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Gene information
General information
TC030884
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014224,TC014223
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3441373..3456014
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030884
    iBeetle screen iB_05590

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_06326

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 10 (41 dpi)
    Days post injection


    Pupal morphology
    labial palp not fused - Number of animals showing the phenotype: 10

    head orientation irregular - Number of animals showing the phenotype: 10

    elytra shortened - Number of animals showing the phenotype: 10

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030884 RA
      MSVSERESQ EGSALGDSS DSGKMYRRL KVPDCKHSA 
      LLPLVNIDK ENYMLRGVS RPGSEVDLD SFMVCEINS 
      TLSALNVMA GQKEAAAAD VDADVDVDG PGAGFCARI 
      VSFHPLMCS CEMSCCCMC GSFCSSDCF GCFHDMCAR 
      FAAKHQCLK NEEVPTTTA TLEHDKPIS EWTSAQVLE 
      WMAATNLYL CSDIFRYKD IKGADLVHL DKEKLINMG 
      IKDEFHQMA ILSCIEELL GKQENKMLS NDQDSDIAH 
      THNLTQHSF SSLERCGKC NKYLRGLLH QGFVCQDCG 
      LVAHRSCAA TGLPPCTSA FGRGLCLQF FPGDMQEAP 
      AFLVKFIDE LESKAENDE TLELYNLYS ATPPPDQMA 
      KLLEKINDG STNLDLSDF NAVCIAGII KKFLRELPD 
      PLVPVQWYD AFLTASKAK TDEACANQL TRLVEELPE 
      NHRSTLKFV MAHLCRMCQ MEYSRGNHS PPTVLIQAL 
      CHIFLRPPW ERIIQVVYN TQSHNRILE VLLLKCSWG 
      VKLPEFASA PAIPPRRAV GTARISTNS TLDKEKSNA 
      FSLQEAEWY WGDIKREEV NEKLKETPD GTFLVRDAS 
      TKSGEYTLT LRKGGTNKL IKICHKNGK YGFTEPYTF 
      NSVVELINH FRNESLSQY NASLDIKLL YPVSMFNHE 
      EEAAKTEDV EKLWDHLRD IKNTLMNKN KVLDSLTED 
      LNKTSQEVS TKRQALEAL KELVKVFQE QIATHAQVK 
      KEAQPHEIV GLTVNEKLL TQRLKMMQE SSEHLDKTL 
      EEKEAYNRT LERELTALK PVVHSLRKE RDKYIRWLQ 
      ERGVSPTKI NQVLNRNSE DEEYNTQSG EECDVDSLI 
      HNDERTWLM LDCRRIDAE KYLAGQPDG TFLIRTSST 
      SKYALSIAC NKMVNHYLV LHYATNSLE IHNDSLNTV 
      LAHPLLAWK YSSNQDRYI GGSSTTYN
    CDS sequence
    • >TC030884 RA
      atgagcgtt tcggaacgt gaaagccag gagggcagt 
      gctttgggc gactcctcc gacagcggt aaaatgtac 
      cggcgcttg aaagtgccg gactgcaag cactccgca 
      ctcctgccg ctggtcaac atcgacaaa gagaattac 
      atgttgcga ggggtcagt cgtccgggc tctgaagtc 
      gatctcgac agcttcatg gtgtgcgag atcaacagc 
      acgctgagc gcgctgaac gtgatggcc gggcagaag 
      gaggccgcc gccgccgac gtcgatgcg gacgtggac 
      gtcgacggc ccaggcgcc ggcttttgc gctcgcatc 
      gtctcgttc catccgctg atgtgctcc tgcgaaatg 
      tcctgctgc tgcatgtgc ggttcgttc tgttcttca 
      gattgcttc ggctgcttc cacgacatg tgcgcgagg 
      ttcgccgcc aaacaccaa tgtcttaag aacgaggaa 
      gtgcccacg acgacggcg acgctcgag cacgacaag 
      cccatatcc gaatggacg tcggcccaa gttctggaa 
      tggatggcg gcgaccaac ctctacctc tgctcggac 
      atcttccga tacaaagac atcaaaggc gctgatttg 
