| General information | |||
| TC014283 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 8370955..8391530 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014283 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06327 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature dorsal not fused - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC014283 RA
MSPKTFALT AIGLTIILC VYYANYTTD IRTNQTQEN SKESDELPQ PQRIFELLQ RSKFATNKE FEVCPKLYP AEADISTVD VFKDFEFQP TWMKSKEYW DKSFEDRFE RQKMDAERP PLKVIIVPH SHNDPGWLK TFENYFHYV SRQIMNHMV AKLQQYKNL TFIWSEVAF LNAWWEEAH PSKQRALRN LVHSGRLEI ATGGWVMTD EANAHVYAM VDQLIEGHQ WVWSNLGVK PVSGWSVDP FGHGSTQPY ILASSGIKG TVIQRIHYA WKQWLALKQ YGDFRWVPT WSPASPSAA LLTHNQPFD IYSIKHSCG PHPYICLNF DFRKVIGEY TEYSIKAQA ITDKNIKEK SELLLEQYA RTGSLFPHN VVLMPLGDD FRYNVAEEW DQQYTNYIK LIDYINANK DTYKAEVGF GTPKDYFRE IMKRYDQYP TLKGDFFVY SDIFSEGRP AYWSGYFTT RPFMKILDR ELENSLRSA EILYTIALN NARQNKLLP YVKILERDF EKLVRARRN LGLFQHHDA ITGTSKSFV MRDYGLKLF EGIRDTVSI QQNSLQSLL LPDIPLKPG ENAILSDLE RESFEKLPK KTPIGLGPR QVRKIVLYN SLAQPREQL IQVRTNTSS VKIVDNEGN EVVSQVNPV WDTSESNTK LWIAADEFE LVFVAKLKE LSLTTYSLV YEENETRLA TIYCNKCHR KASIITNTD STIGKQNDV LKSKFAIKN IPTGDIQLE NHKFKILFN GNTGFMKTV TRKHSPKIM QCGLQFAAY RSAQFHSGA YLFMPDPNE RDIEKDVLQ QYKDQMSII ITSGPVSSE ITVIYGAFL VHSVAIYHV ETPLADAIY TESTVDFEN PPKNRETEL FMRIISDVQ NGEVPEFYT DQNGFQMLK RVKVEKIGI EGNYFPVTT MAYIQDDNV RLSLLTDHA QGASGWQPG FLEVMLDRR TLYDDSRGM GEGLVDNRK TVNRFWILI EDVVKEKGE KGHSKRFEN SDDTFEKGD VVRSFNVPT DPVEAGKQE GFPRASLFA NRMSNGLNY PVGIFILDH EHQEVKSLD LVRREFPCD THLLTLRTQ PDSVYSQFP SSSALLVLH RQGYDCAVS RNFSCSLSK FDSGFEHVK VKEIQAKTL TGIEDLPDN VKHLSDIYL EPMTLKTFN MTFV
- >TC014283 RA
atgtctcca aagaccttc gccttgacg gccataggc ctcacaata atcctgtgc gtgtactac gccaattac acgacagac attcgcaca aatcaaacg caagaaaat tcaaaagaa agcgacgaa ctgccccaa cctcagcgc atcttcgag ctcctccaa agatcaaaa ttcgcaact aataaagag ttcgaagtg tgtccgaag ctgtatcct gccgaggcc gacataagc acagtggat gtgtttaag gattttgag tttcagccg acttggatg aagagcaaa gagtactgg gacaagtcc tttgaagat cgatttgaa cgccaaaaa atggacgct gaaaggcca ccactgaag gtgataatc gttcctcac tcccataac gaccccgga tggctgaaa accttcgag aattacttc cactacgtc tcccgccag ataatgaac cacatggtg gcgaaactc cagcagtac aaaaaccta acattcatt tggtcggaa gtggccttt ctgaacgcc tggtgggag gaggcccat cccagtaaa cagcgagct ttgcgaaac ttggtccac tcagggcgc ctcgaaatc gccaccggt ggctgggtg atgaccgac gaagccaac gctcacgtc tacgccatg gtggaccaa ctcatcgaa ggccaccag tgggtctgg tccaacctg ggcgtgaag cccgtctcc ggctggtcc gtggacccg ttcgggcac ggcagcacc cagccctac atcctggcc tcctcgggg atcaagggc acggtcatc cagcgcatc cactacgcg tggaagcaa tggctcgcc ctgaagcag tacggcgac ttccgatgg gtccccacc tggagcccc gccagcccc tcggccgcg ctcctcacc cacaaccag cccttcgac atctactca atcaaacac agctgcggt ccgcacccc tacatttgc ctaaacttt gactttcgc aaagtcatc ggcgagtac acggagtac tccatcaag gcgcaggcc atcaccgac aagaacatc aaggagaag agcgagttg ctgctcgag cagtacgct cgcaccgga tccttgttc ccccacaac gtggtcttg atgccccta ggcgacgat ttccgctac aacgtggcg gaagaatgg gaccaacaa tacacaaac tacatcaaa cttatagac tacatcaac gcaaacaaa gacacttat aaggccgaa gtcgggttc gggaccccg aaagactac ttccgcgag ataatgaaa cgctacgat caatacccg acgcttaaa ggcgacttt ttcgtctac tcggacatt ttctcggaa ggccgtccg gcctactgg tccggctac ttcaccacg cgtcccttc atgaaaatc ctggaccgg gagttggag aacagcctg cgaagcgcc gagattttg tacacaatt gccctgaac aacgcccgc caaaacaaa ctgctcccg tacgtgaaa atcctggaa cgcgatttc gaaaagttg gtgcgtgcg cgtcggaac ttggggctc ttccagcac cacgacgcc atcacaggg acgagcaaa tcgttcgtg atgcgtgac tacgggctc aagctgttc gaggggatc cgggacacc gtcagtatc cagcagaac tcgctccag agtctcctg ctcccggac attccccta aagccgggc gagaacgcc attttgagc gacctggaa cgggagtcg ttcgagaag ctccccaaa aagacgccc atcgggctg gggccgcgt caagtgcgg aaaatcgtg ctgtacaac tcgctcgcc cagccccgg gagcaatta attcaagtg cggaccaac acgagtagt gtgaaaatt gtcgataat gaggggaac gaagtagtg tcacaggtc aacccagtg tgggacacg tccgagagc aacacgaaa ttgtggatc gcggcggac gagttcgag ctcgtcttc gtcgccaag ctcaaggaa ctgtctctc acgacctac agcctggtc tatgaggag aatgagacg agactggcc accatttat tgcaacaag tgtcaccgg aaggccagc attatcaca aacactgat agtacaatc gggaagcag aacgacgtc ttgaaatca aaatttgca atcaaaaac atacccacg ggtgatatc cagttggag aaccacaag ttcaagatt ttatttaac gggaataca gggtttatg aagacggtc acgcggaaa cacagcccc aaaatcatg cagtgcggg ctccagttc gccgcttat cgcagtgcg cagttccac tcgggggcc tacttattc atgccggac cccaacgag cgcgatatc gaaaaggac gttttgcag cagtacaag gaccagatg agcattatc atcacttca ggcccggtc tcaagcgag atcacggtc atttacggg gctttcctc gtgcacagc gtggccatt taccacgtg gagacgccc ctggccgac gccatctac accgagagc accgttgat ttcgaaaac ccgcccaaa aaccgcgag actgagctt tttatgcgc ataatatcc gacgtgcaa aacggcgaa gtgcccgaa ttctacacc gatcagaac ggcttccag atgctgaaa cgggtcaaa gtggagaaa atcgggatc gagggcaat tatttcccc gtgacgacg atggcgtac atccaggac gacaacgtg cggctgagt ttgctgact gaccatgcg cagggggcg tccgggtgg cagcccggg ttcctggag gtgatgctt gacaggcgg acgctttac gacgactcc cgggggatg ggcgagggg ctggtggat aacagaaag acggtgaac aggttctgg atcttgatt gaggatgtg gtgaaggag aagggcgag aaggggcac tcgaagcgg ttcgagaac tcggacgac acgttcgag aaaggggac gttgttagg tcgtttaac gtgccgacg gatccggtg gaggccggg aagcaggag gggtttccc cgggcgtcg ctgttcgcc aatcggatg tcgaacggg ctgaactac cccgtgggg atctttatc cttgaccac gaacaccag gaagtgaag agcctcgat ttggtcagg agggagttc ccctgtgat acgcatttg ttgacgttg aggacgcaa ccggactcg gtttattcg cagtttccc tcttccagc gccctttta gtgctacat aggcaaggc tacgactgt gctgtgagt aggaatttc tcttgttcc ttgtcgaag ttcgacagc gggttcgag catgtcaaa gttaaggaa attcaagcg aagacgctg accgggatc gaggatttg ccagataat gttaagcac ttgagtgat atttatctc gaacccatg actttaaag acttttaac atgaccttt gtatag
- >TC014283 RA
gattgaatg aaaatttct cttctgcga gagatctcg ccagtgcgc cagtactgt tggtgcgct agtcgtaca gtagtccta aacaaaaca gttgtgaca gagatggcg gtggaagtg cacgcattc tgatgtttt accataaac ataacaata catttaaat aaccgcggg tgttaattt caattcgaa tttatttat gtcgttagg gaattaaaa tatcaaaaa cggtgcctg aattatcac acacaagga agagatatg atctgggct tggcagcgt catcgccga agatttaat tcactcatc cacagctct ccagtccgt cgataattt tagggttaa ttagtttga tgcaaacgg tctgatgat gtcggtgtc ggcgaaagt atgccgaaa aatgtctcc aaagacctt cgccttgac ggccatagg cctcacaat aatcctgtg cgtgtacta cgccaatta cacgacaga cattcgcac aaatcaaac gcaagaaaa ttcaaaaga aagcgacga actgcccca acctcagcg catcttcga gctcctcca aagatcaaa attcgcaac taataaaga gttcgaagt gtgtccgaa gctgtatcc tgccgaggc cgacataag cacagtgga tgtgtttaa ggattttga gtttcagcc gacttggat gaagagcaa agagtactg ggacaagtc ctttgaaga tcgatttga acgccaaaa aatggacgc tgaaaggcc accactgaa ggtgataat cgttcctca ctcccataa cgaccccgg atggctgaa aaccttcga gaattactt ccactacgt ctcccgcca gataatgaa ccacatggt ggcgaaact ccagcagta caaaaacct aacattcat ttggtcgga agtggcctt tctgaacgc ctggtggga ggaggccca tcccagtaa acagcgagc tttgcgaaa cttggtcca ctcagggcg cctcgaaat cgccaccgg tggctgggt gatgaccga cgaagccaa cgctcacgt ctacgccat ggtggacca actcatcga aggccacca gtgggtctg gtccaacct gggcgtgaa gcccgtctc cggctggtc cgtggaccc gttcgggca cggcagcac ccagcccta catcctggc ctcctcggg gatcaaggg cacggtcat ccagcgcat ccactacgc gtggaagca atggctcgc cctgaagca gtacggcga cttccgatg ggtccccac ctggagccc cgccagccc ctcggccgc gctcctcac ccacaacca gcccttcga catctactc aatcaaaca cagctgcgg tccgcaccc ctacatttg cctaaactt tgactttcg caaagtcat cggcgagta cacggagta ctccatcaa ggcgcaggc catcaccga caagaacat caaggagaa gagcgagtt gctgctcga gcagtacgc tcgcaccgg atccttgtt cccccacaa cgtggtctt gatgcccct aggcgacga tttccgcta caacgtggc ggaagaatg ggaccaaca atacacaaa ctacatcaa acttataga ctacatcaa cgcaaacaa agacactta taaggccga agtcgggtt cgggacccc gaaagacta cttccgcga gataatgaa acgctacga tcaataccc gacgcttaa aggcgactt tttcgtcta ctcggacat tttctcgga aggccgtcc ggcctactg gtccggcta cttcaccac gcgtccctt catgaaaat cctggaccg ggagttgga gaacagcct gcgaagcgc cgagatttt gtacacaat tgccctgaa caacgcccg ccaaaacaa actgctccc gtacgtgaa aatcctgga acgcgattt cgaaaagtt ggtgcgtgc gcgtcggaa cttggggct cttccagca ccacgacgc catcacagg gacgagcaa atcgttcgt gatgcgtga ctacgggct caagctgtt cgaggggat ccgggacac cgtcagtat ccagcagaa ctcgctcca gagtctcct gctcccgga cattcccct aaagccggg cgagaacgc cattttgag cgacctgga acgggagtc gttcgagaa gctccccaa aaagacgcc catcgggct ggggccgcg tcaagtgcg gaaaatcgt gctgtacaa ctcgctcgc ccagccccg ggagcaatt aattcaagt gcggaccaa cacgagtag tgtgaaaat tgtcgataa tgaggggaa cgaagtagt gtcacaggt caacccagt gtgggacac gtccgagag caacacgaa attgtggat cgcggcgga cgagttcga gctcgtctt cgtcgccaa gctcaagga actgtctct cacgaccta cagcctggt ctatgagga gaatgagac gagactggc caccattta ttgcaacaa gtgtcaccg gaaggccag cattatcac aaacactga tagtacaat cgggaagca gaacgacgt cttgaaatc aaaatttgc aatcaaaaa catacccac gggtgatat ccagttgga gaaccacaa gttcaagat tttatttaa cgggaatac agggtttat gaagacggt cacgcggaa acacagccc caaaatcat gcagtgcgg gctccagtt cgccgctta tcgcagtgc gcagttcca ctcgggggc ctacttatt catgccgga ccccaacga gcgcgatat cgaaaagga cgttttgca gcagtacaa ggaccagat gagcattat catcacttc aggcccggt ctcaagcga gatcacggt catttacgg ggctttcct cgtgcacag cgtggccat ttaccacgt ggagacgcc cctggccga cgccatcta caccgagag caccgttga tttcgaaaa cccgcccaa aaaccgcga gactgagct ttttatgcg cataatatc cgacgtgca aaacggcga agtgcccga attctacac cgatcagaa cggcttcca gatgctgaa acgggtcaa agtggagaa aatcgggat cgagggcaa ttatttccc cgtgacgac gatggcgta catccagga cgacaacgt gcggctgag tttgctgac tgaccatgc gcagggggc gtccgggtg gcagcccgg gttcctgga ggtgatgct tgacaggcg gacgcttta cgacgactc ccgggggat gggcgaggg gctggtgga taacagaaa gacggtgaa caggttctg gatcttgat tgaggatgt ggtgaagga gaagggcga gaaggggca ctcgaagcg gttcgagaa ctcggacga cacgttcga gaaagggga cgttgttag gtcgtttaa cgtgccgac ggatccggt ggaggccgg gaagcagga ggggtttcc ccgggcgtc gctgttcgc caatcggat gtcgaacgg gctgaacta ccccgtggg gatctttat ccttgacca cgaacacca ggaagtgaa gagcctcga tttggtcag gagggagtt cccctgtga tacgcattt gttgacgtt gaggacgca accggactc ggtttattc gcagtttcc ctcttccag cgccctttt agtgctaca taggcaagg ctacgactg tgctgtgag taggaattt ctcttgttc cttgtcgaa gttcgacag cgggttcga gcatgtcaa agttaagga aattcaagc gaagacgct gaccgggat cgaggattt gccagataa tgttaagca cttgagtga tatttatct cgaacccat gactttaaa gacttttaa catgacctt tgtatagta gcaattgat gtgatttta tagattttt aatgaatac tttatttat atttttaaa atttgtaga tagtgtaaa taggacttt ttaaaaatg tgtgcattt tatgttgtt acgtcctcg tagaattat tgggtttta tgtaatttc aagcactga ctgatcatg ttttattat ttaagtatg tttgctgtt acgaaaaaa caaatatac cgtttttta tacgttgaa ccttatttg ccgaaataa aacttccaa caaatg
