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Gene information
General information
TC014550 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11519481..11520658
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014550
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06333

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands mostly content not present - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014550 RA
      MDTVQDSPE QQNFFKRLV KFISYPFVA VGRKLILWL 
      HFTELLFIR LYNIYALIC QLFFFVCCA VVYHESTEL 
      EKHEEHVLI ALKTLLFYS VFTYFTTKT RDILTTNRT 
      SLFRNFSMM EGVCRIIIE WAKAIIVVF CLREQGIYF 
      RPSIPYAAT TFTYYLCTE KIFTEILQK VIKYCEFQI 
      FEDLDHLYV PLALNVYTM TMSFTADLY LILTTEFVT 
      FSLCCAYFT IYLRLKDSY YNFWKLLVL EKQTFNSFR 
      DASQQDLDD YDDICAVCL NKMSKAKIT PCNHFFHPY 
      CLKECLKNS FLCPLCKVN F
    CDS sequence
    • >TC014550 RA
      atggataca gttcaggat tcccctgaa caacaaaat 
      ttcttcaag cgtcttgtc aagttcatc tcataccca 
      tttgtggcg gttggacgt aaactaatt ctctggttg 
      cacttcacg gaactttta ttcataaga ctttacaat 
      atttacgcc ctcatatgt cagttgttc ttttttgtc 
      tgctgtgca gtggtgtac cacgaatct acagaactc 
      gaaaagcat gaagaacac gttcttatt gcactgaaa 
      actttactg ttttactcc gtctttacg tatttcacg 
      acaaagacc agagacatt ttaacaacg aatcgaacg 
      tctcttttc cgtaatttt agtatgatg gaaggggtt 
      tgcaggatt ataattgaa tgggcgaaa gccattatt 
      gtggtgttt tgtctccga gaacaaggg atttatttc 
      agacccagc atcccctac gctgcaacc accttcact 
      tattatctc tgcaccgaa aaaatcttt accgagatt 
      ttgcaaaaa gtgatcaag tattgcgag tttcaaata 
      ttcgaggat ttagatcac ttatacgtg ccgttggct 
      ttaaatgtt tacaccatg acgatgagc tttacagcc 
      gatttatat ttgatttta acgaccgaa tttgtaacg 
      ttttcactg tgttgtgcc tactttaca atatatctt 
      agactgaaa gattcctat tataatttt tggaagctt 
      ctcgtgtta gaaaagcag acttttaac agttttcgc 
      gacgcaagt cagcaagac ttagatgac tacgacgat 
      atttgcgcc gtttgtttg aataagatg agcaaggcg 
      aaaatcaca ccttgcaac cacttcttc cacccttat 
      tgcctcaaa gagtgtctt aaaaactca ttcttgtgt 
      cctctgtgc aaagtgaac ttttaa
    mRNA sequence
    • >TC014550 RA
      ctagtgctg atgacataa tgactttca gtcactgtc 
      agaatcaac atgtagcct caaaatttt cccactgaa 
      tcaccagaa tggatacag ttcaggatt cccctgaac 
      aacaaaatt tcttcaagc gtcttgtca agttcatct 
      catacccat ttgtggcgg ttggacgta aactaattc 
      tctggttgc acttcacgg aacttttat tcataagac 
      tttacaata tttacgccc tcatatgtc agttgttct 
      tttttgtct gctgtgcag tggtgtacc acgaatcta 
      cagaactcg aaaagcatg aagaacacg ttcttattg 
      cactgaaaa ctttactgt tttactccg tctttacgt 
      atttcacga caaagacca gagacattt taacaacga 
      atcgaacgt ctcttttcc gtaatttta gtatgatgg 
      aaggggttt gcaggatta taattgaat gggcgaaag 
      ccattattg tggtgtttt gtctccgag aacaaggga 
      tttatttca gacccagca tcccctacg ctgcaacca 
      ccttcactt attatctct gcaccgaaa aaatcttta 
      ccgagattt tgcaaaaag tgatcaagt attgcgagt 
      ttcaaatat tcgaggatt tagatcact tatacgtgc 
      cgttggctt taaatgttt acaccatga cgatgagct 
      ttacagccg atttatatt tgattttaa cgaccgaat 
      ttgtaacgt tttcactgt gttgtgcct actttacaa 
      tatatctta gactgaaag attcctatt ataattttt 
      ggaagcttc tcgtgttag aaaagcaga cttttaaca 
      gttttcgcg acgcaagtc agcaagact tagatgact 
      acgacgata tttgcgccg tttgtttga ataagatga 
      gcaaggcga aaatcacac cttgcaacc acttcttcc 
      acccttatt gcctcaaag agtgtctta aaaactcat 
      tcttgtgtc ctctgtgca aagtgaact tttaaataa 
      aactacaat caaaagctt atcgtttta ttactgggt 
      cgaccgatt aattagaga gaaagaaaa ctaaaatta 
      atga
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