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Gene information
General information
TC033242
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014721
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13985337..13994109
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033242
    iBeetle screen iB_06334

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection



    Pupal morphology
    urogomphi potentially structure multiplied - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033242 RA
      MMSRHGKKH NRRHHYIPF GRNRRGSAD SNYSQPSSD 
      LSLDEEKET LRREKERQA LSQLEKART KPVAFAVRT 
      NVAYDGSLD DDSPVHGYA ISFEVRDFL HIKEKYDNN 
      WWIGRLVKE GCDIGFIPS PVKLENLRL QQAQSRTTK 
      LYSSKTSSS SNIGAAGND VSRGSTPPT PGDESDSVG 
      NARSGKPLT TPPAKEKKK PFFKKQEAT APYDVVPSM 
      RPVVLVGPS LKGYEVTDM MQKALFDFL KHRFEGRII 
      ITRVMADIS LAKRSLLNN PSKRAIMER STTRSTCLS 
      EVQAEIERI FELATTLQL VVLDCDTIN HPSQLAKTS 
      LAPCIVYLK ITSPKVLQR LIKSRGKSQ ARHLNVQMV 
      ASEKLAQCP PEMFDVILD ENQLEDACE HIAEYLEAY 
      WRATHPEVN VSNVPRPIG SSPQASPSG DQGRPTLPV 
      PTFHTSHTR SRQRLERER EYEEYAERD SYTHANTHG 
      MPHHGGDDY RIPARDHRA AREEEYPTH RGSSRRALN 
      AI
    • >TC033242 RB
      MIGYNNFNN QGYNKLFTQ GSADSNYSQ PSSDLSLDE 
      EKETLRREK ERQALSQLE KARTKPVAF AVRTNVAYD 
      GSLDDDSPV HGYAISFEV RDFLHIKEK YDNNWWIGR 
      LVKEGCDIG FIPSPVKLE NLRLQQAQS RTTKLYSSK 
      TSSSSNIGA AGNDVSRGS TPPTPGDES DSVGNARSG 
      KPLTTPPAK EKKKPFFKK QEATAPYDV VPSMRPVVL 
      VGPSLKGYE VTDMMQKAL FDFLKHRFE GRIIITRVM 
      ADISLAKRS LLNNPSKRA IMERSTTRS TCLSEVQAE 
      IERIFELAT TLQLVVLDC DTINHPSQL AKTSLAPCI 
      VYLKITSPK VLQRLIKSR GKSQARHLN VQMVASEKL 
      AQCPPEMFD VILDENQLE DACEHIAEY LEAYWRATH 
      PEVNVSNVP RPIGSSPQA SPSGDQGRP TLPVPTFHT 
      SHTRSRQRL EREREYEEY AERDSYTHA NTHGMPHHG 
      GDDYRIPAR DHRAAREEE YPTHRGSSR RALNAI
    CDS sequence
    • >TC033242 RA
      atgatgtcc agacacggg aaaaagcac aatcggcgt 
      caccactac ataccattc ggacgtaat cgccggggc 
      tcagctgat tctaactac tctcagcct tcttcggat 
      ctgtcgctg gacgaggag aaagagacg ttgcgacgg 
      gagaaggaa cgtcaagcg cttagtcag ttggaaaaa 
      gcaagaacg aaaccggta gcatttgct gttagaacc 
      aacgtagcg tatgacggt tcattggac gacgattcg 
      cccgtccat ggatacgca atttctttc gaagttagg 
      gatttttta cacattaag gaaaagtac gacaacaat 
      tggtggata ggtcgtttg gtgaaggaa gggtgcgat 
      attgggttt attccttct ccggtaaag ctcgagaat 
      ctccgcttg cagcaagca cagtcgagg actacaaaa 
      ctctactca agtaaaaca agctccagc tctaatata 
      ggggccgct ggtaacgat gtttccaga ggtagcact 
      cctcccacg cctggagac gagtctgat tcagttggt 
      aacgctcga tcaggcaag cctctcaca acgcctccg 
      gcaaaagag aagaagaaa ccctttttt aagaagcag 
      gaagcaact gctccttac gacgtagtt ccctccatg 
      aggccagta gttctagta ggtccttcg ttgaaagga 
      tatgaagtt acagatatg atgcaaaaa gccttattt 
      gatttttta aaacatagg tttgaagga agaataatt 
      atcacgcga gtaatggca gatatatct ttggcaaag 
      cgttcactt ttaaataat ccttcgaaa cgggcgata 
      atggaacgt tccacaact cgctcaact tgtttgtca 
      gaggtccaa gcagaaatc gaacgaata ttcgagcta 
      gctacaact ttacaactt gtggtgctg gattgtgat 
      actataaac catccttcg caacttgcc aaaacttca 
      ttagctcca tgtattgtc tatcttaaa ataactagt 
      cctaaggtt ttgcaaagg ctaataaaa tctaggggt 
      aaaagtcag gcgagacat ctgaatgta caaatggtc 
      gcgtcagaa aaactagct caatgtcct ccggaaatg 
      ttcgacgtg atcttggac gagaaccag ctggaggat 
      gcgtgtgaa cacatagcg gaatacttg gaggcatat 
      tggagagca actcatcct gaagtgaac gtttccaac 
      gttcctcga cccattggt agttcaccg caagcttct 
      ccgagtggg gaccaaggc aggcccaca ctaccagtt 
      cccacattt catacgtcg cacacgaga agtagacag 
      aggttagag cgagagcgt gagtatgaa gagtacgcc 
      gaacgggat agttacact catgcaaac acccatgga 
      atgccccac catggaggc gacgattat cggatacca 
      gcgagagac cacagggcg gctcgggag gaagaatac 
      ccgacacat aggggatct tcaaggaga gcgctgaac 
      gccatatag
    • >TC033242 RB
      atgatagga tacaacaat ttcaacaat cagggatac 
      aataaactg tttactcag ggctcagct gattctaac 
      tactctcag ccttcttcg gatctgtcg ctggacgag 
      gagaaagag acgttgcga cgggagaag gaacgtcaa 
      gcgcttagt cagttggaa aaagcaaga acgaaaccg 
      gtagcattt gctgttaga accaacgta gcgtatgac 
      ggttcattg gacgacgat tcgcccgtc catggatac 
      gcaatttct ttcgaagtt agggatttt ttacacatt 
      aaggaaaag tacgacaac aattggtgg ataggtcgt 
      ttggtgaag gaagggtgc gatattggg tttattcct 
      tctccggta aagctcgag aatctccgc ttgcagcaa 
      gcacagtcg aggactaca aaactctac tcaagtaaa 
      acaagctcc agctctaat ataggggcc gctggtaac 
      gatgtttcc agaggtagc actcctccc acgcctgga 
      gacgagtct gattcagtt ggtaacgct cgatcaggc 
      aagcctctc acaacgcct ccggcaaaa gagaagaag 
      aaacccttt tttaagaag caggaagca actgctcct 
      tacgacgta gttccctcc atgaggcca gtagttcta 
      gtaggtcct tcgttgaaa ggatatgaa gttacagat 
      atgatgcaa aaagcctta tttgatttt ttaaaacat 
      aggtttgaa ggaagaata attatcacg cgagtaatg 
      gcagatata tctttggca aagcgttca cttttaaat 
      aatccttcg aaacgggcg ataatggaa cgttccaca 
      actcgctca acttgtttg tcagaggtc caagcagaa 
      atcgaacga atattcgag ctagctaca actttacaa 
      cttgtggtg ctggattgt gatactata aaccatcct 
      tcgcaactt gccaaaact tcattagct ccatgtatt 
      gtctatctt aaaataact agtcctaag gttttgcaa 
      aggctaata aaatctagg ggtaaaagt caggcgaga 
      catctgaat gtacaaatg gtcgcgtca gaaaaacta 
      gctcaatgt cctccggaa atgttcgac gtgatcttg 
      gacgagaac cagctggag gatgcgtgt gaacacata 
      gcggaatac ttggaggca tattggaga gcaactcat 
      cctgaagtg aacgtttcc aacgttcct cgacccatt 
      ggtagttca ccgcaagct tctccgagt ggggaccaa 
      ggcaggccc acactacca gttcccaca tttcatacg 
      tcgcacacg agaagtaga cagaggtta gagcgagag 
      cgtgagtat gaagagtac gccgaacgg gatagttac 
      actcatgca aacacccat ggaatgccc caccatgga 
      ggcgacgat tatcggata ccagcgaga gaccacagg 
      gcggctcgg gaggaagaa tacccgaca catagggga 
      tcttcaagg agagcgctg aacgccata tag
    mRNA sequence
    • >TC033242 RA
      attcttaaa ataacaaaa ggcaaatta ctgtgattt 
      agacttgtt ttagtgata gtgttactt tagtgctgt 
      gatatttac aatgatgtc cagacacgg gaaaaagca 
      caatcggcg tcaccacta cataccatt cggacgtaa 
      tcgccgggg ctcagctga ttctaacta ctctcagcc 
      ttcttcgga tctgtcgct ggacgagga gaaagagac 
      gttgcgacg ggagaagga acgtcaagc gcttagtca 
      gttggaaaa agcaagaac gaaaccggt agcatttgc 
      tgttagaac caacgtagc gtatgacgg ttcattgga 
      cgacgattc gcccgtcca tggatacgc aatttcttt 
      cgaagttag ggatttttt acacattaa ggaaaagta 
      cgacaacaa ttggtggat aggtcgttt ggtgaagga 
      agggtgcga tattgggtt tattccttc tccggtaaa 
      gctcgagaa tctccgctt gcagcaagc acagtcgag 
      gactacaaa actctactc aagtaaaac aagctccag 
      ctctaatat aggggccgc tggtaacga tgtttccag 
      aggtagcac tcctcccac gcctggaga cgagtctga 
      ttcagttgg taacgctcg atcaggcaa gcctctcac 
      aacgcctcc ggcaaaaga gaagaagaa acccttttt 
      taagaagca ggaagcaac tgctcctta cgacgtagt 
      tccctccat gaggccagt agttctagt aggtccttc 
      gttgaaagg atatgaagt tacagatat gatgcaaaa 
      agccttatt tgatttttt aaaacatag gtttgaagg 
      aagaataat tatcacgcg agtaatggc agatatatc 
      tttggcaaa gcgttcact tttaaataa tccttcgaa 
      acgggcgat aatggaacg ttccacaac tcgctcaac 
      ttgtttgtc agaggtcca agcagaaat cgaacgaat 
      attcgagct agctacaac tttacaact tgtggtgct 
      ggattgtga tactataaa ccatccttc gcaacttgc 
      caaaacttc attagctcc atgtattgt ctatcttaa 
      aataactag tcctaaggt tttgcaaag gctaataaa 
      atctagggg taaaagtca ggcgagaca tctgaatgt 
      acaaatggt cgcgtcaga aaaactagc tcaatgtcc 
      tccggaaat gttcgacgt gatcttgga cgagaacca 
      gctggagga tgcgtgtga acacatagc ggaatactt 
      ggaggcata ttggagagc aactcatcc tgaagtgaa 
      cgtttccaa cgttcctcg acccattgg tagttcacc 
      gcaagcttc tccgagtgg ggaccaagg caggcccac 
      actaccagt tcccacatt tcatacgtc gcacacgag 
      aagtagaca gaggttaga gcgagagcg tgagtatga 
      agagtacgc cgaacggga tagttacac tcatgcaaa 
      cacccatgg aatgcccca ccatggagg cgacgatta 
      tcggatacc agcgagaga ccacagggc ggctcggga 
      ggaagaata cccgacaca taggggatc ttcaaggag 
      agcgctgaa cgccatata gtggaggct ttctacgga 
      acagccaat tgaagagga aatgcatga tgacctttc 
      tcccagagc ggaaactta ttacaaacc tcagattcc 
      gaaagagta cagagacga gcggctgcg ccacgacga 
      ggaccgaag gcgagtgga ggaggaacg aagatacgg 
      cgagaggag cagataccc agaagatca cagatacga 
      agatcggag atacgtcga agacaggaa ggcgatcga 
      gaagagtaa gcaagaggg caggaacta ccggaagga 
      gctactgga gaggtttgc tgatatgga tttcgaaga 
      gcatcatga cttccgaac gcatcaaag atactacga 
      ataaaccat cttagataa ataaagatt ttggcctaa 
      attactgaa attgtgtac aaatgcatt cagagtatc 
      tttgacgca ttcttcaat gggatgttc cgtaattgt 
      gattaattc ttactataa acattaaaa attttgacc 
      agtttggtg ccatatttg agtaatagc tcaattttt 
      aatcaatct attattgac atagaatat tcaagcttt 
      aaactgtag ctatactta ggattttta aatttattt 
      ataaggaat ggtatattg ttgaaagca gtataaatt 
      tatgtattt tatatattg taaaatagc ttaaaaaag 
      aactgagat attatactt ttaacggaa agtgtactc 
      atctgtact gggttatcg tttcgttgc gttctgttt 
      catagtttc atgatacct acgtgatat taacgtcaa 
      gtatttgac tgaatctag gaaatctag tcaagttga 
      agtttgtgc actgctgtt agaaatgtt atgagaagt 
      agataaaat gcatgaggc atcgaggct tttgaagaa 
      tagttacga attcatgat aaaccgtat aagatatcg 
      taatatttt ttaataaat tactagacc tttttccgt 
      ggtttgtca cctacccga cttggttct cttacaaat 
      aatgttgat gacaatcaa agtgtgttt tctaattta 
      gcgttagtt tagatcgat ttgacacca caaatattc 
      cgagtttag ttagaatct gattagtta gaagatccc 
      gacgacgta caaaatttt cgttaagtg taggtgata 
      aaaccaccg aaaataaat aggcttaca aactgtaca 
      tttcgaaac tgcatgtgc cactatagt ggtaaatat 
      cactgacca atttcacta cacaacaaa atatttgta 
      taggagtag atctgtgaa atcctttta ggttttgca 
      cttggccct aacaaagtt acaagcgac aattatcat 
      ttagttagt gtgttgacg ggttagagg aattattgt 
      gactttgtt agctgcagt ttcttttat tagtcaact 
      tagaacccc ggttttctt tcaaatatt ttattgtat 
      ctagagtta aatacttat aacaaggtt tcatatgct 
      gttgatatt gaatgagtc tattgatat ttcaaaata 
      taagtaatt aatatttaa ataaagagc taaaatttg 
      atacattgt aaatagcta ttaaatatt gaaaaatct 
      atactactg agaaattgt tttgttgat ttgtatcga 
      atagctaga ttgggtttt gttgtttgt gtcattgat 
      tttgtatat aatcttagt tggatacgt ttatctaaa 
      tgccttgct gttatacat gatttcttt ttatataag 
      tatcaattc aaatcatat gaatgtaag aatatggtg 
      aaaggcact attgatgtg cattttttc taaataaaa 
      tacctatat taaa
    • >TC033242 RB
      attcttaaa ataacaaaa ggcaaatta ctgtgattt 
      agacttgtt ttagtgata gtgttactt tagtgctgt 
      gatatttac aatgatgtc cagacacgg gaaaaagca 
      caatcggcg tcaccacta cataccatt cggacgtaa 
      tcgccggtc aaaagctca aagctctac cctatttga 
      aacgttgag cttaaaatg cggcgccga ctatactca 
      acctagcgg cgttcaccc gcattacac tacgtatat 
      tcttgagca aaacattac ttttccatt cagaaagtt 
      gttcgtgtc ataaatatg gttaatgtg gcgttcgcc 
      ggtgatttg tgagatttg gtttgcgga gatccatct 
      cgaagcgcg ttctgctaa tttatgaat caccgatta 
      atcgcttaa taggggtac gtttttgcg attttcggg 
      acgttcatt gcgctataa tcctgcctt tattggatg 
      gtgcacacg gatgtgtca ttgaaatgt cgcaatatg 
      ataggatac aacaatttc aacaatcag ggatacaat 
      aaactgttt actcagggc tcagctgat tctaactac 
      tctcagcct tcttcggat ctgtcgctg gacgaggag 
      aaagagacg ttgcgacgg gagaaggaa cgtcaagcg 
      cttagtcag ttggaaaaa gcaagaacg aaaccggta 
      gcatttgct gttagaacc aacgtagcg tatgacggt 
      tcattggac gacgattcg cccgtccat ggatacgca 
      atttctttc gaagttagg gatttttta cacattaag 
      gaaaagtac gacaacaat tggtggata ggtcgtttg 
      gtgaaggaa gggtgcgat attgggttt attccttct 
      ccggtaaag ctcgagaat ctccgcttg cagcaagca 
      cagtcgagg actacaaaa ctctactca agtaaaaca 
      agctccagc tctaatata ggggccgct ggtaacgat 
      gtttccaga ggtagcact cctcccacg cctggagac 
      gagtctgat tcagttggt aacgctcga tcaggcaag 
      cctctcaca acgcctccg gcaaaagag aagaagaaa 
      ccctttttt aagaagcag gaagcaact gctccttac 
      gacgtagtt ccctccatg aggccagta gttctagta 
      ggtccttcg ttgaaagga tatgaagtt acagatatg 
      atgcaaaaa gccttattt gatttttta aaacatagg 
      tttgaagga agaataatt atcacgcga gtaatggca 
      gatatatct ttggcaaag cgttcactt ttaaataat 
      ccttcgaaa cgggcgata atggaacgt tccacaact 
      cgctcaact tgtttgtca gaggtccaa gcagaaatc 
      gaacgaata ttcgagcta gctacaact ttacaactt 
      gtggtgctg gattgtgat actataaac catccttcg 
      caacttgcc aaaacttca ttagctcca tgtattgtc 
      tatcttaaa ataactagt cctaaggtt ttgcaaagg 
      ctaataaaa tctaggggt aaaagtcag gcgagacat 
      ctgaatgta caaatggtc gcgtcagaa aaactagct 
      caatgtcct ccggaaatg ttcgacgtg atcttggac 
      gagaaccag ctggaggat gcgtgtgaa cacatagcg 
      gaatacttg gaggcatat tggagagca actcatcct 
      gaagtgaac gtttccaac gttcctcga cccattggt 
      agttcaccg caagcttct ccgagtggg gaccaaggc 
      aggcccaca ctaccagtt cccacattt catacgtcg 
      cacacgaga agtagacag aggttagag cgagagcgt 
      gagtatgaa gagtacgcc gaacgggat agttacact 
      catgcaaac acccatgga atgccccac catggaggc 
      gacgattat cggatacca gcgagagac cacagggcg 
      gctcgggag gaagaatac ccgacacat aggggatct 
      tcaaggaga gcgctgaac gccatatag tggaggctt 
      tctacggaa cagccaatt gaagaggaa atgcatgat 
      gacctttct cccagagcg gaaacttat tacaaacct 
      cagattccg aaagagtac agagacgag cggctgcgc 
      cacgacgag gaccgaagg cgagtggag gaggaacga 
      agatacggc gagaggagc agataccca gaagatcac 
      agatacgaa gatcggaga tacgtcgaa gacaggaag 
      gcgatcgag aagagtaag caagagggc aggaactac 
      cggaaggag ctactggag aggtttgct gatatggat 
      ttcgaagag catcatgac ttccgaacg catcaaaga 
      tactacgaa taaaccatc ttagataaa taaagattt 
      tggcctaaa ttactgaaa ttgtgtaca aatgcattc 
      agagtatct ttgacgcat tcttcaatg ggatgttcc 
      gtaattgtg attaattct tactataaa cattaaaaa 
      ttttgacca gtttggtgc catatttga gtaatagct 
      caattttta atcaatcta ttattgaca tagaatatt 
      caagcttta aactgtagc tatacttag gatttttaa 
      atttattta taaggaatg gtatattgt tgaaagcag 
      tataaattt atgtatttt atatattgt aaaatagct 
      taaaaaaga actgagata ttatacttt taacggaaa 
      gtgtactca tctgtactg ggttatcgt ttcgttgcg 
      ttctgtttc atagtttca tgataccta cgtgatatt 
      aacgtcaag tatttgact gaatctagg aaatctagt 
      caagttgaa gtttgtgca ctgctgtta gaaatgtta 
      tgagaagta gataaaatg catgaggca tcgaggctt 
      ttgaagaat agttacgaa ttcatgata aaccgtata 
      agatatcgt aatattttt taataaatt actagacct 
      ttttccgtg gtttgtcac ctacccgac ttggttctc 
      ttacaaata atgttgatg acaatcaaa gtgtgtttt 
      ctaatttag cgttagttt agatcgatt tgacaccac 
      aaatattcc gagtttagt tagaatctg attagttag 
      aagatcccg acgacgtac aaaattttc gttaagtgt 
      aggtgataa aaccaccga aaataaata ggcttacaa 
      actgtacat ttcgaaact gcatgtgcc actatagtg 
      gtaaatatc actgaccaa tttcactac acaacaaaa 
      tatttgtat aggagtaga tctgtgaaa tccttttag 
      gttttgcac ttggcccta acaaagtta caagcgaca 
      attatcatt tagttagtg tgttgacgg gttagagga 
      attattgtg actttgtta gctgcagtt tcttttatt 
      agtcaactt agaaccccg gttttcttt caaatattt 
      tattgtatc tagagttaa atacttata acaaggttt 
      catatgctg ttgatattg aatgagtct attgatatt 
      tcaaaatat aagtaatta atatttaaa taaagagct 
      aaaatttga tacattgta aatagctat taaatattg 
      aaaaatcta tactactga gaaattgtt ttgttgatt 
      tgtatcgaa tagctagat tgggttttg ttgtttgtg 
      tcattgatt ttgtatata atcttagtt ggatacgtt 
      tatctaaat gccttgctg ttatacatg atttctttt 
      tatataagt atcaattca aatcatatg aatgtaaga 
      atatggtga aaggcacta ttgatgtgc attttttct 
      aaataaaat acctatatt aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY