GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06315 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)| Around 60 larvae on slide, most hatched. Don't show any obvious defects. |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC013773 RA
MYLIAIVIV LISLYLIVK PVKKTGNID DFPEPPSKV FFGHALDAK STTEILNVT TNYVKKYGK IVKLRLGPF FKALVVTDY KLVEFFLSS NSLLTKSQN YAFARSWLG NGLILANGT HWKQHRKIL TPAFHLEIL REYLETFDS VGRIFIEKL KSLDNVQSV DLDALVPLC TLDVICETA MGTKINAQK GENSEYVSS VKEMCRIIV ARFLSPLKI LKCTYWMTH DFYTERKVV NILHSFTSK VIDGRKTNR NETEKSGKK KAFLDLLLK FSDIEKLSD RDIREEVDT FMFAGHDTT ATGVCFALY LLANNPEVQ EKVLSEQKE LFGDEKNPC VTYQELQNM KYLEYVIKE TLRLYPSVP VIGRYLKED TTFGDRVIS AKTNVAIFI YGIHRNPDY FPEPEKFIP ERFENMTNL PPYAYIPFS AGPRNCIGQ KFAMLEMKS LISKVIRHF ELTPANPHH ELVLAAETV LKSANGIKI GLRARSENK M
- >TC013773 RA
atgtactta attgcgatc gttattgtt ttgatttca ctgtattta atcgtaaaa ccagtgaaa aaaactgga aacatcgat gatttccct gaacccccg tcgaaggta tttttcgga catgccttg gacgccaaa tcaaccaca gaaattcta aatgtaacg actaactac gtaaaaaaa tatgggaaa atcgtcaag ctcaggttg gggcctttc tttaaggca cttgtggta acagactac aaactagtc gagtttttt ctaagcagc aattccctg ttaaccaaa tcacaaaat tatgcattt gcaaggtcg tggcttgga aatggatta attttagcc aacggaacg cactggaag caacaccgg aaaatcctc acacctgcg tttcatctc gaaattttg agagaatat ctcgaaact tttgattca gttgggagg atttttata gaaaaatta aaaagtctg gataatgtg caaagtgtg gacttggac gctttggtc ccactgtgc accctagac gtaatatgt gaaacggca atgggcacc aaaatcaac gcccaaaaa ggcgaaaac tcggaatat gtgagcagc gtgaaggaa atgtgtcgg atcatagtc gcgcgattt ttgtcacct ttgaaaata ttgaaatgt acttattgg atgacgcac gatttctac actgagcgc aaagtcgta aacattttg cacagtttt acttctaaa gtcatcgac ggcagaaaa acgaatcgc aatgaaacg gaaaaaagt gggaaaaaa aaggcattt ttagacttg ctgttgaag ttttcagat attgaaaaa ttgtctgat cgtgatatt cgtgaggaa gtggacact tttatgttt gcgggtcat gataccacc gcaactggt gtttgtttc gctttgtat cttctggcc aacaatccg gaagttcag gaaaaagta ttaagtgaa caaaaagaa ttgtttggg gacgaaaaa aatccttgt gtgacttat caagagtta caaaatatg aaatatttg gagtacgtc atcaaggaa acacttaga ctgtacccc tcagtgcca gtcattgga agatacttg aaagaagat acaacattt ggggatcgg gtgatttcc gcaaaaaca aacgttgcc atatttatc tacggtatt catcgaaat cccgattat tttccggaa ccggaaaag tttattcca gaaagattc gaaaacatg accaatttg cccccttat gcctacata ccgtttagt gctggaccc agaaattgc ataggccaa aaatttgcc atgttggaa atgaaaagt ctgatttcg aaggtgatc cgccatttt gaattaacc ccagccaat ccacaccac gagttggtt ttagctgct gaaaccgtc ctaaagtca gccaatggt attaaaatt ggacttaga gcccgctcg gaaaataaa atgtga
- >TC013773 RA
tggtccgaa attcaggga attagaagc aaagaattg taaaaaaca aggttggat tcatcatta agtaaaatt cttccacaa ccagctttt acctgacga catccttcc tcgcaaaat ttaaccact ctcacgtta agactatga acctcttac aattcaatt tttgggtcc ttttgttga caaaccatt gttgctaaa agttgaaga aaacaatct tgtacaaaa gttgtggtt cgatttttg cttttacca cgtcttcga ataaaatca cctgtattt ctatatctt taaatcatt cttgaaatg gatttatgc gagatacca aacctctga gctcttctg tgataacat cattctttt ttatgtaat ttaacctct taaatacgt tgatttcaa atgttcttg ttttcttgc ttgtcgtgg catattgtt taaatagac ttgaccact aagccccct tgaaaaact gtgaataaa ggcgtttga aatgtgatc gttatttta ccttttcat tttcttgct gcagaagat aatggaaca cagaatttt cgattaaat agtgaccat ctgacgaaa ttatttgat aatacacta ttaaagagc aaattaagt gatattcgt gatgtttta ggttatgtt attagtaac aaattatca gtaacaaaa aaaaaacag tattgccac ctgaaacag taattctta atcacctcg tatttacac atgtttatt tactagttt gatggtttt tactatttt tggatcgaa catgaaaag tgcatcaca agaagacct tgatcttta aataacagc cttattgtt gaactttac tctaatttc catcaactt agaatgcat tcaagcgtt taatcttat ctaactctt ctatacaaa catttttaa cgcgattgc aaacatgta cttaattgc gatcgttat tgttttgat ttcactgta tttaatcgt aaaaccagt gaaaaaaac tggaaacat cgatgattt ccctgaacc cccgtcgaa ggtattttt cggacatgc cttggacgc caaatcaac cacagaaat tctaaatgt aacgactaa ctacgtaaa aaaatatgg gaaaatcgt caagctcag gttggggcc tttctttaa ggcacttgt ggtaacaga ctacaaact agtcgagtt ttttctaag cagcaattc cctgttaac caaatcaca aaattatgc atttgcaag gtcgtggct tggaaatgg attaatttt agccaacgg aacgcactg gaagcaaca ccggaaaat cctcacacc tgcgtttca tctcgaaat tttgagaga atatctcga aacttttga ttcagttgg gaggatttt tatagaaaa attaaaaag tctggataa tgtgcaaag tgtggactt ggacgcttt ggtcccact gtgcaccct agacgtaat atgtgaaac ggcaatggg caccaaaat caacgccca aaaaggcga aaactcgga atatgtgag cagcgtgaa ggaaatgtg tcggatcat agtcgcgcg atttttgtc acctttgaa aatattgaa atgtactta ttggatgac gcacgattt ctacactga gcgcaaagt cgtaaacat tttgcacag ttttacttc taaagtcat cgacggcag aaaaacgaa tcgcaatga aacggaaaa aagtgggaa aaaaaaggc atttttaga cttgctgtt gaagttttc agatattga aaaattgtc tgatcgtga tattcgtga ggaagtgga cacttttat gtttgcggg tcatgatac caccgcaac tggtgtttg tttcgcttt gtatcttct ggccaacaa tccggaagt tcaggaaaa agtattaag tgaacaaaa agaattgtt tggggacga aaaaaatcc ttgtgtgac ttatcaaga gttacaaaa tatgaaata tttggagta cgtcatcaa ggaaacact tagactgta cccctcagt gccagtcat tggaagata cttgaaaga agatacaac atttgggga tcgggtgat ttccgcaaa aacaaacgt tgccatatt tatctacgg tattcatcg aaatcccga ttattttcc ggaaccgga aaagtttat tccagaaag attcgaaaa catgaccaa tttgccccc ttatgccta cataccgtt tagtgctgg acccagaaa ttgcatagg ccaaaaatt tgccatgtt ggaaatgaa aagtctgat ttcgaaggt gatccgcca ttttgaatt aaccccagc caatccaca ccacgagtt ggttttagc tgctgaaac cgtcctaaa gtcagccaa tggtattaa aattggact tagagcccg ctcggaaaa taaaatgtg ataattttg ttgttgttt attttccct taaaccctg acaaatgtt acgccactg gtgacagaa cagattaat atttgacag ttgtttgac gtttaaaat gtcacaatg acgtttcaa caataatgt ttacggttc aagttccat tttaacaaa ttaacaatg tacttatta cgaaacata tcaaaatta aattattat tcgcaccgg aacttgtgg ttattaagt aataaaagt gtcagtttg aaaaactcc ccattactc gtacctgcc cttcggttg aaaaacacc caacctgaa gacaatgcc cccacaagt atcaaagat aaactgaag aaggcagtg gctgagtat ggctcgaca gtgatcgtt ttccatgta ggcatttca ttagtttcc ttaggtgtt tgctatgtt ttagtttca aggtttccc caatcattt aagccagag tttgaaata attaattgt tttttagtg gtctggacg caccgaaat taatccaat cggttggaa tcagtggtg agactgcca ccaaagtgg ccacaaatg ctggcacat ttgcagtgg cttacgccg ttcataaag ttttcgctc cagtgagat tggggatca cgctgactg cgaccccct ttatcgtgc ggtatttga ggcaggtgg gcctcctca agcccccga aataaaatt aataattaa gcacaaaat gttacgtta tttatactt ggattttgt aaaaataaa ccttaattg aaaac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