      gttcatctg gacaaagaa aaattaatt aatatgggc 
      atcaaggac gagttccac caaatggcg atcctgtcg 
      tgcattgag gagcttttg gggaagcag gagaacaag 
      atgttgagc aacgaccaa gattcggat atagcgcac 
      acgcataac ttgacgcag catagtttc agcagttta 
      gaacgttgc ggaaaatgc aacaaatac ttgcgaggt 
      ttgcttcat cagggtttc gtttgtcag gactgtggt 
      ctggtggct catcgctcg tgtgccgcc accggtctg 
      ccgccttgc acgtccgcc ttcggtcgg gggctgtgt 
      ctccagttt ttccccgga gacatgcag gaagcgccc 
      gcttttctc gtaaagttc atcgacgaa ttggagtcg 
      aaagccgag aacgacgaa actctagaa ctgtataat 
      ctctacagc gccacacct ccgcctgat caaatggcc 
      aagctgctc gagaaaatc aacgacggt tcaaccaat 
      ttagattta agcgatttt aacgccgtt tgtatagcc 
      ggaattatc aaaaagttt cttagggag ttacccgat 
      cctcttgta cctgtccag tggtacgat gcgttttta 
      acagcgtca aaagctaaa actgacgaa gcttgcgcg 
      aaccaattg acacgttta gtcgaagag ttgcctgaa 
      aatcatcgt tccacgtta aaatttgtg atggctcat 
      ttgtgtcgt atgtgccag atggaatac tccaggggc 
      aatcacagt ccccctact gttctaatc caggcatta 
      tgccacatt tttctgaga ccaccttgg gaacgtatt 
      attcaagtg gtctacaat actcagtct cataatagg 
      attttagaa gtactactt ttgaagtgt agttggggc 
      gtcaagttg cctgaattt gcctcagct ccggccatt 
      ccgccgcgt cgggcggtg ggaacggcc cgaatttca 
      acaaacagc acactagat aaggaaaaa tcgaacgcc 
      ttctcgctc caagaggcg gaatggtac tggggcgac 
      ataaagcgg gaagaagtc aacgagaaa ctgaaagaa 
      accccggac ggtacgttt ttagtgcgc gacgcgtca 
      acaaaaagt ggcgagtac acattgacc cttcgtaag 
      ggtggtaca aacaagcta attaaaatt tgccataaa 
      aacgggaag tacggtttc accgaaccg tacaccttt 
      aactcggtc gtggaattg ataaaccat tttcgtaac 
      gagtcttta tcacaatat aacgcctcc ttagatata 
      aagttgttg tatccggtg tctatgttt aaccacgag 
      gaggaagcg gcgaaaacg gaggacgtg gagaagctc 
      tgggatcac ttacgcgac atcaagaac actctcatg 
      aacaagaac aaagtcttg gacagcctg actgaagat 
      ctgaacaag acaagccag gaggtctcc accaaacgc 
      caggccctt gaagcttta aaagagttg gttaaagtg 
      ttccaagaa cagatcgcc actcacgca caagtcaag 
      aaagaggca caaccgcac gaaatcgtg gggttaacc 
      gtcaacgag aaactacta acgcaacga ctgaaaatg 
      atgcaggag agctccgag cacttagat aagacttta 
      gaggagaaa gaggcctac aaccggacg cttgagcgc 
      gaactgacc gccctgaag ccggtcgtg cacagtctg 
      cgcaaagaa cgggacaaa tacattagg tggcttcag 
      gagcgcggc gtgagtccg acgaagatc aaccaggtg 
      ctgaaccgc aacagcgag gacgaggag tacaacacg 
      cagtcgggc gaagagtgc gatgtggac tcgctgata 
      cataacgac gagcgcact tggctgatg ctggactgc 
      cggcgaatc gatgccgag aagtatttg gccggccag 
      ccggacggg acgttcctg atccgtact tcgagcact 
      tcgaaatac gccctgtcc attgcttgc aacaagatg 
      gtcaaccac tatctggtc ttgcactac gcgacgaat 
      tccttggag atacacaac gattctttg aacacggtc 
      ttagcgcat ccgcttctc gcctggaag tacagcagt 
      aaccaagat cgctacatc ggaggatca agcaccacg 
      tacaactaa
    mRNA sequence
    • >TC030884 RA
      tccgaaacg agaactggc tagtttcgt cgaggtagt 
      cgtgagtta atgttgtct taagaagcg ataaattcg 
      caaacggtg gtaacatga caagatgag cgtttcgga 
      acgtgaaag ccaggaggg cagtgcttt gggcgactc 
      ctccgacag cggtaaaat gtaccggcg cttgaaagt 
      gccggactg caagcactc cgcactcct gccgctggt 
      caacatcga caaagagaa ttacatgtt gcgaggggt 
      cagtcgtcc gggctctga agtcgatct cgacagctt 
      catggtgtg cgagatcaa cagcacgct gagcgcgct 
      gaacgtgat ggccgggca gaaggaggc cgccgccgc 
      cgacgtcga tgcggacgt ggacgtcga cggcccagg 
      cgccggctt ttgcgctcg catcgtctc gttccatcc 
      gctgatgtg ctcctgcga aatgtcctg ctgctgcat 
      gtgcggttc gttctgttc ttcagattg cttcggctg 
      cttccacga catgtgcgc gaggttcgc cgccaaaca 
      ccaatgtct taagaacga ggaagtgcc cacgacgac 
      ggcgacgct cgagcacga caagcccat atccgaatg 
      gacgtcggc ccaagttct ggaatggat ggcggcgac 
      caacctcta cctctgctc ggacatctt ccgatacaa 
      agacatcaa aggcgctga tttggttca tctggacaa 
      agaaaaatt aattaatat gggcatcaa ggacgagtt 
      ccaccaaat ggcgatcct gtcgtgcat tgaggagct 
      tttggggaa gcaggagaa caagatgtt gagcaacga 
      ccaagattc ggatatagc gcacacgca taacttgac 
      gcagcatag tttcagcag tttagaacg ttgcggaaa 
      atgcaacaa atacttgcg aggtttgct tcatcaggg 
      tttcgtttg tcaggactg tggtctggt ggctcatcg 
      ctcgtgtgc cgccaccgg tctgccgcc ttgcacgtc 
      cgccttcgg tcgggggct gtgtctcca gtttttccc 
      cggagacat gcaggaagc gcccgcttt tctcgtaaa 
      gttcatcga cgaattgga gtcgaaagc cgagaacga 
      cgaaactct agaactgta taatctcta cagcgccac 
      acctccgcc tgatcaaat ggccaagct gctcgagaa 
      aatcaacga cggttcaac caatttaga tttaagcga 
      ttttaacgc cgtttgtat agccggaat tatcaaaaa 
      gtttcttag ggagttacc cgatcctct tgtacctgt 
      ccagtggta cgatgcgtt tttaacagc gtcaaaagc 
      taaaactga cgaagcttg cgcgaacca attgacacg 
      tttagtcga agagttgcc tgaaaatca tcgttccac 
      gttaaaatt tgtgatggc tcatttgtg tcgtatgtg 
      ccagatgga atactccag gggcaatca cagtccccc 
      tactgttct aatccaggc attatgcca catttttct 
      gagaccacc ttgggaacg tattattca agtggtcta 
      caatactca gtctcataa taggatttt agaagtact 
      acttttgaa gtgtagttg gggcgtcaa gttgcctga 
      atttgcctc agctccggc cattccgcc gcgtcgggc 
      ggtgggaac ggcccgaat ttcaacaaa cagcacact 
      agataagga aaaatcgaa cgccttctc gctccaaga 
      ggcggaatg gtactgggg cgacataaa gcgggaaga 
      agtcaacga gaaactgaa agaaacccc ggacggtac 
      gtttttagt gcgcgacgc gtcaacaaa aagtggcga 
      gtacacatt gacccttcg taagggtgg tacaaacaa 
      gctaattaa aatttgcca taaaaacgg gaagtacgg 
      tttcaccga accgtacac ctttaactc ggtcgtgga 
      attgataaa ccattttcg taacgagtc tttatcaca 
      atataacgc ctccttaga tataaagtt gttgtatcc 
      ggtgtctat gtttaacca cgaggagga agcggcgaa 
      aacggagga cgtggagaa gctctggga tcacttacg 
      cgacatcaa gaacactct catgaacaa gaacaaagt 
      cttggacag cctgactga agatctgaa caagacaag 
      ccaggaggt ctccaccaa acgccaggc ccttgaagc 
      tttaaaaga gttggttaa agtgttcca agaacagat 
      cgccactca cgcacaagt caagaaaga ggcacaacc 
      gcacgaaat cgtggggtt aaccgtcaa cgagaaact 
      actaacgca acgactgaa aatgatgca ggagagctc 
      cgagcactt agataagac tttagagga gaaagaggc 
      ctacaaccg gacgcttga gcgcgaact gaccgccct 
      gaagccggt cgtgcacag tctgcgcaa agaacggga 
      caaatacat taggtggct tcaggagcg cggcgtgag 
      tccgacgaa gatcaacca ggtgctgaa ccgcaacag 
      cgaggacga ggagtacaa cacgcagtc gggcgaaga 
      gtgcgatgt ggactcgct gatacataa cgacgagcg 
      cacttggct gatgctgga ctgccggcg aatcgatgc 
      cgagaagta tttggccgg ccagccgga cgggacgtt 
      cctgatccg tacttcgag cacttcgaa atacgccct 
      gtccattgc ttgcaacaa gatggtcaa ccactatct 
      ggtcttgca ctacgcgac gaattcctt ggagataca 
      caacgattc tttgaacac ggtcttagc gcatccgct 
      tctcgcctg gaagtacag cagtaacca agatcgcta 
      catcggagg atcaagcac cacgtacaa ctaactgaa 
      gagagtgat gtgataatt caaagttgc tgtgacttt 
      tatattccc gtaatctta aattttacg atggtgata 
      atttccaaa aactataac aaatttata taaagtaat 
      gtatcatat aaatatatt tacatatac agtgacagt 
      tttacaatt gaagtcgtt tttgcttaa tcaccaggt 
      taaattgac tgtgatagt gtactaaag aagaagctc 
      ttagattta tataacaac ctaagcaca tgtaagtgt 
      gtgtgatgt gcggtgtat caatatggc ctggtttat 
      ttttgaaat tcaacattc taggccttt acgtttgta 
      aatatatga aattcaagc agtagcgta gtcacggga 
      tatggagcc tgcagctgt ttgcgactc tagtcaatc 
      gttaacctg cctctgttc aaacgcggt ggtgccatc 
      tgatgcgtg gaagcttta ttattttcg taactattt 
      tttgtgtta tggtgggcc actgctcac tacgctgct 
      gccaaaatc gctgttagc cgatattca cttgctgcg 
      ccaacgacg tgccaacgc ggcctattt ttgaattcg 
      ttccgtatt tgcgcactg gatgatgct gttggcagg 
      ttgttgggg tgtgttgtt aattcgcct gtcaatata 
      tctacttat cttatttgc caagcgctg cagtgtgag 
      aagaaagca ttctagata atattaata tgtgatttt 
      atttaggta cctttttta gattgtgca atgtcttca 
      aagtgtatt taactattc tttataaca ctcgtagta 
      tttatttta aactaggat gtttttcat ttggaaagt 
      acaagtgtt ttttacatt tgtttttaa aacaaatct 
      catgtatct agaatggtt ttcgtattg ttttcactt 
      ttttacaat cattatttg tagctgttt atctttaat 
      tcattcttc catttttca tttattgtc aagaaagaa 
      gagtattaa acttgttat atctagaat tttttattg 
      ttgtttttc atttcttca aatttggag aggtacaaa 
      atataattt atacgaact aatcttcta ttttatttt 
      ggagctgca gttatcatt tgatgcttg agttgaaga 
      attactgtg tgctttttt caaacttgt caatatctt 
      caacattat tatatgtca gactgagtc tggaagtct 
      ggtgcagta acgtagtta tataaaaga tagtgatgt 
      ttttcattt ctattgtga ttaatggag acataatgg 
      gtattcaaa tttaaacac aatgttctg taacgattt 
      gatgataca tgtcaacag ttttttatt cttgtttgt 
      atactttcg tttagttct taacgagag tcacacgtt 
      ttcttgctt tcagtgtat gtaaaattt catgtacaa 
      ttaataaat taatattag acta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY