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Gene information
General information
TC014187 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2909877..2914941
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014187
    iBeetle screen iB_06324

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014187 RA
      MVKVLAFAV TEGRVAIHC HAGLGRTGV LIACYLVYS 
      LRVSANDAI RYVRLKRPG SVQTRGQIL CVRHFAQFV 
      LPRTITYCL KENINKNKY MSEFSLVRF LHRQRVILH 
      GYEERTFKF IPKIVHCIC ERILKLCGC WIDEFSSNT 
      PLTFDFLYT KLNGNIKRY ESTYSLCST NSDSLFSNE 
      LKSPEEISS PSSPNPSEN GDPSDSFSE LSTLDGDEI 
      REELLIENR CFQELETQK CFNQEMSEE SIVIQDINV 
      AVDALLLDY STLSNEIKK KVRQFQIEI NSSQVGWAK 
      LAIETDLRI LAMLLFEWI ESLKHPVMK REFFENIVV 
      NYKQPEVCF QKFPAEEGY FMEYLLRFV SKINPISKE 
      NQEDLIKRI IAACSQQTL LIQGKMKPI DKGFKKLRD 
      GTLACTIEF FQSLFELIE VHHSLKSSV YLEESKMYN 
      ENDLENDFQ
    • >TC014187 RB
      MMYNQHRCL GRTGVLIAC YLVYSLRVS ANDAIRYVR 
      LKRPGSVQT RGQILCVRH FAQFVLPRT ITYCLKENI 
      NKNKYMSEF SLVRFLHRQ RVILHGYEE RTFKFIPKI 
      VHCICERIL KLCGCWIDE FSSNTPLTF DFLYTKLNG 
      NIKRYESTY SLCSTNSDS LFSNELKSP EEISSPSSP 
      NPSENGDPS DSFSELSTL DGDEIREEL LIENRCFQE 
      LETQKCFNQ EMSEESIVI QDINVAVDA LLLDYSTLS 
      NEIKKKVRQ FQIEINSSQ VGWAKLAIE TDLRILAML 
      LFEWIESLK HPVMKREFF ENIVVNYKQ PEVCFQKFP 
      AEEGYFMEY LLRFVSKIN PISKENQED LIKRIIAAC 
      SQQTLLIQG KMKPIDKGF KKLRDGTLA CTIEFFQSL 
      FELIEVHHS LKSSVYLEE SKMYNENDL ENDFQ
    • >TC014187 RC
      MELYEVKSA TNETVQANY SKLSDHIRR LTPQGIQCS 
      VFCGGTNCK YENPENWKA DSLAIQGIY SHWITDDIL 
      AMARPSTMV IVQKNIIQQ FESLGIKSI INLQSPREH 
      ASCGQPLEE SGFSYDPNI FMEHNIFYY NFAWKDYGD 
      ATLVGLLNM VKVLAFAVT EGRVAIHCH AGLGRTGVL 
      IACYLVYSL RVSANDAIR YVRLKRPGS VQTRGQILC 
      VRHFAQFVL PRTITYCLK ENINKNKYM SEFSLVRFL 
      HRQRVILHG YEERTFKFI PKIVHCICE RILKLCGCW 
      IDEFSSNTP LTFDFLYTK LNGNIKRYE STYSLCSTN 
      SDSLFSNEL KSPEEISSP SSPNPSENG DPSDSFSEL 
      STLDGDEIR EELLIENRC FQELETQKC FNQEMSEES 
      IVIQDINVA VDALLLDYS TLSNEIKKK VRQFQIEIN 
      SSQVGWAKL AIETDLRIL AMLLFEWIE SLKHPVMKR 
      EFFENIVVN YKQPEVCFQ KFPAEEGYF MEYLLRFVS 
      KINPISKEN QEDLIKRII AACSQQTLL IQGKMKPID 
      KGFKKLRDG TLACTIEFF QSLFELIEV HHSLKSSVY 
      LEESKMYNE NDLENDFQ
    CDS sequence
    • >TC014187 RA
      atggtgaag gtcctggcg tttgccgta acggaagga 
      cgcgtggct attcactgc cacgccggt ctagggaga 
      actggggtt ctgatagcg tgttacttg gtttactcg 
      cttcgagtt agcgcaaac gatgccata agatacgtc 
      cggttgaaa aggccgggg tcggtccaa acccgggga 
      caaatttta tgtgtccgc catttcgca caatttgta 
      cttccccga actataacc tactgtctc aaagaaaac 
      atcaacaaa aacaaatat atgagtgag tttagcttg 
      gtgcggttc ctgcacagg cagagggtc atcctccac 
      ggctacgag gaacgcact ttcaaattc ataccgaag 
      atcgtccac tgcatttgc gagcggatt ttgaagctg 
      tgtggctgc tggattgac gaattctcg tcaaacacg 
      ccactcacc ttcgatttc ctttacacg aaactcaac 
      gggaacatc aaaagatac gaaagtacc tacagcttg 
      tgcagcacg aactccgat tcgctcttt tccaacgaa 
      ttaaagtct cctgaagaa atctcttca ccttctagt 
      ccaaaccct tccgagaat ggcgacccg agcgactcg 
      ttttccgaa ttgagcacc ctagacggt gacgaaata 
      agggaagaa cttttaata gaaaatcga tgttttcaa 
      gagttggaa acacaaaag tgtttcaat caagaaatg 
      agtgaggaa tcaatagtt atccaagac ataaatgtc 
      gcagtcgat gcactatta ctggattat tcaacgcta 
      tcgaatgag atcaaaaag aaagtgaga cagtttcaa 
      atcgaaatt aattcgtcc caagtcggg tgggcgaag 
      cttgcgatt gagacggac ttgaggatt ttagcgatg 
      ctgcttttt gagtggatc gagagtctg aagcatccg 
      gtcatgaaa cgcgagttt tttgaaaac attgtcgtc 
      aattacaag caacctgaa gtctgcttc cagaagttt 
      ccagctgaa gaaggctat ttcatggaa tatttgctc 
      cgatttgtc agtaaaatt aacccgatt agtaaagag 
      aatcaggag gatttgata aaacggata attgccgca 
      tgctcgcag caaactttg ctaatccaa gggaagatg 
      aaaccgata gataaaggg tttaaaaaa ctgcgggac 
      ggaacgttg gcttgcaca atagagttt ttccagtcg 
      ttgtttgaa ctgattgaa gttcaccac agccttaag 
      tcgagtgtt tacttagaa gaaagcaag atgtataac 
      gagaatgat ctcgagaat gacttccaa taa
    • >TC014187 RB
      atgatgtat aatcaacat cgctgtcta gggagaact 
      ggggttctg atagcgtgt tacttggtt tactcgctt 
      cgagttagc gcaaacgat gccataaga tacgtccgg 
      ttgaaaagg ccggggtcg gtccaaacc cggggacaa 
      attttatgt gtccgccat ttcgcacaa tttgtactt 
      ccccgaact ataacctac tgtctcaaa gaaaacatc 
      aacaaaaac aaatatatg agtgagttt agcttggtg 
      cggttcctg cacaggcag agggtcatc ctccacggc 
      tacgaggaa cgcactttc aaattcata ccgaagatc 
      gtccactgc atttgcgag cggattttg aagctgtgt 
      ggctgctgg attgacgaa ttctcgtca aacacgcca 
      ctcaccttc gatttcctt tacacgaaa ctcaacggg 
      aacatcaaa agatacgaa agtacctac agcttgtgc 
      agcacgaac tccgattcg ctcttttcc aacgaatta 
      aagtctcct gaagaaatc tcttcacct tctagtcca 
      aacccttcc gagaatggc gacccgagc gactcgttt 
      tccgaattg agcacccta gacggtgac gaaataagg 
      gaagaactt ttaatagaa aatcgatgt tttcaagag 
      ttggaaaca caaaagtgt ttcaatcaa gaaatgagt 
      gaggaatca atagttatc caagacata aatgtcgca 
      gtcgatgca ctattactg gattattca acgctatcg 
      aatgagatc aaaaagaaa gtgagacag tttcaaatc 
      gaaattaat tcgtcccaa gtcgggtgg gcgaagctt 
      gcgattgag acggacttg aggatttta gcgatgctg 
      ctttttgag tggatcgag agtctgaag catccggtc 
      atgaaacgc gagtttttt gaaaacatt gtcgtcaat 
      tacaagcaa cctgaagtc tgcttccag aagtttcca 
      gctgaagaa ggctatttc atggaatat ttgctccga 
      tttgtcagt aaaattaac ccgattagt aaagagaat 
      caggaggat ttgataaaa cggataatt gccgcatgc 
      tcgcagcaa actttgcta atccaaggg aagatgaaa 
      ccgatagat aaagggttt aaaaaactg cgggacgga 
      acgttggct tgcacaata gagtttttc cagtcgttg 
      tttgaactg attgaagtt caccacagc cttaagtcg 
      agtgtttac ttagaagaa agcaagatg tataacgag 
      aatgatctc gagaatgac ttccaataa
    • >TC014187 RC
      atggaactg tacgaagtt aaaagtgcg actaatgaa 
      accgtgcaa gcgaactac agtaaatta tctgatcat 
      ataagacgg ttgacaccg caaggtatt cagtgtagc 
      gttttctgt ggcgggact aattgtaaa tacgaaaat 
      ccggaaaac tggaaagcc gacagtttg gccatacag 
      gggatatac tcacattgg ataactgac gatatttta 
      gcgatggca cgtcctagt actatggtg attgtacaa 
      aagaatata attcaacaa tttgaaagt ttaggaata 
      aaatcgata attaattta caatcaccg agagagcat 
      gcaagttgc gggcaaccg ctggaagaa tcgggcttt 
      tcttacgac ccaaatata ttcatggaa cacaatatt 
      ttttattac aatttcgcc tggaaggac tacggggat 
      gccacgctg gtgggcctc ctcaacatg gtgaaggtc 
      ctggcgttt gccgtaacg gaaggacgc gtggctatt 
      cactgccac gccggtcta gggagaact ggggttctg 
      atagcgtgt tacttggtt tactcgctt cgagttagc 
      gcaaacgat gccataaga tacgtccgg ttgaaaagg 
      ccggggtcg gtccaaacc cggggacaa attttatgt 
      gtccgccat ttcgcacaa tttgtactt ccccgaact 
      ataacctac tgtctcaaa gaaaacatc aacaaaaac 
      aaatatatg agtgagttt agcttggtg cggttcctg 
      cacaggcag agggtcatc ctccacggc tacgaggaa 
      cgcactttc aaattcata ccgaagatc gtccactgc 
      atttgcgag cggattttg aagctgtgt ggctgctgg 
      attgacgaa ttctcgtca aacacgcca ctcaccttc 
      gatttcctt tacacgaaa ctcaacggg aacatcaaa 
      agatacgaa agtacctac agcttgtgc agcacgaac 
      tccgattcg ctcttttcc aacgaatta aagtctcct 
      gaagaaatc tcttcacct tctagtcca aacccttcc 
      gagaatggc gacccgagc gactcgttt tccgaattg 
      agcacccta gacggtgac gaaataagg gaagaactt 
      ttaatagaa aatcgatgt tttcaagag ttggaaaca 
      caaaagtgt ttcaatcaa gaaatgagt gaggaatca 
      atagttatc caagacata aatgtcgca gtcgatgca 
      ctattactg gattattca acgctatcg aatgagatc 
      aaaaagaaa gtgagacag tttcaaatc gaaattaat 
      tcgtcccaa gtcgggtgg gcgaagctt gcgattgag 
      acggacttg aggatttta gcgatgctg ctttttgag 
      tggatcgag agtctgaag catccggtc atgaaacgc 
      gagtttttt gaaaacatt gtcgtcaat tacaagcaa 
      cctgaagtc tgcttccag aagtttcca gctgaagaa 
      ggctatttc atggaatat ttgctccga tttgtcagt 
      aaaattaac ccgattagt aaagagaat caggaggat 
      ttgataaaa cggataatt gccgcatgc tcgcagcaa 
      actttgcta atccaaggg aagatgaaa ccgatagat 
      aaagggttt aaaaaactg cgggacgga acgttggct 
      tgcacaata gagtttttc cagtcgttg tttgaactg 
      attgaagtt caccacagc cttaagtcg agtgtttac 
      ttagaagaa agcaagatg tataacgag aatgatctc 
      gagaatgac ttccaataa
    mRNA sequence
    • >TC014187 RA
      tccggaatt aaaacaccg attacaaat aatatcaca 
      tttattgct tacatggtt tacacataa atacaaata 
      acttatact taattttat aaaatcaga ctgactcga 
      acggtatgt aacatattc tgcgtacaa ttgagaggg 
      gtatatttg ggacaaaaa ttgcgataa ataggagaa 
      tagggccta atacgataa atactatgt acacgataa 
      aattccaaa gggattttg gaaaggtgt catcataca 
      ttgctatgg acagcatgc cccctgaaa aaaaaagca 
      aaaaaaaat gtataaatg cccttgtta attgtttag 
      aataaataa cgataaatg tgtcgcccc gcattacac 
      atctctgac ccactttat ccaatgaca taagtgtga 
      cataatttc gattattgt taatctaaa ttcgaaagt 
      gaattgtgc cgagtacgg taattcaca agttttgaa 
      tgaattttt aaaactacc ttggattat ttgcagagt 
      ttgttttgg ggagcactt taaacataa ttaagtgtt 
      ggtcgcagt ttgaataat tccaaagaa cgtgatcat 
      cacaacaag aaccacaca aaataactt ctcccacaa 
      agactagtt tgtacaatc tgagcaagt cggctatac 
      ctggacgac cttcgctgt tttatttaa tactttcgc 
      ttttacgcc atggatttg ttcatttct gctacaatt 
      atgtaattg taacgaacc gatttggaa tgttggttt 
      atttaatca cgtctggag tataaacaa attgagcta 
      aacgtgaga caaacgcta agtcggaat tgttcaatg 
      taagccagg gtcaggttt taattgttt cgctcatca 
      cttgcattg cgaaatcgt aatttatta aaattgatg 
      caaacaggt cttgggcaa tgttcgaat cccaaatgt 
      caaatattt actcaacgt gatgcaaaa cggtactca 
      atcaataaa aaactcaat taaatctgg tttcatgtt 
      tttattctt caagcgcca aaattccaa tttttagcg 
      cagtgcgtg tttaattgg ttgcctaca ctaacactt 
      ttttgttca aatcattat taatttatt aattaacca 
      gtagaataa ctaataaaa ttcaccttt gtttgttat 
      ttgcacaaa aaatacagc gcggtacgc aattttttg 
      aatatttct tatgacccg aacagcccc tgtttacct 
      gccccacat gactaattc aaaatcggc gaatcgtcc 
      gtatacgca gtgcgcaaa taattttta tttttcagt 
      gtttctgac gtgtcggat atttacctt agcgaccat 
      tcttcagct tttatgcag ataatatct aataataaa 
      ttcaagcat gcgctaaaa gctcgatta aaaatgtgc 
      ttgtttgat tgtatcatt ttgaaataa agcggtgag 
      ggtgtatgt agtttttta ccatggaac tgtacgaag 
      ttaaaagtg cgactaatg aaaccgtgc aagcgaact 
      acagtaaat tatctgatc atataagac ggttgacac 
      cgcaaggta ttcagtgta gcgttttct gtggcggga 
      ctaattgta aatacgaaa atccggaaa actggaaag 
      ccgacagtt tggccatac aggggatat actcacatt 
      ggataactg acgatattt tagcgatgg cacgtccta 
      gtactatgg tgattgtac aaaagaata taattcaac 
      aatttgaaa ggtacaccc gagctccta aattattta 
      gttattatg atacctctt tagtttagg aataaaatc 
      gataattaa tttacaatc accgagaga gcatgcaag 
      ttgcgggca accgctgga agaatcggg cttttctta 
      cgacccaaa tatattcat ggaacacaa tagtaagtc 
      ataacaatg ttatcaacc gcatctttt tgtgtgcat 
      gttaattgc taattcggt tgaggaatc gatccgata 
      tttacactt gaactactt ttcacctac tcaaaccca 
      taagtaaat ttattcaat aatgtgcat gagctttcg 
      ttgacataa caacataaa taccatttc ctgcacgaa 
      aaacaaaac taactgaaa ttagagata attccctcg 
      tgggcaatc gcgattaca cgaaataat tacctaaag 
      actttatct gtattgatt ccgaaaaga aaatattct 
      ataaataaa aaactgtaa ttacgtctg cgaatggaa 
      aacattgcc acaatatcg gcccccgaa aacaacaaa 
      gtataaaaa tagcgtttc cggaaaatg caaatgcga 
      aaaaccccc gtcataaat ctttcagtc agttattac 
      gcgtatgta gattttcta aatttctcg tccgttgat 
      aaggaaatc gggtcgtac aacataggg gtataaacc 
      tgataagtt aacctacat aaaatcgcg tgaattcgc 
      cccgttaac gtgtttaaa tcgaattaa tcattaatt 
      ggtgcttga gcccaactg tctatttaa atcgggcag 
      aattgattc gtaaatgaa tcgacttgt tgaataccc 
      ctatctgaa ctttattta atgaagaac cgtattcga 
      ttagggtaa attatttac gcattgttc taactataa 
      accggccga gctttggcc gaaatcgac aaacccaat 
      aagcttcca attacccga agggaatta gggttttta 
      tcccgtggg aaattcctt tcccggtca attatgctc 
      gcgtgacgt gcaaagtgc gctttgata tcgactttt 
      tccataatt aattagcct catttattt taatggggg 
      atttttagt cacactcaa gtcgcacag tttaaaatc 
      catgcccat ttccagttt tttattaca atttcgcct 
      ggaaggact acggggatg ccacgctgg tgggcctcc 
      tcaacatgg tgaaggtcc tggcgtttg ccgtaacgg 
      aaggacgcg tggctattc actgccacg ccggtctag 
      ggagaactg gggttctga tagcgtgtt acttggttt 
      actcgcttc gagttagcg caaacgatg ccataagat 
      acgtccggt tgaaaaggc cggggtcgg tccaaaccc 
      ggggacaaa ttttatgtg tccgccatt tcgcacaat 
      ttgtacttc cccgaacta taacctact gtctcaaag 
      aaaacatca acaaaaaca aatatatga gtgagttta 
      gcttggtgc ggttcctgc acaggcaga gggtcatcc 
      tccacggct acgaggaac gcactttca aattcatac 
      cgaagatcg tccactgca tttgcgagc ggattttga 
      agctgtgtg gctgctgga ttgacgaat tctcgtcaa 
      acacgccac tcaccttcg atttccttt acacgaaac 
      tcaacggga acatcaaaa gatacgaaa gtacctaca 
      gcttgtgca gcacgaact ccgattcgc tcttttcca 
      acgaattaa agtctcctg aagaaatct cttcacctt 
      ctagtccaa acccttccg agaatggcg acccgagcg 
      actcgtttt ccgaattga gcaccctag acggtgacg 
      aaataaggg aagaacttt taatagaaa atcgatgtt 
      ttcaagagt tggaaacac aaaagtgtt tcaatcaag 
      aaatgagtg aggaatcaa tagttatcc aagacataa 
      atgtcgcag tcgatgcac tattactgg attattcaa 
      cgctatcga atgagatca aaaagaaag tgagacagt 
      ttcaaatcg aaattaatt cgtcccaag tcgggtggg 
      cgaagcttg cgattgaga cggacttga ggattttag 
      cgatgctgc tttttgagt ggatcgaga gtctgaagc 
      atccggtca tgaaacgcg agttttttg aaaacattg 
      tcgtcaatt acaagcaac ctgaagtct gcttccaga 
      agtttccag ctgaagaag gctatttca tggaatatt 
      tgctccgat ttgtcagta aaattaacc cgattagta 
      aagagaatc aggaggatt tgataaaac ggataattg 
      ccgcatgct cgcagcaaa ctttgctaa tccaaggga 
      agatgaaac cgatagata aagggttta aaaaactgc 
      gggacggaa cgttggctt gcacaatag agtttttcc 
      agtcgttgt ttgaactga ttgaagttc accacagcc 
      ttaagtcga gtgtttact tagaagaaa gcaagatgt 
      ataacgaga atgatctcg agaatgact tccaataaa 
      cgaaatgtg tgtgtgaaa tactatttt tttaatgta 
      tg
    • >TC014187 RB
      tccggaatt aaaacaccg attacaaat aatatcaca 
      tttattgct tacatggtt tacacataa atacaaata 
      acttatact taattttat aaaatcaga ctgactcga 
      acggtatgt aacatattc tgcgtacaa ttgagaggg 
      gtatatttg ggacaaaaa ttgcgataa ataggagaa 
      tagggccta atacgataa atactatgt acacgataa 
      aattccaaa gggattttg gaaaggtgt catcataca 
      ttgctatgg acagcatgc cccctgaaa aaaaaagca 
      aaaaaaaat gtataaatg cccttgtta attgtttag 
      aataaataa cgataaatg tgtcgcccc gcattacac 
      atctctgac ccactttat ccaatgaca taagtgtga 
      cataatttc gattattgt taatctaaa ttcgaaagt 
      gaattgtgc cgagtacgg taattcaca agttttgaa 
      tgaattttt aaaactacc ttggattat ttgcagagt 
      ttgttttgg ggagcactt taaacataa ttaagtgtt 
      ggtcgcagt ttgaataat tccaaagaa cgtgatcat 
      cacaacaag aaccacaca aaataactt ctcccacaa 
      agactagtt tgtacaatc tgagcaagt cggctatac 
      ctggacgac cttcgctgt tttatttaa tactttcgc 
      ttttacgcc atggatttg ttcatttct gctacaatt 
      atgtaattg taacgaacc gatttggaa tgttggttt 
      atttaatca cgtctggag tataaacaa attgagcta 
      aacgtgaga caaacgcta agtcggaat tgttcaatg 
      taagccagg gtcaggttt taattgttt cgctcatca 
      cttgcattg cgaaatcgt aatttatta aaattgatg 
      caaacaggt cttgggcaa tgttcgaat cccaaatgt 
      caaatattt actcaacgt gatgcaaaa cggtactca 
      atcaataaa aaactcaat taaatctgg tttcatgtt 
      tttattctt caagcgcca aaattccaa tttttagcg 
      cagtgcgtg tttaattgg ttgcctaca ctaacactt 
      ttttgttca aatcattat taatttatt aattaacca 
      gtagaataa ctaataaaa ttcaccttt gtttgttat 
      ttgcacaaa aaatacagc gcggtacgc aattttttg 
      aatatttct tatgacccg aacagcccc tgtttacct 
      gccccacat gactaattc aaaatcggc gaatcgtcc 
      gtatacgca gtgcgcaaa taattttta tttttcagt 
      gtttctgac gtgtcggat atttacctt agcgaccat 
      tcttcagct tttatgcag ataatatct aataataaa 
      ttcaagcat gcgctaaaa gctcgatta aaaatgtgc 
      ttgtttgat tgtatcatt ttgaaataa agcggtgag 
      ggtgtatgt agtttttta ccatggaac tgtacgaag 
      ttaaaagtg cgactaatg aaaccgtgc aagcgaact 
      acagtaaat tatctgatc atataagac ggttgacac 
      cgcaaggta ttcagtgta gcgttttct gtggcggga 
      ctaattgta aatacgaaa atccggaaa actggaaag 
      ccgacagtt tggccatac aggggatat actcacatt 
      ggataactg acgatattt tagcgatgg cacgtccta 
      gtactatgg tgattgtac aaaagaata taattcaac 
      aatttgaaa ggtacaccc gagctccta aattattta 
      gttattatg atacctctt tagtttagg aataaaatc 
      gataattaa tttacaatc accgagaga gcatgcaag 
      ttgcgggca accgctgga agaatcggg cttttctta 
      cgacccaaa tatattcat ggaacacaa tagtaagtc 
      ataacaatg ttatcaacc gcatctttt tgtgtgcat 
      gttaattgc taattcggt tgaggaatc gatccgata 
      tttacactt gaactactt ttcacctac tcaaaccca 
      taagtaaat ttattcaat aatgtgcat gagctttcg 
      ttgacataa caacataaa taccatttc ctgcacgaa 
      aaacaaaac taactgaaa ttagagata attccctcg 
      tgggcaatc gcgattaca cgaaataat tacctaaag 
      actttatct gtattgatt ccgaaaaga aaatattct 
      ataaataaa aaactgtaa ttacgtctg cgaatggaa 
      aacattgcc acaatatcg gcccccgaa aacaacaaa 
      gtataaaaa tagcgtttc cggaaaatg caaatgcga 
      aaaaccccc gtcataaat ctttcagtc agttattac 
      gcgtatgta gattttcta aatttctcg tccgttgat 
      aaggaaatc gggtcgtac aacataggg gtataaacc 
      tgataagtt aacctacat aaaatcgcg tgaattcgc 
      cccgttaac gtgtttaaa tcgaattaa tcattaatt 
      ggtgcttga gcccaactg tctatttaa atcgggcag 
      aattgattc gtaaatgaa tcgacttgt tgaataccc 
      ctatctgaa ctttattta atgaagaac cgtattcga 
      ttagggtaa attatttac gcattgttc taactataa 
      accggccga gctttggcc gaaatcgac aaacccaat 
      aagcttcca attacccga agggaatta gggttttta 
      tcccgtggg aaattcctt tcccggtca attatgctc 
      gcgtgacgt gcaaagtgc gctttgata tcgactttt 
      tccataatt aattagcct catttattt taatggggg 
      atttttagt cacactcaa gtcgcacag tttaaaatc 
      catgcccat ttccagttt tttattaca atttcgcct 
      ggaaggact acggggatg ccacgctgg tgggcctcc 
      tcaacatgg tgaaggtcc tggcgtttg ccgtaacgg 
      aaggacgcg tggctattc actgccacg ccggtaaga 
      atataagca acatgcgat atgaccgag ttgatgagt 
      ttgttactt ttatttctg tatttggta ttgatttta 
      ttaagtagt gtaaacacg ccgtcaatc atatttatt 
      ggtccaaca ccacgttca ttttttatt aattgctat 
      caagaaaac tttattttc tgtgttgac atgatgtat 
      aatcaacat cgctgtcta gggagaact ggggttctg 
      atagcgtgt tacttggtt tactcgctt cgagttagc 
      gcaaacgat gccataaga tacgtccgg ttgaaaagg 
      ccggggtcg gtccaaacc cggggacaa attttatgt 
      gtccgccat ttcgcacaa tttgtactt ccccgaact 
      ataacctac tgtctcaaa gaaaacatc aacaaaaac 
      aaatatatg agtgagttt agcttggtg cggttcctg 
      cacaggcag agggtcatc ctccacggc tacgaggaa 
      cgcactttc aaattcata ccgaagatc gtccactgc 
      atttgcgag cggattttg aagctgtgt ggctgctgg 
      attgacgaa ttctcgtca aacacgcca ctcaccttc 
      gatttcctt tacacgaaa ctcaacggg aacatcaaa 
      agatacgaa agtacctac agcttgtgc agcacgaac 
      tccgattcg ctcttttcc aacgaatta aagtctcct 
      gaagaaatc tcttcacct tctagtcca aacccttcc 
      gagaatggc gacccgagc gactcgttt tccgaattg 
      agcacccta gacggtgac gaaataagg gaagaactt 
      ttaatagaa aatcgatgt tttcaagag ttggaaaca 
      caaaagtgt ttcaatcaa gaaatgagt gaggaatca 
      atagttatc caagacata aatgtcgca gtcgatgca 
      ctattactg gattattca acgctatcg aatgagatc 
      aaaaagaaa gtgagacag tttcaaatc gaaattaat 
      tcgtcccaa gtcgggtgg gcgaagctt gcgattgag 
      acggacttg aggatttta gcgatgctg ctttttgag 
      tggatcgag agtctgaag catccggtc atgaaacgc 
      gagtttttt gaaaacatt gtcgtcaat tacaagcaa 
      cctgaagtc tgcttccag aagtttcca gctgaagaa 
      ggctatttc atggaatat ttgctccga tttgtcagt 
      aaaattaac ccgattagt aaagagaat caggaggat 
      ttgataaaa cggataatt gccgcatgc tcgcagcaa 
      actttgcta atccaaggg aagatgaaa ccgatagat 
      aaagggttt aaaaaactg cgggacgga acgttggct 
      tgcacaata gagtttttc cagtcgttg tttgaactg 
      attgaagtt caccacagc cttaagtcg agtgtttac 
      ttagaagaa agcaagatg tataacgag aatgatctc 
      gagaatgac ttccaataa acgaaatgt gtgtgtgaa 
      atactattt ttttaatgt atg
    • >TC014187 RC
      cttcagtca aataacaaa aataccaaa aaacaacga 
      acacaattg tccggaatt aaaacaccg attacaaat 
      aatatcaca tttattgct tacatggtt tacacataa 
      atacaaata acttatact taattttat aaaatcaga 
      ctgactcga acggtatgt aacatattc tgcgtacaa 
      ttgagaggg gtatatttg ggacaaaaa ttgcgataa 
      ataggagaa tagggccta atacgataa atactatgt 
      acacgataa aattccaaa gggattttg gaaaggtgt 
      catcataca ttgctatgg acagcatgc cccctgaaa 
      aaaaaagca aaaaaaaat gtataaatg cccttgtta 
      attgtttag aataaataa cgataaatg tgtcgcccc 
      gcattacac atctctgac ccactttat ccaatgaca 
      taagtgtga cataatttc gattattgt taatctaaa 
      ttcgaaagt gaattgtgc cgagtacgg taattcaca 
      agttttgaa tgaattttt aaaactacc ttggattat 
      ttgcagagt ttgttttgg ggagcactt taaacataa 
      ttaagtgtt ggtcgcagt ttgaataat tccaaagaa 
      cgtgatcat cacaacaag aaccacaca aaataactt 
      ctcccacaa agactagtt tgtacaatc tgagcaagt 
      cggctatac ctggacgac cttcgctgt tttatttaa 
      tactttcgc ttttacgcc atggatttg ttcatttct 
      gctacaatt atgtaattg taacgaacc gatttggaa 
      tgttggttt atttaatca cgtctggag tataaacaa 
      attgagcta aacgtgaga caaacgcta agtcggaat 
      tgttcaatg taagccagg gtcaggttt taattgttt 
      cgctcatca cttgcattg cgaaatcgt aatttatta 
      aaattgatg caaacaggt cttgggcaa tgttcgaat 
      cccaaatgt caaatattt actcaacgt gatgcaaaa 
      cggtactca atcaataaa aaactcaat taaatctgg 
      tttcatgtt tttattctt caagcgcca aaattccaa 
      tttttagcg cagtgcgtg tttaattgg ttgcctaca 
      ctaacactt ttttgttca aatcattat taatttatt 
      aattaacca gtagaataa ctaataaaa ttcaccttt 
      gtttgttat ttgcacaaa aaatacagc gcggtacgc 
      aattttttg aatatttct tatgacccg aacagcccc 
      tgtttacct gccccacat gactaattc aaaatcggc 
      gaatcgtcc gtatacgca gtgcgcaaa taattttta 
      tttttcagt gtttctgac gtgtcggat atttacctt 
      agcgaccat tcttcagct tttatgcag ataatatct 
      aataataaa ttcaagcat gcgctaaaa gctcgatta 
      aaaatgtgc ttgtttgat tgtatcatt ttgaaataa 
      agcggtgag ggtgtatgt agtttttta ccatggaac 
      tgtacgaag ttaaaagtg cgactaatg aaaccgtgc 
      aagcgaact acagtaaat tatctgatc atataagac 
      ggttgacac cgcaaggta ttcagtgta gcgttttct 
      gtggcggga ctaattgta aatacgaaa atccggaaa 
      actggaaag ccgacagtt tggccatac aggggatat 
      actcacatt ggataactg acgatattt tagcgatgg 
      cacgtccta gtactatgg tgattgtac aaaagaata 
      taattcaac aatttgaaa gtttaggaa taaaatcga 
      taattaatt tacaatcac cgagagagc atgcaagtt 
      gcgggcaac cgctggaag aatcgggct tttcttacg 
      acccaaata tattcatgg aacacaata ttttttatt 
      acaatttcg cctggaagg actacgggg atgccacgc 
      tggtgggcc tcctcaaca tggtgaagg tcctggcgt 
      ttgccgtaa cggaaggac gcgtggcta ttcactgcc 
      acgccggtc tagggagaa ctggggttc tgatagcgt 
      gttacttgg tttactcgc ttcgagtta gcgcaaacg 
      atgccataa gatacgtcc ggttgaaaa ggccggggt 
      cggtccaaa cccggggac aaattttat gtgtccgcc 
      atttcgcac aatttgtac ttccccgaa ctataacct 
      actgtctca aagaaaaca tcaacaaaa acaaatata 
      tgagtgagt ttagcttgg tgcggttcc tgcacaggc 
      agagggtca tcctccacg gctacgagg aacgcactt 
      tcaaattca taccgaaga tcgtccact gcatttgcg 
      agcggattt tgaagctgt gtggctgct ggattgacg 
      aattctcgt caaacacgc cactcacct tcgatttcc 
      tttacacga aactcaacg ggaacatca aaagatacg 
      aaagtacct acagcttgt gcagcacga actccgatt 
      cgctctttt ccaacgaat taaagtctc ctgaagaaa 
      tctcttcac cttctagtc caaaccctt ccgagaatg 
      gcgacccga gcgactcgt tttccgaat tgagcaccc 
      tagacggtg acgaaataa gggaagaac ttttaatag 
      aaaatcgat gttttcaag agttggaaa cacaaaagt 
      gtttcaatc aagaaatga gtgaggaat caatagtta 
      tccaagaca taaatgtcg cagtcgatg cactattac 
      tggattatt caacgctat cgaatgaga tcaaaaaga 
      aagtgagac agtttcaaa tcgaaatta attcgtccc 
      aagtcgggt gggcgaagc ttgcgattg agacggact 
      tgaggattt tagcgatgc tgctttttg agtggatcg 
      agagtctga agcatccgg tcatgaaac gcgagtttt 
      ttgaaaaca ttgtcgtca attacaagc aacctgaag 
      tctgcttcc agaagtttc cagctgaag aaggctatt 
      tcatggaat atttgctcc gatttgtca gtaaaatta 
      acccgatta gtaaagaga atcaggagg atttgataa 
      aacggataa ttgccgcat gctcgcagc aaactttgc 
      taatccaag ggaagatga aaccgatag ataaagggt 
      ttaaaaaac tgcgggacg gaacgttgg cttgcacaa 
      tagagtttt tccagtcgt tgtttgaac tgattgaag 
      ttcaccaca gccttaagt cgagtgttt acttagaag 
      aaagcaaga tgtataacg agaatgatc tcgagaatg 
      acttccaat aaacgaaat gtgtgtgtg aaatactat 
      ttttttaat gtatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY