Status
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Gene information
General information
TC014079 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5410120..5412498
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014079
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06320

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014079 RA
      MEESSLFSL EDCDKIVRK YLKSDNIQT VGFTVLSLK 
      NQNYTLKIT VVDESREKT LKFFVKSTQ CLPKVNKIL 
      FEDFKKTLP DFDAKITPK LYYDKSDLL LFEDLTSQG 
      FKSHKSGSF DLKHLRIVV KTLAKLHAA SLAFEEIKS 
      ARLDEENKL NRASLDLKN PEVTKLLAS LLDEEKLSS 
      VITDLEERQ LSPNFRRVL SHNNLTTKN IMFKYRDNV 
      PVEFCDFLQ VIFFNTDQD FRQYYFESL ISFYHGCLK 
      EQFEHYLLN IDKVLPFED LRLETHYYL SLIKLEAAL 
      KNPTPTTLE ELREILNCP ILSREDCYT IIRNKIGSS 
      DYKFEFFQL TPLDTVNGF LGNYHKLKL KISGETINC 
      FIKCMPKSK VSKDILQET GCFSKEIFI YEILIPQML 
      KLGIATINE CLPTCYFQR SDDLMVFDD LSFNYRTLK 
      AQVPFDFPL LELAIKKLA KFHASMFVF EEKMLFRLD 
      EKYGDYLED IFFWKEKTH AGAVLMQTG LRSITDILD 
      LFPEEKSET NVKNFEKCW PKLQELFYT VTKPSSCYR 
      NVLNHGDLW TNNIMVRFD GTKPIDCQF IDYQCTRYC 
      PPAHDYLSV VHLTTDKAN RRKHEEEIR SIYYTELGK 
      VLEAYNYDV RKILPFEEF CQTIDYIKP QMVLQTMIY 
      SCFCISTPE EIASFLTNE ETSTRTFFH DRKDYLAEM 
      YKKSESFRI RLRECVLDL YELCSSMVP LN
    CDS sequence
    • >TC014079 RA
      atggaagaa tcgagtttg ttttcatta gaagattgt 
      gacaaaatc gttcgaaag tacctcaaa agtgacaat 
      atccagact gttggtttt accgtgctc tccctcaaa 
      aaccaaaat tatacactc aaaatcacc gtcgttgat 
      gaatcgaga gaaaaaact ctaaaattt ttcgttaaa 
      tcaacgcaa tgtttaccg aaagtcaac aaaatcctc 
      tttgaagat ttcaagaaa acgctgccg gactttgat 
      gcaaaaatc accccaaaa ctctattac gacaagtcc 
      gacttgttg cttttcgaa gacttgaca tcgcaaggg 
      ttcaagagt cacaaaagt gggagtttt gatttaaaa 
      catcttaga attgtggtg aagactttg gcaaaatta 
      catgcagca agtcttgct tttgaagaa ataaaatca 
      gcgagactg gatgaagaa aacaaatta aatcgtgcg 
      agtctcgat ttgaagaat cccgaggta acaaaactt 
      cttgcttct ttgttggat gaagaaaaa ctgtcgagc 
      gttatcaca gatttggaa gaacgccag ttatcacca 
      aactttcga agagtccta agtcacaat aacttgacc 
      acgaagaat attatgttc aaatatcgc gacaatgtt 
      ccagtagaa ttttgcgac tttcttcaa gtaattttc 
      ttcaacacg gatcaagac ttccgccaa tattatttc 
      gaatcttta atttcgttt taccacggt tgtttgaaa 
      gaacagttc gaacattat cttctcaac attgacaag 
      gtgttgcct tttgaagat ttacgttta gagactcac 
      tattacttg tctttgatt aaactggaa gcagctttg 
      aaaaatcct actccgacg actttagaa gaattgagg 
      gaaatccta aattgtcca attctgtcc cgagaagat 
      tgctacaca ataatcagg aataaaatt ggaagttct 
      gattacaaa tttgaattt ttccaacta acaccgcta 
      gatacagtc aatggattt ttgggcaat taccacaaa 
      ctcaaactc aaaatttct ggcgaaaca atcaactgt 
      ttcatcaaa tgtatgcca aaaagtaaa gtttcgaaa 
      gacatattg caagaaact ggatgcttc tccaaggaa 
      attttcatt tacgaaatt ctcatcccc caaatgttg 
      aagctcgga attgcaaca atcaacgaa tgtctcccg 
      acttgttac ttccaacgg agtgacgat cttatggtt 
      tttgacgac ttgtctttc aattatcgc acgcttaaa 
      gcccaagtc cccttcgat tttccttta cttgaacta 
      gcaataaaa aaacttgca aagtttcac gccagtatg 
      ttcgtcttt gaggaaaaa atgttgttc cgtctggac 
      gaaaagtac ggtgattat ttagaagat atatttttt 
      tggaaagag aagacacac gctggtgct gttttgatg 
      caaactggc cttaggtca attaccgac attctggat 
      ttattccca gaggaaaaa agcgagaca aacgtgaaa 
      aattttgag aagtgttgg cccaaatta caggagttg 
      ttttataca gttacaaag ccttcgagt tgttaccga 
      aatgtgcta aaccatggt gatttatgg actaacaac 
      atcatggta cggtttgat ggtacgaaa ccaatcgat 
      tgccagttt atcgactat cagtgtacc agatattgc 
      ccaccggcc catgattac ctttctgtg gttcaccta 
      accactgac aaggccaac aggaggaaa catgaggag 
      gaaataagg tcgatttat tacaccgaa ctggggaaa 
      gttttggag gcgtacaat tacgacgtc aggaaaatt 
      cttcctttt gaagaattt tgccaaact attgattac 
      atcaagcca caaatggtg ctacagact atgatttac 
      tcctgtttt tgcataagt acgccggaa gaaattgct 
      tcgtttctg accaacgag gagacttca acacggacg 
      tttttccac gatcggaag gactacttg gccgaaatg 
      tacaaaaaa tccgagtct ttcagaatc cgacttcga 
      gagtgtgtg ttggatttg tacgagttg tgctcctcc 
      atggtgcca ttgaactga
    mRNA sequence
    • >TC014079 RA
      ctcgtgata taaaaccgg aattagaac aaactctcc 
      cagtaccaa aaatggaag aatcgagtt tgttttcat 
      tagaagatt gtgacaaaa tcgttcgaa agtacctca 
      aaagtgaca atatccaga ctgttggtt ttaccgtgc 
      tctccctca aaaaccaaa attatacac tcaaaatca 
      ccgtcgttg atgaatcga gagaaaaaa ctctaaaat 
      ttttcgtta aatcaacgc aatgtttac cgaaagtca 
      acaaaatcc tctttgaag atttcaaga aaacgctgc 
      cggactttg atgcaaaaa tcaccccaa aactctatt 
      acgacaagt ccgacttgt tgcttttcg aagacttga 
      catcgcaag ggttcaaga gtcacaaaa gtgggagtt 
      ttgatttaa aacatctta gaattgtgg tgaagactt 
      tggcaaaat tacatgcag caagtcttg cttttgaag 
      aaataaaat cagcgagac tggatgaag aaaacaaat 
      taaatcgtg cgagtctcg atttgaaga atcccgagg 
      taacaaaac ttcttgctt ctttgttgg atgaagaaa 
      aactgtcga gcgttatca cagatttgg aagaacgcc 
      agttatcac caaactttc gaagagtcc taagtcaca 
      ataacttga ccacgaaga atattatgt tcaaatatc 
      gcgacaatg ttccagtag aattttgcg actttcttc 
      aagtaattt tcttcaaca cggatcaag acttccgcc 
      aatattatt tcgaatctt taatttcgt tttaccacg 
      gttgtttga aagaacagt tcgaacatt atcttctca 
      acattgaca aggtgttgc cttttgaag atttacgtt 
      tagagactc actattact tgtctttga ttaaactgg 
      aagcagctt tgaaaaatc ctactccga cgactttag 
      aagaattga gggaaatcc taaattgtc caattctgt 
      cccgagaag attgctaca caataatca ggaataaaa 
      ttggaagtt ctgattaca aatttgaat ttttccaac 
      taacaccgc tagatacag tcaatggat ttttgggca 
      attaccaca aactcaaac tcaaaattt ctggcgaaa 
      caatcaact gtttcatca aatgtatgc caaaaagta 
      aagtttcga aagacatat tgcaagaaa ctggatgct 
      tctccaagg aaattttca tttacgaaa ttctcatcc 
      cccaaatgt tgaagctcg gaattgcaa caatcaacg 
      aatgtctcc cgacttgtt acttccaac ggagtgacg 
      atcttatgg tttttgacg acttgtctt tcaattatc 
      gcacgctta aagcccaag tccccttcg attttcctt 
      tacttgaac tagcaataa aaaaacttg caaagtttc 
      acgccagta tgttcgtct ttgaggaaa aaatgttgt 
      tccgtctgg acgaaaagt acggtgatt atttagaag 
      atatatttt tttggaaag agaagacac acgctggtg 
      ctgttttga tgcaaactg gccttaggt caattaccg 
      acattctgg atttattcc cagaggaaa aaagcgaga 
      caaacgtga aaaattttg agaagtgtt ggcccaaat 
      tacaggagt tgttttata cagttacaa agccttcga 
      gttgttacc gaaatgtgc taaaccatg gtgatttat 
      ggactaaca acatcatgg tacggtttg atggtacga 
      aaccaatcg attgccagt ttatcgact atcagtgta 
      ccagatatt gcccaccgg cccatgatt acctttctg 
      tggttcacc taaccactg acaaggcca acaggagga 
      aacatgagg aggaaataa ggtcgattt attacaccg 
      aactgggga aagttttgg aggcgtaca attacgacg 
      tcaggaaaa ttcttcctt ttgaagaat tttgccaaa 
      ctattgatt acatcaagc cacaaatgg tgctacaga 
      ctatgattt actcctgtt tttgcataa gtacgccgg 
      aagaaattg cttcgtttc tgaccaacg aggagactt 
      caacacgga cgtttttcc acgatcgga aggactact 
      tggccgaaa tgtacaaaa aatccgagt ctttcagaa 
      tccgacttc gagagtgtg tgttggatt tgtacgagt 
      tgtgctcct ccatggtgc cattgaact gaattacaa 
      taaccttga tggcaaaac gtgtgatta tttttatct 
      cggtgtttc tcccgacct tgataccag gcgaaaaaa 
      ataaataaa accaagcaa accacttgt aatacctct 
      aattactgt tatgaataa aaataagtt caaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY