Status
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Gene information
General information
TC014283 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8370955..8391530
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014283
    iBeetle screen iB_06327

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature dorsal not fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014283 RA
      MSPKTFALT AIGLTIILC VYYANYTTD IRTNQTQEN 
      SKESDELPQ PQRIFELLQ RSKFATNKE FEVCPKLYP 
      AEADISTVD VFKDFEFQP TWMKSKEYW DKSFEDRFE 
      RQKMDAERP PLKVIIVPH SHNDPGWLK TFENYFHYV 
      SRQIMNHMV AKLQQYKNL TFIWSEVAF LNAWWEEAH 
      PSKQRALRN LVHSGRLEI ATGGWVMTD EANAHVYAM 
      VDQLIEGHQ WVWSNLGVK PVSGWSVDP FGHGSTQPY 
      ILASSGIKG TVIQRIHYA WKQWLALKQ YGDFRWVPT 
      WSPASPSAA LLTHNQPFD IYSIKHSCG PHPYICLNF 
      DFRKVIGEY TEYSIKAQA ITDKNIKEK SELLLEQYA 
      RTGSLFPHN VVLMPLGDD FRYNVAEEW DQQYTNYIK 
      LIDYINANK DTYKAEVGF GTPKDYFRE IMKRYDQYP 
      TLKGDFFVY SDIFSEGRP AYWSGYFTT RPFMKILDR 
      ELENSLRSA EILYTIALN NARQNKLLP YVKILERDF 
      EKLVRARRN LGLFQHHDA ITGTSKSFV MRDYGLKLF 
      EGIRDTVSI QQNSLQSLL LPDIPLKPG ENAILSDLE 
      RESFEKLPK KTPIGLGPR QVRKIVLYN SLAQPREQL 
      IQVRTNTSS VKIVDNEGN EVVSQVNPV WDTSESNTK 
      LWIAADEFE LVFVAKLKE LSLTTYSLV YEENETRLA 
      TIYCNKCHR KASIITNTD STIGKQNDV LKSKFAIKN 
      IPTGDIQLE NHKFKILFN GNTGFMKTV TRKHSPKIM 
      QCGLQFAAY RSAQFHSGA YLFMPDPNE RDIEKDVLQ 
      QYKDQMSII ITSGPVSSE ITVIYGAFL VHSVAIYHV 
      ETPLADAIY TESTVDFEN PPKNRETEL FMRIISDVQ 
      NGEVPEFYT DQNGFQMLK RVKVEKIGI EGNYFPVTT 
      MAYIQDDNV RLSLLTDHA QGASGWQPG FLEVMLDRR 
      TLYDDSRGM GEGLVDNRK TVNRFWILI EDVVKEKGE 
      KGHSKRFEN SDDTFEKGD VVRSFNVPT DPVEAGKQE 
      GFPRASLFA NRMSNGLNY PVGIFILDH EHQEVKSLD 
      LVRREFPCD THLLTLRTQ PDSVYSQFP SSSALLVLH 
      RQGYDCAVS RNFSCSLSK FDSGFEHVK VKEIQAKTL 
      TGIEDLPDN VKHLSDIYL EPMTLKTFN MTFV
    CDS sequence
    • >TC014283 RA
      atgtctcca aagaccttc gccttgacg gccataggc 
      ctcacaata atcctgtgc gtgtactac gccaattac 
      acgacagac attcgcaca aatcaaacg caagaaaat 
      tcaaaagaa agcgacgaa ctgccccaa cctcagcgc 
      atcttcgag ctcctccaa agatcaaaa ttcgcaact 
      aataaagag ttcgaagtg tgtccgaag ctgtatcct 
      gccgaggcc gacataagc acagtggat gtgtttaag 
      gattttgag tttcagccg acttggatg aagagcaaa 
      gagtactgg gacaagtcc tttgaagat cgatttgaa 
      cgccaaaaa atggacgct gaaaggcca ccactgaag 
      gtgataatc gttcctcac tcccataac gaccccgga 
      tggctgaaa accttcgag aattacttc cactacgtc 
      tcccgccag ataatgaac cacatggtg gcgaaactc 
      cagcagtac aaaaaccta acattcatt tggtcggaa 
      gtggccttt ctgaacgcc tggtgggag gaggcccat 
      cccagtaaa cagcgagct ttgcgaaac ttggtccac 
      tcagggcgc ctcgaaatc gccaccggt ggctgggtg 
      atgaccgac gaagccaac gctcacgtc tacgccatg 
      gtggaccaa ctcatcgaa ggccaccag tgggtctgg 
      tccaacctg ggcgtgaag cccgtctcc ggctggtcc 
      gtggacccg ttcgggcac ggcagcacc cagccctac 
      atcctggcc tcctcgggg atcaagggc acggtcatc 
      cagcgcatc cactacgcg tggaagcaa tggctcgcc 
      ctgaagcag tacggcgac ttccgatgg gtccccacc 
      tggagcccc gccagcccc tcggccgcg ctcctcacc 
      cacaaccag cccttcgac atctactca atcaaacac 
      agctgcggt ccgcacccc tacatttgc ctaaacttt 
      gactttcgc aaagtcatc ggcgagtac acggagtac 
      tccatcaag gcgcaggcc atcaccgac aagaacatc 
      aaggagaag agcgagttg ctgctcgag cagtacgct 
      cgcaccgga tccttgttc ccccacaac gtggtcttg 
      atgccccta ggcgacgat ttccgctac aacgtggcg 
      gaagaatgg gaccaacaa tacacaaac tacatcaaa 
      cttatagac tacatcaac gcaaacaaa gacacttat 
      aaggccgaa gtcgggttc gggaccccg aaagactac 
      ttccgcgag ataatgaaa cgctacgat caatacccg 
      acgcttaaa ggcgacttt ttcgtctac tcggacatt 
      ttctcggaa ggccgtccg gcctactgg tccggctac 
      ttcaccacg cgtcccttc atgaaaatc ctggaccgg 
      gagttggag aacagcctg cgaagcgcc gagattttg 
      tacacaatt gccctgaac aacgcccgc caaaacaaa 
      ctgctcccg tacgtgaaa atcctggaa cgcgatttc 
      gaaaagttg gtgcgtgcg cgtcggaac ttggggctc 
      ttccagcac cacgacgcc atcacaggg acgagcaaa 
      tcgttcgtg atgcgtgac tacgggctc aagctgttc 
      gaggggatc cgggacacc gtcagtatc cagcagaac 
      tcgctccag agtctcctg ctcccggac attccccta 
      aagccgggc gagaacgcc attttgagc gacctggaa 
      cgggagtcg ttcgagaag ctccccaaa aagacgccc 
      atcgggctg gggccgcgt caagtgcgg aaaatcgtg 
      ctgtacaac tcgctcgcc cagccccgg gagcaatta 
      attcaagtg cggaccaac acgagtagt gtgaaaatt 
      gtcgataat gaggggaac gaagtagtg tcacaggtc 
      aacccagtg tgggacacg tccgagagc aacacgaaa 
      ttgtggatc gcggcggac gagttcgag ctcgtcttc 
      gtcgccaag ctcaaggaa ctgtctctc acgacctac 
      agcctggtc tatgaggag aatgagacg agactggcc 
      accatttat tgcaacaag tgtcaccgg aaggccagc 
      attatcaca aacactgat agtacaatc gggaagcag 
      aacgacgtc ttgaaatca aaatttgca atcaaaaac 
      atacccacg ggtgatatc cagttggag aaccacaag 
      ttcaagatt ttatttaac gggaataca gggtttatg 
      aagacggtc acgcggaaa cacagcccc aaaatcatg 
      cagtgcggg ctccagttc gccgcttat cgcagtgcg 
      cagttccac tcgggggcc tacttattc atgccggac 
      cccaacgag cgcgatatc gaaaaggac gttttgcag 
      cagtacaag gaccagatg agcattatc atcacttca 
      ggcccggtc tcaagcgag atcacggtc atttacggg 
      gctttcctc gtgcacagc gtggccatt taccacgtg 
      gagacgccc ctggccgac gccatctac accgagagc 
      accgttgat ttcgaaaac ccgcccaaa aaccgcgag 
      actgagctt tttatgcgc ataatatcc gacgtgcaa 
      aacggcgaa gtgcccgaa ttctacacc gatcagaac 
      ggcttccag atgctgaaa cgggtcaaa gtggagaaa 
      atcgggatc gagggcaat tatttcccc gtgacgacg 
      atggcgtac atccaggac gacaacgtg cggctgagt 
      ttgctgact gaccatgcg cagggggcg tccgggtgg 
      cagcccggg ttcctggag gtgatgctt gacaggcgg 
      acgctttac gacgactcc cgggggatg ggcgagggg 
      ctggtggat aacagaaag acggtgaac aggttctgg 
      atcttgatt gaggatgtg gtgaaggag aagggcgag 
      aaggggcac tcgaagcgg ttcgagaac tcggacgac 
      acgttcgag aaaggggac gttgttagg tcgtttaac 
      gtgccgacg gatccggtg gaggccggg aagcaggag 
      gggtttccc cgggcgtcg ctgttcgcc aatcggatg 
      tcgaacggg ctgaactac cccgtgggg atctttatc 
      cttgaccac gaacaccag gaagtgaag agcctcgat 
      ttggtcagg agggagttc ccctgtgat acgcatttg 
      ttgacgttg aggacgcaa ccggactcg gtttattcg 
      cagtttccc tcttccagc gccctttta gtgctacat 
      aggcaaggc tacgactgt gctgtgagt aggaatttc 
      tcttgttcc ttgtcgaag ttcgacagc gggttcgag 
      catgtcaaa gttaaggaa attcaagcg aagacgctg 
      accgggatc gaggatttg ccagataat gttaagcac 
      ttgagtgat atttatctc gaacccatg actttaaag 
      acttttaac atgaccttt gtatag
    mRNA sequence
    • >TC014283 RA
      gattgaatg aaaatttct cttctgcga gagatctcg 
      ccagtgcgc cagtactgt tggtgcgct agtcgtaca 
      gtagtccta aacaaaaca gttgtgaca gagatggcg 
      gtggaagtg cacgcattc tgatgtttt accataaac 
      ataacaata catttaaat aaccgcggg tgttaattt 
      caattcgaa tttatttat gtcgttagg gaattaaaa 
      tatcaaaaa cggtgcctg aattatcac acacaagga 
      agagatatg atctgggct tggcagcgt catcgccga 
      agatttaat tcactcatc cacagctct ccagtccgt 
      cgataattt tagggttaa ttagtttga tgcaaacgg 
      tctgatgat gtcggtgtc ggcgaaagt atgccgaaa 
      aatgtctcc aaagacctt cgccttgac ggccatagg 
      cctcacaat aatcctgtg cgtgtacta cgccaatta 
      cacgacaga cattcgcac aaatcaaac gcaagaaaa 
      ttcaaaaga aagcgacga actgcccca acctcagcg 
      catcttcga gctcctcca aagatcaaa attcgcaac 
      taataaaga gttcgaagt gtgtccgaa gctgtatcc 
      tgccgaggc cgacataag cacagtgga tgtgtttaa 
      ggattttga gtttcagcc gacttggat gaagagcaa 
      agagtactg ggacaagtc ctttgaaga tcgatttga 
      acgccaaaa aatggacgc tgaaaggcc accactgaa 
      ggtgataat cgttcctca ctcccataa cgaccccgg 
      atggctgaa aaccttcga gaattactt ccactacgt 
      ctcccgcca gataatgaa ccacatggt ggcgaaact 
      ccagcagta caaaaacct aacattcat ttggtcgga 
      agtggcctt tctgaacgc ctggtggga ggaggccca 
      tcccagtaa acagcgagc tttgcgaaa cttggtcca 
      ctcagggcg cctcgaaat cgccaccgg tggctgggt 
      gatgaccga cgaagccaa cgctcacgt ctacgccat 
      ggtggacca actcatcga aggccacca gtgggtctg 
      gtccaacct gggcgtgaa gcccgtctc cggctggtc 
      cgtggaccc gttcgggca cggcagcac ccagcccta 
      catcctggc ctcctcggg gatcaaggg cacggtcat 
      ccagcgcat ccactacgc gtggaagca atggctcgc 
      cctgaagca gtacggcga cttccgatg ggtccccac 
      ctggagccc cgccagccc ctcggccgc gctcctcac 
      ccacaacca gcccttcga catctactc aatcaaaca 
      cagctgcgg tccgcaccc ctacatttg cctaaactt 
      tgactttcg caaagtcat cggcgagta cacggagta 
      ctccatcaa ggcgcaggc catcaccga caagaacat 
      caaggagaa gagcgagtt gctgctcga gcagtacgc 
      tcgcaccgg atccttgtt cccccacaa cgtggtctt 
      gatgcccct aggcgacga tttccgcta caacgtggc 
      ggaagaatg ggaccaaca atacacaaa ctacatcaa 
      acttataga ctacatcaa cgcaaacaa agacactta 
      taaggccga agtcgggtt cgggacccc gaaagacta 
      cttccgcga gataatgaa acgctacga tcaataccc 
      gacgcttaa aggcgactt tttcgtcta ctcggacat 
      tttctcgga aggccgtcc ggcctactg gtccggcta 
      cttcaccac gcgtccctt catgaaaat cctggaccg 
      ggagttgga gaacagcct gcgaagcgc cgagatttt 
      gtacacaat tgccctgaa caacgcccg ccaaaacaa 
      actgctccc gtacgtgaa aatcctgga acgcgattt 
      cgaaaagtt ggtgcgtgc gcgtcggaa cttggggct 
      cttccagca ccacgacgc catcacagg gacgagcaa 
      atcgttcgt gatgcgtga ctacgggct caagctgtt 
      cgaggggat ccgggacac cgtcagtat ccagcagaa 
      ctcgctcca gagtctcct gctcccgga cattcccct 
      aaagccggg cgagaacgc cattttgag cgacctgga 
      acgggagtc gttcgagaa gctccccaa aaagacgcc 
      catcgggct ggggccgcg tcaagtgcg gaaaatcgt 
      gctgtacaa ctcgctcgc ccagccccg ggagcaatt 
      aattcaagt gcggaccaa cacgagtag tgtgaaaat 
      tgtcgataa tgaggggaa cgaagtagt gtcacaggt 
      caacccagt gtgggacac gtccgagag caacacgaa 
      attgtggat cgcggcgga cgagttcga gctcgtctt 
      cgtcgccaa gctcaagga actgtctct cacgaccta 
      cagcctggt ctatgagga gaatgagac gagactggc 
      caccattta ttgcaacaa gtgtcaccg gaaggccag 
      cattatcac aaacactga tagtacaat cgggaagca 
      gaacgacgt cttgaaatc aaaatttgc aatcaaaaa 
      catacccac gggtgatat ccagttgga gaaccacaa 
      gttcaagat tttatttaa cgggaatac agggtttat 
      gaagacggt cacgcggaa acacagccc caaaatcat 
      gcagtgcgg gctccagtt cgccgctta tcgcagtgc 
      gcagttcca ctcgggggc ctacttatt catgccgga 
      ccccaacga gcgcgatat cgaaaagga cgttttgca 
      gcagtacaa ggaccagat gagcattat catcacttc 
      aggcccggt ctcaagcga gatcacggt catttacgg 
      ggctttcct cgtgcacag cgtggccat ttaccacgt 
      ggagacgcc cctggccga cgccatcta caccgagag 
      caccgttga tttcgaaaa cccgcccaa aaaccgcga 
      gactgagct ttttatgcg cataatatc cgacgtgca 
      aaacggcga agtgcccga attctacac cgatcagaa 
      cggcttcca gatgctgaa acgggtcaa agtggagaa 
      aatcgggat cgagggcaa ttatttccc cgtgacgac 
      gatggcgta catccagga cgacaacgt gcggctgag 
      tttgctgac tgaccatgc gcagggggc gtccgggtg 
      gcagcccgg gttcctgga ggtgatgct tgacaggcg 
      gacgcttta cgacgactc ccgggggat gggcgaggg 
      gctggtgga taacagaaa gacggtgaa caggttctg 
      gatcttgat tgaggatgt ggtgaagga gaagggcga 
      gaaggggca ctcgaagcg gttcgagaa ctcggacga 
      cacgttcga gaaagggga cgttgttag gtcgtttaa 
      cgtgccgac ggatccggt ggaggccgg gaagcagga 
      ggggtttcc ccgggcgtc gctgttcgc caatcggat 
      gtcgaacgg gctgaacta ccccgtggg gatctttat 
      ccttgacca cgaacacca ggaagtgaa gagcctcga 
      tttggtcag gagggagtt cccctgtga tacgcattt 
      gttgacgtt gaggacgca accggactc ggtttattc 
      gcagtttcc ctcttccag cgccctttt agtgctaca 
      taggcaagg ctacgactg tgctgtgag taggaattt 
      ctcttgttc cttgtcgaa gttcgacag cgggttcga 
      gcatgtcaa agttaagga aattcaagc gaagacgct 
      gaccgggat cgaggattt gccagataa tgttaagca 
      cttgagtga tatttatct cgaacccat gactttaaa 
      gacttttaa catgacctt tgtatagta gcaattgat 
      gtgatttta tagattttt aatgaatac tttatttat 
      atttttaaa atttgtaga tagtgtaaa taggacttt 
      ttaaaaatg tgtgcattt tatgttgtt acgtcctcg 
      tagaattat tgggtttta tgtaatttc aagcactga 
      ctgatcatg ttttattat ttaagtatg tttgctgtt 
      acgaaaaaa caaatatac cgtttttta tacgttgaa 
      ccttatttg ccgaaataa aacttccaa caaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY