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Gene information
General information
TC014174 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2789416..2791180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014174
    iBeetle screen iB_06323

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    Pupal morphology
    elytra partiallyblistered - Number of animals showing the phenotype: 7

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014174 RA
      MSFFIKSKS INGTAKFSA DKRRKRRAE PASSKNEEV 
      TSSEDEDSD HNLAEESED EHETAQEKK LRLAKVYLE 
      EIEREEQRR LQDKEVSKE VISERLKTD HLKQIGKYR 
      ITIADKYQG VDQSNYKVL KCREQQNTI TCLCLSSDD 
      NWVFSGSKD GIVVKWSLK ENKKFGIIP FVKVNVEQV 
      KGHTGCIYS IAISTDSKF LVVGDSSNL IQVWDPQTL 
      QHVSTLKGH KKPVTALCL KHNSHTLYS ASSDRSVRV 
      WTLDEMAFV ETLFGHQDG ITSIDVLNS DKPLSSGGR 
      DGTVRLWKV SEESQLIFN AHPGNIDCV KWISDQYFV 
      SGGDDGQVS VWSCLKKKP ISCVKAAHG VDSSNNQPF 
      WISAVGSHV HSDVIASGS QDGFLRLWK LENKFLQIT 
      KLFEIPVPG FINALAFTS DGRKLVVGV GRDHRLGRW 
      NTVKSAVNS IHVIPLQIN D
    CDS sequence
    • >TC014174 RA
      atgtcattt tttatcaag tcaaagtca attaacggc 
      actgctaaa ttttcagca gataaacgc agaaaacgg 
      agggctgaa cctgcctcc agtaaaaac gaggaagtg 
      accagtagc gaggacgaa gacagtgac cataatcta 
      gccgaagag tcggaagac gagcatgag acggcacaa 
      gaaaagaag ctaaggcta gccaaagtt tacttagag 
      gaaattgaa cgcgaagag caaaggcgg ttacaagac 
      aaggaagtt agtaaagaa gttatatca gaaaggcta 
      aaaacagat catttgaaa caaatcggg aagtacaga 
      attacaatt gccgacaag tatcaaggt gtagaccaa 
      agtaattat aaagttttg aaatgtcgc gagcaacaa 
      aatacaatt acgtgcctc tgtttatcg tcggacgat 
      aactgggtt ttttccggc agcaaagac ggaattgtg 
      gttaaatgg tcccttaaa gaaaataaa aaatttgga 
      attatcccg tttgttaag gtaaacgtc gaacaagtc 
      aaaggccac acgggctgc atctacagc atagcaatt 
      tccacagac agcaaattt ctagttgtt ggcgattct 
      tcgaattta attcaagtg tgggacccc caaaccctg 
      cagcatgtc tcaaccctc aaaggccac aaaaagcca 
      gtcactgcc ctttgcttg aaacacaac agtcacacc 
      ttgtactca gccagtagc gacagatct gtgagagtt 
      tggactttg gacgaaatg gcctttgtt gagacgctg 
      ttcggtcac caagacggc atcacttcc attgacgtc 
      ctcaactcc gataaaccc ctctcaagc gggggccgc 
      gacggcact gtgagactg tggaaagtt agcgaagag 
      tcccagttg atttttaac gcccatccc gggaacatc 
      gactgtgtt aaatggatc agtgaccag tattttgtc 
      tctgggggg gacgatggg caagtgagt gtttggagt 
      tgcttgaaa aagaagccc atctcatgt gttaaagcc 
      gcacatggt gttgactcg agcaataac cagccgttt 
      tggataagt gccgtgggg tcacacgtc cacagcgat 
      gtcatagcg tcagggtca caggatggc ttccttagg 
      ctttggaaa ttggaaaat aagtttctc caaataact 
      aagttgttt gaaatacca gtccctggt tttatcaac 
      gcgttggcg ttcacttca gatgggcgg aaactggtg 
      gtcggggtc ggaagggac cacaggtta ggtaggtgg 
      aacaccgtt aagtcggca gtaaacagc atacacgta 
      ataccgttg caaataaac gattaa
    mRNA sequence
    • >TC014174 RA
      tctctctta ttctatgag catgactta acctccaat 
      tcaaaaaat ttatcaacc cagcacgtg tgacaatta 
      tttacagaa tctttatag tttattacg tttacgatg 
      tcatttttt atcaagtca aagtcaatt aacggcact 
      gctaaattt tcagcagat aaacgcaga aaacggagg 
      gctgaacct gcctccagt aaaaacgag gaagtgacc 
      agtagcgag gacgaagac agtgaccat aatctagcc 
      gaagagtcg gaagacgag catgagacg gcacaagaa 
      aagaagcta aggctagcc aaagtttac ttagaggaa 
      attgaacgc gaagagcaa aggcggtta caagacaag 
      gaagttagt aaagaagtt atatcagaa aggctaaaa 
      acagatcat ttgaaacaa atcgggaag tacagaatt 
      acaattgcc gacaagtat caaggtgta gaccaaagt 
      aattataaa gttttgaaa tgtcgcgag caacaaaat 
      acaattacg tgcctctgt ttatcgtcg gacgataac 
      tgggttttt tccggcagc aaagacgga attgtggtt 
      aaatggtcc cttaaagaa aataaaaaa tttggaatt 
      atcccgttt gttaaggta aacgtcgaa caagtcaaa 
      ggccacacg ggctgcatc tacagcata gcaatttcc 
      acagacagc aaatttcta gttgttggc gattcttcg 
      aatttaatt caagtgtgg gacccccaa accctgcag 
      catgtctca accctcaaa ggccacaaa aagccagtc 
      actgccctt tgcttgaaa cacaacagt cacaccttg 
      tactcagcc agtagcgac agatctgtg agagtttgg 
      actttggac gaaatggcc tttgttgag acgctgttc 
      ggtcaccaa gacggcatc acttccatt gacgtcctc 
      aactccgat aaacccctc tcaagcggg ggccgcgac 
      ggcactgtg agactgtgg aaagttagc gaagagtcc 
      cagttgatt tttaacgcc catcccggg aacatcgac 
      tgtgttaaa tggatcagt gaccagtat tttgtctct 
      gggggggac gatgggcaa gtgagtgtt tggagttgc 
      ttgaaaaag aagcccatc tcatgtgtt aaagccgca 
      catggtgtt gactcgagc aataaccag ccgttttgg 
      ataagtgcc gtggggtca cacgtccac agcgatgtc 
      atagcgtca gggtcacag gatggcttc cttaggctt 
      tggaaattg gaaaataag tttctccaa ataactaag 
      ttgtttgaa ataccagtc cctggtttt atcaacgcg 
      ttggcgttc acttcagat gggcggaaa ctggtggtc 
      ggggtcgga agggaccac aggttaggt aggtggaac 
      accgttaag tcggcagta aacagcata cacgtaata 
      ccgttgcaa ataaacgat taatttata tctacgata 
      ttttattga aataga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY