgctcagtc 
      tgtaataag ggtttcgcc caatcgagt tgcttggca 
      gtgcatatg aaaagacac aacggtgaa aaaaatttc 
      gcgtgtaac tcgtgtgat aagtcattt gtgacaaat 
      gccgatctt aaggaacat ttgttgact catacgggc 
      gagaaaccc tttacttgc aatatttgt gataagaaa 
      tactcaaga gccaatgat ctcaacaga cataccaag 
      acacatact tttaagggc attgaggag agctagccc 
      ttgtagctc gatttatca gtatttgat tgaagtatt 
      agaacgttt ggccaaaaa tttacgaat agtatttat 
      gtagatatt gatggagta tagtgtact cgttattta 
      tgataataa ataaataaa tccacgata c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY