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Gene information
General information
TC013773 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4459891..4464728
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013773
    iBeetle screen iB_06315

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Around 60 larvae on slide, most hatched. Don't show any obvious defects.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013773 RA
      MYLIAIVIV LISLYLIVK PVKKTGNID DFPEPPSKV 
FFGHALDAK STTEILNVT TNYVKKYGK IVKLRLGPF 
FKALVVTDY KLVEFFLSS NSLLTKSQN YAFARSWLG 
NGLILANGT HWKQHRKIL TPAFHLEIL REYLETFDS 
VGRIFIEKL KSLDNVQSV DLDALVPLC TLDVICETA 
MGTKINAQK GENSEYVSS VKEMCRIIV ARFLSPLKI 
LKCTYWMTH DFYTERKVV NILHSFTSK VIDGRKTNR 
NETEKSGKK KAFLDLLLK FSDIEKLSD RDIREEVDT 
FMFAGHDTT ATGVCFALY LLANNPEVQ EKVLSEQKE 
LFGDEKNPC VTYQELQNM KYLEYVIKE TLRLYPSVP 
VIGRYLKED TTFGDRVIS AKTNVAIFI YGIHRNPDY 
FPEPEKFIP ERFENMTNL PPYAYIPFS AGPRNCIGQ 
KFAMLEMKS LISKVIRHF ELTPANPHH ELVLAAETV 
LKSANGIKI GLRARSENK M
    CDS sequence
    • >TC013773 RA
      atgtactta attgcgatc gttattgtt ttgatttca 
ctgtattta atcgtaaaa ccagtgaaa aaaactgga 
aacatcgat gatttccct gaacccccg tcgaaggta 
tttttcgga catgccttg gacgccaaa tcaaccaca 
gaaattcta aatgtaacg actaactac gtaaaaaaa 
tatgggaaa atcgtcaag ctcaggttg gggcctttc 
tttaaggca cttgtggta acagactac aaactagtc 
gagtttttt ctaagcagc aattccctg ttaaccaaa 
tcacaaaat tatgcattt gcaaggtcg tggcttgga 
aatggatta attttagcc aacggaacg cactggaag 
caacaccgg aaaatcctc acacctgcg tttcatctc 
gaaattttg agagaatat ctcgaaact tttgattca 
gttgggagg atttttata gaaaaatta aaaagtctg 
gataatgtg caaagtgtg gacttggac gctttggtc 
ccactgtgc accctagac gtaatatgt gaaacggca 
atgggcacc aaaatcaac gcccaaaaa ggcgaaaac 
tcggaatat gtgagcagc gtgaaggaa atgtgtcgg 
atcatagtc gcgcgattt ttgtcacct ttgaaaata 
ttgaaatgt acttattgg atgacgcac gatttctac 
actgagcgc aaagtcgta aacattttg cacagtttt 
acttctaaa gtcatcgac ggcagaaaa acgaatcgc 
aatgaaacg gaaaaaagt gggaaaaaa aaggcattt 
ttagacttg ctgttgaag ttttcagat attgaaaaa 
ttgtctgat cgtgatatt cgtgaggaa gtggacact 
tttatgttt gcgggtcat gataccacc gcaactggt 
gtttgtttc gctttgtat cttctggcc aacaatccg 
gaagttcag gaaaaagta ttaagtgaa caaaaagaa 
ttgtttggg gacgaaaaa aatccttgt gtgacttat 
caagagtta caaaatatg aaatatttg gagtacgtc 
atcaaggaa acacttaga ctgtacccc tcagtgcca 
gtcattgga agatacttg aaagaagat acaacattt 
ggggatcgg gtgatttcc gcaaaaaca aacgttgcc 
atatttatc tacggtatt catcgaaat cccgattat 
tttccggaa ccggaaaag tttattcca gaaagattc 
gaaaacatg accaatttg cccccttat gcctacata 
ccgtttagt gctggaccc agaaattgc ataggccaa 
aaatttgcc atgttggaa atgaaaagt ctgatttcg 
aaggtgatc cgccatttt gaattaacc ccagccaat 
ccacaccac gagttggtt ttagctgct gaaaccgtc 
ctaaagtca gccaatggt attaaaatt ggacttaga 
gcccgctcg gaaaataaa atgtga
    mRNA sequence
    • >TC013773 RA
      tggtccgaa attcaggga attagaagc aaagaattg 
taaaaaaca aggttggat tcatcatta agtaaaatt 
cttccacaa ccagctttt acctgacga catccttcc 
tcgcaaaat ttaaccact ctcacgtta agactatga 
acctcttac aattcaatt tttgggtcc ttttgttga 
caaaccatt gttgctaaa agttgaaga aaacaatct 
tgtacaaaa gttgtggtt cgatttttg cttttacca 
cgtcttcga ataaaatca cctgtattt ctatatctt 
taaatcatt cttgaaatg gatttatgc gagatacca 
aacctctga gctcttctg tgataacat cattctttt 
ttatgtaat ttaacctct taaatacgt tgatttcaa 
atgttcttg ttttcttgc ttgtcgtgg catattgtt 
taaatagac ttgaccact aagccccct tgaaaaact 
gtgaataaa ggcgtttga aatgtgatc gttatttta 
ccttttcat tttcttgct gcagaagat aatggaaca 
cagaatttt cgattaaat agtgaccat ctgacgaaa 
ttatttgat aatacacta ttaaagagc aaattaagt 
gatattcgt gatgtttta ggttatgtt attagtaac 
aaattatca gtaacaaaa aaaaaacag tattgccac 
ctgaaacag taattctta atcacctcg tatttacac 
atgtttatt tactagttt gatggtttt tactatttt 
tggatcgaa catgaaaag tgcatcaca agaagacct 
tgatcttta aataacagc cttattgtt gaactttac 
tctaatttc catcaactt agaatgcat tcaagcgtt 
taatcttat ctaactctt ctatacaaa catttttaa 
cgcgattgc aaacatgta cttaattgc gatcgttat 
tgttttgat ttcactgta tttaatcgt aaaaccagt 
gaaaaaaac tggaaacat cgatgattt ccctgaacc 
cccgtcgaa ggtattttt cggacatgc cttggacgc 
caaatcaac cacagaaat tctaaatgt aacgactaa 
ctacgtaaa aaaatatgg gaaaatcgt caagctcag 
gttggggcc tttctttaa ggcacttgt ggtaacaga 
ctacaaact agtcgagtt ttttctaag cagcaattc 
cctgttaac caaatcaca aaattatgc atttgcaag 
gtcgtggct tggaaatgg attaatttt agccaacgg 
aacgcactg gaagcaaca ccggaaaat cctcacacc 
tgcgtttca tctcgaaat tttgagaga atatctcga 
aacttttga ttcagttgg gaggatttt tatagaaaa 
attaaaaag tctggataa tgtgcaaag tgtggactt 
ggacgcttt ggtcccact gtgcaccct agacgtaat 
atgtgaaac ggcaatggg caccaaaat caacgccca 
aaaaggcga aaactcgga atatgtgag cagcgtgaa 
ggaaatgtg tcggatcat agtcgcgcg atttttgtc 
acctttgaa aatattgaa atgtactta ttggatgac 
gcacgattt ctacactga gcgcaaagt cgtaaacat 
tttgcacag ttttacttc taaagtcat cgacggcag 
aaaaacgaa tcgcaatga aacggaaaa aagtgggaa 
aaaaaaggc atttttaga cttgctgtt gaagttttc 
agatattga aaaattgtc tgatcgtga tattcgtga 
ggaagtgga cacttttat gtttgcggg tcatgatac 
caccgcaac tggtgtttg tttcgcttt gtatcttct 
ggccaacaa tccggaagt tcaggaaaa agtattaag 
tgaacaaaa agaattgtt tggggacga aaaaaatcc 
ttgtgtgac ttatcaaga gttacaaaa tatgaaata 
tttggagta cgtcatcaa ggaaacact tagactgta 
cccctcagt gccagtcat tggaagata cttgaaaga 
agatacaac atttgggga tcgggtgat ttccgcaaa 
aacaaacgt tgccatatt tatctacgg tattcatcg 
aaatcccga ttattttcc ggaaccgga aaagtttat 
tccagaaag attcgaaaa catgaccaa tttgccccc 
ttatgccta cataccgtt tagtgctgg acccagaaa 
ttgcatagg ccaaaaatt tgccatgtt ggaaatgaa 
aagtctgat ttcgaaggt gatccgcca ttttgaatt 
aaccccagc caatccaca ccacgagtt ggttttagc 
tgctgaaac cgtcctaaa gtcagccaa tggtattaa 
aattggact tagagcccg ctcggaaaa taaaatgtg 
ataattttg ttgttgttt attttccct taaaccctg 
acaaatgtt acgccactg gtgacagaa cagattaat 
atttgacag ttgtttgac gtttaaaat gtcacaatg 
acgtttcaa caataatgt ttacggttc aagttccat 
tttaacaaa ttaacaatg tacttatta cgaaacata 
tcaaaatta aattattat tcgcaccgg aacttgtgg 
ttattaagt aataaaagt gtcagtttg aaaaactcc 
ccattactc gtacctgcc cttcggttg aaaaacacc 
caacctgaa gacaatgcc cccacaagt atcaaagat 
aaactgaag aaggcagtg gctgagtat ggctcgaca 
gtgatcgtt ttccatgta ggcatttca ttagtttcc 
ttaggtgtt tgctatgtt ttagtttca aggtttccc 
caatcattt aagccagag tttgaaata attaattgt 
tttttagtg gtctggacg caccgaaat taatccaat 
cggttggaa tcagtggtg agactgcca ccaaagtgg 
ccacaaatg ctggcacat ttgcagtgg cttacgccg 
ttcataaag ttttcgctc cagtgagat tggggatca 
cgctgactg cgaccccct ttatcgtgc ggtatttga 
ggcaggtgg gcctcctca agcccccga aataaaatt 
aataattaa gcacaaaat gttacgtta tttatactt 
ggattttgt aaaaataaa ccttaattg aaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY