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Gene information
General information
TC013773 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4459891..4464728
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013773
    iBeetle screen iB_06315

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Around 60 larvae on slide, most hatched. Don't show any obvious defects.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013773 RA
      MYLIAIVIV LISLYLIVK PVKKTGNID DFPEPPSKV 
      FFGHALDAK STTEILNVT TNYVKKYGK IVKLRLGPF 
      FKALVVTDY KLVEFFLSS NSLLTKSQN YAFARSWLG 
      NGLILANGT HWKQHRKIL TPAFHLEIL REYLETFDS 
      VGRIFIEKL KSLDNVQSV DLDALVPLC TLDVICETA 
      MGTKINAQK GENSEYVSS VKEMCRIIV ARFLSPLKI 
      LKCTYWMTH DFYTERKVV NILHSFTSK VIDGRKTNR 
      NETEKSGKK KAFLDLLLK FSDIEKLSD RDIREEVDT 
      FMFAGHDTT ATGVCFALY LLANNPEVQ EKVLSEQKE 
      LFGDEKNPC VTYQELQNM KYLEYVIKE TLRLYPSVP 
      VIGRYLKED TTFGDRVIS AKTNVAIFI YGIHRNPDY 
      FPEPEKFIP ERFENMTNL PPYAYIPFS AGPRNCIGQ 
      KFAMLEMKS LISKVIRHF ELTPANPHH ELVLAAETV 
      LKSANGIKI GLRARSENK M
    CDS sequence
    • >TC013773 RA
      atgtactta attgcgatc gttattgtt ttgatttca 
      ctgtattta atcgtaaaa ccagtgaaa aaaactgga 
      aacatcgat gatttccct gaacccccg tcgaaggta 
      tttttcgga catgccttg gacgccaaa tcaaccaca 
      gaaattcta aatgtaacg actaactac gtaaaaaaa 
      tatgggaaa atcgtcaag ctcaggttg gggcctttc 
      tttaaggca cttgtggta acagactac aaactagtc 
      gagtttttt ctaagcagc aattccctg ttaaccaaa 
      tcacaaaat tatgcattt gcaaggtcg tggcttgga 
      aatggatta attttagcc aacggaacg cactggaag 
      caacaccgg aaaatcctc acacctgcg tttcatctc 
      gaaattttg agagaatat ctcgaaact tttgattca 
      gttgggagg atttttata gaaaaatta aaaagtctg 
      gataatgtg caaagtgtg gacttggac gctttggtc 
      ccactgtgc accctagac gtaatatgt gaaacggca 
      atgggcacc aaaatcaac gcccaaaaa ggcgaaaac 
      tcggaatat gtgagcagc gtgaaggaa atgtgtcgg 
      atcatagtc gcgcgattt ttgtcacct ttgaaaata 
      ttgaaatgt acttattgg atgacgcac gatttctac 
      actgagcgc aaagtcgta aacattttg cacagtttt 
      acttctaaa gtcatcgac ggcagaaaa acgaatcgc 
      aatgaaacg gaaaaaagt gggaaaaaa aaggcattt 
      ttagacttg ctgttgaag ttttcagat attgaaaaa 
      ttgtctgat cgtgatatt cgtgaggaa gtggacact 
      tttatgttt gcgggtcat gataccacc gcaactggt 
      gtttgtttc gctttgtat cttctggcc aacaatccg 
      gaagttcag gaaaaagta ttaagtgaa caaaaagaa 
      ttgtttggg gacgaaaaa aatccttgt gtgacttat 
      caagagtta caaaatatg aaatatttg gagtacgtc 
      atcaaggaa acacttaga ctgtacccc tcagtgcca 
      gtcattgga agatacttg aaagaagat acaacattt 
      ggggatcgg gtgatttcc gcaaaaaca aacgttgcc 
      atatttatc tacggtatt catcgaaat cccgattat 
      tttccggaa ccggaaaag tttattcca gaaagattc 
      gaaaacatg accaatttg cccccttat gcctacata 
      ccgtttagt gctggaccc agaaattgc ataggccaa 
      aaatttgcc atgttggaa atgaaaagt ctgatttcg 
      aaggtgatc cgccatttt gaattaacc ccagccaat 
      ccacaccac gagttggtt ttagctgct gaaaccgtc 
      ctaaagtca gccaatggt attaaaatt ggacttaga 
      gcccgctcg gaaaataaa atgtga
    mRNA sequence
    • >TC013773 RA
      tggtccgaa attcaggga attagaagc aaagaattg 
      taaaaaaca aggttggat tcatcatta agtaaaatt 
      cttccacaa ccagctttt acctgacga catccttcc 
      tcgcaaaat ttaaccact ctcacgtta agactatga 
      acctcttac aattcaatt tttgggtcc ttttgttga 
      caaaccatt gttgctaaa agttgaaga aaacaatct 
      tgtacaaaa gttgtggtt cgatttttg cttttacca 
      cgtcttcga ataaaatca cctgtattt ctatatctt 
      taaatcatt cttgaaatg gatttatgc gagatacca 
      aacctctga gctcttctg tgataacat cattctttt 
      ttatgtaat ttaacctct taaatacgt tgatttcaa 
      atgttcttg ttttcttgc ttgtcgtgg catattgtt 
      taaatagac ttgaccact aagccccct tgaaaaact 
      gtgaataaa ggcgtttga aatgtgatc gttatttta 
      ccttttcat tttcttgct gcagaagat aatggaaca 
      cagaatttt cgattaaat agtgaccat ctgacgaaa 
      ttatttgat aatacacta ttaaagagc aaattaagt 
      gatattcgt gatgtttta ggttatgtt attagtaac 
      aaattatca gtaacaaaa aaaaaacag tattgccac 
      ctgaaacag taattctta atcacctcg tatttacac 
      atgtttatt tactagttt gatggtttt tactatttt 
      tggatcgaa catgaaaag tgcatcaca agaagacct 
      tgatcttta aataacagc cttattgtt gaactttac 
      tctaatttc catcaactt agaatgcat tcaagcgtt 
      taatcttat ctaactctt ctatacaaa catttttaa 
      cgcgattgc aaacatgta cttaattgc gatcgttat 
      tgttttgat ttcactgta tttaatcgt aaaaccagt 
      gaaaaaaac tggaaacat cgatgattt ccctgaacc 
      cccgtcgaa ggtattttt cggacatgc cttggacgc 
      caaatcaac cacagaaat tctaaatgt aacgactaa 
      ctacgtaaa aaaatatgg gaaaatcgt caagctcag 
      gttggggcc tttctttaa ggcacttgt ggtaacaga 
      ctacaaact agtcgagtt ttttctaag cagcaattc 
      cctgttaac caaatcaca aaattatgc atttgcaag 
      gtcgtggct tggaaatgg attaatttt agccaacgg 
      aacgcactg gaagcaaca ccggaaaat cctcacacc 
      tgcgtttca tctcgaaat tttgagaga atatctcga 
      aacttttga ttcagttgg gaggatttt tatagaaaa 
      attaaaaag tctggataa tgtgcaaag tgtggactt 
      ggacgcttt ggtcccact gtgcaccct agacgtaat 
      atgtgaaac ggcaatggg caccaaaat caacgccca 
      aaaaggcga aaactcgga atatgtgag cagcgtgaa 
      ggaaatgtg tcggatcat agtcgcgcg atttttgtc 
      acctttgaa aatattgaa atgtactta ttggatgac 
      gcacgattt ctacactga gcgcaaagt cgtaaacat 
      tttgcacag ttttacttc taaagtcat cgacggcag 
      aaaaacgaa tcgcaatga aacggaaaa aagtgggaa 
      aaaaaaggc atttttaga cttgctgtt gaagttttc 
      agatattga aaaattgtc tgatcgtga tattcgtga 
      ggaagtgga cacttttat gtttgcggg tcatgatac 
      caccgcaac tggtgtttg tttcgcttt gtatcttct 
      ggccaacaa tccggaagt tcaggaaaa agtattaag 
      tgaacaaaa agaattgtt tggggacga aaaaaatcc 
      ttgtgtgac ttatcaaga gttacaaaa tatgaaata 
      tttggagta cgtcatcaa ggaaacact tagactgta 
      cccctcagt gccagtcat tggaagata cttgaaaga 
      agatacaac atttgggga tcgggtgat ttccgcaaa 
      aacaaacgt tgccatatt tatctacgg tattcatcg 
      aaatcccga ttattttcc ggaaccgga aaagtttat 
      tccagaaag attcgaaaa catgaccaa tttgccccc 
      ttatgccta cataccgtt tagtgctgg acccagaaa 
      ttgcatagg ccaaaaatt tgccatgtt ggaaatgaa 
      aagtctgat ttcgaaggt gatccgcca ttttgaatt 
      aaccccagc caatccaca ccacgagtt ggttttagc 
      tgctgaaac cgtcctaaa gtcagccaa tggtattaa 
      aattggact tagagcccg ctcggaaaa taaaatgtg 
      ataattttg ttgttgttt attttccct taaaccctg 
      acaaatgtt acgccactg gtgacagaa cagattaat 
      atttgacag ttgtttgac gtttaaaat gtcacaatg 
      acgtttcaa caataatgt ttacggttc aagttccat 
      tttaacaaa ttaacaatg tacttatta cgaaacata 
      tcaaaatta aattattat tcgcaccgg aacttgtgg 
      ttattaagt aataaaagt gtcagtttg aaaaactcc 
      ccattactc gtacctgcc cttcggttg aaaaacacc 
      caacctgaa gacaatgcc cccacaagt atcaaagat 
      aaactgaag aaggcagtg gctgagtat ggctcgaca 
      gtgatcgtt ttccatgta ggcatttca ttagtttcc 
      ttaggtgtt tgctatgtt ttagtttca aggtttccc 
      caatcattt aagccagag tttgaaata attaattgt 
      tttttagtg gtctggacg caccgaaat taatccaat 
      cggttggaa tcagtggtg agactgcca ccaaagtgg 
      ccacaaatg ctggcacat ttgcagtgg cttacgccg 
      ttcataaag ttttcgctc cagtgagat tggggatca 
      cgctgactg cgaccccct ttatcgtgc ggtatttga 
      ggcaggtgg gcctcctca agcccccga aataaaatt 
      aataattaa gcacaaaat gttacgtta tttatactt 
      ggattttgt aaaaataaa ccttaattg aaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY