Status
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Gene information
General information
TC030884
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014224,TC014223 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3441373..3456014
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030884
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05590

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_06326

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 10 (41 dpi)
    Days post injection

    Pupal morphology
    labial palp not fused - Number of animals showing the phenotype: 10

    head orientation irregular - Number of animals showing the phenotype: 10

    elytra shortened - Number of animals showing the phenotype: 10

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030884 RA
      MSVSERESQ EGSALGDSS DSGKMYRRL KVPDCKHSA 
LLPLVNIDK ENYMLRGVS RPGSEVDLD SFMVCEINS 
TLSALNVMA GQKEAAAAD VDADVDVDG PGAGFCARI 
VSFHPLMCS CEMSCCCMC GSFCSSDCF GCFHDMCAR 
FAAKHQCLK NEEVPTTTA TLEHDKPIS EWTSAQVLE 
WMAATNLYL CSDIFRYKD IKGADLVHL DKEKLINMG 
IKDEFHQMA ILSCIEELL GKQENKMLS NDQDSDIAH 
THNLTQHSF SSLERCGKC NKYLRGLLH QGFVCQDCG 
LVAHRSCAA TGLPPCTSA FGRGLCLQF FPGDMQEAP 
AFLVKFIDE LESKAENDE TLELYNLYS ATPPPDQMA 
KLLEKINDG STNLDLSDF NAVCIAGII KKFLRELPD 
PLVPVQWYD AFLTASKAK TDEACANQL TRLVEELPE 
NHRSTLKFV MAHLCRMCQ MEYSRGNHS PPTVLIQAL 
CHIFLRPPW ERIIQVVYN TQSHNRILE VLLLKCSWG 
VKLPEFASA PAIPPRRAV GTARISTNS TLDKEKSNA 
FSLQEAEWY WGDIKREEV NEKLKETPD GTFLVRDAS 
TKSGEYTLT LRKGGTNKL IKICHKNGK YGFTEPYTF 
NSVVELINH FRNESLSQY NASLDIKLL YPVSMFNHE 
EEAAKTEDV EKLWDHLRD IKNTLMNKN KVLDSLTED 
LNKTSQEVS TKRQALEAL KELVKVFQE QIATHAQVK 
KEAQPHEIV GLTVNEKLL TQRLKMMQE SSEHLDKTL 
EEKEAYNRT LERELTALK PVVHSLRKE RDKYIRWLQ 
ERGVSPTKI NQVLNRNSE DEEYNTQSG EECDVDSLI 
HNDERTWLM LDCRRIDAE KYLAGQPDG TFLIRTSST 
SKYALSIAC NKMVNHYLV LHYATNSLE IHNDSLNTV 
LAHPLLAWK YSSNQDRYI GGSSTTYN
    CDS sequence
    • >TC030884 RA
      atgagcgtt tcggaacgt gaaagccag gagggcagt 
gctttgggc gactcctcc gacagcggt aaaatgtac 
cggcgcttg aaagtgccg gactgcaag cactccgca 
ctcctgccg ctggtcaac atcgacaaa gagaattac 
atgttgcga ggggtcagt cgtccgggc tctgaagtc 
gatctcgac agcttcatg gtgtgcgag atcaacagc 
acgctgagc gcgctgaac gtgatggcc gggcagaag 
gaggccgcc gccgccgac gtcgatgcg gacgtggac 
gtcgacggc ccaggcgcc ggcttttgc gctcgcatc 
gtctcgttc catccgctg atgtgctcc tgcgaaatg 
tcctgctgc tgcatgtgc ggttcgttc tgttcttca 
gattgcttc ggctgcttc cacgacatg tgcgcgagg 
ttcgccgcc aaacaccaa tgtcttaag aacgaggaa 
gtgcccacg acgacggcg acgctcgag cacgacaag 
cccatatcc gaatggacg tcggcccaa gttctggaa 
tggatggcg gcgaccaac ctctacctc tgctcggac 
atcttccga tacaaagac atcaaaggc gctgatttg 
gttcatctg gacaaagaa aaattaatt aatatgggc 
atcaaggac gagttccac caaatggcg atcctgtcg 
tgcattgag gagcttttg gggaagcag gagaacaag 
atgttgagc aacgaccaa gattcggat atagcgcac 
acgcataac ttgacgcag catagtttc agcagttta 
gaacgttgc ggaaaatgc aacaaatac ttgcgaggt 
ttgcttcat cagggtttc gtttgtcag gactgtggt 
ctggtggct catcgctcg tgtgccgcc accggtctg 
ccgccttgc acgtccgcc ttcggtcgg gggctgtgt 
ctccagttt ttccccgga gacatgcag gaagcgccc 
gcttttctc gtaaagttc atcgacgaa ttggagtcg 
aaagccgag aacgacgaa actctagaa ctgtataat 
ctctacagc gccacacct ccgcctgat caaatggcc 
aagctgctc gagaaaatc aacgacggt tcaaccaat 
ttagattta agcgatttt aacgccgtt tgtatagcc 
ggaattatc aaaaagttt cttagggag ttacccgat 
cctcttgta cctgtccag tggtacgat gcgttttta 
acagcgtca aaagctaaa actgacgaa gcttgcgcg 
aaccaattg acacgttta gtcgaagag ttgcctgaa 
aatcatcgt tccacgtta aaatttgtg atggctcat 
ttgtgtcgt atgtgccag atggaatac tccaggggc 
aatcacagt ccccctact gttctaatc caggcatta 
tgccacatt tttctgaga ccaccttgg gaacgtatt 
attcaagtg gtctacaat actcagtct cataatagg 
attttagaa gtactactt ttgaagtgt agttggggc 
gtcaagttg cctgaattt gcctcagct ccggccatt 
ccgccgcgt cgggcggtg ggaacggcc cgaatttca 
acaaacagc acactagat aaggaaaaa tcgaacgcc 
ttctcgctc caagaggcg gaatggtac tggggcgac 
ataaagcgg gaagaagtc aacgagaaa ctgaaagaa 
accccggac ggtacgttt ttagtgcgc gacgcgtca 
acaaaaagt ggcgagtac acattgacc cttcgtaag 
ggtggtaca aacaagcta attaaaatt tgccataaa 
aacgggaag tacggtttc accgaaccg tacaccttt 
aactcggtc gtggaattg ataaaccat tttcgtaac 
gagtcttta tcacaatat aacgcctcc ttagatata 
aagttgttg tatccggtg tctatgttt aaccacgag 
gaggaagcg gcgaaaacg gaggacgtg gagaagctc 
tgggatcac ttacgcgac atcaagaac actctcatg 
aacaagaac aaagtcttg gacagcctg actgaagat 
ctgaacaag acaagccag gaggtctcc accaaacgc 
caggccctt gaagcttta aaagagttg gttaaagtg 
ttccaagaa cagatcgcc actcacgca caagtcaag 
aaagaggca caaccgcac gaaatcgtg gggttaacc 
gtcaacgag aaactacta acgcaacga ctgaaaatg 
atgcaggag agctccgag cacttagat aagacttta 
gaggagaaa gaggcctac aaccggacg cttgagcgc 
gaactgacc gccctgaag ccggtcgtg cacagtctg 
cgcaaagaa cgggacaaa tacattagg tggcttcag 
gagcgcggc gtgagtccg acgaagatc aaccaggtg 
ctgaaccgc aacagcgag gacgaggag tacaacacg 
cagtcgggc gaagagtgc gatgtggac tcgctgata 
cataacgac gagcgcact tggctgatg ctggactgc 
cggcgaatc gatgccgag aagtatttg gccggccag 
ccggacggg acgttcctg atccgtact tcgagcact 
tcgaaatac gccctgtcc attgcttgc aacaagatg 
gtcaaccac tatctggtc ttgcactac gcgacgaat 
tccttggag atacacaac gattctttg aacacggtc 
ttagcgcat ccgcttctc gcctggaag tacagcagt 
aaccaagat cgctacatc ggaggatca agcaccacg 
tacaactaa
    mRNA sequence
    • >TC030884 RA
      tccgaaacg agaactggc tagtttcgt cgaggtagt 
cgtgagtta atgttgtct taagaagcg ataaattcg 
caaacggtg gtaacatga caagatgag cgtttcgga 
acgtgaaag ccaggaggg cagtgcttt gggcgactc 
ctccgacag cggtaaaat gtaccggcg cttgaaagt 
gccggactg caagcactc cgcactcct gccgctggt 
caacatcga caaagagaa ttacatgtt gcgaggggt 
cagtcgtcc gggctctga agtcgatct cgacagctt 
catggtgtg cgagatcaa cagcacgct gagcgcgct 
gaacgtgat ggccgggca gaaggaggc cgccgccgc 
cgacgtcga tgcggacgt ggacgtcga cggcccagg 
cgccggctt ttgcgctcg catcgtctc gttccatcc 
gctgatgtg ctcctgcga aatgtcctg ctgctgcat 
gtgcggttc gttctgttc ttcagattg cttcggctg 
cttccacga catgtgcgc gaggttcgc cgccaaaca 
ccaatgtct taagaacga ggaagtgcc cacgacgac 
ggcgacgct cgagcacga caagcccat atccgaatg 
gacgtcggc ccaagttct ggaatggat ggcggcgac 
caacctcta cctctgctc ggacatctt ccgatacaa 
agacatcaa aggcgctga tttggttca tctggacaa 
agaaaaatt aattaatat gggcatcaa ggacgagtt 
ccaccaaat ggcgatcct gtcgtgcat tgaggagct 
tttggggaa gcaggagaa caagatgtt gagcaacga 
ccaagattc ggatatagc gcacacgca taacttgac 
gcagcatag tttcagcag tttagaacg ttgcggaaa 
atgcaacaa atacttgcg aggtttgct tcatcaggg 
tttcgtttg tcaggactg tggtctggt ggctcatcg 
ctcgtgtgc cgccaccgg tctgccgcc ttgcacgtc 
cgccttcgg tcgggggct gtgtctcca gtttttccc 
cggagacat gcaggaagc gcccgcttt tctcgtaaa 
gttcatcga cgaattgga gtcgaaagc cgagaacga 
cgaaactct agaactgta taatctcta cagcgccac 
acctccgcc tgatcaaat ggccaagct gctcgagaa 
aatcaacga cggttcaac caatttaga tttaagcga 
ttttaacgc cgtttgtat agccggaat tatcaaaaa 
gtttcttag ggagttacc cgatcctct tgtacctgt 
ccagtggta cgatgcgtt tttaacagc gtcaaaagc 
taaaactga cgaagcttg cgcgaacca attgacacg 
tttagtcga agagttgcc tgaaaatca tcgttccac 
gttaaaatt tgtgatggc tcatttgtg tcgtatgtg 
ccagatgga atactccag gggcaatca cagtccccc 
tactgttct aatccaggc attatgcca catttttct 
gagaccacc ttgggaacg tattattca agtggtcta 
caatactca gtctcataa taggatttt agaagtact 
acttttgaa gtgtagttg gggcgtcaa gttgcctga 
atttgcctc agctccggc cattccgcc gcgtcgggc 
ggtgggaac ggcccgaat ttcaacaaa cagcacact 
agataagga aaaatcgaa cgccttctc gctccaaga 
ggcggaatg gtactgggg cgacataaa gcgggaaga 
agtcaacga gaaactgaa agaaacccc ggacggtac 
gtttttagt gcgcgacgc gtcaacaaa aagtggcga 
gtacacatt gacccttcg taagggtgg tacaaacaa 
gctaattaa aatttgcca taaaaacgg gaagtacgg 
tttcaccga accgtacac ctttaactc ggtcgtgga 
attgataaa ccattttcg taacgagtc tttatcaca 
atataacgc ctccttaga tataaagtt gttgtatcc 
ggtgtctat gtttaacca cgaggagga agcggcgaa 
aacggagga cgtggagaa gctctggga tcacttacg 
cgacatcaa gaacactct catgaacaa gaacaaagt 
cttggacag cctgactga agatctgaa caagacaag 
ccaggaggt ctccaccaa acgccaggc ccttgaagc 
tttaaaaga gttggttaa agtgttcca agaacagat 
cgccactca cgcacaagt caagaaaga ggcacaacc 
gcacgaaat cgtggggtt aaccgtcaa cgagaaact 
actaacgca acgactgaa aatgatgca ggagagctc 
cgagcactt agataagac tttagagga gaaagaggc 
ctacaaccg gacgcttga gcgcgaact gaccgccct 
gaagccggt cgtgcacag tctgcgcaa agaacggga 
caaatacat taggtggct tcaggagcg cggcgtgag 
tccgacgaa gatcaacca ggtgctgaa ccgcaacag 
cgaggacga ggagtacaa cacgcagtc gggcgaaga 
gtgcgatgt ggactcgct gatacataa cgacgagcg 
cacttggct gatgctgga ctgccggcg aatcgatgc 
cgagaagta tttggccgg ccagccgga cgggacgtt 
cctgatccg tacttcgag cacttcgaa atacgccct 
gtccattgc ttgcaacaa gatggtcaa ccactatct 
ggtcttgca ctacgcgac gaattcctt ggagataca 
caacgattc tttgaacac ggtcttagc gcatccgct 
tctcgcctg gaagtacag cagtaacca agatcgcta 
catcggagg atcaagcac cacgtacaa ctaactgaa 
gagagtgat gtgataatt caaagttgc tgtgacttt 
tatattccc gtaatctta aattttacg atggtgata 
atttccaaa aactataac aaatttata taaagtaat 
gtatcatat aaatatatt tacatatac agtgacagt 
tttacaatt gaagtcgtt tttgcttaa tcaccaggt 
taaattgac tgtgatagt gtactaaag aagaagctc 
ttagattta tataacaac ctaagcaca tgtaagtgt 
gtgtgatgt gcggtgtat caatatggc ctggtttat 
ttttgaaat tcaacattc taggccttt acgtttgta 
aatatatga aattcaagc agtagcgta gtcacggga 
tatggagcc tgcagctgt ttgcgactc tagtcaatc 
gttaacctg cctctgttc aaacgcggt ggtgccatc 
tgatgcgtg gaagcttta ttattttcg taactattt 
tttgtgtta tggtgggcc actgctcac tacgctgct 
gccaaaatc gctgttagc cgatattca cttgctgcg 
ccaacgacg tgccaacgc ggcctattt ttgaattcg 
ttccgtatt tgcgcactg gatgatgct gttggcagg 
ttgttgggg tgtgttgtt aattcgcct gtcaatata 
tctacttat cttatttgc caagcgctg cagtgtgag 
aagaaagca ttctagata atattaata tgtgatttt 
atttaggta cctttttta gattgtgca atgtcttca 
aagtgtatt taactattc tttataaca ctcgtagta 
tttatttta aactaggat gtttttcat ttggaaagt 
acaagtgtt ttttacatt tgtttttaa aacaaatct 
catgtatct agaatggtt ttcgtattg ttttcactt 
ttttacaat cattatttg tagctgttt atctttaat 
tcattcttc catttttca tttattgtc aagaaagaa 
gagtattaa acttgttat atctagaat tttttattg 
ttgtttttc atttcttca aatttggag aggtacaaa 
atataattt atacgaact aatcttcta ttttatttt 
ggagctgca gttatcatt tgatgcttg agttgaaga 
attactgtg tgctttttt caaacttgt caatatctt 
caacattat tatatgtca gactgagtc tggaagtct 
ggtgcagta acgtagtta tataaaaga tagtgatgt 
ttttcattt ctattgtga ttaatggag acataatgg 
gtattcaaa tttaaacac aatgttctg taacgattt 
gatgataca tgtcaacag ttttttatt cttgtttgt 
atactttcg tttagttct taacgagag tcacacgtt 
ttcttgctt tcagtgtat gtaaaattt catgtacaa 
ttaataaat taatattag acta
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